BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00001992
         (3354 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267395.2| PREDICTED: ubiquitin-activating enzyme E1 1-...  1460   0.0  
emb|CBI18124.3| unnamed protein product [Vitis vinifera]             1458   0.0  
gb|AEK86562.1| ubiquitin activating enzyme E1 [Camellia sinensis]    1456   0.0  
emb|CAA71762.1| Ubiquitin activating enzyme E1 [Nicotiana tabacum]   1456   0.0  
ref|XP_002275617.2| PREDICTED: ubiquitin-activating enzyme E1 1 ...  1445   0.0  

>ref|XP_002267395.2| PREDICTED: ubiquitin-activating enzyme E1 1-like [Vitis vinifera]
          Length = 1111

 Score = 1460 bits (3779), Expect = 0.0
 Identities = 706/933 (75%), Positives = 788/933 (84%)
 Frame = +2

Query: 323  VSPLTGQLTPEQLSSFQAVVFTEISLKKAVEFDNYCHNHQPPISFXXXXXXXXXXXXXXX 502
            +S LT +LT EQLS FQAVVFT IS++KA+EFD+YCHNHQPPISF               
Sbjct: 190  ISTLTTELTKEQLSDFQAVVFTNISIEKAIEFDDYCHNHQPPISFIKSEVRGLFGSVFCD 249

Query: 503  XXXXXXXXXXXXXXXXXXXXXXXXXXXDVDGEEPHTGIVASIGNDNPALVSCVDDDRIEF 682
                                       DVDGE+PHTGI+ASI NDNPALV+CVDD+R+EF
Sbjct: 250  FGPEFTVF-------------------DVDGEDPHTGIIASISNDNPALVACVDDERLEF 290

Query: 683  QDGDLVVFSEVLGMTELNDGKPRKIKSTRPYSFILDEDTTNYGAYERGGIVTQVKQPKTL 862
            QDGDLVVFSEV GMTELNDGKPRK+K+ RPYSF LDEDTTNYGAYE+GGIVTQVKQPK L
Sbjct: 291  QDGDLVVFSEVQGMTELNDGKPRKVKNARPYSFSLDEDTTNYGAYEKGGIVTQVKQPKVL 350

Query: 863  HFKPLKEALGDPGDFLLSDFSKFDRPPLLHLAFQALDMFMSELGRFPVAGSEEDAQKLVS 1042
            +FKPLKEAL DPGDFL SDFSKFDR PLLHLAFQALD F+ ELGRFPVAGSEEDAQKL+S
Sbjct: 351  NFKPLKEALKDPGDFLQSDFSKFDRSPLLHLAFQALDKFIMELGRFPVAGSEEDAQKLIS 410

Query: 1043 FTININENSGDGRLEEIDRTILRHFAFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 1222
            F  NIN++S  G+LE+ID+ +L HF FGA+AVLNPMAAMFGG+VGQEVVKACSGKFHPLF
Sbjct: 411  FACNINDSSTVGKLEKIDQKLLHHFTFGARAVLNPMAAMFGGVVGQEVVKACSGKFHPLF 470

Query: 1223 QFFYFDSIESLPTKPLDPSDLRPLNSRYDAQISVFGSQFQKKLEQANIFIVGSGALGCEF 1402
            QFFYFDS+ESLPT+PLDPSDL+P+NSRYDAQISVFG++ QKKLE A +FIVGSGALGCEF
Sbjct: 471  QFFYFDSVESLPTEPLDPSDLKPINSRYDAQISVFGAKLQKKLEDAKVFIVGSGALGCEF 530

Query: 1403 LKNLALMGVCCSSKGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVXXXXXXXINPHI 1582
            LKN+ALMGVCC ++GKL ITDDDVIEKSNLSRQFLFRDWNIGQAKSTV       IN  +
Sbjct: 531  LKNVALMGVCCGNQGKLIITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAASINRRL 590

Query: 1583 HVEALQNRASSETENVFNDAFWENLDVVINALDNVNARIYMDQRCLYFQKPLLESGTLGA 1762
            H+EALQNRAS ETENVF+D FWENL VVINALDNVNAR+Y+DQRCLYFQKPLLESGTLGA
Sbjct: 591  HIEALQNRASPETENVFDDTFWENLSVVINALDNVNARLYIDQRCLYFQKPLLESGTLGA 650

Query: 1763 KCNTQMVIPHLTENYGASRDPPEKQAPMCTLHSFPHNIDHCLTWARSEFEGLFEKTPAEV 1942
            KCNTQMVIPHLTENYGASRDPPEKQAPMCT+HSFPHNIDHCLTWARSEFEGL EKTPAEV
Sbjct: 651  KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 710

