BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00002447
         (2295 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282402.1| PREDICTED: translocon at the outer membrane ...   808   0.0  
ref|XP_004160411.1| PREDICTED: translocon at the outer membrane ...   762   0.0  
ref|XP_002322105.1| amidase family protein [Populus trichocarpa]...   749   0.0  
ref|XP_002530483.1| amidase, putative [Ricinus communis] gi|2235...   746   0.0  
ref|XP_004137345.1| PREDICTED: LOW QUALITY PROTEIN: translocon a...   737   0.0  

>ref|XP_002282402.1| PREDICTED: translocon at the outer membrane of chloroplasts 64 [Vitis
            vinifera] gi|297734325|emb|CBI15572.3| unnamed protein
            product [Vitis vinifera]
          Length = 590

 Score =  808 bits (2088), Expect = 0.0
 Identities = 409/586 (69%), Positives = 474/586 (80%)
 Frame = -3

Query: 2209 ANIFVLLGLGIAGIFLMTRNKLKKVIKEDYGAFVQRIQIXXXXXXXXXXXXXXXXXLTFA 2030
            AN++VLLGLG+AG+ LMTR KLKK I+ED+GAFVQR+Q+                 L+FA
Sbjct: 6    ANLWVLLGLGLAGVLLMTR-KLKKTIREDFGAFVQRLQLLPPPQPAPPKAPHPLTNLSFA 64

Query: 2029 VSDVFDIDGYVTGFGSPDWARAHEAASRTSPVVAAVVEGGATCTGKTIVDELACSINGVN 1850
            VSDVFDI+GYVTGFG+PDWAR H+ ASRTSPVV+A+VEGGATCTGKT+VDE+A SING N
Sbjct: 65   VSDVFDIEGYVTGFGNPDWARTHDTASRTSPVVSALVEGGATCTGKTVVDEMAYSINGEN 124

Query: 1849 KYYGTPTNPAAPAHIPXXXXXXXXXXXXAKLVDFSIGIDTTGGVRVPAAFCGILGFRTSH 1670
            K+YGTPTNPAAP+ IP            A  VDFS+G+DT GGVRVPAAFCG++GFR SH
Sbjct: 125  KHYGTPTNPAAPSRIPGGSSSGAAVAVAANFVDFSLGLDTVGGVRVPAAFCGVIGFRPSH 184

Query: 1669 GALSNIGIIPVSPSLDAVGWFANDPTILRRVGHALLQLPYAVPRAPRNIIIADDCFRLLK 1490
            GA+S++GII VS SLD VGWFA DP+ILRRVGH LLQL YAV R+PR II ADDCF+ L+
Sbjct: 185  GAVSHMGIIHVSSSLDTVGWFAKDPSILRRVGHVLLQLTYAVQRSPRQIIFADDCFQSLR 244

Query: 1489 IPDSRVTPVVTKSVEKVFGKQVLNRVNLDSYLDSKVPSLNQFQNGNRNGELKGSAIRSLA 1310
            IP  RV  VV KS EK+FG+QVL R NL++YLDSKVPSL  F +   NGE + S IRSL 
Sbjct: 245  IPVDRVYQVVLKSTEKLFGRQVLKRENLENYLDSKVPSLKAFHSQKTNGETRNSLIRSLV 304

Query: 1309 NVMLFLQRNEFKSVHGEWIQSAKPDLYPDISTQLRDAVESADDKVHKCQLIRNETRSALN 1130
             +M  LQR+EFK  H EWI S KP L   IS+QL D +E AD ++  CQ  R E RSALN
Sbjct: 305  KIMHCLQRHEFKHNHEEWINSVKPILDASISSQLHDVLEVADTEIENCQSSRMEIRSALN 364

Query: 1129 SLLKDDGVLVIPTVPGPPPKLGAKEILSEDYQSRSNSLLGIASMSGCCQVSVPLGYHESC 950
            SLLKDDG+LVIPTV  PPPKLG KE LSE+YQ+R+  LL IASMSGCCQV+VPLG ++  
Sbjct: 365  SLLKDDGILVIPTVADPPPKLGGKESLSEEYQNRTLGLLSIASMSGCCQVTVPLGLYDKY 424

