BLASTX nr result
ID: Coptis24_contig00002156
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00002156
(1384 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002284873.1| PREDICTED: tRNA-splicing endonuclease subuni... 263 6e-68
ref|XP_002313727.1| predicted protein [Populus trichocarpa] gi|2... 256 1e-65
gb|ABK93461.1| unknown [Populus trichocarpa] 254 3e-65
ref|XP_002526336.1| tRNA-splicing endonuclease subunit Sen2-2, p... 253 6e-65
ref|NP_974966.1| tRNA-splicing endonuclease subunit Sen2-2 [Arab... 237 5e-60
>ref|XP_002284873.1| PREDICTED: tRNA-splicing endonuclease subunit Sen2-1 [Vitis vinifera]
Length = 242
Score = 263 bits (673), Expect = 6e-68
Identities = 138/243 (56%), Positives = 174/243 (71%), Gaps = 4/243 (1%)
Frame = -2
Query: 1314 GTRWKGKGSEAFALSNPMSKIVSRLQNSLTESKASGLLMDRVVDCDRYCAVLLEVELEQA 1135
G RWKGKG+EA AL+ PMS+IVS+LQ+SLT S + GLL C+VLLE EQ
Sbjct: 2 GPRWKGKGAEAKALAEPMSEIVSQLQSSLTRSDSQGLLSG--------CSVLLEAGAEQT 53
Query: 1134 NLLDQACFGCHIVTACKDKKHYELSLEEAFYLCHSLKCLEVVWRDKCLENDE-LWEFMTS 958
LL+++CFG ++TA KDK+ ++LS+EEAFYLCHSL CL++V D L+NDE LW++M S
Sbjct: 54 ELLNRSCFGRPVITAEKDKQWFQLSMEEAFYLCHSLGCLKIVGEDNHLKNDEELWQYMKS 113
Query: 957 RRKLFPFFYKAYSHLRAKNWVVKSEIKYGVDFVAYHHHPSLVHSEYAVIVL-KGNEDIT- 784
RR+ FP FY AYSHLR KNWVV+S +YGVDFVAY HHP+LVHSEYAV+VL + NE+
Sbjct: 114 RRETFPDFYMAYSHLRIKNWVVRSGSQYGVDFVAYRHHPALVHSEYAVLVLSEENENANG 173
Query: 783 RLMAWPSFHAILRLEDGVAKTLLLLHIN-NVHDRVSSSCFEHYTVEEQIIARWNPEQCHE 607
RL W F +RL VAKTLL++HIN N H + C + Y+VEE+ I RW+ EQC E
Sbjct: 174 RLRVWSDFDCTIRLCGSVAKTLLVIHINKNGHGATTPLCLKKYSVEERTITRWSAEQCRE 233
Query: 606 EHT 598
T
Sbjct: 234 VQT 236
Score = 170 bits (430), Expect = 9e-40
Identities = 82/155 (52%), Positives = 105/155 (67%)
Frame = -2
Query: 465 LNTRWKGKGSKAIALANPMSKIVAXXXXXXXXSKACXXXXXXXXXXXXXXXXADLLNRAC 286
+ RWKGKG++A ALA PMS+IV+ S + +LLNR+C
Sbjct: 1 MGPRWKGKGAEAKALAEPMSEIVSQLQSSLTRSDSQGLLSGCSVLLEAGAEQTELLNRSC 60
Query: 285 FGHPIITDNEDSQWYELSLEEAFYLCHFLKCLDIVGKNHCFQNIEKVWQFMKSKRALFPI 106
FG P+IT +D QW++LS+EEAFYLCH L CL IVG+++ +N E++WQ+MKS+R FP
Sbjct: 61 FGRPVITAEKDKQWFQLSMEEAFYLCHSLGCLKIVGEDNHLKNDEELWQYMKSRRETFPD 120
Query: 105 SYKAYSHLRAKNWFVRSGIQYGVNFVAYSHHPSLV 1
Y AYSHLR KNW VRSG QYGV+FVAY HHP+LV
Sbjct: 121 FYMAYSHLRIKNWVVRSGSQYGVDFVAYRHHPALV 155
>ref|XP_002313727.1| predicted protein [Populus trichocarpa] gi|222850135|gb|EEE87682.1|
