BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00002315
         (2051 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vini...   777   0.0  
ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|2...   756   0.0  
gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum]   753   0.0  
gb|AFB77929.1| pectin methylesterase [Gossypium hirsutum]             752   0.0  
ref|NP_188048.1| pectinesterase 3 [Arabidopsis thaliana] gi|2298...   749   0.0  

>ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vinifera]
          Length = 611

 Score =  777 bits (2006), Expect = 0.0
 Identities = 384/538 (71%), Positives = 449/538 (83%), Gaps = 4/538 (0%)
 Frame = -3

Query: 1770 SRKKSND----TLSHAILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTI 1603
            SRK S +    T  HA+LKSACS+TRYP+LCYSAIA+VPG + K+++QKDVI  SLN+T+
Sbjct: 80   SRKSSTNDVGLTAGHAVLKSACSSTRYPDLCYSAIATVPGASKKVTSQKDVIAVSLNITV 139

Query: 1602 TAVEHNYFTIKKIIKTRKALTKREKTALHDCLEMIDETLDEIRDTEDELDDYPKKKSLNQ 1423
            TAVEHNYFTI+K++   K LTKREK ALHDCLE IDETLDE+    D+LD+YP KKSL Q
Sbjct: 140  TAVEHNYFTIEKLLDF-KNLTKREKAALHDCLETIDETLDELHVAMDDLDEYPDKKSLTQ 198

Query: 1422 YAEDLKTLVSGAMTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNT 1243
            +A+DLKTL+S AMTNQ++CLDGFS++ ADK VR++L+ GQ HV HM SNALAMI NMT+T
Sbjct: 199  HADDLKTLMSAAMTNQETCLDGFSHDDADKHVREVLLKGQRHVEHMCSNALAMIKNMTDT 258

Query: 1242 DMFNQRSVSGRNLVEEEDVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXADGSGNYRT 1063
            D+  +R    R L+EE D       G+PKWLSAGDRRLL            ADGSG+Y+T
Sbjct: 259  DIAREREAMNRKLMEERD-----ESGWPKWLSAGDRRLLQSSSVTPDVVVAADGSGDYKT 313

Query: 1062 XXXXXXXAPEKSSTRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGS 883
                   APEKSS RYII IKAG Y+ENVE+ KKKTNIMF+GDGR NTIITGS+NVVDGS
Sbjct: 314  VSAAVAAAPEKSSKRYIIGIKAGVYKENVEVGKKKTNIMFLGDGRSNTIITGSKNVVDGS 373

Query: 882  TTFKSATVAAVGERFLARDITFENTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVH 703
            TTF SATVA VGE+F+ARDITF+NTAGPSKHQAVALRVGSDLSAFY+CDMLAYQDTLYVH
Sbjct: 374  TTFNSATVAVVGEKFIARDITFQNTAGPSKHQAVALRVGSDLSAFYKCDMLAYQDTLYVH 433

Query: 702  SLRQFYVKCIVAGTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRDDPNQNTGIVI 523
            S RQFY+ C+VAGTVDFIFGNAAAV QDCD+HARRPNSGQ+NM TAQGR DPNQNTGIVI
Sbjct: 434  SNRQFYINCLVAGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMLTAQGRTDPNQNTGIVI 493

Query: 522  QKCRIGATSDLLPVISSFPSFLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTL 343
            QKCRIGATSDL  VISSF ++LGRPWKEYSRT+VMQ+SI+NVI+PAGW+EW+G+FAL+TL
Sbjct: 494  QKCRIGATSDLQAVISSFKTYLGRPWKEYSRTVVMQTSITNVIDPAGWHEWSGSFALSTL 553

Query: 342  YYAEYQNTGAGAGTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 169
            YY EYQNTGAGAGT+ RV W+G++V+T+A++ Q FT G FI GSSWLGSTGFP+SLGL
Sbjct: 554  YYGEYQNTGAGAGTSKRVTWKGFKVITSASEAQGFTPGTFIAGSSWLGSTGFPYSLGL 611


>ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|222841689|gb|EEE79236.1|
            predicted protein [Populus trichocarpa]
          Length = 579

 Score =  756 bits (1951), Expect = 0.0
 Identities = 370/534 (69%), Positives = 445/534 (83%)
 Frame = -3

Query: 1770 SRKKSNDTLSHAILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTITAVE 1591
            S K S +  +HAILKS+CS+T YPELCYSA+A+VPG  + +++QKDVIE S+NLT  AV+
Sbjct: 50   SHKSSKNEGTHAILKSSCSSTLYPELCYSAVATVPGATSNLASQKDVIELSINLTTKAVQ 109