Query: 949  PVSVSFVAKHGSDRFLLDVVQTMYASLQEQTDIASKSSLSRNTVRQEESAEIAKEKGNNA 770
            PVSVSF+A+HG DRFLLD VQTMYASLQEQ DIA+KS LS N V +E SAEIAKEKGN A
Sbjct: 425  PVSVSFMARHGGDRFLLDTVQTMYASLQEQADIAAKSKLSSNAVDRETSAEIAKEKGNQA 484

Query: 769  FKEKKWQKAIGFYTEAIKLNGNNATYYSNRAAAYLELGSFLQAESDCSTAINLDKKSIKA 590
            FK+K+WQKA+GFYTEAIKL+GNNATYYSNRAAAYLE+GSFLQAE+DC+ AINLDKK++KA
Sbjct: 485  FKDKQWQKAVGFYTEAIKLSGNNATYYSNRAAAYLEMGSFLQAEADCTEAINLDKKNVKA 544

Query: 589  YLRRGTAREMLGYYKEATEDFKHALVLEPTNKRAALSADRLKKLFQ 452
            YLRRGTAREMLGYYK+A EDF++ALVLEPTNKRA+LSADRLKKLFQ
Sbjct: 545  YLRRGTAREMLGYYKDAIEDFRYALVLEPTNKRASLSADRLKKLFQ 590


>ref|XP_004160411.1| PREDICTED: translocon at the outer membrane of chloroplasts 64-like
            [Cucumis sativus]
          Length = 591

 Score =  762 bits (1967), Expect = 0.0
 Identities = 380/585 (64%), Positives = 459/585 (78%)
 Frame = -3

Query: 2209 ANIFVLLGLGIAGIFLMTRNKLKKVIKEDYGAFVQRIQIXXXXXXXXXXXXXXXXXLTFA 2030
            AN++VLLGLG+AGI LMT+ KLKK I+ED+GAF+++ Q+                 LTFA
Sbjct: 6    ANLWVLLGLGLAGILLMTK-KLKKAIREDFGAFIEKFQLLPPPQPAPPKAPHPLTGLTFA 64

Query: 2029 VSDVFDIDGYVTGFGSPDWARAHEAASRTSPVVAAVVEGGATCTGKTIVDELACSINGVN 1850
            VSDVF+I+G+VTGFG PDWA+ H+AASRTSPVV+A+VEGGATC GKT+ +EL+  I+G N
Sbjct: 65   VSDVFEIEGHVTGFGHPDWAKTHDAASRTSPVVSALVEGGATCIGKTVTNELSMGISGEN 124

Query: 1849 KYYGTPTNPAAPAHIPXXXXXXXXXXXXAKLVDFSIGIDTTGGVRVPAAFCGILGFRTSH 1670
            KYY TPTNPA+ + +P            A LVDFS+GIDT G VR+PA+FCGILGFR SH
Sbjct: 125  KYYETPTNPASHSKVPGGSSSGAAVAVAANLVDFSLGIDTVGDVRIPASFCGILGFRPSH 184

Query: 1669 GALSNIGIIPVSPSLDAVGWFANDPTILRRVGHALLQLPYAVPRAPRNIIIADDCFRLLK 1490
            G++S +GI+PVS SLD VG FA DP +LRRVGH LLQLPYAV R PR IIIADDCF+LLK
Sbjct: 185  GSVSQVGIVPVSLSLDTVGMFAKDPNVLRRVGHVLLQLPYAVQRNPRQIIIADDCFQLLK 244

Query: 1489 IPDSRVTPVVTKSVEKVFGKQVLNRVNLDSYLDSKVPSLNQFQNGNRNGELKGSAIRSLA 1310
            IP  R+T  VTKS EK+FG+Q L   NL SYL SKVP+L +      NG L  ++IR LA
Sbjct: 245  IPVDRITQAVTKSTEKLFGRQALKHENLGSYLSSKVPNLKELIGKKTNGNLNSASIRVLA 304

Query: 1309 NVMLFLQRNEFKSVHGEWIQSAKPDLYPDISTQLRDAVESADDKVHKCQLIRNETRSALN 1130
            N+M  ++R EFK  HGEWI S KPDL   +  QL + +E +D  + K + +RNE R A+N
Sbjct: 305  NLMQTVERIEFKGNHGEWIDSVKPDLDSALMEQLNEKLEISDTMIEKFKSVRNEMRMAIN 364

