BLASTX nr result
ID: Coptis24_contig00003522
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00003522
(1800 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002272765.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe... 591 e-166
emb|CBI37009.3| unnamed protein product [Vitis vinifera] 586 e-165
ref|XP_002515211.1| conserved hypothetical protein [Ricinus comm... 568 e-159
ref|XP_004158169.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe... 559 e-157
ref|XP_004135966.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe... 559 e-157
>ref|XP_002272765.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog [Vitis
vinifera]
Length = 465
Score = 591 bits (1523), Expect = e-166
Identities = 296/464 (63%), Positives = 359/464 (77%), Gaps = 11/464 (2%)
Frame = +1
Query: 94 RCNFWLPRKNRFCANVPITHSLFCGNHNPNFEQQRVPCPLDPSHSVLKENMEVHIKRCPF 273
RC+FWLP+K RFCAN I S+FCGNHN + + VPCP+DPSHSVL EN+E H+KRCP
Sbjct: 4 RCSFWLPKKKRFCANFRINDSVFCGNHNTRSDAEWVPCPIDPSHSVLSENLEGHMKRCPL 63
Query: 274 LKQAETLRLQPYYNKGINGGSE-ENCSDIQETTSTTISPL------MKRNAVYGMSVTEF 432
LKQA++L QP+Y KGINGG + E+ D + + T++ L MKRNAVY M+V EF
Sbjct: 64 LKQAQSLSSQPFYQKGINGGKDDEDEGDEKGAAAPTLASLENATSLMKRNAVYSMTVPEF 123
Query: 433 LGLINKIRLIHGSACLDIRDSFKMPEVCSVWMKREVDGKLPFQEKHVLQQASILGNMDEF 612
LI+KI+ IH S DIRDS+++PE C +W+KREVD KLPFQEKHV+QQASILGN++EF
Sbjct: 124 SILISKIQSIHSSIRNDIRDSYRVPEACDIWIKREVDRKLPFQEKHVVQQASILGNLEEF 183
Query: 613 GLFSSSTDGVVNRQLEQQQECNGSLRVG--NGVSAVVEFGAGRGYLTQMLADCYGINKVF 786
G+ S+ +Q ++C+ G NGV AVVEFGAGRGYLTQMLADCYGI +VF
Sbjct: 184 GVLEKSSGD------DQMEQCDSDRSSGDDNGVPAVVEFGAGRGYLTQMLADCYGIKRVF 237
Query: 787 LVERRSYKLKADRSLRQKESLILERLRIDIEDLVLSSVESLRGVPYLAVGKHLCGPATDL 966
LVER+SYKLKADRSLRQKESLILERLRIDIEDL L +VESL+GVPYLA+GKHLCGPATDL
Sbjct: 238 LVERKSYKLKADRSLRQKESLILERLRIDIEDLNLKAVESLQGVPYLAIGKHLCGPATDL 297
Query: 967 TLRCCLHAHSSQ-DRVLLASENHLRGLAIATCCHHLCQWKHYINKGYLSELGITKNEFHA 1143
+LRCCL S+Q D V S ++LRGLAIATCCHHLCQWKHYINK YL LGITK++FHA
Sbjct: 298 SLRCCLAEESNQDDAVQCCSGHYLRGLAIATCCHHLCQWKHYINKKYLMNLGITKDDFHA 357
Query: 1144 ITWFTSWAVDADHSSDISDMVDQGFSVDS-INSEIKDSGEDACKIEGIVRGIPALERAVL 1320
ITWFTSWAVDADH SD+SD+ + S +SE K+ ED + IV+ + A+ERAV+
Sbjct: 358 ITWFTSWAVDADHGSDLSDVAGIDILILSCCHSEKKECVEDVGGVAEIVQNMKAMERAVV 417
Query: 1321 GFMCKDIIDIGRLMWIKKHSLGARLVKYVSSRVSPENHLLVAKY 1452
GFMCK+IID+GRLMW+K+H L +LVKYV +SPENHLL+AK+
Sbjct: 418 GFMCKEIIDMGRLMWVKEHGLETQLVKYVPPTISPENHLLIAKH 461
>emb|CBI37009.3| unnamed protein product [Vitis vinifera]
