BLASTX nr result
ID: Coptis24_contig00002009
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00002009
(2125 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI15435.3| unnamed protein product [Vitis vinifera] 360 e-101
ref|XP_002275826.1| PREDICTED: cytochrome P450 71D10 [Vitis vini... 360 e-101
ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinif... 359 e-101
ref|XP_003634278.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P... 355 e-100
ref|XP_002511968.1| flavonoid 3-hydroxylase, putative [Ricinus c... 340 1e-95
>emb|CBI15435.3| unnamed protein product [Vitis vinifera]
Length = 439
Score = 360 bits (924), Expect(2) = e-101
Identities = 169/284 (59%), Positives = 222/284 (78%), Gaps = 4/284 (1%)
Frame = -2
Query: 1041 RRMKAVNMVLDGFLEKIIDEHVQDAKELHGTHRDFIDVMLSLIESNNTWELH----LGRD 874
RRMKA++ +D LEK+IDEH QDA E G H+DF+DVMLSL+ + + ++
Sbjct: 86 RRMKAISGAVDHILEKVIDEHKQDASENQGNHKDFVDVMLSLMNEMKNFHQEPSYLIEQE 145
Query: 873 HIKAIMVDMLAGTMDTSSTAIEWVIAELLKNPRAMKLVQEELQIVVGLDRIVEEADLIKL 694
+IK I+ D++ G +DTS+T IEW+++EL ++PR M+ +QEEL+ V+G++R+VEE DL L
Sbjct: 146 NIKGIVWDIIIGAIDTSATTIEWLLSELFRHPRVMRQLQEELENVIGMERMVEEVDLANL 205
Query: 693 DYLKMVIKESMRLHPVAPLLVPHESIEDITIKGYFIPKSSRVLINTWAIGRDPNVWSSNA 514
YL MV+KE +RLHP PLL+PHESIEDIT+ GY+IPK SR++IN WAIGRDPN+WS+N
Sbjct: 206 VYLDMVLKEGLRLHPAGPLLLPHESIEDITLNGYYIPKKSRIIINAWAIGRDPNIWSNNV 265
Query: 513 EEFFPERFIGTNIDFQGRDFRFLPFGSGRRKCPGMQLGSTVVELVLAQLVHCFNLELPDG 334
E+FFPERFIG+NIDFQG+DF+F+PFGSGRRKCPGMQLG V LVLAQLVHCF+ +LP+G
Sbjct: 266 EDFFPERFIGSNIDFQGKDFQFIPFGSGRRKCPGMQLGLINVRLVLAQLVHCFDWKLPNG 325
Query: 333 MSPNDLEMTEKFGLTLPRANHLFAVPTYRLRVNFGA*GSPVWTM 202
M P++L+M+E+FGL LPRA HL A+PTYRL P WT+
Sbjct: 326 MLPSELDMSEEFGLALPRATHLHALPTYRL--------LPKWTV 361
Score = 38.5 bits (88), Expect(2) = e-101
Identities = 16/31 (51%), Positives = 23/31 (74%)
Frame = -1
Query: 1234 LQQGVSIAGAFNLADFFPYLRAFDLQGFGRR 1142
+Q+ + + GAFN+AD+ P+L A DLQG RR
Sbjct: 57 VQETLILVGAFNIADYVPFLGALDLQGLKRR 87
>ref|XP_002275826.1| PREDICTED: cytochrome P450 71D10 [Vitis vinifera]
Length = 506
Score = 360 bits (923), Expect(2) = e-101
Identities = 166/270 (61%), Positives = 218/270 (80%), Gaps = 4/270 (1%)
Frame = -2
Query: 1041 RRMKAVNMVLDGFLEKIIDEHVQDAKELHGTHRDFIDVMLSLIESNNTWELH----LGRD 874
RRMKA++ +D LEK+IDEH QDA E G H+DF+DVMLSL+ + + ++
Sbjct: 233 RRMKAISGAVDHILEKVIDEHKQDASENQGNHKDFVDVMLSLMNEMKNFHQEPSYLIEQE 292
Query: 873 HIKAIMVDMLAGTMDTSSTAIEWVIAELLKNPRAMKLVQEELQIVVGLDRIVEEADLIKL 694
+IK I+ D++ G +DTS+T IEW+++EL ++PR M+ +QEEL+ V+G++R+VEE DL L
Sbjct: 293 NIKGIVWDIIIGAIDTSATTIEWLLSELFRHPRVMRQLQEELENVIGMERMVEEVDLANL 352
