BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00002642
         (5281 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis v...  1094   0.0  
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1082   0.0  
dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]                1054   0.0  
dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]      1043   0.0  
dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]               1035   0.0  

>ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis vinifera]
          Length = 883

 Score = 1094 bits (2829), Expect = 0.0
 Identities = 576/887 (64%), Positives = 644/887 (72%), Gaps = 17/887 (1%)
 Frame = +3

Query: 2373 PVVFKRSKTTTKQNQLSAEVNRK-----SSVKSDAPSRKQVSDALSPNGQKSTIQQGKEG 2537
            PV    S+ +     LSA+++ +     S V     S    S     + +KS      E 
Sbjct: 10   PVANGESEDSEDDKPLSAKLSARLPKGNSHVLKGLSSPVLQSQKTKVDVKKSDGDSEDEV 69

Query: 2538 SSSKATAAKTHVTSSNDSTLVADKSIHLGTTLGNNNLEKKYLNKRHAEPT----SDSEDS 2705
              S     KT+V SS+            G +LG+  L    L+    E        S+DS
Sbjct: 70   PLSSKFPLKTYVRSSS------------GKSLGSKGLNSPSLSSHPPETKVGVKESSDDS 117

Query: 2706 DDKMPLSFRLPAGSAAPPKSNSPSVLKGLTXXXXXXXXXEKPKVSVK--SEDSEDDMPLS 2879
            +D++PL  +L   S     S+     KGL+         +K KV+ K  S+DSED++PLS
Sbjct: 118  EDEIPLLLKLQLKSRKGASSSKLLDSKGLSSPASSRPQSQKAKVNNKNLSDDSEDEIPLS 177

Query: 2880 SKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNVKRDNESSQTPKNSNKRPLGDANTSA 3059
            SKF LK+G GAS+ K  + DE KPL+S ++ NG  K+D+E  ++PK  NKRPLG ANTS 
Sbjct: 178  SKFRLKTGMGASLDKLDNFDEKKPLTSTLKPNGISKKDDERVKSPKGQNKRPLGQANTSH 237

Query: 3060 QSSVKKPKPSAVPVSNKVKNASVKAEPXXXXXXXX------VPIAQRMTKSVTSDSKSKP 3221
             SS KKPK     V  KVK  SVKAE                P  +R+ KSV+SD+K K 
Sbjct: 238  HSSSKKPKLLDGSVKGKVKQVSVKAEQKDDDDDDNDDDDEYTPTVKRLKKSVSSDNK-KA 296

Query: 3222 MKQTPXXXXXXXXXXXXXXXXXXXXXXXYLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYK 3401
             K                          Y KSTK PP SGEGQKWTTL HNGVIFPPPYK
Sbjct: 297  SKTKVIPSSRKKATKKAKKSKKLAKSSKYSKSTKVPPNSGEGQKWTTLEHNGVIFPPPYK 356

Query: 3402 PHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKK 3581
            PHGVKM+YNG+PVDLTPEQEEVATMFAVMKDTDYATKP+F+ENFM DWR  LGKNH+IKK
Sbjct: 357  PHGVKMLYNGRPVDLTPEQEEVATMFAVMKDTDYATKPRFIENFMNDWRVRLGKNHVIKK 416

Query: 3582 FELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXXYMWAIVDGVKEKVGNFRVE 3761
            FELCDFTPIYEWH+ EKEKKKQM+T                YMWA+VDGVKEKVGNFRVE
Sbjct: 417  FELCDFTPIYEWHEKEKEKKKQMTTEEKKALKEEKLKQEDKYMWAVVDGVKEKVGNFRVE 476

Query: 3762 PPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAF 3941
            PPGLFRGRGEHPKMGKLKKRI P DITINIGK APVPEC IPGERWKEV+HDNTVTWLAF
Sbjct: 477  PPGLFRGRGEHPKMGKLKKRICPSDITINIGKEAPVPECPIPGERWKEVRHDNTVTWLAF 536

Query: 3942 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAKDFTNKDVVKRQ 4121
            WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLK+YI+NIR  Y KDFTNKD +KRQ
Sbjct: 537  WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKDYIQNIRATYTKDFTNKDTMKRQ 596