Query: 1590 HNYFTIKKIIKTRKALTKREKTALHDCLEMIDETLDEIRDTEDELDDYPKKKSLNQYAED 1411
            HN+FT++K+I T+K LTKREKTALHDCLE+IDETLDE+ +   +L+DYP  KSL ++A+D
Sbjct: 110  HNFFTVEKLIATKK-LTKREKTALHDCLEIIDETLDELHEALVDLNDYPNNKSLKKHADD 168

Query: 1410 LKTLVSGAMTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFN 1231
            LKTL+S A+TNQ++CLDGFS+++ADK+VRK L+ GQ+HV  M SN LAMI NMT+TD+ N
Sbjct: 169  LKTLLSSAITNQETCLDGFSHDEADKKVRKALLKGQIHVEKMCSNVLAMIKNMTDTDVAN 228

Query: 1230 QRSVSGRNLVEEEDVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXADGSGNYRTXXXX 1051
            +   + R L++E++ N+ +   +P+W+S  DRRLL            ADGSGNY+T    
Sbjct: 229  ELKTTNRKLMQEKEGNESE---WPEWMSVADRRLLQSSSVTPDVVVAADGSGNYKTVSAA 285

Query: 1050 XXXAPEKSSTRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFK 871
               AP+KSS RYIIRIKAG YRENV++PK KTNIMFMGDGR+ TIIT SRNVVDGSTTF 
Sbjct: 286  VAAAPKKSSKRYIIRIKAGVYRENVDVPKDKTNIMFMGDGRKTTIITASRNVVDGSTTFN 345

Query: 870  SATVAAVGERFLARDITFENTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQ 691
            SATVAAVG+ FLAR ITF+NTAGPSKHQAVALRVGSDLSAFY CDMLAYQDTLYVHS RQ
Sbjct: 346  SATVAAVGQGFLARGITFQNTAGPSKHQAVALRVGSDLSAFYDCDMLAYQDTLYVHSNRQ 405

Query: 690  FYVKCIVAGTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRDDPNQNTGIVIQKCR 511
            F++ C+VAGTVDFIFGNAA VLQDCD+HARRPNSGQ+NM TAQGR DPNQNTGIVIQK R
Sbjct: 406  FFINCLVAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKSR 465

Query: 510  IGATSDLLPVISSFPSFLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAE 331
            IGATSDL PV SSFP++LGRPWKEYSRT++MQSSI++VI PAGW+EW+G+FALNTLYYAE
Sbjct: 466  IGATSDLQPVKSSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWFEWSGSFALNTLYYAE 525

Query: 330  YQNTGAGAGTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 169
            YQN+GAGAGT+ RV W+GYRV+T+A + Q FT G FI GSSWL ST FPFSLGL
Sbjct: 526  YQNSGAGAGTSRRVTWKGYRVITSATEAQRFTPGNFIAGSSWLRSTTFPFSLGL 579


>gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum]
          Length = 576

 Score =  753 bits (1943), Expect = 0.0
 Identities = 369/526 (70%), Positives = 436/526 (82%), Gaps = 1/526 (0%)
 Frame = -3

Query: 1743 SHAILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTIKKI 1564
            +HAI+KSACSNT +PELCYSAI +V   + K+++QKDVIE SLN+T+ AV  NY+ +K++
Sbjct: 58   AHAIVKSACSNTLHPELCYSAIVNVTDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKEL 117

Query: 1563 IKTRKALTKREKTALHDCLEMIDETLDEIRDTEDELDDYPKKKSLNQYAEDLKTLVSGAM 1384
            IKTRK LT REK ALHDCLE +DETLDE+     +L+ YP KKSL ++AEDLKTL+S A+
Sbjct: 118  IKTRKGLTPREKVALHDCLETMDETLDELHTAVADLELYPNKKSLKEHAEDLKTLISSAI 177

Query: 1383 TNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVSG-RN 1207
            TNQ++CLDGFS+++ADK+VRK+L+ GQ HV  M SNALAMICNMTNTD+ N+  +SG R 
Sbjct: 178  TNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTNTDIANEMKLSGSRK 237

Query: 1206 LVEEEDVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXADGSGNYRTXXXXXXXAPEKS 1027
            LVE       D+  +P+WLSAGDRRLL            ADGSG+Y+T       APEKS
Sbjct: 238  LVE-------DNGEWPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVAKAPEKS 290

