BLASTX nr result
ID: Coptis24_contig00002157
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00002157
(3292 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002280912.1| PREDICTED: uncharacterized protein LOC100252... 536 e-149
ref|XP_004167114.1| PREDICTED: uncharacterized LOC101207849 [Cuc... 516 e-143
ref|XP_004147482.1| PREDICTED: uncharacterized protein LOC101207... 516 e-143
ref|NP_178539.2| Cation efflux family protein [Arabidopsis thali... 484 e-134
dbj|BAM64839.1| hypothetical protein [Beta vulgaris] 437 e-119
>ref|XP_002280912.1| PREDICTED: uncharacterized protein LOC100252487 [Vitis vinifera]
Length = 807
Score = 536 bits (1380), Expect = e-149
Identities = 280/397 (70%), Positives = 313/397 (78%), Gaps = 9/397 (2%)
Frame = +2
Query: 545 PLFSIYSITQSQFHLIFGKSVLLAIVFLLRFQALRYCGTSATILAEISGNVAARFVTKNG 724
P+FS+ ITQSQ L+ KSVLLA++FLLRFQALRYCGT+ TILAE+SGNVAARFV +
Sbjct: 136 PIFSLTQITQSQHKLLVSKSVLLAVIFLLRFQALRYCGTATTILAELSGNVAARFVAEGR 195
Query: 725 NDGW-----SYASKVRGFCALFIGLFLLSISWDRIECFPLSG----KDGFVLFKGGNCLR 877
N + S KVRGF ALF+GLFLLS+SW RIECFPLS K GF LF NC+R
Sbjct: 196 NRNFGVRDRSGLPKVRGFFALFVGLFLLSMSWYRIECFPLSSMIVDKWGFSLFPRENCVR 255
Query: 878 VLPMLLPFMSGFLGCYERVSMNWGSIRQLGKKRVRLISLFFTTVVLFFPALISFFLFEED 1057
VLPMLLPF+SGFLGCYERVSMNWG+IRQLG+KRVRLISLFFTTV+LF PA++S +FE +
Sbjct: 256 VLPMLLPFLSGFLGCYERVSMNWGTIRQLGRKRVRLISLFFTTVILFVPAVVSILMFEAE 315
Query: 1058 GVVVSIGNLSWPLANTVVFGVLLSENYSDEKLISSKDFRREFGVIFICTLVLELFYFPEX 1237
G SI +L WPLANTV+FGVLL+ENYSDEKL+SS+DF+REF V F CTLVLELFYFPE
Sbjct: 316 GDEFSIASLGWPLANTVLFGVLLTENYSDEKLVSSRDFQREFLVTFFCTLVLELFYFPEL 375
Query: 1238 XXXXXXXXXXXXXIAVRELDPVSLKYPELGFDSPDSFSSMIMKPIRHILSERKSRKIALF 1417
I+VRELDPV ELG DS +SF+ I+KPIRH+LSERKSRKIALF
Sbjct: 376 SLWGLLLCGLLLYISVRELDPVYSNSLELGMDSSESFTDSIVKPIRHVLSERKSRKIALF 435
Query: 1418 LLINTAYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANGQFNYGRG 1597
LLINT YMVVEFV+GFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPAN QFNYGRG
Sbjct: 436 LLINTGYMVVEFVSGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANSQFNYGRG 495
Query: 1598 RFEVLSGYXXXXXXXXXXXXXXXESFERILDPQEIST 1708
RFEVLSGY ESFERIL+PQEIST
Sbjct: 496 RFEVLSGYVNAVFLVLVGALIVLESFERILEPQEIST 532
Score = 212 bits (540), Expect = 5e-52
Identities = 104/158 (65%), Positives = 122/158 (77%), Gaps = 1/158 (0%)
Frame = +2
Query: 2279 RSGDPKKK-HRHIDHNMEGIFLHVLADTMGSVGVVISTLLIRYKGWLIADPXXXXXXXXX 2455
R +P+K+ H+HIDHNMEGIFLHVLADTMGSVGVVISTLLI+YKGW +ADP