Query: 1129 SLLKDDGVLVIPTVPGPPPKLGAKEILSEDYQSRSNSLLGIASMSGCCQVSVPLGYHESC 950
            SLLKDDGVLVIPT   PPPKLG KEI SE+YQ+R  S L IAS+SGCCQV++PLG+H+SC
Sbjct: 365  SLLKDDGVLVIPTTVDPPPKLGGKEIFSEEYQTRVFSSLSIASISGCCQVTLPLGFHDSC 424

Query: 949  PVSVSFVAKHGSDRFLLDVVQTMYASLQEQTDIASKSSLSRNTVRQEESAEIAKEKGNNA 770
            PVSVSF+A+HG+DRFLLD+VQTMY SLQE+ + ASKS  S+N V QE+SAEIAKEKGN A
Sbjct: 425  PVSVSFLARHGADRFLLDIVQTMYKSLQEEAEAASKSKFSKNAVSQEQSAEIAKEKGNQA 484

Query: 769  FKEKKWQKAIGFYTEAIKLNGNNATYYSNRAAAYLELGSFLQAESDCSTAINLDKKSIKA 590
            +KEK+W+KAIG YTEAIKLN  NATYYSNRAAAYLELG F QAE+DCS AI+LDKK++K+
Sbjct: 485  YKEKQWEKAIGCYTEAIKLNSRNATYYSNRAAAYLELGRFHQAEADCSKAIDLDKKNVKS 544

Query: 589  YLRRGTAREMLGYYKEATEDFKHALVLEPTNKRAALSADRLKKLF 455
            YLRRGTAREMLG+YKEA EDF HALVLEPTNKRA++SA+RL+KLF
Sbjct: 545  YLRRGTAREMLGFYKEAIEDFSHALVLEPTNKRASISAERLRKLF 589


>ref|XP_002322105.1| amidase family protein [Populus trichocarpa]
            gi|222869101|gb|EEF06232.1| amidase family protein
            [Populus trichocarpa]
          Length = 592

 Score =  749 bits (1934), Expect = 0.0
 Identities = 380/587 (64%), Positives = 454/587 (77%), Gaps = 2/587 (0%)
 Frame = -3

Query: 2209 ANIFVLLGLGIAGIFLMTRNKLKKVIKEDYGAFVQRIQIXXXXXXXXXXXXXXXXXLTFA 2030
            AN++VLLGLG+AGIFL T+ K KKV++ED+GAF+ ++Q+                 LTFA
Sbjct: 6    ANLWVLLGLGLAGIFLFTK-KFKKVLREDFGAFIHKLQLLPPPQPAPPKAPHPLTGLTFA 64

Query: 2029 VSDVFDIDGYVTGFGSPDWARAHEAASRTSPVVAAVVEGGATCTGKTIVDELACSINGVN 1850
            VSD+FDI+GYVTGFG PDWA+ HEAASRTS VV+ +VEGGATC GKT++DELA SI G N
Sbjct: 65   VSDLFDIEGYVTGFGHPDWAKTHEAASRTSVVVSTLVEGGATCVGKTVIDELAYSITGEN 124

Query: 1849 KYYGTPTNPAAPAHIPXXXXXXXXXXXXAKLVDFSIGIDTTGGVRVPAAFCGILGFRTSH 1670
            K+YGTPTNP  PA +P            A LVDFS+G+DT GGVRVPA FCGI+GFR S+
Sbjct: 125  KHYGTPTNPVEPARVPGGSSSGAAVAVAANLVDFSLGVDTVGGVRVPAGFCGIIGFRPSY 184

Query: 1669 GALSNIGIIPVSPSLDAVGWFANDPTILRRVGHALLQLPYAVPRAPRNIIIADDCFRLLK 1490
            GA+S IG++PVS SLD VGWFA DP ILRRVGH LLQ  +   R+PR II+ADDCF+LLK
Sbjct: 185  GAISKIGVLPVSVSLDTVGWFAKDPNILRRVGHVLLQSAFGGQRSPRQIIMADDCFQLLK 244

