BLASTX nr result
ID: Coptis24_contig00002162
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00002162
(1750 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|2... 453 e-125
ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor,... 437 e-120
ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2... 432 e-118
ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1... 426 e-116
ref|XP_003551807.1| PREDICTED: aspartic proteinase nepenthesin-2... 423 e-116
>ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|222865783|gb|EEF02914.1|
predicted protein [Populus trichocarpa]
Length = 490
Score = 453 bits (1166), Expect = e-125
Identities = 242/466 (51%), Positives = 294/466 (63%), Gaps = 9/466 (1%)
Frame = +1
Query: 139 LNKAYGTEGTFASNIPHTYHHAIEIQSLLPTTTCSEFSS-RHRGKKGAVLPMFNKNGPCS 315
L K Y EG + H+ H+IE+ SLLP+ +C + A L + +K+GPCS
Sbjct: 29 LEKGYALEGRKVAESHHS--HSIEVSSLLPSASCKPSTKVLSNNDNKASLKVVHKHGPCS 86
Query: 316 PLSNKDNIQKTINHTQILIQDQSRVHSIHSRISK------KGVSITPEEARLPANSGSSL 477
LS +D HT+IL+QDQSRV SIHSR+S K V +T + +PA GS++
Sbjct: 87 KLS-QDEASAAPTHTEILLQDQSRVKSIHSRLSNSKTSGGKDVKVT-DSTTIPAKDGSTV 144
Query: 478 XXXXXXXXXXXXXPKKDLPVIFDTGSDLTWIQCQPCVGGCYPQRDPIFDPIKXXXXXXXX 657
PKKDL +IFDTGSD+TW QCQPC CY Q++ IFDP +
Sbjct: 145 GSGNYIVTVGLGTPKKDLSLIFDTGSDITWTQCQPCARSCYKQKEQIFDPSQSTSYTNIS 204
Query: 658 XXXXXXXQLRXXXXXXXXXXXXXXVYSIQYGDQSYSIGFFGQETLTLSPTDAFPKFKFGC 837
L VY IQYGD S+S+GFFG E LTL+ TDAF FGC
Sbjct: 205 CSSSICNSLTSATGNTPGCASSACVYGIQYGDSSFSVGFFGTEKLTLTSTDAFNNIYFGC 264
Query: 838 GQNNDGLFGKVAGLLGLGRDKISIVSQTALKYKKLFSYCIPAHSS--GFLALGAPPSTAA 1011
GQNN GLFG AGLLGLGRDK+S+VSQTA KY K+FSYC+P+ SS GFL G S A
Sbjct: 265 GQNNQGLFGGSAGLLGLGRDKLSVVSQTAQKYNKIFSYCLPSSSSSTGFLTFGGSASKNA 324
Query: 1012 KFTPLLADSRGPSFYFLNLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSAYSAV 1191
KFTPL S GPSFY L+ GISVGGK LAIS S FS G IIDSGTV+TRLPP+AYSA+
Sbjct: 325 KFTPLSTISAGPSFYGLDFTGISVGGKKLAISASVFSTAGAIIDSGTVITRLPPAAYSAL 384
Query: 1192 RTAFRQAMTTYPSAPALSILDTCYDLSGFKTVSIPKILLHFAGGTDLDIPPTGLLFAQST 1371
R +FR M+ YP ALSILDTCYD S + T+S+PKI F+ G ++DI TG+L+A S
Sbjct: 385 RASFRNLMSKYPMTKALSILDTCYDFSSYTTISVPKIGFSFSSGIEVDIDATGILYASSL 444
Query: 1372 SQACLPFAGNSDAGDVAIIGNKQQQTVEIVFDVAGGKLGFGPGGCS 1509
SQ CL FAGNSDA DV I GN QQ+T+E+ +D + GK+GF PGGCS
Sbjct: 445 SQVCLAFAGNSDATDVFIFGNVQQKTLEVFYDGSAGKVGFAPGGCS 490
>ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor, putative [Ricinus