Query: 4122 VAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNT 4301
            +AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV LVPPNKLEFDFLGKDS+RYFNT
Sbjct: 597  IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVALVPPNKLEFDFLGKDSIRYFNT 656

Query: 4302 VDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDD 4481
            V+VE+PVYKAIGQFK+GK  GDDLFDKLDTSKLNAHLKELMPGLTAKVFRT+NASITLD+
Sbjct: 657  VEVELPVYKAIGQFKTGKSGGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDE 716

Query: 4482 MLNKETRGGDVAQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXX 4661
            MLN+E RGGD  +KIV+YQHANKEVAIICNHQRSVSKSH  QM +L+EKM          
Sbjct: 717  MLNREARGGDAMEKIVIYQHANKEVAIICNHQRSVSKSHDVQMSRLSEKMRELQGVLDEL 776

Query: 4662 XXXXXRARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALG 4841
                 RA+KGKPPLKD+DGK KKN+TPE LERKIAQTNTKIEKMERD +TKEDLKTVALG
Sbjct: 777  KTDLARAKKGKPPLKDSDGKPKKNMTPEVLERKIAQTNTKIEKMERDMKTKEDLKTVALG 836

Query: 4842 TSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 4982
            TSKINYLDPRISVAWCKR EVPIEKIFNKSLLAKFAWAMDV+P FRF
Sbjct: 837  TSKINYLDPRISVAWCKRQEVPIEKIFNKSLLAKFAWAMDVDPSFRF 883


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1082 bits (2798), Expect = 0.0
 Identities = 571/909 (62%), Positives = 664/909 (73%), Gaps = 19/909 (2%)
 Frame = +3

Query: 2313 MAVDACVKHKFMXXXXXXXAPVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPSRKQVSDAL 2492
            MAV ACVK K M        P+VFKRS  T+K NQL+++  +KSS + D  S +QVSD  
Sbjct: 1    MAVQACVKEKMMDEIDEDDEPLVFKRSNATSKHNQLNSDT-KKSSQRYDGQSGRQVSDVP 59

Query: 2493 SPNGQKSTIQQGKEGSSSKATAAKTHVTSSNDSTLVA---------------DKSIHLGT 2627
            S NGQ S+  +GK   ++K ++ K+ + S   ST  A               D+S H   
Sbjct: 60   STNGQSSSASKGK---TTKLSSMKSPIASPKASTSFAKASPVANPKVSSSSDDRSKHSSK 116

Query: 2628 TLGNNNL-EKKYLNKRHAEPTSDSEDSDDKMPLSFRLPAGSAAPPKSNSPSVLKGLTXXX 2804
                N + E+K L    AEP  DSED   + PLS RL  G     K +S +  KGL    
Sbjct: 117  QNTINVVKEEKELVNPAAEPYGDSED---EKPLSARLFTGLT---KGSSNNANKGLINSS 170

Query: 2805 XXXXXXEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNV 2984
                   KP+++  S+DS+D++PLSSKF LK+ AG S  K Y+SD++K L S  QQNG++
Sbjct: 171  PASLPVPKPEINRYSDDSDDEIPLSSKFRLKANAGTSTVKSYESDDNKTLVSNFQQNGSI 230

Query: 2985 KRDNESSQTPKNSNKRPLGDANTSAQSSVKKPKPS--AVPVSNKVKNASVKAEPXXXXXX 3158
             R ++SS      NKRPLG+  +S QSSVKKPK S  + PV+NK   AS KAEP      
Sbjct: 231  NRGSKSSI---KVNKRPLGEVKSSVQSSVKKPKLSDASTPVNNK--QASKKAEPKADDSD 285

Query: 3159 XX-VPIAQRMTKSVTSDSKSKPMKQTPXXXXXXXXXXXXXXXXXXXXXXXYLKSTKTPPG 3335
               VPI+QRM KS TS +KS   KQ                         Y KS+K PP 
Sbjct: 286  DDHVPISQRMKKSATSGNKSSTAKQKVTKVVSSSMKKASKKFKKVVKKSKYSKSSKVPPS 345

Query: 3336 SGEGQKWTTLVHNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKP 3515
            SGEG+KW TLVHNGVIFPPPYKPHGVKM+Y GKPVDLTPEQEEVATMFAVM DTDY TK 
Sbjct: 346  SGEGKKWNTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKS 405

Query: 3516 KFLENFMTDWRTVLGKNHIIKKFELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXX 3695
            KF ENFM DWR +LGKNH+I+  E CDFTPIYEWHQ EKEKKK+M+              
Sbjct: 406  KFKENFMDDWRKILGKNHVIQNLEDCDFTPIYEWHQREKEKKKKMTAEEKKVLKEERLKQ 465

Query: 3696 XXXYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPE 3875
               YMWAIV+GVKEKVGNFRVEPP LFRGRGEHPKMGKLKK IRP DITINIGK AP+PE
Sbjct: 466  EEKYMWAIVNGVKEKVGNFRVEPPSLFRGRGEHPKMGKLKKCIRPSDITINIGKDAPIPE 525

Query: 3876 CSIPGERWKEVKHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKE 4055
            C IPGE WKE++HDNTVTWLAFWNDPI P+EFKYVFLAASSSLKGQSDKEKYEKARLLK+
Sbjct: 526  CPIPGESWKEIRHDNTVTWLAFWNDPIKPREFKYVFLAASSSLKGQSDKEKYEKARLLKD 585

Query: 4056 YIENIRVNYAKDFTNKDVVKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK 4235
            +I+ IR  Y KDF +KD+ KRQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+
Sbjct: 586  FIQGIRAAYTKDFASKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVE 645

Query: 4236 LVPPNKLEFDFLGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLK 4415
              PP+ L+FDFLGKDS+RY N V+VE+PVYKAI QF++GK+ GDDLFDKLDTSKLNAHLK
Sbjct: 646  PKPPSILKFDFLGKDSIRYQNEVEVELPVYKAIQQFRTGKRGGDDLFDKLDTSKLNAHLK 705

Query: 4416 ELMPGLTAKVFRTFNASITLDDMLNKETRGGDVAQKIVVYQHANKEVAIICNHQRSVSKS 4595
             LMPGLTAKVFRT+NASITLD+ML++ET+GG+VA+KIVVYQHANKEVAIICNHQR+VSKS
Sbjct: 706  GLMPGLTAKVFRTYNASITLDEMLSRETKGGNVAEKIVVYQHANKEVAIICNHQRTVSKS 765

Query: 4596 HSAQMDKLTEKMNXXXXXXXXXXXXXXRARKGKPPLKDADGKTKKNLTPEALERKIAQTN 4775
            H AQM +L  K++              RA+KGKPPL+DADGK K+NLTPEALERKI QTN
Sbjct: 766  HPAQMTRLNGKIDELKGILDGLQTDLARAKKGKPPLEDADGKPKRNLTPEALERKIGQTN 825

Query: 4776 TKIEKMERDRQTKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWA 4955
             KIEKMERD++TKE LKT+ALGTSKI+YLDPRI+VAWCKRHEVPIEK+FNKSLLAKFAW+
Sbjct: 826  AKIEKMERDKETKEGLKTIALGTSKISYLDPRITVAWCKRHEVPIEKVFNKSLLAKFAWS 885

Query: 4956 MDVEPDFRF 4982
            MDV+P FRF
Sbjct: 886  MDVDPSFRF 894


>dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]
          Length = 910

 Score = 1054 bits (2725), Expect = 0.0
 Identities = 558/928 (60%), Positives = 652/928 (70%), Gaps = 38/928 (4%)
 Frame = +3

Query: 2313 MAVDACVKHKFMXXXXXXXAPVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPSRKQVSDAL 2492
            MAV+AC              P+VFKR+ + +KQ+Q ++E  + SS K D  S +Q+ D+ 
Sbjct: 1    MAVEACPTPNLRDDMDEDDEPIVFKRNNSASKQSQTNSETRKVSSQKRDGQSVRQIYDSR 60