Sbjct: 648 RDQEPQKRNHKHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWQVADPACSIFISVL 707
Query: 2456 XXXXXXXLLRNSAEILLQRVPRAHEQELKQAMDDIMSIHGVHNIENVHVWSFTNTDVVGT 2635
LLRNSAEILLQRVPR HE +LK A+ ++M I GV I+N+HVWSFTNTDVVGT
Sbjct: 708 IVASVIPLLRNSAEILLQRVPRVHEHDLKDALKNVMKIRGVCGIQNLHVWSFTNTDVVGT 767
Query: 2636 VHLHVTATADKDSTKAQVSHILRDVGINDLTIQTEYIK 2749
+HLH++ ADK STK QVS+IL D GI DLT+Q EY+K
Sbjct: 768 LHLHISTEADKASTKVQVSNILHDAGIKDLTVQVEYVK 805
>ref|XP_004167114.1| PREDICTED: uncharacterized LOC101207849 [Cucumis sativus]
Length = 818
Score = 516 bits (1329), Expect = e-143
Identities = 264/397 (66%), Positives = 310/397 (78%), Gaps = 10/397 (2%)
Frame = +2
Query: 548 LFSIYSITQSQFHLIFGKSVLLAIVFLLRFQALRYCGTSATILAEISGNVAARFVTKN-- 721
+F + SI+Q Q + KS+LLAIVFLLRFQAL YCGT+A ILAE++GNVAARF+ +
Sbjct: 134 VFFLSSISQPQLKTMVVKSILLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLAERRN 193
Query: 722 ----GNDGWSYASKVRGFCALFIGLFLLSISWDRIECFPLS----GKDGFVLFKGGNCLR 877
G+ S +S+VRGF +LF+GLFLLSISWDRI+CFP + K GF + NC+R
Sbjct: 194 RMTMGDRTRSRSSEVRGFLSLFLGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMR 253
Query: 878 VLPMLLPFMSGFLGCYERVSMNWGSIRQLGKKRVRLISLFFTTVVLFFPALISFFLFEED 1057
+ PMLLPF+SGFLGCYER+SMNWGS++QLG+KRVRL+SLFFTT++LF PA+IS LFE +
Sbjct: 254 IWPMLLPFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAE 313
Query: 1058 GVVVSIGNLSWPLANTVVFGVLLSENYSDEKLISSKDFRREFGVIFICTLVLELFYFPEX 1237
G VS GNL+WPLANTVVFGVLL+ENYSD+KL+SSKDFR EF V F+CT++LEL YF E
Sbjct: 314 GKSVSFGNLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFSEL 373
Query: 1238 XXXXXXXXXXXXXIAVRELDPVSLKYPELGFDSPDSFSSMIMKPIRHILSERKSRKIALF 1417
+AVRELDPV L Y ELG +S DS +M+M+P+RHIL+ERKSRKIALF
Sbjct: 374 SLWGLLFCGLLLYVAVRELDPVYLNYLELGVESSDSIVTMVMRPVRHILTERKSRKIALF 433
Query: 1418 LLINTAYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANGQFNYGRG 1597
LL+NT YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPAN QFNYGRG
Sbjct: 434 LLLNTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRG 493
Query: 1598 RFEVLSGYXXXXXXXXXXXXXXXESFERILDPQEIST 1708
RFE+LSGY ES ERILDPQEIST
Sbjct: 494 RFEILSGYANAVFLVLVGALIVLESLERILDPQEIST 530
Score = 214 bits (544), Expect = 2e-52
Identities = 109/166 (65%), Positives = 124/166 (74%)
Frame = +2
Query: 2255 KEPLLEKVRSGDPKKKHRHIDHNMEGIFLHVLADTMGSVGVVISTLLIRYKGWLIADPXX 2434
K+P+ VR K H HIDHNMEGIFLHVLADTMGSVGVVISTLLI+YKGWL+ADP
Sbjct: 654 KQPVEGTVRK---KHHHHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPAC 710
