BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00002943
         (1708 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q39522.1|SMT_COPJA RecName: Full=(S)-scoulerine 9-O-methyltra...   721   0.0  
gb|ACL31653.1| (S)-scoulerine 9-O-methyltransferase [Coptis chin...   655   0.0  
gb|AAU20770.1| (S)-scoulerine 9-O-methyltransferase [Thalictrum ...   630   e-178
gb|AFB74611.1| O-methyltransferase 1 [Papaver somniferum]             426   e-117
gb|AFK73709.1| scoulerine-9-O-methyltransferase [Papaver somnife...   424   e-116

>sp|Q39522.1|SMT_COPJA RecName: Full=(S)-scoulerine 9-O-methyltransferase
            gi|758580|dbj|BAA06192.1|
            S-adenosyl-L-methionine:scoulerine 9-O-methyltransferase
            [Coptis japonica]
          Length = 381

 Score =  721 bits (1860), Expect = 0.0
 Identities = 362/369 (98%), Positives = 367/369 (99%)
 Frame = -2

Query: 1686 FSTKSRLLLEVALRTSVDMAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGP 1507
            FSTK +LLLE ALRTSVDMAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGP
Sbjct: 13   FSTKRKLLLEFALRTSVDMAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGP 72

Query: 1506 DAQLSPSDIVAKIPTKNPSAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVAS 1327
            DAQLSPSDIVAKIPTKNPSAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVAS
Sbjct: 73   DAQLSPSDIVAKIPTKNPSAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVAS 132

Query: 1326 EDKVSVVPMLLFTSDRAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFN 1147
            EDKVSVVPMLLFTSD+AVVESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKE+RVNKSFN
Sbjct: 133  EDKVSVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEERVNKSFN 192

Query: 1146 QAMGAGSTIAFDEVFEVYKGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIG 967
            QAMGAGSTIAFDEVF+VYKGFDNLKELVDVGGGIGTSLSNIVAK+PHIRGINFELPHVIG
Sbjct: 193  QAMGAGSTIAFDEVFKVYKGFDNLKELVDVGGGIGTSLSNIVAKHPHIRGINFELPHVIG 252

Query: 966  DAPDYPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIE 787
            DAPDYPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIE
Sbjct: 253  DAPDYPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIE 312

Query: 786  FVLPQVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLH 607
            FVLPQVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLH
Sbjct: 313  FVLPQVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLH 372

Query: 606  VMEFHKINC 580
            VMEFHKINC
Sbjct: 373  VMEFHKINC 381


>gb|ACL31653.1| (S)-scoulerine 9-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  655 bits (1691), Expect = 0.0
 Identities = 333/351 (94%), Positives = 337/351 (96%)
 Frame = -2

Query: 1632 MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPTKNP 1453
            MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAG         I ++ P +NP
Sbjct: 1    MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGQMLNYH-HQISSQNPHENP 59

Query: 1452 SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFTSDRAV 1273
            SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFT D+AV
Sbjct: 60   SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFTPDKAV 119

Query: 1272 VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY 1093
            VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY
Sbjct: 120  VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY 179

Query: 1092 KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG 913
            KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG
Sbjct: 180  KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG 239

Query: 912  VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGNNAESFNALT 733
            VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGN AESFNALT
Sbjct: 240  VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGNIAESFNALT 299

Query: 732  PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHKINC 580
            PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHKINC
Sbjct: 300  PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHKINC 350


>gb|AAU20770.1| (S)-scoulerine 9-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 355

 Score =  630 bits (1624), Expect = e-178
 Identities = 311/348 (89%), Positives = 332/348 (95%)
 Frame = -2

Query: 1632 MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPTKNP 1453
            MA QEGVNYLSGLGLSRLICLPMALRAAIELNVFEII QAGP+AQLSP++IVAKIPTKNP
Sbjct: 1    MALQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIIFQAGPEAQLSPAEIVAKIPTKNP 60

Query: 1452 SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFTSDRAV 1273
            +AAI+LDRILRMLGASSILSV+T K GRVYGL EESRCLVA ++ VSVVPMLLFTSD+AV
Sbjct: 61   NAAIALDRILRMLGASSILSVTTMKDGRVYGLTEESRCLVADKNGVSVVPMLLFTSDKAV 120

Query: 1272 VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY 1093
            VESFYNIKDVVLEEGVIPFDRTHGMDFF YAGKEQ VNKSFNQAMGAGSTIAFDEVF+VY
Sbjct: 121  VESFYNIKDVVLEEGVIPFDRTHGMDFFAYAGKEQSVNKSFNQAMGAGSTIAFDEVFKVY 180

Query: 1092 KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG 913
            KGF +LKELV+VGGGIGTSLSNI+ KYPHI+GINFELPHVI DAP+YPGVEH+ G+MFEG
Sbjct: 181  KGFHDLKELVNVGGGIGTSLSNIIFKYPHIKGINFELPHVIADAPNYPGVEHIAGNMFEG 240

Query: 912  VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGNNAESFNALT 733
            VPNAQNILLKWVLHDWDD+RSIKIL+NCWKALPE GTVIV+EFVLPQ+LGNNAESFNALT
Sbjct: 241  VPNAQNILLKWVLHDWDDERSIKILQNCWKALPEGGTVIVVEFVLPQILGNNAESFNALT 300