Query: 2493 SPNGQKSTIQQGK--------------EGSSSKATAAKTHVTSSNDSTLVADKSIHLG-- 2624
              NG+ S+ Q+G+                  SK +  K+ ++S   ST  A KS      
Sbjct: 61   PANGESSSGQKGRMVPPSKPSQVKSPLSSPPSKPSQVKSPLSSPKVSTSSAKKSPVSSLP 120

Query: 2625 -----TTLG-------NNNLEKKYLNKRHAEPT-------SDSEDSDDKMPLSFRLPAGS 2747
                 +T G       N +     + +  + P        +DSEDSDD  PLS R  A S
Sbjct: 121  ANSKPSTSGSFKVKPFNQHKSATVIKEEKSSPLRLASAIINDSEDSDDGKPLSLRHSASS 180

Query: 2748 AAPPKSNSPSVLKGLTXXXXXXXXXEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKP 2927
            +   K N+  V K             KP+    +EDS+D+ PLSS+F LKSG G S  K 
Sbjct: 181  S---KGNTNQVSK----------EGSKPE----NEDSDDEKPLSSRFPLKSGVGESTSKA 223

Query: 2928 YDSDEDKPLSSKIQQNGNVKRDNESSQTPKNSNKRPLGDANTSAQSSVKKPKPSAVPVSN 3107
            YDSD+DKPL+ K+Q NG+  RD + ++    SNKRP GD  +S Q SVKKPK S+V    
Sbjct: 224  YDSDDDKPLAKKLQHNGSAMRDGQPNKYSNMSNKRPPGDIKSSNQLSVKKPKLSSVTAPT 283

Query: 3108 KVKNASVKAEPXXXXXXXX-VPIAQRMTKSVTSDSKSKPMKQTPXXXXXXXXXXXXXXXX 3284
              K ASVK EP         +PI+QR+ KS TS+SKS  +K+T                 
Sbjct: 284  NKKQASVKPEPKAEDDDDDHLPISQRIKKSPTSESKSSSIKKTTKVVSSSVKKVNKKSKK 343

Query: 3285 XXXXXXXYLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEE 3464
                   Y KS+K PP SGEG+KW  L HNGVIFPPPYKPHG+KM+Y GKPVDLTPEQEE
Sbjct: 344  VMKNSK-YSKSSKVPPSSGEGKKWNKLEHNGVIFPPPYKPHGIKMLYKGKPVDLTPEQEE 402

Query: 3465 VATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKKFELCDFTPIYEWHQNEKEKKK 3644
            VATMFAVM DTDY  KP+F ENF  DW+ +LGKNH+I+  E CDF+PIYEWHQ+EKEKKK
Sbjct: 403  VATMFAVMLDTDYMNKPRFKENFFGDWKKILGKNHVIQNLENCDFSPIYEWHQSEKEKKK 462

Query: 3645 QMSTXXXXXXXXXXXXXXXXYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI 3824
            QM+T                YMWA VDG KEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI
Sbjct: 463  QMTTEEKKALKEEKLKQEEKYMWAWVDGKKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI 522

Query: 3825 RPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAFWNDPINPKEFKYVFLAASSSL 4004
            RP DITINIGK AP+PEC +PGERWKEV+HDNTVTWLAFWNDPINPKEFKYVFLAASS+L
Sbjct: 523  RPCDITINIGKDAPIPECPVPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSTL 582

Query: 4005 KGQSDKEKYEKARLLKEYIENIRVNYAKDFTNKDVVKRQVAVATYLIDKLALRAGNEKDD 4184
            KG SDKEKYEKARLLK+YI+ IR  Y KDF +KD  K+Q+AVATYLIDKLALRAGNEKDD
Sbjct: 583  KGLSDKEKYEKARLLKDYIQGIRAAYTKDFASKDPTKKQIAVATYLIDKLALRAGNEKDD 642

Query: 4185 DEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEG 4364
            DEADTVGCCTLKVENV+ VPPN L+FDFLGKDS+RY N V+VE  V+KAI QF+SGK+  
Sbjct: 643  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVEVEAAVFKAIQQFRSGKEGS 702

Query: 4365 DDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDDMLNKETRGGDVAQKIVVYQHA 4544
             DLFD LDTSKLNAHLKELMPGLTAKVFRT+NASITLDDML++ET+GGDVA+K+VVYQHA
Sbjct: 703  SDLFDLLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETKGGDVAEKVVVYQHA 762

Query: 4545 NKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXXXXXXXRARKGKPPL--KDADG 4718
            NKEVAIICNHQR+VSKSHSAQM +L EK+               R +KGKPPL  K+ADG
Sbjct: 763  NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKAVVEELKSDLSRVKKGKPPLKSKNADG 822

Query: 4719 KTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALGTSKINYLDPRISVAWCKRH 4898
            K K+NL PE+LE+KIAQTN KIEKMERD++TKEDLKTVALGTSKINYLDPRI+VAWCKRH
Sbjct: 823  KPKRNLNPESLEKKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH 882

Query: 4899 EVPIEKIFNKSLLAKFAWAMDVEPDFRF 4982
            EVPIEKIFNKSLLAKF WAMDV+P FRF
Sbjct: 883  EVPIEKIFNKSLLAKFTWAMDVDPSFRF 910


>dbj|BAK32960.1| topoisomerase I [Ophiorrhiza pseudofasciculata]
          Length = 925

 Score = 1043 bits (2696), Expect = 0.0
 Identities = 558/934 (59%), Positives = 651/934 (69%), Gaps = 44/934 (4%)
 Frame = +3

Query: 2313 MAVDACVKHKFMXXXXXXXAPVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPSRKQVSDAL 2492
            MAV+AC  +  M        PV+FKRSK   KQNQ ++E  + S  K D  S    SD  
Sbjct: 1    MAVEACTTN-LMEDMEDDGGPVIFKRSKPAPKQNQANSEKKKLSLQKQDGQSAVHTSDVR 59

Query: 2493 SPNGQKSTIQQGKEGSSSKATAAKTHVTS---SNDSTLVAD---------KSIHLGTTLG 2636
              NG+ S+ Q+G+   S+K    K+ ++S   SN S   +           S    +T  
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 2637 NNNLEKK---YLNKRHAEPT----SDSEDSDDKMPLSFRLPAGSAAPPKSNSPSVLKGLT 2795
              N  K    ++  + + P     S+SE SDD+ PLS R   G ++  KSN  +   G  
Sbjct: 120  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSART-FGCSSKGKSNHANKEGG-- 176

Query: 2796 XXXXXXXXXEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQN 2975
                      +P +  +SEDSED+ PLS +F  KS  G S  K YDSDE KPL++K+QQN
Sbjct: 177  ----DSCSIRRPVIE-ESEDSEDEKPLSCRFTSKSSLGESTSKSYDSDEKKPLAAKVQQN 231

Query: 2976 GNVKRDNESSQTPKNSNKRPLGDANTSAQSSVKKPKPSAVPVSNKVKNASVKAEPXXXXX 3155
            G+  RD   +++   SNKRP G+A +  QSSVKKPK S+   S   K A+VK EP     
Sbjct: 232  GSASRDGPMNKSASLSNKRPPGEAKSLNQSSVKKPKLSSPITSISNKQAAVKPEPKAEDD 291

Query: 3156 XXXVPIAQRMTKSVTSDSKSKP-----------MKQTPXXXXXXXXXXXXXXXXXXXXXX 3302
               +P++QR  KS ++ SK K            +K++P                      
Sbjct: 292  DDNIPLSQRGKKSQSATSKPKDDDDDNVPISQRIKKSPASVSKSSVPVKKASTVVSSSLK 351

Query: 3303 X-------------YLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMIYNGKPVD 3443
                          Y KS+K PPGSGEGQKWTTLVHNGVIFPPPYKPHGVKM+Y  +P+ 
Sbjct: 352  KVNKNSKKVIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMLYKRQPIT 411

Query: 3444 LTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKKFELCDFTPIYEWHQ 3623
            LTPEQEEVATMFAVM DTDY  KP F ENF +DW+ +LGKNH+I+  E CDF PIYEWHQ
Sbjct: 412  LTPEQEEVATMFAVMLDTDYMNKPCFKENFFSDWKKILGKNHMIQNLEDCDFGPIYEWHQ 471