predicted protein [Populus trichocarpa]
Length = 235
Score = 256 bits (654), Expect = 1e-65
Identities = 133/243 (54%), Positives = 171/243 (70%), Gaps = 2/243 (0%)
Frame = -2
Query: 1314 GTRWKGKGSEAFALSNPMSKIVSRLQNSLTESKASGLLMDRVVDCDRYCAVLLEVELEQA 1135
G RWKGKG+EA AL++PMS+IVS+LQ+SL +S A L C+VLL VE EQ
Sbjct: 2 GPRWKGKGAEAKALADPMSEIVSQLQSSLLQSNACASLSG--------CSVLLAVETEQT 53
Query: 1134 NLLDQACFGCHIVTACKDKKHYELSLEEAFYLCHSLKCLEVVWRDKCLEND-ELWEFMTS 958
LL +ACFG I+TA K+K+ ++L LEEAFYLC+SLKCL++ D ++ND +LW +M
Sbjct: 54 ELLTRACFGKPIITAEKEKQWFQLGLEEAFYLCYSLKCLKIAGEDNFVKNDLDLWLYMKL 113
Query: 957 RRKLFPFFYKAYSHLRAKNWVVKSEIKYGVDFVAYHHHPSLVHSEYAVIVLKGNEDITRL 778
+++ FP FYKAYSHLR KNWV++ ++YGVDFVAY HHPSLVHSEYAVIVL D RL
Sbjct: 114 KKEKFPDFYKAYSHLRMKNWVLRPGLQYGVDFVAYQHHPSLVHSEYAVIVL-SEGDTGRL 172
Query: 777 MAWPSFHAILRLEDGVAKTLLLLHIN-NVHDRVSSSCFEHYTVEEQIIARWNPEQCHEEH 601
W FH +RL VAKTLL+L+++ N H +S SC E Y+VEE I RW+PEQ ++
Sbjct: 173 RVWSDFHCTIRLCGSVAKTLLILNVDKNGHGAISPSCLERYSVEECTITRWSPEQSRDDK 232
Query: 600 TTS 592
S
Sbjct: 233 KAS 235
Score = 165 bits (417), Expect = 3e-38
Identities = 80/155 (51%), Positives = 102/155 (65%)
Frame = -2
Query: 465 LNTRWKGKGSKAIALANPMSKIVAXXXXXXXXSKACXXXXXXXXXXXXXXXXADLLNRAC 286
+ RWKGKG++A ALA+PMS+IV+ S AC +LL RAC
Sbjct: 1 MGPRWKGKGAEAKALADPMSEIVSQLQSSLLQSNACASLSGCSVLLAVETEQTELLTRAC 60
Query: 285 FGHPIITDNEDSQWYELSLEEAFYLCHFLKCLDIVGKNHCFQNIEKVWQFMKSKRALFPI 106
FG PIIT ++ QW++L LEEAFYLC+ LKCL I G+++ +N +W +MK K+ FP
Sbjct: 61 FGKPIITAEKEKQWFQLGLEEAFYLCYSLKCLKIAGEDNFVKNDLDLWLYMKLKKEKFPD 120
Query: 105 SYKAYSHLRAKNWFVRSGIQYGVNFVAYSHHPSLV 1
YKAYSHLR KNW +R G+QYGV+FVAY HHPSLV
Sbjct: 121 FYKAYSHLRMKNWVLRPGLQYGVDFVAYQHHPSLV 155
>gb|ABK93461.1| unknown [Populus trichocarpa]
Length = 235
Score = 254 bits (650), Expect = 3e-65
Identities = 132/243 (54%), Positives = 171/243 (70%), Gaps = 2/243 (0%)
Frame = -2
Query: 1314 GTRWKGKGSEAFALSNPMSKIVSRLQNSLTESKASGLLMDRVVDCDRYCAVLLEVELEQA 1135
G RWKGKG+EA AL++PMS+IVS+LQ+SL +S A L C+VLL VE E+
Sbjct: 2 GPRWKGKGAEAKALADPMSEIVSQLQSSLLQSNACASLSG--------CSVLLAVETEKT 53
Query: 1134 NLLDQACFGCHIVTACKDKKHYELSLEEAFYLCHSLKCLEVVWRDKCLEND-ELWEFMTS 958
LL +ACFG I+TA K+K+ ++L LEEAFYLC+SLKCL++ D ++ND +LW +M
Sbjct: 54 ELLTRACFGKPIITAEKEKQWFQLGLEEAFYLCYSLKCLKIAGEDNFVKNDLDLWLYMKL 113
Query: 957 RRKLFPFFYKAYSHLRAKNWVVKSEIKYGVDFVAYHHHPSLVHSEYAVIVLKGNEDITRL 778