Query: 693 DYLKMVIKESMRLHPVAPLLVPHESIEDITIKGYFIPKSSRVLINTWAIGRDPNVWSSNA 514
YL MV+KE +RLHP PLL+PHESIEDIT+ GY+IPK SR++IN WAIGRDPN+WS+N
Sbjct: 353 VYLDMVLKEGLRLHPAGPLLLPHESIEDITLNGYYIPKKSRIIINAWAIGRDPNIWSNNV 412
Query: 513 EEFFPERFIGTNIDFQGRDFRFLPFGSGRRKCPGMQLGSTVVELVLAQLVHCFNLELPDG 334
E+FFPERFIG+NIDFQG+DF+F+PFGSGRRKCPGMQLG V LVLAQLVHCF+ +LP+G
Sbjct: 413 EDFFPERFIGSNIDFQGKDFQFIPFGSGRRKCPGMQLGLINVRLVLAQLVHCFDWKLPNG 472
Query: 333 MSPNDLEMTEKFGLTLPRANHLFAVPTYRL 244
M P++L+M+E+FGL LPRA HL A+PTYRL
Sbjct: 473 MLPSELDMSEEFGLALPRATHLHALPTYRL 502
Score = 38.5 bits (88), Expect(2) = e-101
Identities = 16/31 (51%), Positives = 23/31 (74%)
Frame = -1
Query: 1234 LQQGVSIAGAFNLADFFPYLRAFDLQGFGRR 1142
+Q+ + + GAFN+AD+ P+L A DLQG RR
Sbjct: 204 VQETLILVGAFNIADYVPFLGALDLQGLKRR 234
>ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinifera]
Length = 498
Score = 359 bits (921), Expect(2) = e-101
Identities = 174/266 (65%), Positives = 221/266 (83%)
Frame = -2
Query: 1041 RRMKAVNMVLDGFLEKIIDEHVQDAKELHGTHRDFIDVMLSLIESNNTWELHLGRDHIKA 862
RRMKA++ V D F EKIID+H+ + KE G +D IDVML + S E + R +IKA
Sbjct: 234 RRMKAISKVFDAFFEKIIDDHIHEPKE-EGQPKDLIDVMLGYMGSKEN-EFQIERSNIKA 291
Query: 861 IMVDMLAGTMDTSSTAIEWVIAELLKNPRAMKLVQEELQIVVGLDRIVEEADLIKLDYLK 682
+++DMLAG+MDTS+TAIEW +AELLKNPR MK VQEEL+ VVG++R VEE+DL L+YL
Sbjct: 292 LVLDMLAGSMDTSATAIEWALAELLKNPRIMKKVQEELEKVVGMERKVEESDLESLEYLD 351
Query: 681 MVIKESMRLHPVAPLLVPHESIEDITIKGYFIPKSSRVLINTWAIGRDPNVWSSNAEEFF 502
MV+KE++RLHPVAPLL+PHES+ED T+ G+ IP+ SRV++NT+AIGRDPNVW ++AE+F
Sbjct: 352 MVVKETLRLHPVAPLLIPHESLEDCTVNGFHIPQKSRVMVNTYAIGRDPNVW-TDAEKFL 410
Query: 501 PERFIGTNIDFQGRDFRFLPFGSGRRKCPGMQLGSTVVELVLAQLVHCFNLELPDGMSPN 322
PERFIG++ID +GRDF+ +PFGSGRR CPGMQLG TVV LVLAQLVHCF+ ELP+GM P+
Sbjct: 411 PERFIGSSIDLRGRDFQLIPFGSGRRGCPGMQLGLTVVRLVLAQLVHCFDWELPNGMMPS 470
Query: 321 DLEMTEKFGLTLPRANHLFAVPTYRL 244
+L+MTE+FGLT+PRA H+ AVPTYRL
Sbjct: 471 ELDMTEEFGLTVPRAKHILAVPTYRL 496
Score = 37.4 bits (85), Expect(2) = e-101
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = -1
Query: 1234 LQQGVSIAGAFNLADFFPYLRAFDLQGFGRR 1142
+Q+G+ +AGA N+ D+ P++ DLQG RR
Sbjct: 205 IQEGMQLAGAPNIGDYIPFVAPLDLQGLARR 235
>ref|XP_003634278.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 71D10-like [Vitis
vinifera]
Length = 506
Score = 355 bits (912), Expect(2) = e-100
Identities = 166/272 (61%), Positives = 217/272 (79%), Gaps = 6/272 (2%)
Frame = -2
Query: 1041 RRMKAVNMVLDGFLEKIIDEHVQDAKELHGTHRDFIDVMLSLIESNNTWELH------LG 880
RRMKA++ +D LEKIIDEH QDA E G H DF+DVMLSL+ N T H +
