BLASTX nr result
ID: Coptis24_contig00004060
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00004060
(1773 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica] 985 0.0
gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp.... 753 0.0
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera] 692 0.0
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana] 618 e-174
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana] 613 e-173
>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
Length = 533
Score = 985 bits (2547), Expect = 0.0
Identities = 489/533 (91%), Positives = 492/533 (92%)
Frame = -1
Query: 1743 MRATHTIISTLMLLFLCNLTCSQSEDDLPSCLTLHGVSNYTTPFISNSDYDRLLYVSMQN 1564
MRATHTIISTLMLLFLCNLTCSQSEDDLPSCL LHGVSNYTTPFISNSDYDRLLYVSMQN
Sbjct: 1 MRATHTIISTLMLLFLCNLTCSQSEDDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQN 60
Query: 1563 QIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVII 1384
QIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVII
Sbjct: 61 QIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVII 120
Query: 1383 DMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSNVLGFSAGYCPTVXXXXXXXXXXX 1204
DMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSS+VLGFSAGYCPTV
Sbjct: 121 DMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSDVLGFSAGYCPTVGSGGHISGGGF 180
Query: 1203 XXXSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXVYAWKLQLLP 1024
SRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIR VYAWKLQLLP
Sbjct: 181 GMMSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRGGGGGVWGVVYAWKLQLLP 240
Query: 1023 VPKHVTVFKLTKHTSEIDEASKLLYKWQFVAPNLDDDFSLAVLNGAEKDGFWLMFLGLYL 844
VPKHVTVFKLTKHTSEIDEASKLLYKWQ VAPNLDDDFSLAVLNGAEKDGFWL FLGLYL
Sbjct: 241 VPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWLTFLGLYL 300
Query: 843 GPKEFAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTK 664
GPKE AVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTK
Sbjct: 301 GPKEVAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTK 360
Query: 663 VDFAKVPIPLEGISGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYI 484
VDFA+VPIPLEGI+GALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYI
Sbjct: 361 VDFAEVPIPLEGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYI 420
Query: 483 VAWDMDEDLNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXX 304
VAWDMDED NSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTN
Sbjct: 421 VAWDMDEDFNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNSSISASKA 480
Query: 303 XXXARTWGEKYFLSNYDRLVEAKTLIDPNNVFSHPQSIPPLHRGVGCRGNSVI 145
ARTWGEKYFLSNYDRLV AKTLIDPNNVFSHPQSIPPLHRGVGCRGN VI
Sbjct: 481 IEIARTWGEKYFLSNYDRLVGAKTLIDPNNVFSHPQSIPPLHRGVGCRGNIVI 533
>gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
Length = 535