Length = 448
Score = 586 bits (1511), Expect = e-165
Identities = 293/456 (64%), Positives = 352/456 (77%), Gaps = 3/456 (0%)
Frame = +1
Query: 94 RCNFWLPRKNRFCANVPITHSLFCGNHNPNFEQQRVPCPLDPSHSVLKENMEVHIKRCPF 273
RC+FWLP+K RFCAN I S+FCGNHN + + VPCP+DPSHSVL EN+E H+KRCP
Sbjct: 4 RCSFWLPKKKRFCANFRINDSVFCGNHNTRSDAEWVPCPIDPSHSVLSENLEGHMKRCPL 63
Query: 274 LKQAETLRLQPYYNKGINGGSEENCSDIQETTSTTISPLMKRNAVYGMSVTEFLGLINKI 453
LKQA++L QP+Y KGIN + ++ TS LMKRNAVY M+V EF LI+KI
Sbjct: 64 LKQAQSLSSQPFYQKGINAAP--TLASLENATS-----LMKRNAVYSMTVPEFSILISKI 116
Query: 454 RLIHGSACLDIRDSFKMPEVCSVWMKREVDGKLPFQEKHVLQQASILGNMDEFGLFSSST 633
+ IH S DIRDS+++PE C +W+KREVD KLPFQEKHV+QQASILGN++EFG+ S+
Sbjct: 117 QSIHSSIRNDIRDSYRVPEACDIWIKREVDRKLPFQEKHVVQQASILGNLEEFGVLEKSS 176
Query: 634 DGVVNRQLEQQQECNGSLRVG--NGVSAVVEFGAGRGYLTQMLADCYGINKVFLVERRSY 807
+Q ++C+ G NGV AVVEFGAGRGYLTQMLADCYGI +VFLVER+SY
Sbjct: 177 GD------DQMEQCDSDRSSGDDNGVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSY 230
Query: 808 KLKADRSLRQKESLILERLRIDIEDLVLSSVESLRGVPYLAVGKHLCGPATDLTLRCCLH 987
KLKADRSLRQKESLILERLRIDIEDL L +VESL+GVPYLA+GKHLCGPATDL+LRCCL
Sbjct: 231 KLKADRSLRQKESLILERLRIDIEDLNLKAVESLQGVPYLAIGKHLCGPATDLSLRCCLA 290
Query: 988 AHSSQ-DRVLLASENHLRGLAIATCCHHLCQWKHYINKGYLSELGITKNEFHAITWFTSW 1164
S+Q D V S ++LRGLAIATCCHHLCQWKHYINK YL LGITK++FHAITWFTSW
Sbjct: 291 EESNQDDAVQCCSGHYLRGLAIATCCHHLCQWKHYINKKYLMNLGITKDDFHAITWFTSW 350
Query: 1165 AVDADHSSDISDMVDQGFSVDSINSEIKDSGEDACKIEGIVRGIPALERAVLGFMCKDII 1344
AVDADH SD+SD+ + SI E K+ ED + IV+ + A+ERAV+GFMCK+II
Sbjct: 351 AVDADHGSDLSDVAGCRLHLQSI--EKKECVEDVGGVAEIVQNMKAMERAVVGFMCKEII 408
Query: 1345 DIGRLMWIKKHSLGARLVKYVSSRVSPENHLLVAKY 1452
D+GRLMW+K+H L +LVKYV +SPENHLL+AK+
Sbjct: 409 DMGRLMWVKEHGLETQLVKYVPPTISPENHLLIAKH 444
>ref|XP_002515211.1| conserved hypothetical protein [Ricinus communis]
gi|223545691|gb|EEF47195.1| conserved hypothetical
protein [Ricinus communis]
Length = 438
Score = 568 bits (1463), Expect = e-159
Identities = 285/454 (62%), Positives = 342/454 (75%), Gaps = 1/454 (0%)
Frame = +1
Query: 94 RCNFWLPRKNRFCANVPITHSLFCGNHNPNFEQQRVPCPLDPSHSVLKENMEVHIKRCPF 273
RC FWLP+KNR CAN P++ S FCGNH Q +PCP+D SHSVLKEN+E HIKRCP
Sbjct: 5 RCKFWLPKKNRSCANTPLSDSPFCGNHTQRCNGQWIPCPIDSSHSVLKENLEGHIKRCPL 64
Query: 274 LKQAETLRLQPYYNKGINGGSEENCSDIQETTSTTISPLMKRNAVYGMSVTEFLGLINKI 453
LKQ ++L LQP+Y KGIN G E++ I+ MKRNAVY M+V+EF LI KI
Sbjct: 65 LKQTQSLSLQPFYQKGINAGKEDD---------DDITSEMKRNAVYSMTVSEFYKLIKKI 115
Query: 454 RLIHGSACLDIRDSFKMPEVCSVWMKREVDGKLPFQEKHVLQQASILGNMDEFGLFSSST 633
