BLASTX nr result
ID: Coptis24_contig00002647
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00002647
(1778 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 485 e-134
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 485 e-134
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 483 e-134
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 483 e-134
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 478 e-132
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 485 bits (1248), Expect = e-134
Identities = 236/452 (52%), Positives = 322/452 (71%), Gaps = 1/452 (0%)
Frame = -1
Query: 1469 IIGNILSLGMKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1290
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 486 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 545
Query: 1289 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLKEMVR 1110
VPH + K+ E N MS+ WA EC + WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 546 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 605
Query: 1109 YLGTKEGKEVNIVEVAFATIINVVSGLCFTKDFIQLDDEKEVGGMKQHIRTIMEVGSAPN 930
++ TKEG+ + + EV FAT++N ++ + ++DFI +D+ + GGMK +R +M +APN
Sbjct: 606 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 665
Query: 929 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 750
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 666 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 724
Query: 749 FSNDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREINSAFVRDSDL 570
+S+D+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI+ + SDL
Sbjct: 725 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 784
Query: 569 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 393
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 785 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 844
Query: 392 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGLDWYLP 213
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H DW LP
Sbjct: 845 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 904
Query: 212 GNICATELDMNEKFGITLLKEQPLVLIPKMRK 117
ELDMN+KFGI L KEQPL++IPK+RK
Sbjct: 905 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 936
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 485 bits (1248), Expect = e-134
Identities = 236/452 (52%), Positives = 322/452 (71%), Gaps = 1/452 (0%)
Frame = -1
Query: 1469 IIGNILSLGMKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1290
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 1289 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLKEMVR 1110
VPH + K+ E N MS+ WA EC + WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 113 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 1109 YLGTKEGKEVNIVEVAFATIINVVSGLCFTKDFIQLDDEKEVGGMKQHIRTIMEVGSAPN 930
++ TKEG+ + + EV FAT++N ++ + ++DFI +D+ + GGMK +R +M +APN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 232
Query: 929 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 750
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 291
Query: 749 FSNDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREINSAFVRDSDL 570
+S+D+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI+ + SDL
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 569 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 393
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 392 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGLDWYLP 213
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H DW LP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 212 GNICATELDMNEKFGITLLKEQPLVLIPKMRK 117
ELDMN+KFGI L KEQPL++IPK+RK
Sbjct: 472 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 503
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 483 bits (1244), Expect = e-134
Identities = 235/452 (51%), Positives = 322/452 (71%), Gaps = 1/452 (0%)
Frame = -1
Query: 1469 IIGNILSLGMKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1290
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 1289 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLKEMVR 1110
VPH + K+ E N MS+ WA EC WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 113 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 1109 YLGTKEGKEVNIVEVAFATIINVVSGLCFTKDFIQLDDEKEVGGMKQHIRTIMEVGSAPN 930
++ TKEG+ + + EV FAT++N ++ + ++DFI +D+ + GGMK +R ++ +APN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 232
Query: 929 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 750
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 291
Query: 749 FSNDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREINSAFVRDSDL 570
+S+D+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI+ + SDL
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 569 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 393
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 392 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGLDWYLP 213
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H DW LP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 212 GNICATELDMNEKFGITLLKEQPLVLIPKMRK 117
ELDMN+KFG+TL KEQPL++IPK+RK
Sbjct: 472 NGTTPDELDMNDKFGVTLQKEQPLLIIPKVRK 503
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 483 bits (1243), Expect = e-134
Identities = 235/453 (51%), Positives = 322/453 (71%), Gaps = 1/453 (0%)
Frame = -1
Query: 1469 IIGNILSLGMKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1290
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 617 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 676
Query: 1289 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLKEMVR 1110
VPH + K+ E N MS+ WA EC WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 677 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 736
Query: 1109 YLGTKEGKEVNIVEVAFATIINVVSGLCFTKDFIQLDDEKEVGGMKQHIRTIMEVGSAPN 930
++ TKEG+ + + EV FAT++N ++ + ++DFI +D+ + GGMK +R ++ +APN
Sbjct: 737 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 796
Query: 929 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 750
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 797 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 855
Query: 749 FSNDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREINSAFVRDSDL 570
+S+D+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI+ + SDL
Sbjct: 856 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 915
Query: 569 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 393
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 916 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 975
Query: 392 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGLDWYLP 213
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H DW LP
Sbjct: 976 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 1035
Query: 212 GNICATELDMNEKFGITLLKEQPLVLIPKMRKQ 114
ELDMN+KFG+TL KEQPL++IPK RK+
Sbjct: 1036 NGTTPDELDMNDKFGVTLQKEQPLLIIPKKRKK 1068
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 478 bits (1231), Expect = e-132
Identities = 229/451 (50%), Positives = 315/451 (69%), Gaps = 1/451 (0%)
Frame = -1
Query: 1469 IIGNILSLGMKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1290
I+GNIL +G PHIS A A+VHGPLI+L+LG++++++ SSP AA EILKTHDR+ S R+
Sbjct: 46 ILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRY 105
Query: 1289 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLKEMVR 1110
+P T + + +++VW C+++WK+LR +CR+ELFS+KAIESQA LRE KL EM+
Sbjct: 106 IPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLD 165
Query: 1109 YLGTKEGKEVNIVEVAFATIINVVSGLCFTKDFIQLDDEKEVGGMKQHIRTIMEVGSAPN 930
+L +K+G+ VNI EV F T N +S L F+KD + +D+ G +K I T+ME+ + PN
Sbjct: 166 FLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCPN 225
Query: 929 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 750
D++P+L LD QG+ +K C+ MF WD+ IKER+E + + + D LDV L+N
Sbjct: 226 IADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRE-RHVKDARKTDFLDVFLSNG 284
Query: 749 FSNDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREINSAFVRDSDL 570
F + +IN + +LF AG+D+T++TVEW MAEL+K V+ +VREEL EI +R+S +
Sbjct: 285 FDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHI 344
Query: 569 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 393
P+L YLNACVKET RLHPP L+P RA E C++MNYTIPK S + VN+WA+GRDP WE
Sbjct: 345 PQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWE 404
Query: 392 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGLDWYLP 213
DPL++ P RFLDSN+DFKG++FE +PF +GRRICPG AS+VH DW L
Sbjct: 405 DPLSYRPERFLDSNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQ 464
Query: 212 GNICATELDMNEKFGITLLKEQPLVLIPKMR 120
LDMN+KF ITL KEQ L+++PK +
Sbjct: 465 NGDDPAMLDMNDKFSITLEKEQHLLVVPKRK 495