Query: 2435 XXXXXXXXXXXXXXLLRNSAEILLQRVPRAHEQELKQAMDDIMSIHGVHNIENVHVWSFT 2614
LLRNSAEILLQRVPRAHEQ+LK+A+ DIM IHGV I+N+HVWSFT
Sbjct: 711 SIFISIMIISSVIPLLRNSAEILLQRVPRAHEQDLKEAVRDIMEIHGVQGIQNLHVWSFT 770
Query: 2615 NTDVVGTVHLHVTATADKDSTKAQVSHILRDVGINDLTIQTEYIKE 2752
NTDVVGT+ LHV+ DK S KA+V HIL D GI DLT+Q E+ +E
Sbjct: 771 NTDVVGTLRLHVSTETDKPSIKAKVEHILHDAGIKDLTLQLEHNQE 816
>ref|XP_004147482.1| PREDICTED: uncharacterized protein LOC101207849 [Cucumis sativus]
Length = 820
Score = 516 bits (1329), Expect = e-143
Identities = 264/397 (66%), Positives = 310/397 (78%), Gaps = 10/397 (2%)
Frame = +2
Query: 548 LFSIYSITQSQFHLIFGKSVLLAIVFLLRFQALRYCGTSATILAEISGNVAARFVTKN-- 721
+F + SI+Q Q + KS+LLAIVFLLRFQAL YCGT+A ILAE++GNVAARF+ +
Sbjct: 134 VFFLSSISQPQLKTMVVKSILLAIVFLLRFQALLYCGTAAMILAEMTGNVAARFLAERRN 193
Query: 722 ----GNDGWSYASKVRGFCALFIGLFLLSISWDRIECFPLS----GKDGFVLFKGGNCLR 877
G+ S +S+VRGF +LF+GLFLLSISWDRI+CFP + K GF + NC+R
Sbjct: 194 RMTMGDRTRSRSSEVRGFLSLFLGLFLLSISWDRIDCFPFATSFIDKYGFSVLPRENCMR 253
Query: 878 VLPMLLPFMSGFLGCYERVSMNWGSIRQLGKKRVRLISLFFTTVVLFFPALISFFLFEED 1057
+ PMLLPF+SGFLGCYER+SMNWGS++QLG+KRVRL+SLFFTT++LF PA+IS LFE +
Sbjct: 254 IWPMLLPFLSGFLGCYERISMNWGSVKQLGQKRVRLVSLFFTTIILFVPAVISMLLFEAE 313
Query: 1058 GVVVSIGNLSWPLANTVVFGVLLSENYSDEKLISSKDFRREFGVIFICTLVLELFYFPEX 1237
G VS GNL+WPLANTVVFGVLL+ENYSD+KL+SSKDFR EF V F+CT++LEL YF E
Sbjct: 314 GKSVSFGNLAWPLANTVVFGVLLNENYSDDKLVSSKDFRSEFLVTFVCTVILELLYFSEL 373
Query: 1238 XXXXXXXXXXXXXIAVRELDPVSLKYPELGFDSPDSFSSMIMKPIRHILSERKSRKIALF 1417
+AVRELDPV L Y ELG +S DS +M+M+P+RHIL+ERKSRKIALF
Sbjct: 374 SLWGLLFCGLLLYVAVRELDPVYLNYLELGVESSDSIVTMVMRPVRHILTERKSRKIALF 433
Query: 1418 LLINTAYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANGQFNYGRG 1597
LL+NT YMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPAN QFNYGRG
Sbjct: 434 LLLNTGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANNQFNYGRG 493
Query: 1598 RFEVLSGYXXXXXXXXXXXXXXXESFERILDPQEIST 1708
RFE+LSGY ES ERILDPQEIST
Sbjct: 494 RFEILSGYANAVFLVLVGALIVLESLERILDPQEIST 530
Score = 216 bits (550), Expect = 3e-53
Identities = 113/193 (58%), Positives = 129/193 (66%)
Frame = +2
Query: 2174 HESHSNGEFHTHNAXXXXXXXXXXXXXKEPLLEKVRSGDPKKKHRHIDHNMEGIFLHVLA 2353
H+ E H H K+P+ VR K H HIDHNMEGIFLHVLA
Sbjct: 629 HQHDCKHEHHDHVHQHDQHENSHSHPSKQPVEGTVRK---KHHHHHIDHNMEGIFLHVLA 685
Query: 2354 DTMGSVGVVISTLLIRYKGWLIADPXXXXXXXXXXXXXXXXLLRNSAEILLQRVPRAHEQ 2533