IH S C DI +SFK+PE C++W+KRE+D +LPFQEKHV QQA+ILGN+++FG+ S+
Sbjct: 116 ESIHASLCNDILESFKLPEACNMWIKREIDSQLPFQEKHVKQQAAILGNLEDFGVLKSTL 175
Query: 634 DGVVNRQLEQQQECNGSLRVGNGVSAVVEFGAGRGYLTQMLADCYGINKVFLVERRSYKL 813
V C+ N V AVVEFGAGRGYLTQMLADCYGI +VFLVER+SYKL
Sbjct: 176 GNKV---------CDDDN--ANCVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKL 224
Query: 814 KADRSLRQKESLILERLRIDIEDLVLSSVESLRGVPYLAVGKHLCGPATDLTLRCCLHAH 993
KADRSLRQKESLILERLRIDIEDL L++VESL+GVPYLA+GKHLCGPATDLTLRCC
Sbjct: 225 KADRSLRQKESLILERLRIDIEDLNLNAVESLQGVPYLAIGKHLCGPATDLTLRCCFSKQ 284
Query: 994 SSQDRVLLASEN-HLRGLAIATCCHHLCQWKHYINKGYLSELGITKNEFHAITWFTSWAV 1170
SS+ + S+N LRGLAIATCCHHLCQWKHYINK +++LGITK EFHAITWFTSWAV
Sbjct: 285 SSEHNMGHCSDNDFLRGLAIATCCHHLCQWKHYINKNLIADLGITKEEFHAITWFTSWAV 344
Query: 1171 DADHSSDISDMVDQGFSVDSINSEIKDSGEDACKIEGIVRGIPALERAVLGFMCKDIIDI 1350
DADH SD+S +D F + S+ E G DA +E VR + A++RA+LGFMCK IID+
Sbjct: 345 DADHGSDLS--IDGRFHLQSMEEE--QCGGDADGVEDAVRNMKAVQRAILGFMCKQIIDM 400
Query: 1351 GRLMWIKKHSLGARLVKYVSSRVSPENHLLVAKY 1452
GR++W K+ L A+LVKYV S VSPENHLL+A++
Sbjct: 401 GRMIWAKECGLDAKLVKYVPSVVSPENHLLIARH 434
>ref|XP_004158169.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog
[Cucumis sativus]
Length = 464
Score = 559 bits (1441), Expect = e-157
Identities = 286/461 (62%), Positives = 340/461 (73%), Gaps = 7/461 (1%)
Frame = +1
Query: 88 AIRCNFWLPRKNRFCANVPITHSLFCGNHNPNFEQQRVPCPLDPSHSVLKENMEVHIKRC 267
A RC FWLP+KNRFCA PI S FCGNH+ + VPCP+DPSHSVL+EN++ H+KRC
Sbjct: 2 ASRCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKRC 61
Query: 268 PFLKQAETLRLQPYYNKGINGGSEENCSDI--QETTSTTISPLMKRNAVYGMSVTEFLGL 441
P LKQ ++L QP+Y KGIN G E++ S S +IS MKRN VYGMS +F L
Sbjct: 62 PLLKQTQSLAGQPFYQKGINAGEEDSMSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRL 121
Query: 442 INKIRLIHGSACLDIRDSFKMPEVCSVWMKREVDGKLPFQEKHVLQQASILGNMDEFGLF 621
+ KIR +H C DI+DS+K+ E C++W+K E+D K+PFQEKHVLQQASILGN+DEFG+
Sbjct: 122 LGKIRALHDLICKDIQDSYKIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL 181
Query: 622 SSSTDGVVNRQLEQQQECNGSLRVGNGVSAVVEFGAGRGYLTQMLADCYGINKVFLVERR 801
S+ G EQ++ N V AVVEFGAGRGYLTQMLADCYGI +VFLVER+
Sbjct: 182 SNYDGG------EQREGDRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERK 235
Query: 802 SYKLKADRSLRQKESLILERLRIDIEDLVLSSVESLRGVPYLAVGKHLCGPATDLTLRCC 981
SYKLKADRSLRQKESLILERLRIDIEDL L++VESLR PYLA+GKHLCGPATDL LRCC
Sbjct: 236 SYKLKADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCC 295