Sbjct: 233 RRMKAISGAVDHILEKIIDEHKQDASENQGNHNDFVDVMLSLM--NETKNFHQEPSYLIK 290
Query: 879 RDHIKAIMVDMLAGTMDTSSTAIEWVIAELLKNPRAMKLVQEELQIVVGLDRIVEEADLI 700
+++K I+ D++ G +DTS+T IEW+++EL ++PR M+ +QEEL+ V+G++R+VEE DL
Sbjct: 291 XENLKGIVWDIIVGAIDTSATTIEWLLSELFRHPRVMRQLQEELENVIGMERMVEEVDLA 350
Query: 699 KLDYLKMVIKESMRLHPVAPLLVPHESIEDITIKGYFIPKSSRVLINTWAIGRDPNVWSS 520
L YL MV+KE +RLHP PLL+PHESIEDIT+ GY+IPK S+++IN WAIGRDPN+WS+
Sbjct: 351 NLVYLYMVLKEGLRLHPAGPLLLPHESIEDITLNGYYIPKKSQIIINAWAIGRDPNIWSN 410
Query: 519 NAEEFFPERFIGTNIDFQGRDFRFLPFGSGRRKCPGMQLGSTVVELVLAQLVHCFNLELP 340
N E+FFPERFIG+NIDFQG+DF+F+PFGSGRRKCPGM LG + LVLAQLVHCF+ +LP
Sbjct: 411 NVEDFFPERFIGSNIDFQGKDFQFIPFGSGRRKCPGMHLGLINIRLVLAQLVHCFDWKLP 470
Query: 339 DGMSPNDLEMTEKFGLTLPRANHLFAVPTYRL 244
+GM P++L+M+E+FGL LPRA HL A+PTYRL
Sbjct: 471 NGMLPSELDMSEEFGLALPRATHLHALPTYRL 502
Score = 38.5 bits (88), Expect(2) = e-100
Identities = 16/31 (51%), Positives = 23/31 (74%)
Frame = -1
Query: 1234 LQQGVSIAGAFNLADFFPYLRAFDLQGFGRR 1142
+Q+ + + GAFN+AD+ P+L A DLQG RR
Sbjct: 204 VQETLILVGAFNIADYVPFLGALDLQGLKRR 234
>ref|XP_002511968.1| flavonoid 3-hydroxylase, putative [Ricinus communis]
gi|223549148|gb|EEF50637.1| flavonoid 3-hydroxylase,
putative [Ricinus communis]
Length = 501
Score = 340 bits (871), Expect(2) = 1e-95
Identities = 165/273 (60%), Positives = 212/273 (77%), Gaps = 5/273 (1%)
Frame = -2
Query: 1041 RRMKAVNMVLDGFLEKIIDEHVQDAKELHGTHRDFIDVMLSLIE----SNNTWELHLGRD 874
+R KA N D +EKIIDEH ++A + RDF+D +LS++ S++ E + R
Sbjct: 229 KRSKACNEAFDKIMEKIIDEHEKEAHWENKQQRDFVDALLSVVNQSMISHDGAESEIDRS 288
Query: 873 HIKAIMVDMLAGTMDTSSTAIEWVIAELLKNPRAMKLVQEELQIVVGLDRIVEEADLIKL 694
+IKAI++D++ +DTS+TAIEW +AEL+++P+AMK +Q+ELQ VVGLD++VEE DL KL
Sbjct: 289 NIKAILIDIIVAAVDTSATAIEWTLAELIRHPQAMKTLQDELQNVVGLDKMVEEKDLSKL 348
Query: 693 DYLKMVIKESMRLHPVAPLLVPHESIEDITIKGYFIPKSSRVLINTWAIGRDPNVWSSNA 514
YL MVIKES RLHPVAPLLVPHESI++ITI GY IPK SR+L+N WAIGRD NVWS N
Sbjct: 349 TYLDMVIKESSRLHPVAPLLVPHESIDEITIDGYHIPKRSRILVNIWAIGRDSNVWSDNV 408
Query: 513 EEFFPERFIGTNIDFQGRDFRFLPFGSGRRKCPGMQLGSTVVELVLAQLVHCFNLELPDG 334
+EF PERFIGTN+D G DFR +PFGSGRR CPG+ LG T V + +AQLVHCFN +LPDG
Sbjct: 409 DEFLPERFIGTNVDLHGHDFRLIPFGSGRRGCPGIHLGLTTVRMAIAQLVHCFNWKLPDG 468
Query: 333 -MSPNDLEMTEKFGLTLPRANHLFAVPTYRLRV 238
+SP++L+M+E+FGLT+ RA+HLF VP YRL V
Sbjct: 469 DVSPSELDMSEQFGLTVSRASHLFLVPEYRLPV 501
Score = 38.9 bits (89), Expect(2) = 1e-95
Identities = 16/33 (48%), Positives = 24/33 (72%)
Frame = -1
Query: 1240 SSLQQGVSIAGAFNLADFFPYLRAFDLQGFGRR 1142
S +++ + + GAFN+ DF P+L AFDLQG +R
Sbjct: 198 SVVEETLRLVGAFNIGDFVPFLGAFDLQGVKKR 230