communis] gi|223545332|gb|EEF46837.1| Aspartic proteinase
nepenthesin-2 precursor, putative [Ricinus communis]
Length = 494
Score = 437 bits (1123), Expect = e-120
Identities = 235/461 (50%), Positives = 289/461 (62%), Gaps = 5/461 (1%)
Frame = +1
Query: 142 NKAYGTEGTFASNIPHTYHHAIEIQSLLPTTTCSEFSSRHRGKKGAVLPMFNKNGPCSPL 321
N Y EG + HT H I + SLLP +C + + A L + +K+GPCS L
Sbjct: 39 NNCYAFEGRKFAESQHT-HTTIHLTSLLPAASCKPSTQVPSIENKAFLKVVHKHGPCSDL 97
Query: 322 SNKDNIQKTINHTQILIQDQSRVHSIHSRISK-KGVSITPEEAR--LPANSGSSLXXXXX 492
+ IL+QDQSRV SIHS++SK G+S A LPA GS +
Sbjct: 98 RQGHKAEAQY----ILLQDQSRVDSIHSKLSKDSGLSDVKATAATTLPAKDGSIIGSGNY 153
Query: 493 XXXXXXXXPKKDLPVIFDTGSDLTWIQCQPCVGGCYPQRDPIFDPIKXXXXXXXXXXXXX 672
PKKD +IFDTGSDLTW QC+PCV CY Q++ IF+P +
Sbjct: 154 FVTVGLGTPKKDFSLIFDTGSDLTWTQCEPCVKSCYNQKEAIFNPSQSTSYANISCGSTL 213
Query: 673 XXQLRXXXXXXXXXXXXXXVYSIQYGDQSYSIGFFGQETLTLSPTDAFPKFKFGCGQNND 852
L VY IQYGD S+SIGFFG+E L+L+ TD F F FGCGQNN
Sbjct: 214 CDSLASATGNIFNCASSTCVYGIQYGDSSFSIGFFGKEKLSLTATDVFNDFYFGCGQNNK 273
Query: 853 GLFGKVAGLLGLGRDKISIVSQTALKYKKLFSYCIPAHSS--GFLALGAPPSTAAKFTPL 1026
GLFG AGLLGLGRDK+S+VSQTA +Y K+FSYC+P+ SS GFL G S +A FTPL
Sbjct: 274 GLFGGAAGLLGLGRDKLSLVSQTAQRYNKIFSYCLPSSSSSTGFLTFGGSTSKSASFTPL 333
Query: 1027 LADSRGPSFYFLNLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSAYSAVRTAFR 1206
S G SFY L+L GISVGG+ LAISPS FS GTIIDSGTV+TRLPP+AYSA+ + FR
Sbjct: 334 ATISGGSSFYGLDLTGISVGGRKLAISPSVFSTAGTIIDSGTVITRLPPAAYSALSSTFR 393
Query: 1207 QAMTTYPSAPALSILDTCYDLSGFKTVSIPKILLHFAGGTDLDIPPTGLLFAQSTSQACL 1386
+ M+ YP+APALSILDTC+D S T+S+PKI L F+GG +DI TG+ + +Q CL
Sbjct: 394 KLMSQYPAAPALSILDTCFDFSNHDTISVPKIGLFFSGGVVVDIDKTGIFYVNDLTQVCL 453
Query: 1387 PFAGNSDAGDVAIIGNKQQQTVEIVFDVAGGKLGFGPGGCS 1509
FAGNSDA DVAI GN QQ+T+E+V+D A G++GF P GCS
Sbjct: 454 AFAGNSDASDVAIFGNVQQKTLEVVYDGAAGRVGFAPAGCS 494
>ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2 [Vitis vinifera]
Length = 481
Score = 432 bits (1110), Expect = e-118
Identities = 227/452 (50%), Positives = 297/452 (65%), Gaps = 7/452 (1%)
Frame = +1
Query: 172 ASNIPHTYHHAIEIQSLLPTTTCSEFSSRHRGKKGAVLPMFNKNGPCSPLSNKDNIQKTI 351
A + P T H+ + I SL+P++ CS S K A L + +K+GPCS LS ++
Sbjct: 34 ALSTPSTLHN-VHITSLMPSSVCSP--SPKGDDKRASLEVIHKHGPCSKLSQDKG--RSP 88
Query: 352 NHTQILIQDQSRVHSIHSRISKK---GVSITPEEARLPANSGSSLXXXXXXXXXXXXXPK 522
+ TQ+L QD+SRV+SI SR++K G + + LP+ SGS++ PK