Query: 982 L-----HAHSSQDRVLLASENHLRGLAIATCCHHLCQWKHYINKGYLSELGITKNEFHAI 1146
L HA + + R ++ LRGLAIATCCHHLCQW HY NK YL ELGITK EF AI
Sbjct: 296 LTKQPNHAVTERCR----DKSKLRGLAIATCCHHLCQWNHYTNKRYLLELGITKEEFLAI 351
Query: 1147 TWFTSWAVDADHSSDISDMVDQGFSVDSINSEIKDSGEDACKIEGIVRGIPALERAVLGF 1326
TWFTSWAVDA+HS DISD+ D S SE + G C ++ IV+ + +ERAVLGF
Sbjct: 352 TWFTSWAVDANHSEDISDVTDSKTFFQS--SENESDGMIGCMVKDIVKSMNPVERAVLGF 409
Query: 1327 MCKDIIDIGRLMWIKKHSLGARLVKYVSSRVSPENHLLVAK 1449
MCK+IID+GRLMW+K+ L +LVKYV S +SPENHLL+AK
Sbjct: 410 MCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLLIAK 450
>ref|XP_004135966.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog
[Cucumis sativus]
Length = 458
Score = 559 bits (1441), Expect = e-157
Identities = 286/461 (62%), Positives = 340/461 (73%), Gaps = 7/461 (1%)
Frame = +1
Query: 88 AIRCNFWLPRKNRFCANVPITHSLFCGNHNPNFEQQRVPCPLDPSHSVLKENMEVHIKRC 267
A RC FWLP+KNRFCA PI S FCGNH+ + VPCP+DPSHSVL+EN++ H+KRC
Sbjct: 2 ASRCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKRC 61
Query: 268 PFLKQAETLRLQPYYNKGINGGSEENCSDI--QETTSTTISPLMKRNAVYGMSVTEFLGL 441
P LKQ ++L QP+Y KGIN G E++ S S +IS MKRN VYGMS +F L
Sbjct: 62 PLLKQTQSLAGQPFYQKGINAGEEDSMSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRL 121
Query: 442 INKIRLIHGSACLDIRDSFKMPEVCSVWMKREVDGKLPFQEKHVLQQASILGNMDEFGLF 621
+ KIR +H C DI+DS+K+ E C++W+K E+D K+PFQEKHVLQQASILGN+DEFG+
Sbjct: 122 LGKIRALHDLICKDIQDSYKIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVL 181
Query: 622 SSSTDGVVNRQLEQQQECNGSLRVGNGVSAVVEFGAGRGYLTQMLADCYGINKVFLVERR 801
S+ G EQ++ N V AVVEFGAGRGYLTQMLADCYGI +VFLVER+
Sbjct: 182 SNYDGG------EQREGDRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERK 235
Query: 802 SYKLKADRSLRQKESLILERLRIDIEDLVLSSVESLRGVPYLAVGKHLCGPATDLTLRCC 981
SYKLKADRSLRQKESLILERLRIDIEDL L++VESLR PYLA+GKHLCGPATDL LRCC
Sbjct: 236 SYKLKADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCC 295
Query: 982 L-----HAHSSQDRVLLASENHLRGLAIATCCHHLCQWKHYINKGYLSELGITKNEFHAI 1146
L HA + + R ++ LRGLAIATCCHHLCQW HY NK YL ELGITK EF AI
Sbjct: 296 LTKQPNHAVTERCR----DKSKLRGLAIATCCHHLCQWNHYTNKRYLLELGITKEEFLAI 351
Query: 1147 TWFTSWAVDADHSSDISDMVDQGFSVDSINSEIKDSGEDACKIEGIVRGIPALERAVLGF 1326
TWFTSWAVDA+HS DISD+ D S SE + G C ++ IV+ + +ERAVLGF
Sbjct: 352 TWFTSWAVDANHSEDISDVTDSKTFFQS--SENESDGMIGCMVKDIVKSMNPVERAVLGF 409
Query: 1327 MCKDIIDIGRLMWIKKHSLGARLVKYVSSRVSPENHLLVAK 1449
MCK+IID+GRLMW+K+ L +LVKYV S +SPENHLL+AK
Sbjct: 410 MCKEIIDMGRLMWLKECGLETQLVKYVPSTISPENHLLIAK 450