DTMGSVGVVISTLLI+YKGWL+ADP LLRNSAEILLQRVPRAHEQ
Sbjct: 686 DTMGSVGVVISTLLIKYKGWLVADPACSIFISIMIISSVIPLLRNSAEILLQRVPRAHEQ 745
Query: 2534 ELKQAMDDIMSIHGVHNIENVHVWSFTNTDVVGTVHLHVTATADKDSTKAQVSHILRDVG 2713
+LK+A+ DIM IHGV I+N+HVWSFTNTDVVGT+ LHV+ DK S KA+V HIL D G
Sbjct: 746 DLKEAVRDIMEIHGVQGIQNLHVWSFTNTDVVGTLRLHVSTETDKPSIKAKVEHILHDAG 805
Query: 2714 INDLTIQTEYIKE 2752
I DLT+Q E+ +E
Sbjct: 806 IKDLTLQLEHNQE 818
>ref|NP_178539.2| Cation efflux family protein [Arabidopsis thaliana]
gi|330250755|gb|AEC05849.1| Cation efflux family protein
[Arabidopsis thaliana]
Length = 798
Score = 484 bits (1246), Expect = e-134
Identities = 257/390 (65%), Positives = 291/390 (74%), Gaps = 5/390 (1%)
Frame = +2
Query: 554 SIYSITQSQFHLIFGKSVLLAIVFLLRFQALRYCGTSATILAEISGNVAARFVTKNGNDG 733
SI S++ SQ L+ KS LLA VFLLRFQALRYCG +A ILAE+SG V+AR + +
Sbjct: 131 SISSLSSSQIKLLLAKSFLLAFVFLLRFQALRYCGAAAMILAELSGTVSARVLFSDTGGI 190
Query: 734 WSYASKVRGFCALFIGLFLLSISWDRIECFPLSGKD---GFVLFKGGNCLRVLPMLLPFM 904
+SKVRGFC LF GL LLSISWDR++CFP S GF ++ NCLR+ P+LLPF+
Sbjct: 191 GVRSSKVRGFCVLFAGLLLLSISWDRVDCFPFSSSVESWGFWIYPKENCLRIWPLLLPFL 250
Query: 905 SGFLGCYERVSMNWGSIRQLGKKRVRLISLFFTTVVLFFPALISFFLFEEDGVVVSIGNL 1084
SGFLGCYE+VS+NW I+QL +KRVRL+SLF TTV+LF A+ SFF VS GNL
Sbjct: 251 SGFLGCYEKVSVNWNEIKQLDQKRVRLLSLFLTTVLLFPLAIWSFFFSGSGDDSVSFGNL 310
Query: 1085 SWPLANTVVFGVLLSENYSDEKLISSK--DFRREFGVIFICTLVLELFYFPEXXXXXXXX 1258
WPLANTVVFGVLLSENY+D+K SSK D REF V F+CT+VLELFYFPE
Sbjct: 311 GWPLANTVVFGVLLSENYNDDKFSSSKKKDSEREFLVTFLCTIVLELFYFPELSLWGLLL 370
Query: 1259 XXXXXXIAVRELDPVSLKYPELGFDSPDSFSSMIMKPIRHILSERKSRKIALFLLINTAY 1438
IAVREL+ V Y E+G +SP+SFS+M MKPIRHILSE+KSRKIALFLLINTAY
Sbjct: 371 CGLLLYIAVRELESVYSDYQEIGMESPESFSTMFMKPIRHILSEKKSRKIALFLLINTAY 430
Query: 1439 MVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANGQFNYGRGRFEVLSG 1618
MVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPAN Q+NYGRGRFEVLSG
Sbjct: 431 MVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYISRLPANHQYNYGRGRFEVLSG 490
Query: 1619 YXXXXXXXXXXXXXXXESFERILDPQEIST 1708
Y ES ERILDPQEIST
Sbjct: 491 YVNAVFLVLVGALIVLESIERILDPQEIST 520
Score = 196 bits (499), Expect = 3e-47
Identities = 97/151 (64%), Positives = 115/151 (76%)
Frame = +2
Query: 2294 KKKHRHIDHNMEGIFLHVLADTMGSVGVVISTLLIRYKGWLIADPXXXXXXXXXXXXXXX 2473
KK+HRHIDHNMEGIFLHVLADTMGSVGVVISTLLI+YKGWL+ADP
Sbjct: 646 KKEHRHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKGWLVADPASSIFISILIIASVI 705