+++ FP FYKAYSHLR KNWV++ ++YGVDFVAY HHPSLVHSEYAVIVL D RL
Sbjct: 114 KKEKFPDFYKAYSHLRMKNWVLRPGLQYGVDFVAYQHHPSLVHSEYAVIVL-SEGDTGRL 172
Query: 777 MAWPSFHAILRLEDGVAKTLLLLHIN-NVHDRVSSSCFEHYTVEEQIIARWNPEQCHEEH 601
W FH +RL VAKTLL+L+++ N H +S SC E Y+VEE I RW+PEQ ++
Sbjct: 173 RVWSDFHCTIRLCGSVAKTLLILNVDKNGHGAISPSCLERYSVEECTITRWSPEQSRDDK 232
Query: 600 TTS 592
S
Sbjct: 233 KAS 235
Score = 165 bits (417), Expect = 3e-38
Identities = 80/155 (51%), Positives = 102/155 (65%)
Frame = -2
Query: 465 LNTRWKGKGSKAIALANPMSKIVAXXXXXXXXSKACXXXXXXXXXXXXXXXXADLLNRAC 286
+ RWKGKG++A ALA+PMS+IV+ S AC +LL RAC
Sbjct: 1 MGPRWKGKGAEAKALADPMSEIVSQLQSSLLQSNACASLSGCSVLLAVETEKTELLTRAC 60
Query: 285 FGHPIITDNEDSQWYELSLEEAFYLCHFLKCLDIVGKNHCFQNIEKVWQFMKSKRALFPI 106
FG PIIT ++ QW++L LEEAFYLC+ LKCL I G+++ +N +W +MK K+ FP
Sbjct: 61 FGKPIITAEKEKQWFQLGLEEAFYLCYSLKCLKIAGEDNFVKNDLDLWLYMKLKKEKFPD 120
Query: 105 SYKAYSHLRAKNWFVRSGIQYGVNFVAYSHHPSLV 1
YKAYSHLR KNW +R G+QYGV+FVAY HHPSLV
Sbjct: 121 FYKAYSHLRMKNWVLRPGLQYGVDFVAYQHHPSLV 155
>ref|XP_002526336.1| tRNA-splicing endonuclease subunit Sen2-2, putative [Ricinus
communis] gi|223534295|gb|EEF36007.1| tRNA-splicing
endonuclease subunit Sen2-2, putative [Ricinus communis]
Length = 237
Score = 253 bits (647), Expect = 6e-65
Identities = 136/241 (56%), Positives = 167/241 (69%), Gaps = 5/241 (2%)
Frame = -2
Query: 1314 GTRWKGKGSEAFALSNPMSKIVSRLQNSLTESKASGLLMDRVVDCDRYCAVLLEVELEQA 1135
GTRWKGKGSEA A ++PMSKI+S+L +SL + A GLL C+VLL VE EQ
Sbjct: 2 GTRWKGKGSEAKAAADPMSKIISQLCSSLIQFDAHGLLSG--------CSVLLAVESEQT 53
Query: 1134 NLLDQACFGCHIVTACKDKKHYELSLEEAFYLCHSLKCLEVVWRDKCLEND-ELWEFMTS 958
LL +ACFG ++T+ KDK+ ++L +EEAFYL +SLKC+++V D C +D ELW+FM S
Sbjct: 54 ELLTRACFGRPMITSEKDKQWFQLGIEEAFYLSYSLKCIKIVSEDNCCISDLELWQFMKS 113
Query: 957 RRKLFPFFYKAYSHLRAKNWVVKSEIKYGVDFVAYHHHPSLVHSEYAVIVLKGNEDIT-- 784
++ FP FYKAYSHLR KNWVV+ +YGVDFVAY HHPSLVHSEYAV+VL ED
Sbjct: 114 QKPTFPDFYKAYSHLRMKNWVVRPGSQYGVDFVAYRHHPSLVHSEYAVLVL-SEEDANAN 172
Query: 783 -RLMAWPSFHAILRLEDGVAKTLLLLHIN-NVHDRVSSSCFEHYTVEEQIIARWNPEQCH 610
RL W FH LRL VAKTLL+L IN N H SC E Y+VEE+ ARW+P+Q
Sbjct: 173 GRLRVWSDFHCTLRLCGSVAKTLLVLGINKNCHGTTLPSCLEKYSVEERTFARWSPQQSR 232
Query: 609 E 607
E
Sbjct: 233 E 233
Score = 163 bits (413), Expect = 9e-38
Identities = 79/155 (50%), Positives = 101/155 (65%)
Frame = -2
Query: 465 LNTRWKGKGSKAIALANPMSKIVAXXXXXXXXSKACXXXXXXXXXXXXXXXXADLLNRAC 286