Score = 753 bits (1945), Expect = 0.0
Identities = 375/514 (72%), Positives = 417/514 (81%), Gaps = 3/514 (0%)
Frame = -1
Query: 1713 LMLLFLCNLTCSQSEDDLPSCLTLHGVSNYTT-PFISNSD-YDRLLYVSMQNQIFTRSTF 1540
++L+FL N TC+ +DDLPSCLT+HGV NYTT SNSD Y RLLYVSMQNQIFTRSTF
Sbjct: 6 ILLVFLNNFTCAIIDDDLPSCLTIHGVHNYTTHQSTSNSDAYHRLLYVSMQNQIFTRSTF 65
Query: 1539 PRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGV 1360
P+P VIILPES DQL N ISCC RGSWTIRLRSGGHSYEGLSH AD+PFVIID+MNLNG+
Sbjct: 66 PQPRVIILPESMDQLANVISCCTRGSWTIRLRSGGHSYEGLSHIADNPFVIIDLMNLNGI 125
Query: 1359 SIDLDTQTAWAESGATLGEIYHAIGVSSNVLGFSAGYCPTVXXXXXXXXXXXXXXSRKYG 1180
SIDLDTQTAW ESGATLGEIYHAIG SS + FSAGYCPT SRKYG
Sbjct: 126 SIDLDTQTAWVESGATLGEIYHAIGKSSGTMAFSAGYCPTGGSGGHIAPGGFGMMSRKYG 185
Query: 1179 LAADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXVYAWKLQLLPVPKHVTVF 1000
LAADNVVDA+L+ ANG + DR+SMGEDVFWAIR VYAWKLQL+PVPK+VT+F
Sbjct: 186 LAADNVVDALLVDANGVVLDRESMGEDVFWAIRGGGGGVWGAVYAWKLQLVPVPKNVTIF 245
Query: 999 KLTKHTSEIDEASKLLYKWQFVAPNLDDDFSLAVLNGAEKDG-FWLMFLGLYLGPKEFAV 823
+L KH SE+++ASKLL+KWQ VAP L+DDFSLAVL G KD WL FLGLYLGPKE A
Sbjct: 246 RLMKH-SEVEDASKLLHKWQLVAPKLEDDFSLAVLAGTNKDSSIWLTFLGLYLGPKELAS 304
Query: 822 SSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTKVDFAKVP 643
SSMH+KFPELNLL E+C E+SWVEA A+LAGLK V EL +RFL+YDDRAFKTKVDF K
Sbjct: 305 SSMHKKFPELNLLLEDCMEMSWVEATAELAGLKSVSELKDRFLRYDDRAFKTKVDFPKEA 364
Query: 642 IPLEGISGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYIVAWDMDE 463
IPLEGI GAL+ILKKEQRGFMVMNGQGGMM RIS D+ PFPHRSG LSM+EYIVAWD E
Sbjct: 365 IPLEGIQGALEILKKEQRGFMVMNGQGGMMDRISTDASPFPHRSGTLSMVEYIVAWDKHE 424
Query: 462 DLNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXXXXXARTW 283
DL+S+E+I+WLHQ +DYMG+FV NNPRVGYVNHVD D G IDW N ARTW
Sbjct: 425 DLHSNEFIHWLHQLFDYMGKFVSNNPRVGYVNHVDLDLGRIDWVNKTISSGRAIEIARTW 484
Query: 282 GEKYFLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 181
GEKYF+SNYDRLV AKT+IDP NVF+HPQSIPPL
Sbjct: 485 GEKYFMSNYDRLVRAKTMIDPKNVFNHPQSIPPL 518
>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
Length = 536
Score = 692 bits (1786), Expect = 0.0
Identities = 336/510 (65%), Positives = 398/510 (78%), Gaps = 1/510 (0%)
Frame = -1
Query: 1707 LLFLCNLTCSQSEDDLPSCLTLHGVSNYTT-PFISNSDYDRLLYVSMQNQIFTRSTFPRP 1531
L+ +C +TC+ + +L SCLT +GVSN+T S+SDY RLLYVSMQNQIFTR +PRP
Sbjct: 8 LVTICTITCTLGDVNLSSCLTSNGVSNFTALSTSSDSDYHRLLYVSMQNQIFTRPKYPRP 67
Query: 1530 SVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGVSID 1351
S+IILP+SK++L ++ C RG WTIRLRSGGHSYEGLS+ AD+PFV+ID+MNLN +SID
Sbjct: 68 SMIILPQSKEELAASVVCSNRGLWTIRLRSGGHSYEGLSYVADTPFVVIDLMNLNRISID 127
Query: 1350 LDTQTAWAESGATLGEIYHAIGVSSNVLGFSAGYCPTVXXXXXXXXXXXXXXSRKYGLAA 1171
L+++TAW ESGATLGEIY AI +S+ LGFS GYCPTV SRKYGLAA
Sbjct: 128 LESKTAWVESGATLGEIYCAISEASDTLGFSGGYCPTVGSGGHISGGGFGMMSRKYGLAA 187