Query: 2474 XLLRNSAEILLQRVPRAHEQELKQAMDDIMSIHGVHNIENVHVWSFTNTDVVGTVHLHVT 2653
LLRNSAEILLQRVPRAH Q+LK+AM +I+ GV +I+ +HVWSFTN+DVV T+HL V+
Sbjct: 706 PLLRNSAEILLQRVPRAHRQDLKEAMRNILKTKGVCSIQRLHVWSFTNSDVVATLHLLVS 765
Query: 2654 ATADKDSTKAQVSHILRDVGINDLTIQTEYI 2746
A +DK TK QVS +L D G+ D T+Q E +
Sbjct: 766 ADSDKTDTKLQVSRLLEDAGVKDWTLQVESV 796
>dbj|BAM64839.1| hypothetical protein [Beta vulgaris]
Length = 931
Score = 437 bits (1123), Expect = e-119
Identities = 224/361 (62%), Positives = 272/361 (75%), Gaps = 6/361 (1%)
Frame = +2
Query: 644 LRYCGTSATILAEISGNVAARFVTKNGNDGWSYASKVRGFCALFIGLFLLSISWDRIECF 823
LRYCGT+A ILAE+SGN+A RF D + S+VRGF ALF+GLFLLS+SWDR++CF
Sbjct: 38 LRYCGTAAMILAELSGNIAFRFWKDQDWDRDGH-SRVRGFFALFVGLFLLSVSWDRMDCF 96
Query: 824 PLSGKD------GFVLFKGGNCLRVLPMLLPFMSGFLGCYERVSMNWGSIRQLGKKRVRL 985
PLS + ++ CLR++PMLLPF++GFLGC ER MNWG+IRQLG+KRV+L
Sbjct: 97 PLSHSNVDKIQSSLMVVVDMKCLRIVPMLLPFLAGFLGCCERDMMNWGTIRQLGRKRVQL 156
Query: 986 ISLFFTTVVLFFPALISFFLFEEDGVVVSIGNLSWPLANTVVFGVLLSENYSDEKLISSK 1165
+SLFFTT +L FPA+++ +FE +G VSI L W LANTV+FGVLL+E Y+DEKL++ +
Sbjct: 157 VSLFFTTFMLLFPAIVNMLVFEAEGGSVSIMTLGWLLANTVLFGVLLNEWYNDEKLVNPR 216
Query: 1166 DFRREFGVIFICTLVLELFYFPEXXXXXXXXXXXXXXIAVRELDPVSLKYPELGFDSPDS 1345
D +EF + F CTLVLEL YFPE +A+R+L+ ++ E+G +S ++
Sbjct: 217 DSEKEFLITFACTLVLELVYFPELSLWGLLICGFLLWVAIRQLNWAHARFVEIGAESSET 276
Query: 1346 FSSMIMKPIRHILSERKSRKIALFLLINTAYMVVEFVAGFMSNSLGLISDACHMLFDCAA 1525
FSSM+M+PIRH+LSERKSRKIALFL+INT YMVVEFVAGFMSNSLGLISDACHMLFDCAA
Sbjct: 277 FSSMVMRPIRHVLSERKSRKIALFLMINTGYMVVEFVAGFMSNSLGLISDACHMLFDCAA 336
Query: 1526 LAIGLYASYISRLPANGQFNYGRGRFEVLSGYXXXXXXXXXXXXXXXESFERILDPQEIS 1705
LAIGLYASYISRLPAN QFNYGRGRFEVLSGY ES ERILDPQEIS
Sbjct: 337 LAIGLYASYISRLPANEQFNYGRGRFEVLSGYVNAVFLVLVGALIVLESLERILDPQEIS 396
Query: 1706 T 1708
T
Sbjct: 397 T 397
Score = 198 bits (504), Expect = 7e-48
Identities = 96/152 (63%), Positives = 115/152 (75%)
Frame = +2
Query: 2288 DPKKKHRHIDHNMEGIFLHVLADTMGSVGVVISTLLIRYKGWLIADPXXXXXXXXXXXXX 2467
D K +H+HIDHNMEGIFLHVLADT+GSVGVVISTLLI YKGWL+ADP
Sbjct: 612 DKKHRHQHIDHNMEGIFLHVLADTLGSVGVVISTLLINYKGWLVADPACSIFISVLIVSS 671
Query: 2468 XXXLLRNSAEILLQRVPRAHEQELKQAMDDIMSIHGVHNIENVHVWSFTNTDVVGTVHLH 2647
LLRNSA ILLQRVPRAHEQ+LK A++D+M I GV I N+HVWS TNTDV+GT+ L
Sbjct: 672 VIPLLRNSAAILLQRVPRAHEQDLKAAVNDVMKIEGVSGIRNLHVWSLTNTDVIGTLKLR 731
Query: 2648 VTATADKDSTKAQVSHILRDVGINDLTIQTEY 2743
V++ DK +TKA+V HI +D G+ DLT+Q E+
Sbjct: 732 VSSDIDKAATKARVMHIFQDAGVKDLTLQIEW 763