Sbjct: 89 SRTQMLDQDESRVNSIRSRLAKNPADGGKLKGSKVTLPSKSGSTIGTGNYVVTVGLGTPK 148
Query: 523 KDLPVIFDTGSDLTWIQCQPCVGGCYPQRDPIFDPIKXXXXXXXXXXXXXXXQLRXXXXX 702
+DL IFDTGSDLTW QC+PC CY Q++PIF+P K +L+
Sbjct: 149 RDLTFIFDTGSDLTWTQCEPCARYCYHQQEPIFNPSKSTSYTNISCSSPTCDELKSGTGN 208
Query: 703 XXXXXXXXXVYSIQYGDQSYSIGFFGQETLTLSPTDAFPKFKFGCGQNNDGLFGKVAGLL 882
VY IQYGDQSYS+GFF Q+ L L+ TD F F FGCGQNN GLF VAGL+
Sbjct: 209 SPSCSASTCVYGIQYGDQSYSVGFFAQDKLALTSTDVFNNFLFGCGQNNRGLFVGVAGLI 268
Query: 883 GLGRDKISIVSQTALKYKKLFSYCIPAHSS--GFLALGAPPST--AAKFTPLLADSRGPS 1050
GLGR+ +S+VSQTA KY KLFSYC+P+ SS G+L G+ T A KFTP L +S+GPS
Sbjct: 269 GLGRNALSLVSQTAQKYGKLFSYCLPSTSSSTGYLTFGSGGGTSKAVKFTPSLVNSQGPS 328
Query: 1051 FYFLNLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSAYSAVRTAFRQAMTTYPS 1230
FYFLNL ISVGG+ L+ S S FS GTIIDSGTV++RLPP+AYS +R +F+Q M+ YP
Sbjct: 329 FYFLNLIAISVGGRKLSTSASVFSTAGTIIDSGTVISRLPPTAYSDLRASFQQQMSKYPK 388
Query: 1231 APALSILDTCYDLSGFKTVSIPKILLHFAGGTDLDIPPTGLLFAQSTSQACLPFAGNSDA 1410
A SILDTCYD S + TV +PKI L+F+ G ++D+ P+G+ + + SQ CL FAGNSDA
Sbjct: 389 AAPASILDTCYDFSQYDTVDVPKINLYFSDGAEMDLDPSGIFYILNISQVCLAFAGNSDA 448
Query: 1411 GDVAIIGNKQQQTVEIVFDVAGGKLGFGPGGC 1506
D+AI+GN QQ+T ++V+DVAGG++GF PGGC
Sbjct: 449 TDIAILGNVQQKTFDVVYDVAGGRIGFAPGGC 480
>ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Glycine max]
Length = 488
Score = 426 bits (1095), Expect = e-116
Identities = 227/453 (50%), Positives = 288/453 (63%), Gaps = 9/453 (1%)
Frame = +1
Query: 175 SNIPHTYHHAIEIQSLLPTTTCSEFSSRHRGKKGAVLPMFNKNGPCSPLSNKDNIQKTIN 354
SN H Y H + + SLLP+++CS SS K+ A L + +K+GPCS L+N D K+
Sbjct: 37 SNNLHQYTHLVHLSSLLPSSSCS--SSAKGPKRKASLEVVHKHGPCSQLNNHDGKAKSKT 94
Query: 355 -HTQILIQDQSRVHSIHSRISK---KGVSITP-EEARLPANSGSSLXXXXXXXXXXXXXP 519
H++IL QD+ RV I+SRISK + S++ + LPA SGS + P
Sbjct: 95 PHSEILNQDKERVKYINSRISKNLGQDSSVSELDSVTLPAKSGSLIGSGNYFVVVGLGTP 154
Query: 520 KKDLPVIFDTGSDLTWIQCQPCVGGCYPQRDPIFDPIKXXXXXXXXXXXXXXXQLRXXXX 699
K+DL +IFDTGSDLTW QC+PC CY Q+D IFDP K QL
Sbjct: 155 KRDLSLIFDTGSDLTWTQCEPCARSCYKQQDAIFDPSKSTSYSNITCTSTLCTQLSTATG 214
Query: 700 XXXXXXXXXX--VYSIQYGDQSYSIGFFGQETLTLSPTDAFPKFKFGCGQNNDGLFGKVA 873
+Y IQYGD S+S+G+F +E L+++ TD F FGCGQNN GLFG A
Sbjct: 215 NEPGCSASTKACIYGIQYGDSSFSVGYFSRERLSVTATDIVDNFLFGCGQNNQGLFGGSA 274
Query: 874 GLLGLGRDKISIVSQTALKYKKLFSYCIPAHSS--GFLALGAPPSTAAKFTPLLADSRGP 1047
GL+GLGR IS V QTA Y+K+FSYC+PA SS G L+ G ++ K+TP SRG
Sbjct: 275 GLIGLGRHPISFVQQTAAVYRKIFSYCLPATSSSTGRLSFGTTTTSYVKYTPFSTISRGS 334