Query: 1489 IPDSRVTPVVTKSVEKVFGKQVLNRVNLDSYLDSKVPSLNQFQNGNRNGELKGSAIRSLA 1310
            IP  R+  VV KS EK FG+Q+L    LD YL+SKVPSL +F N   NGE+K S+IR LA
Sbjct: 245  IPVDRIAQVVVKSTEKHFGRQLLKHEILDVYLNSKVPSLKEFHNKKTNGEVKTSSIRLLA 304

Query: 1309 NVMLFLQRNEFKSVHGEWIQSAKPDLYPDISTQLRDAVESADDKVHKCQLIRNETRSALN 1130
            NVM  L R EF+S H EWI + KP L P++S Q+ + +E +D ++  C+ IR E RSA+N
Sbjct: 305  NVMQLLHRYEFRSNHEEWINTVKPILEPNLSAQMNEIMELSDAEIELCKSIREEMRSAIN 364

Query: 1129 SLLKDDGVLVIPTVPGPPPKLGAKEILSEDYQSRSNSLLGIASMSGCCQVSVPLGYHESC 950
            SLLKDDG+LV+PT    PPKLG KEILSE+YQS S  LL IAS+SGCCQV+VPLGY+  C
Sbjct: 365  SLLKDDGILVVPTTAYLPPKLGGKEILSEEYQSSSFGLLSIASLSGCCQVTVPLGYYNKC 424

Query: 949  PVSVSFVAKHGSDRFLLDVVQTMYASLQEQ--TDIASKSSLSRNTVRQEESAEIAKEKGN 776
            PVSVS +A+HGSDRFLLD VQTMY +LQEQ  T + SKSS   N   +E SAE+AKEKGN
Sbjct: 425  PVSVSLIARHGSDRFLLDTVQTMYKTLQEQAETYVNSKSS---NKDSRENSAEMAKEKGN 481

Query: 775  NAFKEKKWQKAIGFYTEAIKLNGNNATYYSNRAAAYLELGSFLQAESDCSTAINLDKKSI 596
             AFKE++WQKAI +Y EAIKLN  NATYYSNRAAAYLELGSF  AE+DCS AINLDKK++
Sbjct: 482  QAFKEQQWQKAISYYNEAIKLNDKNATYYSNRAAAYLELGSFQHAEADCSNAINLDKKNV 541

Query: 595  KAYLRRGTAREMLGYYKEATEDFKHALVLEPTNKRAALSADRLKKLF 455
            KAYLRRGTAREMLGYYK+A EDFK+ALVLEPTNKRA+LSA+RL+K+F
Sbjct: 542  KAYLRRGTAREMLGYYKDAIEDFKYALVLEPTNKRASLSAERLQKVF 588


>ref|XP_002530483.1| amidase, putative [Ricinus communis] gi|223529980|gb|EEF31906.1|
            amidase, putative [Ricinus communis]
          Length = 589

 Score =  746 bits (1925), Expect = 0.0
 Identities = 377/586 (64%), Positives = 455/586 (77%)
 Frame = -3

Query: 2209 ANIFVLLGLGIAGIFLMTRNKLKKVIKEDYGAFVQRIQIXXXXXXXXXXXXXXXXXLTFA 2030
            AN++VLLGLGIAGI L+T+ KLKKVI+ED+GAFVQ++Q+                 LTFA
Sbjct: 6    ANLWVLLGLGIAGILLVTK-KLKKVIREDFGAFVQKLQLLPPPPPAPPKAPHPLTGLTFA 64

Query: 2029 VSDVFDIDGYVTGFGSPDWARAHEAASRTSPVVAAVVEGGATCTGKTIVDELACSINGVN 1850
            VSDVFDI+GYVTGFG PDWAR HEAAS+TS VV+A+VEGGATC GKT+VDELA SING N
Sbjct: 65   VSDVFDIEGYVTGFGHPDWARTHEAASQTSLVVSALVEGGATCIGKTVVDELAYSINGEN 124