Query: 1170 DNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXVYAWKLQLLPVPKHVTVFKLT 991
DNV+DA+++ ANGA+ DR SMGEDVFWAIR +YAWKLQLLPVPK VTVFKL
Sbjct: 188 DNVIDALIVDANGAVLDRSSMGEDVFWAIRGGGGGVWGAIYAWKLQLLPVPKQVTVFKLM 247
Query: 990 KHTSEIDEASKLLYKWQFVAPNLDDDFSLAVLNGAEKDGFWLMFLGLYLGPKEFAVSSMH 811
K+ I+EASK+L+KWQ VAP L+DDF+L+VL GA+ +G W FLGLYLGPKE A+SS+
Sbjct: 248 KNFDNIEEASKMLHKWQVVAPALEDDFTLSVLAGADTNGIWFSFLGLYLGPKELAISSVD 307
Query: 810 QKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTKVDFAKVPIPLE 631
Q FPELNL+ E+CKE+SWVE+FA LAGL V+E+NNRFLKYDDRAFKTKVDF K PIPLE
Sbjct: 308 QNFPELNLVMEDCKEMSWVESFAHLAGLNSVEEMNNRFLKYDDRAFKTKVDFVKEPIPLE 367
Query: 630 GISGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYIVAWDMDEDLNS 451
GI GAL +L KE RGFM NGQGG+M RIS DS PFPHR G L M+EYIVAWD DED S
Sbjct: 368 GIKGALTMLTKELRGFMAFNGQGGLMSRISSDSTPFPHRKGTLMMMEYIVAWDRDEDAKS 427
Query: 450 HEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXXXXXARTWGEKY 271
+E+I WLH FY+YMGQF+ ++PR+ YVNHVD D G +DWTN ARTWGEKY
Sbjct: 428 YEFIGWLHGFYNYMGQFLPSDPRIAYVNHVDLDLGRLDWTN-STIASNAIEIARTWGEKY 486
Query: 270 FLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 181
FLSNY+RLV AKTLIDP NVF HPQSIPP+
Sbjct: 487 FLSNYERLVRAKTLIDPKNVFHHPQSIPPM 516
>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
Length = 554
Score = 618 bits (1593), Expect = e-174
Identities = 307/525 (58%), Positives = 387/525 (73%), Gaps = 5/525 (0%)
Frame = -1
Query: 1740 RATHTIISTLMLL----FLCNLTCSQSEDDLPSCLTLHGVSNYTTPFI-SNSDYDRLLYV 1576
+ T + S+L ++ F LTC+ S DD+ SCLT +GV NYTTP SNSDY RL ++
Sbjct: 10 KITKNLYSSLFIIIFFFFFSVLTCALS-DDILSCLTSNGVHNYTTPSSDSNSDYLRLYHL 68
Query: 1575 SMQNQIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSP 1396
S+QN +F +ST +PS+I+LP +K++L NT+ CC RGSWTIRLRSGGHSYEGLS+TAD+P
Sbjct: 69 SIQNPLFEKSTISKPSLIVLPRNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTP 128
Query: 1395 FVIIDMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSNVLGFSAGYCPTVXXXXXXX 1216
FV+ID+MNLN +SID+D++TAW ESGATLGE+Y+AI ++ LGF+AG+CPTV
Sbjct: 129 FVLIDLMNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHIS 188
Query: 1215 XXXXXXXSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXVYAWKL 1036
SRKYGLAADNV D ILI + GA+ DRK MGEDVFWA+R +YAWK+
Sbjct: 189 GGGFGMMSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKI 248
Query: 1035 QLLPVPKHVTVFKLTKHTSEIDEASKLLYKWQFVAPNLDDDFSLAVLNGAEKDGFWLMFL 856
+LLPVPK VTVF++TK+ + I+EAS L++KWQ+VA LDDDF++++L GA + W++FL
Sbjct: 249 KLLPVPKKVTVFRVTKNVN-IEEASFLIHKWQYVADELDDDFTVSILGGANGNEVWVIFL 307
Query: 855 GLYLGPKEFAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRA 676
GL+LG K A S + +KFPEL L+ EE E++W E+FA L+GLK V ELNNRFLK+DDRA
Sbjct: 308 GLHLGCKTVAKSIIDKKFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKFDDRA 367