Query: 1026 STRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAAVG 847
            S RY+IRIKAG YRENV++PKKKTNIMFMGDGR NTIIT SRNV DGSTTF SATVAAVG
Sbjct: 291  SKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITASRNVQDGSTTFHSATVAAVG 350

Query: 846  ERFLARDITFENTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCIVA 667
            E+FLARDITF+NTAG SKHQAVALRVGSDLSAFY+CD+LAYQDTLYVHS RQF+V+C+VA
Sbjct: 351  EKFLARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDTLYVHSNRQFFVQCLVA 410

Query: 666  GTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRDDPNQNTGIVIQKCRIGATSDLL 487
            GTVDFIFGN AAVLQDCD+HARRP SGQ+NM TAQGR DPNQNTGIVIQKCRIGATSDL 
Sbjct: 411  GTVDFIFGNGAAVLQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLR 470

Query: 486  PVISSFPSFLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGA 307
            PV  SFP++LGRPWKEYSRT++MQSSI++VI PAGW+EWNGNFALNTL+Y EY NTGAGA
Sbjct: 471  PVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALNTLFYGEYANTGAGA 530

Query: 306  GTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 169
             T+ RVKW+G++V+T++ + Q +T G FI G SWL STGFPFSLGL
Sbjct: 531  ATSGRVKWKGHKVITSSTEAQAYTPGSFIAGGSWLSSTGFPFSLGL 576


>gb|AFB77929.1| pectin methylesterase [Gossypium hirsutum]
          Length = 582

 Score =  752 bits (1941), Expect = 0.0
 Identities = 374/530 (70%), Positives = 440/530 (83%), Gaps = 1/530 (0%)
 Frame = -3

Query: 1755 NDTLSHAILKSACSNTRYPELCYSAIASVP-GVAAKISTQKDVIEASLNLTITAVEHNYF 1579
            +DT  HA++KSACS T YP+LC+SA+ +VP G A K+ +QKDVIE SLN+T TAVEHNYF
Sbjct: 57   SDTSHHAVVKSACSGTFYPDLCFSAVTTVPAGTAKKVRSQKDVIELSLNITTTAVEHNYF 116

Query: 1578 TIKKIIKTRKALTKREKTALHDCLEMIDETLDEIRDTEDELDDYPKKKSLNQYAEDLKTL 1399
             IKK++  RK LT REKTALHDCLE IDETLDE+ +  ++L +YP KKSL Q+A+DLKTL
Sbjct: 117  KIKKLL-ARKDLTTREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLKTL 175

Query: 1398 VSGAMTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSV 1219
            +S AMTNQ++CLDGFS+E ADK++R++LI G+ +V  M SNALAMI NMT+TD+ N+  +
Sbjct: 176  MSAAMTNQETCLDGFSHEGADKKIREVLIDGEKYVEKMCSNALAMIKNMTDTDIANEMML 235

Query: 1218 SGRNLVEEEDVNKIDSDGFPKWLSAGDRRLLXXXXXXXXXXXXADGSGNYRTXXXXXXXA 1039
               N   +ED + I    +P+WLSAGDRRLL            ADGSGN++T       A
Sbjct: 236  KSSNRKLKEDESGI---AWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFKTVSEAVAKA 292

Query: 1038 PEKSSTRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATV 859
            PEKSS RYIIRIKAG YRENVE+PKKK+NIMF+GDGR  TIITGSRNVVDGSTTF SATV
Sbjct: 293  PEKSSKRYIIRIKAGVYRENVEVPKKKSNIMFIGDGRTKTIITGSRNVVDGSTTFHSATV 352

Query: 858  AAVGERFLARDITFENTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVK 679
            AAVGE+FLARDITF+NTAGPSKHQAVALRVGSDLSAFY CDMLAYQDTLYVHS RQFYV 
Sbjct: 353  AAVGEKFLARDITFQNTAGPSKHQAVALRVGSDLSAFYNCDMLAYQDTLYVHSNRQFYVN 412

Query: 678  CIVAGTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRDDPNQNTGIVIQKCRIGAT 499
            C+VAGTVDFIFGNAAAV Q+CD+HAR+PNSGQ+NM TAQGR DPNQNTGIVIQKCRIGAT
Sbjct: 413  CLVAGTVDFIFGNAAAVFQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGAT 472

Query: 498  SDLLPVISSFPSFLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNT 319
            SDL PV  +FP++LGRPWKEYSRT+VMQS+IS+VI PAGW+EW+G+FAL TL+YAEYQNT
Sbjct: 473  SDLQPVRKNFPTYLGRPWKEYSRTVVMQSTISDVIQPAGWHEWSGSFALKTLFYAEYQNT 532