Query: 1849 KYYGTPTNPAAPAHIPXXXXXXXXXXXXAKLVDFSIGIDTTGGVRVPAAFCGILGFRTSH 1670
            ++YGTPTNPA PA +P            A LVDFS+G+DT GGVRVPA FCGI+GFR S+
Sbjct: 125  EHYGTPTNPAVPARVPGGSSSGAAVAVAANLVDFSLGVDTVGGVRVPAGFCGIIGFRPSY 184

Query: 1669 GALSNIGIIPVSPSLDAVGWFANDPTILRRVGHALLQLPYAVPRAPRNIIIADDCFRLLK 1490
            GA+ N GI+P+S SLD VGWFA DP ++RRVGH LLQLP+ V R P+ IIIADDCF+LLK
Sbjct: 185  GAIPNTGILPISASLDTVGWFARDPNVIRRVGHLLLQLPFGVQRNPKQIIIADDCFQLLK 244

Query: 1489 IPDSRVTPVVTKSVEKVFGKQVLNRVNLDSYLDSKVPSLNQFQNGNRNGELKGSAIRSLA 1310
            IP  R+  VV KS EK+FG+QVL    L+  L S+V SL    +   NGEL+ S+IR LA
Sbjct: 245  IPVDRIAQVVIKSTEKIFGRQVLRHEILEDVLFSRVRSLKLLHDQKTNGELRTSSIRLLA 304

Query: 1309 NVMLFLQRNEFKSVHGEWIQSAKPDLYPDISTQLRDAVESADDKVHKCQLIRNETRSALN 1130
            N+M  L R EF+  H EWI   KP++  +IS Q+ + V+  D ++   + IR E R+A+N
Sbjct: 305  NIMRLLHRYEFRHNHEEWINIEKPNVDHNISAQIHEIVDIPDTEIEIFKSIRQEMRAAIN 364

Query: 1129 SLLKDDGVLVIPTVPGPPPKLGAKEILSEDYQSRSNSLLGIASMSGCCQVSVPLGYHESC 950
            SLLKDDG+LVIPT    PPKLG K++LSEDYQ    SLL IAS+SGCCQV++PLG +E C
Sbjct: 365  SLLKDDGILVIPTTAHLPPKLGGKDMLSEDYQILLFSLLSIASLSGCCQVTIPLGLYEKC 424

Query: 949  PVSVSFVAKHGSDRFLLDVVQTMYASLQEQTDIASKSSLSRNTVRQEESAEIAKEKGNNA 770
            PVSVSF+A+HG DRFL+D VQ MY+SLQEQ D+ +K   S  +  QE SAE+AKEKGN A
Sbjct: 425  PVSVSFIARHGGDRFLMDTVQNMYSSLQEQVDMLAKPK-SHTSDNQERSAEMAKEKGNQA 483

Query: 769  FKEKKWQKAIGFYTEAIKLNGNNATYYSNRAAAYLELGSFLQAESDCSTAINLDKKSIKA 590
            +KEK+WQKAI +YTEAIKLNG NATYYSNRAAAYLELGSFLQAE+DC+ AINLDKK++KA
Sbjct: 484  YKEKQWQKAISYYTEAIKLNGKNATYYSNRAAAYLELGSFLQAEADCTKAINLDKKNVKA 543

Query: 589  YLRRGTAREMLGYYKEATEDFKHALVLEPTNKRAALSADRLKKLFQ 452
            YLRRGTAREM+GYYKEA EDF++ALVLEPTNKRAALSA+RL+K+FQ
Sbjct: 544  YLRRGTAREMIGYYKEAIEDFQYALVLEPTNKRAALSAERLRKMFQ 589


>ref|XP_004137345.1| PREDICTED: LOW QUALITY PROTEIN: translocon at the outer membrane of
            chloroplasts 64-like [Cucumis sativus]
          Length = 585

 Score =  737 bits (1903), Expect = 0.0
 Identities = 371/585 (63%), Positives = 451/585 (77%)
 Frame = -3

Query: 2209 ANIFVLLGLGIAGIFLMTRNKLKKVIKEDYGAFVQRIQIXXXXXXXXXXXXXXXXXLTFA 2030
            AN++VLLGLG+AGI LMT+ KLKK I+ED+GAF+++ Q+                 LTFA
Sbjct: 6    ANLWVLLGLGLAGILLMTK-KLKKAIREDFGAFIEKFQLLPPPQPAPPKAPHPLTGLTFA 64