Query: 732  PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHK 589
            PDLLMM LNPGGKERTT EFDGLAKAAGFAETKFFPISQGLHVMEFHK
Sbjct: 301  PDLLMMTLNPGGKERTTTEFDGLAKAAGFAETKFFPISQGLHVMEFHK 348


>gb|AFB74611.1| O-methyltransferase 1 [Papaver somniferum]
          Length = 390

 Score =  426 bits (1096), Expect = e-117
 Identities = 221/362 (61%), Positives = 267/362 (73%), Gaps = 16/362 (4%)
 Frame = -2

Query: 1626 AQEGVNYLSGLG-LSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPT--KN 1456
            +  GV YLS    L +LIC+PMALRAA+ELNVF++IS+ G DA++S S+I +K+P    N
Sbjct: 29   SSNGVCYLSETANLGKLICIPMALRAAMELNVFQLISKFGTDAKVSASEIASKMPNAKNN 88

Query: 1455 PSAAISLDRILRMLGASSILSVSTTKSG------------RVYGLNEESRCLVA-SEDKV 1315
            P AA+ LDRILR+LGASSILSVSTTK              ++YGL   S CLV   ED V
Sbjct: 89   PEAAMYLDRILRLLGASSILSVSTTKKSINRGGDDVVVHEKLYGLTNSSCCLVPRQEDGV 148

Query: 1314 SVVPMLLFTSDRAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMG 1135
            S+V  LLFTSD+ VV+SF+ +K VV E+  +PF+  HG   F+YA  E R+N+ FN  M 
Sbjct: 149  SLVEELLFTSDKVVVDSFFKLKCVVEEKDSVPFEVAHGAKIFEYAATEPRMNQVFNDGMA 208

Query: 1134 AGSTIAFDEVFEVYKGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPD 955
              S + F+ VF VY GF ++KEL+DVGGGIGTS+S IVAKYP IRG+NF+LPHVI  AP 
Sbjct: 209  VFSIVVFEAVFRVYDGFLDMKELLDVGGGIGTSVSKIVAKYPLIRGVNFDLPHVISVAPQ 268

Query: 954  YPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLP 775
            YPGVEHV GDMFE VP  QN+LLKWVLHDW D+R +K+LKNCW +LP  G V++IEFVLP
Sbjct: 269  YPGVEHVAGDMFEEVPKGQNMLLKWVLHDWGDERCVKLLKNCWNSLPVGGKVLIIEFVLP 328

Query: 774  QVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEF 595
              LGNNAESFNAL PDLL+MALNPGGKERT  E+D L KAAGF +T   PIS GLHV+EF
Sbjct: 329  NELGNNAESFNALIPDLLLMALNPGGKERTISEYDDLGKAAGFIKTIPIPISNGLHVIEF 388

Query: 594  HK 589
            HK
Sbjct: 389  HK 390


>gb|AFK73709.1| scoulerine-9-O-methyltransferase [Papaver somniferum]
          Length = 390

 Score =  424 bits (1091), Expect = e-116
 Identities = 220/362 (60%), Positives = 266/362 (73%), Gaps = 16/362 (4%)
 Frame = -2

Query: 1626 AQEGVNYLSGLG-LSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPT--KN 1456
            +  GV YLS    L +LIC+PMALRAA+ELNVF++IS+ G DA++S S+I +K+P    N
Sbjct: 29   SSNGVCYLSETANLGKLICIPMALRAAMELNVFQLISKFGTDAKVSASEIASKMPNAKNN 88

Query: 1455 PSAAISLDRILRMLGASSILSVSTTKSG------------RVYGLNEESRCLVA-SEDKV 1315
            P AA+ LDRILR+LGASSILSVSTTK              ++YGL   S CLV   ED V
Sbjct: 89   PEAAMYLDRILRLLGASSILSVSTTKKSINRGGDDVVVHEKLYGLTNSSCCLVPRQEDGV 148

Query: 1314 SVVPMLLFTSDRAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMG 1135
            S+V  LLFTSD+ VV+SF+ +K VV E+  +PF+  HG   F+YA  E R+N+ FN  M 
Sbjct: 149  SLVEELLFTSDKVVVDSFFKLKCVVEEKDSVPFEVAHGAKIFEYAATEPRMNQVFNDGMA 208

Query: 1134 AGSTIAFDEVFEVYKGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPD 955
              S + F+ VF  Y GF ++KEL+DVGGGIGTS+S IVAKYP IRG+NF+LPHVI  AP 
Sbjct: 209  VFSIVVFEAVFRFYDGFLDMKELLDVGGGIGTSVSKIVAKYPLIRGVNFDLPHVISVAPQ 268

Query: 954  YPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLP 775
            YPGVEHV GDMFE VP  QN+LLKWVLHDW D+R +K+LKNCW +LP  G V++IEFVLP
Sbjct: 269  YPGVEHVAGDMFEEVPKGQNMLLKWVLHDWGDERCVKLLKNCWNSLPVGGKVLIIEFVLP 328

Query: 774  QVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEF 595
              LGNNAESFNAL PDLL+MALNPGGKERT  E+D L KAAGF +T   PIS GLHV+EF
Sbjct: 329  NELGNNAESFNALIPDLLLMALNPGGKERTISEYDDLGKAAGFIKTIPIPISNGLHVIEF 388

Query: 594  HK 589
            HK
Sbjct: 389  HK 390