Query: 318  GAGAGTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 169
            GAGA T+ RVKW GY+V+T+A++ Q FT GRFI G SWL STGFPF+LGL
Sbjct: 533  GAGASTSARVKWGGYKVITSASEAQAFTPGRFIAGGSWLSSTGFPFALGL 582


>ref|NP_188048.1| pectinesterase 3 [Arabidopsis thaliana]
            gi|229891485|sp|O49006.2|PME3_ARATH RecName:
            Full=Pectinesterase/pectinesterase inhibitor 3; Includes:
            RecName: Full=Pectinesterase inhibitor 3; AltName:
            Full=Pectin methylesterase inhibitor 3; Includes:
            RecName: Full=Pectinesterase 3; Short=PE 3; AltName:
            Full=Pectin methylesterase 27; Short=AtPME27; AltName:
            Full=Pectin methylesterase 3; Flags: Precursor
            gi|9279579|dbj|BAB01037.1| pectinesterase [Arabidopsis
            thaliana] gi|14335010|gb|AAK59769.1| AT3g14310/MLN21_9
            [Arabidopsis thaliana] gi|15529256|gb|AAK97722.1|
            AT3g14310/MLN21_9 [Arabidopsis thaliana]
            gi|23506059|gb|AAN28889.1| At3g14310/MLN21_9 [Arabidopsis
            thaliana] gi|332641979|gb|AEE75500.1| pectinesterase 3
            [Arabidopsis thaliana]
          Length = 592

 Score =  749 bits (1935), Expect = 0.0
 Identities = 374/549 (68%), Positives = 439/549 (79%), Gaps = 15/549 (2%)
 Frame = -3

Query: 1770 SRKKSNDTLSHAILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTITAVE 1591
            + K++    SHA+L+S+CS+TRYPELC SA+ +  GV  ++++QKDVIEAS+NLTITAVE
Sbjct: 46   NEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTAGGV--ELTSQKDVIEASVNLTITAVE 103

Query: 1590 HNYFTIKKIIKTRKALTKREKTALHDCLEMIDETLDEIRDTEDELDDYPKKKSLNQYAED 1411
            HNYFT+KK+IK RK LT REKTALHDCLE IDETLDE+ +T ++L  YP KK+L ++A D
Sbjct: 104  HNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGD 163

Query: 1410 LKTLVSGAMTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFN 1231
            LKTL+S A+TNQ++CLDGFS++ ADK+VRK L+ GQ+HV HM SNALAMI NMT+TD+ N
Sbjct: 164  LKTLISSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIAN 223

Query: 1230 -----QRSVSGRNLVEEED----------VNKIDSDGFPKWLSAGDRRLLXXXXXXXXXX 1096
                 + + + R L EE              ++DS+G+P WLSAGDRRLL          
Sbjct: 224  FEQKAKITSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKADAT 283

Query: 1095 XXADGSGNYRTXXXXXXXAPEKSSTRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTI 916
              ADGSG ++T       APE S+ RY+I IKAG YRENVE+ KKK NIMFMGDGR  TI
Sbjct: 284  VAADGSGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTI 343

Query: 915  ITGSRNVVDGSTTFKSATVAAVGERFLARDITFENTAGPSKHQAVALRVGSDLSAFYQCD 736
            ITGSRNVVDGSTTF SATVAAVGERFLARDITF+NTAGPSKHQAVALRVGSD SAFY CD
Sbjct: 344  ITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCD 403

Query: 735  MLAYQDTLYVHSLRQFYVKCIVAGTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGR 556
            MLAYQDTLYVHS RQF+VKC++AGTVDFIFGNAA VLQDCD+HARRPNSGQ+NM TAQGR
Sbjct: 404  MLAYQDTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGR 463

Query: 555  DDPNQNTGIVIQKCRIGATSDLLPVISSFPSFLGRPWKEYSRTIVMQSSISNVINPAGWY 376
             DPNQNTGIVIQKCRIGATSDL  V  SFP++LGRPWKEYS+T++MQS+IS+VI P GW 
Sbjct: 464  TDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWS 523

Query: 375  EWNGNFALNTLYYAEYQNTGAGAGTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGS 196
            EW G FALNTL Y EY NTGAGAGTANRVKWRG++V+T AA+ Q +T G+FI G  WL S
Sbjct: 524  EWTGTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSS 583

Query: 195  TGFPFSLGL 169
            TGFPFSLGL
Sbjct: 584  TGFPFSLGL 592