+ TRWKGKGS+A A A+PMSKI++ A +LL RAC
Sbjct: 1 MGTRWKGKGSEAKAAADPMSKIISQLCSSLIQFDAHGLLSGCSVLLAVESEQTELLTRAC 60
Query: 285 FGHPIITDNEDSQWYELSLEEAFYLCHFLKCLDIVGKNHCFQNIEKVWQFMKSKRALFPI 106
FG P+IT +D QW++L +EEAFYL + LKC+ IV +++C + ++WQFMKS++ FP
Sbjct: 61 FGRPMITSEKDKQWFQLGIEEAFYLSYSLKCIKIVSEDNCCISDLELWQFMKSQKPTFPD 120
Query: 105 SYKAYSHLRAKNWFVRSGIQYGVNFVAYSHHPSLV 1
YKAYSHLR KNW VR G QYGV+FVAY HHPSLV
Sbjct: 121 FYKAYSHLRMKNWVVRPGSQYGVDFVAYRHHPSLV 155
>ref|NP_974966.1| tRNA-splicing endonuclease subunit Sen2-2 [Arabidopsis thaliana]
gi|332009914|gb|AED97297.1| tRNA-splicing endonuclease
subunit Sen2-2 [Arabidopsis thaliana]
Length = 255
Score = 237 bits (605), Expect = 5e-60
Identities = 125/243 (51%), Positives = 167/243 (68%), Gaps = 4/243 (1%)
Frame = -2
Query: 1308 RWKGKGSEAFALSNPMSKIVSRLQNSLTESKASGLLMDRVVDCDRYCAVLLEVELEQANL 1129
RWK KG+EA AL+ P+SK VS LQ SL E+++SG L C VLL VE EQA L
Sbjct: 9 RWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSS--------CNVLLAVEPEQAEL 60
Query: 1128 LDQACFGCHIVTACKDKKHYELSLEEAFYLCHSLKCLEVVWRDKCLEND-ELWEFMTSRR 952
LD+ CFG +++A K KK +LS EEAF+L ++LKC+++ + +CLEN+ + W +M S+R
Sbjct: 61 LDRCCFGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKR 120
Query: 951 KLFPFFYKAYSHLRAKNWVVKSEIKYGVDFVAYHHHPSLVHSEYAVIVLKGNEDITRLMA 772
FP F+KAYSHLR+KNWV++S ++YGVDFVAY HHPSLVHSEY+V+V G+ D RL
Sbjct: 121 PNFPMFFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLVQSGDSD--RLRV 178
Query: 771 WPSFHAILRLEDGVAKTLLLLHIN---NVHDRVSSSCFEHYTVEEQIIARWNPEQCHEEH 601
W H +RL VAKTLL L++N D C E++TVEEQ I+RW+PE E+
Sbjct: 179 WSDIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSREDQ 238
Query: 600 TTS 592
+T+
Sbjct: 239 STN 241
Score = 139 bits (351), Expect = 1e-30
Identities = 69/152 (45%), Positives = 96/152 (63%)
Frame = -2
Query: 456 RWKGKGSKAIALANPMSKIVAXXXXXXXXSKACXXXXXXXXXXXXXXXXADLLNRACFGH 277
RWK KG++A ALA P+SK V+ +++ A+LL+R CFG
Sbjct: 9 RWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCCFGR 68
Query: 276 PIITDNEDSQWYELSLEEAFYLCHFLKCLDIVGKNHCFQNIEKVWQFMKSKRALFPISYK 97
+++ + +W +LS EEAF+L + LKC+ I + C +N W +MKSKR FP+ +K
Sbjct: 69 LVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPMFFK 128
Query: 96 AYSHLRAKNWFVRSGIQYGVNFVAYSHHPSLV 1
AYSHLR+KNW +RSG+QYGV+FVAY HHPSLV
Sbjct: 129 AYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLV 160