Query: 2029 VSDVFDIDGYVTGFGSPDWARAHEAASRTSPVVAAVVEGGATCTGKTIVDELACSINGVN 1850
            VSDVF+I+G+VTGFG PDWA+ H+AA       +A+VEGGATC GKT+ +EL+  I+G N
Sbjct: 65   VSDVFEIEGHVTGFGHPDWAKTHDAAX------SALVEGGATCIGKTVTNELSMGISGEN 118

Query: 1849 KYYGTPTNPAAPAHIPXXXXXXXXXXXXAKLVDFSIGIDTTGGVRVPAAFCGILGFRTSH 1670
            K+Y TPTNPA+ + +P            A LVDFS+GIDT G VR+PA+FCGILGFR SH
Sbjct: 119  KHYETPTNPASHSKVPGGSSSGAAVAVAANLVDFSLGIDTVGDVRIPASFCGILGFRPSH 178

Query: 1669 GALSNIGIIPVSPSLDAVGWFANDPTILRRVGHALLQLPYAVPRAPRNIIIADDCFRLLK 1490
            G++S +GI+PVS SLD VG FA DP +LRRVGH LLQLPYAV R PR IIIADDCF+LLK
Sbjct: 179  GSVSQVGIVPVSLSLDTVGMFAKDPNVLRRVGHVLLQLPYAVQRNPRQIIIADDCFQLLK 238

Query: 1489 IPDSRVTPVVTKSVEKVFGKQVLNRVNLDSYLDSKVPSLNQFQNGNRNGELKGSAIRSLA 1310
            IP  R+T  VTKS EK+FG+Q L   NL SYL SKVP+L +      NG L  ++IR LA
Sbjct: 239  IPVDRITQAVTKSTEKLFGRQALKHENLGSYLSSKVPNLKELIGKKTNGNLNSASIRVLA 298

Query: 1309 NVMLFLQRNEFKSVHGEWIQSAKPDLYPDISTQLRDAVESADDKVHKCQLIRNETRSALN 1130
            N+M  ++R EFK  HGEWI S KPDL   +  QL + +E +D  + K + +RNE R A+N
Sbjct: 299  NLMQTVERIEFKGNHGEWIDSVKPDLDSALMEQLNEKLEISDTMIEKFKSVRNEMRMAIN 358

Query: 1129 SLLKDDGVLVIPTVPGPPPKLGAKEILSEDYQSRSNSLLGIASMSGCCQVSVPLGYHESC 950
            SLLKDDGVLVIPT    PPKLG KEI SE+YQ+R  S L IAS+SGCCQV++PLG+H+SC
Sbjct: 359  SLLKDDGVLVIPTTVDSPPKLGGKEIFSEEYQTRVFSSLSIASISGCCQVTLPLGFHDSC 418

Query: 949  PVSVSFVAKHGSDRFLLDVVQTMYASLQEQTDIASKSSLSRNTVRQEESAEIAKEKGNNA 770
            PVSVSF+A+HG+DRFLLD+VQTMY SLQE+ + ASKS  S+N V QE+SAEIAKEKGN A
Sbjct: 419  PVSVSFLARHGADRFLLDIVQTMYRSLQEEAEAASKSKFSKNAVSQEQSAEIAKEKGNQA 478

Query: 769  FKEKKWQKAIGFYTEAIKLNGNNATYYSNRAAAYLELGSFLQAESDCSTAINLDKKSIKA 590
            +KEK+W+KAIG YTEAIKLN  NATYYSNRAAAYLELG F QAE+DCS AI+LDKK++K+
Sbjct: 479  YKEKQWEKAIGCYTEAIKLNSRNATYYSNRAAAYLELGRFHQAEADCSKAIDLDKKNVKS 538

Query: 589  YLRRGTAREMLGYYKEATEDFKHALVLEPTNKRAALSADRLKKLF 455
            YLRRGTAREMLG+YKEA EDF HALVLEPTNKRA++SA+RL+KLF
Sbjct: 539  YLRRGTAREMLGFYKEAIEDFSHALVLEPTNKRASISAERLRKLF 583