Query: 3624 NEKEKKKQMSTXXXXXXXXXXXXXXXXYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKM 3803
             EKEKKKQM+T                YMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKM
Sbjct: 472  QEKEKKKQMTTEEKKALKEEKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKM 531

Query: 3804 GKLKKRIRPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAFWNDPINPKEFKYVF 3983
            GKLKKRIRPRDITINIGK AP+PEC IPGERWKEV++ NTVTWLA+WNDPIN KEFKYVF
Sbjct: 532  GKLKKRIRPRDITINIGKDAPIPECPIPGERWKEVRNGNTVTWLAYWNDPINQKEFKYVF 591

Query: 3984 LAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAKDFTN-KDVVKRQVAVATYLIDKLAL 4160
            LAASS+LKGQSDKEKYEKARLLK+YI  IR  Y KDFTN KD +K+Q+AVATYLIDKLAL
Sbjct: 592  LAASSTLKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKLAL 651

Query: 4161 RAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNTVDVEVPVYKAIGQ 4340
            RAGNEKDDDEADTVGCCTLKVENV+ VPPN L+FDFLGKDS+RY N V VE  V+KAI Q
Sbjct: 652  RAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAIQQ 711

Query: 4341 FKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDDMLNKETRGGDVAQ 4520
            F+SGK+  +DLFD+LDTSKLNAHLKELMPGLTAKVFRT+NASITLDDML+KET+GG+VA+
Sbjct: 712  FRSGKEGSEDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEVAE 771

Query: 4521 KIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXXXXXXXRARKGKPP 4700
             +VVYQHANKEVAIICNHQR+VSKSHSAQM +L EK++              RA+KGKPP
Sbjct: 772  NVVVYQHANKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTALEELKTDLSRAKKGKPP 831

Query: 4701 LKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALGTSKINYLDPRISV 4880
             K ADGK K+NL PEALERKI QTN KIEKMERD++TKEDLKTVALGTSKINYLDPRI+V
Sbjct: 832  TKGADGKAKRNLNPEALERKITQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITV 891

Query: 4881 AWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 4982
            AWCKRHEVPIEKIFNKSLLAKFAW+MDV+P FRF
Sbjct: 892  AWCKRHEVPIEKIFNKSLLAKFAWSMDVDPSFRF 925


>dbj|BAG31375.1| topoisomerase I [Ophiorrhiza japonica]
          Length = 927

 Score = 1035 bits (2675), Expect = 0.0
 Identities = 557/936 (59%), Positives = 646/936 (69%), Gaps = 46/936 (4%)
 Frame = +3

Query: 2313 MAVDACVKHKFMXXXXXXXAPVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPSRKQVSDAL 2492
            MAV+AC  +  M        PV+FKRS   +KQNQ ++E  + S  K    S    SD  
Sbjct: 1    MAVEACTPN-LMEDMEDDEGPVIFKRSNPASKQNQANSEKKKLSLQKHVGQSAVHTSDVR 59

Query: 2493 SPNGQKSTIQQGKEGSSSKATAAKTHVTS---SNDSTLVAD---------KSIHLGTTLG 2636
              NG+ S+ Q+G+   S+K    K+ ++S   SN S   +           S    +T  
Sbjct: 60   PANGESSSAQKGRIQPSAKTPPVKSPLSSPKASNSSAKASPITSPGANSKPSASATSTAK 119

Query: 2637 NNNLEKK---YLNKRHAEPT----SDSEDSDDKMPLSFRLPAGSAAPPKSNSPSVLKGLT 2795
              N  K    ++  + + P     S+SE SDD+ PLS R   G ++  KSN  +   G +
Sbjct: 120  QQNHHKSSAAFIEPKPSVPAAKANSNSESSDDEKPLSART-FGCSSKGKSNHANKEGGDS 178

Query: 2796 XXXXXXXXXEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQN 2975
                      + +V  +SEDSED+ PLS +F  KS  G S  K YDSDE KPL++K+QQN
Sbjct: 179  CSI-------RRRVIEESEDSEDEKPLSRRFTSKSSLGESTSKSYDSDEKKPLAAKVQQN 231