Query: 675 FKTKVDFAKVPIPLEGISGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSM 496
FKTKVDF K +PLE I G L+IL KE RGF+ +NG GG M +IS D PFPHR G M
Sbjct: 368 FKTKVDFTKETLPLEAIDGLLEILSKEPRGFIALNGFGGKMSKISNDFTPFPHRKGTKLM 427
Query: 495 IEYIVAWDMDEDLNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXX 316
+EYIVAW DE+ S E+ +WL YDYM FV NPRVGYVNH+D D G IDW++
Sbjct: 428 VEYIVAWSKDEESKSDEFFDWLRNIYDYMEMFVSKNPRVGYVNHIDLDLGGIDWSD-KNS 486
Query: 315 XXXXXXXARTWGEKYFLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 181
AR WGEKYFLSNY+RL+ AKTLIDPNNVF+HPQSIPP+
Sbjct: 487 SNNAIEIARNWGEKYFLSNYERLIRAKTLIDPNNVFNHPQSIPPM 531
>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
Length = 536
Score = 613 bits (1581), Expect = e-173
Identities = 300/511 (58%), Positives = 382/511 (74%), Gaps = 1/511 (0%)
Frame = -1
Query: 1710 MLLFLCNLTCSQSEDDLPSCLTLHGVSNYTTPFI-SNSDYDRLLYVSMQNQIFTRSTFPR 1534
+ +F LTC+ S DD+ SCLT +GV NYTTP SNSDY RL ++S+QN +F +ST +
Sbjct: 19 LFIFFSVLTCALS-DDILSCLTSNGVHNYTTPSSDSNSDYLRLFHLSIQNPLFKKSTISK 77
Query: 1533 PSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGVSI 1354
PS+I+LP +K++L NT+ CC RGSWTIRLRSGGHSYEGLS+TAD+PFV+ID+MNLN +SI
Sbjct: 78 PSLIVLPGNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLNRISI 137
Query: 1353 DLDTQTAWAESGATLGEIYHAIGVSSNVLGFSAGYCPTVXXXXXXXXXXXXXXSRKYGLA 1174
D+D++TAW ESGAT+GE+Y+AI S++ GF+AG+CPTV SRKYGLA
Sbjct: 138 DMDSETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHISGGGFGMMSRKYGLA 197
Query: 1173 ADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXVYAWKLQLLPVPKHVTVFKL 994
ADNV D ILI +NGA+ DRK MGEDVFWA+R +YAWK++LLPVPK VTVF++
Sbjct: 198 ADNVEDVILIDSNGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKIKLLPVPKKVTVFRV 257
Query: 993 TKHTSEIDEASKLLYKWQFVAPNLDDDFSLAVLNGAEKDGFWLMFLGLYLGPKEFAVSSM 814
K+ + I+EAS L++KWQ+VA LDDDF++ +L GA +G WL+FLGL+LG K A S M
Sbjct: 258 MKNVN-IEEASFLIHKWQYVADELDDDFTVTILGGANGNGAWLVFLGLHLGCKTVAKSIM 316
Query: 813 HQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTKVDFAKVPIPL 634
+ FPEL L+ EE E++W E+FA L+GLK V ELNNRFLK DD+AFKTKVDF K +PL
Sbjct: 317 DKMFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKLDDKAFKTKVDFTKETLPL 376
Query: 633 EGISGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYIVAWDMDEDLN 454
+ I+G L+IL KE RGF+++N GG M +IS D PFPHR+G M+EYIV+W DE+
Sbjct: 377 KVINGVLEILSKEPRGFILLNSLGGKMSKISNDFTPFPHRNGTKLMVEYIVSWSKDEESK 436
Query: 453 SHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXXXXXARTWGEK 274
S E+ +WL YDYM +FV NPRVGYVN++D D G IDW+N AR WGEK
Sbjct: 437 SDEFFDWLRNIYDYMEEFVSKNPRVGYVNNIDLDLGGIDWSN-KNSSNNAIEIARNWGEK 495
Query: 273 YFLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 181
YFLSNY+RL+ AKTLIDPNN+F+HPQSIPP+
Sbjct: 496 YFLSNYERLIRAKTLIDPNNIFNHPQSIPPM 526