Query: 1048 SFYFLNLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSAYSAVRTAFRQAMTTYP 1227
SFY L++ GISVGG L +S S FS GG IIDSGTV+TRLPP+AY+A+R+AFRQ M+ YP
Sbjct: 335 SFYGLDITGISVGGAKLPVSSSTFSTGGAIIDSGTVITRLPPTAYTALRSAFRQGMSKYP 394
Query: 1228 SAPALSILDTCYDLSGFKTVSIPKILLHFAGGTDLDIPPTGLLFAQSTSQACLPFAGNSD 1407
SA LSILDTCYDLSG++ SIPKI FAGG + +PP G+L+ S Q CL FA N D
Sbjct: 395 SAGELSILDTCYDLSGYEVFSIPKIDFSFAGGVTVQLPPQGILYVASAKQVCLAFAANGD 454
Query: 1408 AGDVAIIGNKQQQTVEIVFDVAGGKLGFGPGGC 1506
DV I GN QQ+T+E+V+DV GG++GFG GGC
Sbjct: 455 DSDVTIYGNVQQKTIEVVYDVGGGRIGFGAGGC 487
>ref|XP_003551807.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Glycine max]
Length = 490
Score = 423 bits (1088), Expect = e-116
Identities = 232/455 (50%), Positives = 286/455 (62%), Gaps = 11/455 (2%)
Frame = +1
Query: 175 SNIPHTYHHAIEIQSLLPTTTCSEFSSRHRGKKGAVLPMFNKNGPCSPLSNKDNIQK-TI 351
SN H Y H + + SLLP+++CS SS K A L + +K+GPCS L++ D K T
Sbjct: 38 SNNLHQYTHLVHLSSLLPSSSCS--SSTKGPKTKASLEVVHKHGPCSQLNDHDGKAKSTT 95
Query: 352 NHTQILIQDQSRVHSIHSRISKK-GVSITPEE---ARLPANSGSSLXXXXXXXXXXXXXP 519
H+ IL QD+ RV I+SR+SK G + EE A LPA SGS + P
Sbjct: 96 PHSDILNQDKERVKYINSRLSKNLGQDSSVEELDSATLPAKSGSLIGSGNYFVVVGLGTP 155
Query: 520 KKDLPVIFDTGSDLTWIQCQPCVGGCYPQRDPIFDPIKXXXXXXXXXXXXXXXQLRXXXX 699
K+DL +IFDTGSDLTW QC+PC CY Q+D IFDP K QL
Sbjct: 156 KRDLSLIFDTGSDLTWTQCEPCARSCYKQQDVIFDPSKSTSYSNITCTSALCTQLSTATG 215
Query: 700 XXXXXXXXXX--VYSIQYGDQSYSIGFFGQETLTLSPTDAFPKFKFGCGQNNDGLFGKVA 873
+Y IQYGD S+S+G+F +E LT++ TD F FGCGQNN GLFG A
Sbjct: 216 NDPGCSASTKACIYGIQYGDSSFSVGYFSRERLTVTATDVVDNFLFGCGQNNQGLFGGSA 275
Query: 874 GLLGLGRDKISIVSQTALKYKKLFSYCIPAHSS--GFLALGAPPSTAA--KFTPLLADSR 1041
GL+GLGR IS V QTA KY+K+FSYC+P+ SS G L+ G P +T K+TP SR
Sbjct: 276 GLIGLGRHPISFVQQTAAKYRKIFSYCLPSTSSSTGHLSFG-PAATGRYLKYTPFSTISR 334
Query: 1042 GPSFYFLNLQGISVGGKNLAISPSAFSVGGTIIDSGTVVTRLPPSAYSAVRTAFRQAMTT 1221
G SFY L++ I+VGG L +S S FS GG IIDSGTV+TRLPP+AY A+R+AFRQ M+
Sbjct: 335 GSSFYGLDITAIAVGGVKLPVSSSTFSTGGAIIDSGTVITRLPPTAYGALRSAFRQGMSK 394
Query: 1222 YPSAPALSILDTCYDLSGFKTVSIPKILLHFAGGTDLDIPPTGLLFAQSTSQACLPFAGN 1401
YPSA LSILDTCYDLSG+K SIP I FAGG + +PP G+LF ST Q CL FA N
Sbjct: 395 YPSAGELSILDTCYDLSGYKVFSIPTIEFSFAGGVTVKLPPQGILFVASTKQVCLAFAAN 454
Query: 1402 SDAGDVAIIGNKQQQTVEIVFDVAGGKLGFGPGGC 1506
D DV I GN QQ+T+E+V+DV GG++GFG GGC
Sbjct: 455 GDDSDVTIYGNVQQRTIEVVYDVGGGRIGFGAGGC 489