Query: 2976 GNVKRDNESSQTPKNSNKRPLGDANTSAQSSVKKPKPSAVPVSNKVKNASVKAEPXXXXX 3155
            G+V RD   +++   SNKRP G+  +  QSSVKKPK S+   S   K A+VK EP     
Sbjct: 232  GSVSRDGPMNKSASLSNKRPPGEVKSLNQSSVKKPKLSSPITSISNKQAAVKPEPEPKAE 291

Query: 3156 XXX--------------------------VPIAQRMTKSVTSDSKSKPMKQTPXXXXXXX 3257
                                         VPIAQR+ KS  S SKS    +         
Sbjct: 292  DDDDNIPLSQRGKKSQAATSKPKDEDDDNVPIAQRIKKSPASVSKSSAPVKKASTVVSPS 351

Query: 3258 XXXXXXXXXXXXXXXXYLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMIYNGKP 3437
                            Y KS+K PPGSGEGQKWTTLVHNGVIFPPPY PHGVKM+Y  +P
Sbjct: 352  LKKVNKNSKKVIKKTAYTKSSKVPPGSGEGQKWTTLVHNGVIFPPPYNPHGVKMLYKRQP 411

Query: 3438 VDLTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKKFELCDFTPIYEW 3617
            + LTPEQEEVATMFAVM DTDY  KP+F ENF +DW+ +LGKNH I+  E CDF PIYEW
Sbjct: 412  ITLTPEQEEVATMFAVMLDTDYMNKPRFKENFFSDWKKMLGKNHTIQNLEDCDFGPIYEW 471

Query: 3618 HQNEKEKKKQMSTXXXXXXXXXXXXXXXXYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHP 3797
            HQ EKEKKKQM+T                YMWAIVDGVKEKVGNFRVEPPGLFRGRGEHP
Sbjct: 472  HQQEKEKKKQMTTEEKKALKEDKLQQEEKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHP 531

Query: 3798 KMGKLKKRIRPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAFWNDPINPKEFKY 3977
            KMGKLKKRIRPRDITINIGK AP+PEC IPGERWKEV++DNTVTWLAFW DPIN KEFKY
Sbjct: 532  KMGKLKKRIRPRDITINIGKDAPIPECPIPGERWKEVRNDNTVTWLAFWIDPINQKEFKY 591

Query: 3978 VFLAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAKDFTN-KDVVKRQVAVATYLIDKL 4154
            VFLAASS+LKGQSDKEKYEKARLLK+YI  IR  Y KDFTN KD +K+Q+AVATYLIDKL
Sbjct: 592  VFLAASSTLKGQSDKEKYEKARLLKDYIHGIRAAYTKDFTNNKDPMKKQIAVATYLIDKL 651

Query: 4155 ALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNTVDVEVPVYKAI 4334
            ALRAGNEKDDDEADTVGCCTLKVENV+ VPPN L+FDFLGKDS+RY N V VE  V+KAI
Sbjct: 652  ALRAGNEKDDDEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVQVEPAVFKAI 711

Query: 4335 GQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDDMLNKETRGGDV 4514
             QF+SGK+  +DLFD+LDTSKLNAHLKELMPGLTAKVFRT+NASITLDDML+KET+GG+V
Sbjct: 712  QQFRSGKEGSEDLFDRLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSKETKGGEV 771

Query: 4515 AQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXXXXXXXRARKGK 4694
            A+K+VVYQHANKEVAIICNHQR+VSKSHSAQM +L EK++              R +KGK
Sbjct: 772  AEKVVVYQHANKEVAIICNHQRTVSKSHSAQMSRLNEKIDELKTAMEELKTDLSRVKKGK 831

Query: 4695 PPLKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALGTSKINYLDPRI 4874
            PP K ADGK K+NL PEALERK+AQ N KIEKMERD++TKEDLKTVALGTSKINYLDPRI
Sbjct: 832  PPSKGADGKPKRNLNPEALERKMAQINAKIEKMERDKETKEDLKTVALGTSKINYLDPRI 891

Query: 4875 SVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 4982
            +VAWCKRHEVPIEKIFNKSLLAKFAW+MDV+P FRF
Sbjct: 892  TVAWCKRHEVPIEKIFNKSLLAKFAWSMDVDPSFRF 927