Query: 1943 NAYLSNPIEYTSAMKKAGDAQARDNLEHVIDCLDRDRCETFQDCITWSRLKFEDHFSNRV 2122
            NA+L NPIEY SAMK AGDAQARDNLE VI+CLD++RCETFQDCITW+RLKFED+F+NRV
Sbjct: 711  NAFLVNPIEYASAMKNAGDAQARDNLERVIECLDKERCETFQDCITWARLKFEDYFANRV 770

Query: 2123 KQLTFTFPEDSITSTGAPFWSAPKRFPRPLQFSVDDPGHLNFIMAASILRAVTFGIPIPD 2302
            KQLTFTFPED+ TS GAPFWSAPKRFPRPLQFS+DDPG L+F+MAAS+LRA TFGIPIPD
Sbjct: 771  KQLTFTFPEDAATSNGAPFWSAPKRFPRPLQFSIDDPGQLHFVMAASVLRAETFGIPIPD 830

Query: 2303 WAKNPKKLSDAVNKVNIPDFQPKEGVRIDTDEKATNPTPASIDDVEVINDLTTRLVECRR 2482
            W K+P K +DAV+KV +PDF PK+ V+I TDEKAT+ + AS+DD  VIN+L  +L +C++
Sbjct: 831  WVKSPMKFADAVSKVIVPDFLPKKDVKIVTDEKATSLSTASVDDAAVINELIMKLEKCQK 890

Query: 2483 KLPPGFRMNPIQFEKDDDTNYHMDLIAGFANMRARNYSIPEVDKLKAKFXXXXXXXXXXX 2662
            KLPPGFRMNPIQFEKDDD+NYHMDLI+  ANMRARNYSIPEVDKLKAKF           
Sbjct: 891  KLPPGFRMNPIQFEKDDDSNYHMDLISALANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 950

Query: 2663 XXXXXXGLVCLELYKVLNGGHKVEDYRNTFANLALPLFSIAEPVPPKVIKHNELTWTVWD 2842
                  GLVCLELYKVL+GGHK+EDY+NTFANLALPLFS+AEPVPPKVIKH +++WTVWD
Sbjct: 951  STAMATGLVCLELYKVLHGGHKMEDYKNTFANLALPLFSMAEPVPPKVIKHQDMSWTVWD 1010

Query: 2843 RWILKGNPTLRELLQWLKDKGLNAYSISCGTCLLYNSMFPRHKERMDQKMVDLATEVAKV 3022
            RWIL  NPTLRELLQWL+DKGLNAYSIS G+CLLYNSMFPRHKERMD+K+VDLA E+ K 
Sbjct: 1011 RWILGDNPTLRELLQWLRDKGLNAYSISYGSCLLYNSMFPRHKERMDRKLVDLAKEIGKA 1070

Query: 3023 ELPPYRRHXXXXXXXXXXXXXXIDIPQVSIYFR 3121
            ELP YRRH              IDIPQ+SIYFR
Sbjct: 1071 ELPTYRRHFDVVVACEDDEDNDIDIPQISIYFR 1103



 Score =  148 bits (373), Expect = 1e-32
 Identities = 76/82 (92%), Positives = 79/82 (96%)
 Frame = +1

Query: 1   VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDEKTVELWDLSSNFIFSE 180
           VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDE +VELWDLSSNFIF+E
Sbjct: 107 VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDEGSVELWDLSSNFIFTE 166

Query: 181 KDVGKNRASASVQKLQELNNAV 246
            DVGKNRA ASVQKLQELNN+V
Sbjct: 167 DDVGKNRALASVQKLQELNNSV 188


>emb|CBI18124.3| unnamed protein product [Vitis vinifera]
          Length = 1066

 Score = 1458 bits (3774), Expect = 0.0
 Identities = 705/932 (75%), Positives = 787/932 (84%)
 Frame = +2

Query: 323  VSPLTGQLTPEQLSSFQAVVFTEISLKKAVEFDNYCHNHQPPISFXXXXXXXXXXXXXXX 502
            +S LT +LT EQLS FQAVVFT IS++KA+EFD+YCHNHQPPISF               
Sbjct: 105  ISTLTTELTKEQLSDFQAVVFTNISIEKAIEFDDYCHNHQPPISFIKSEVRGLFGSVFCD 164

Query: 503  XXXXXXXXXXXXXXXXXXXXXXXXXXXDVDGEEPHTGIVASIGNDNPALVSCVDDDRIEF 682
                                       DVDGE+PHTGI+ASI NDNPALV+CVDD+R+EF
Sbjct: 165  FGPEFTVF-------------------DVDGEDPHTGIIASISNDNPALVACVDDERLEF 205

Query: 683  QDGDLVVFSEVLGMTELNDGKPRKIKSTRPYSFILDEDTTNYGAYERGGIVTQVKQPKTL 862
            QDGDLVVFSEV GMTELNDGKPRK+K+ RPYSF LDEDTTNYGAYE+GGIVTQVKQPK L
Sbjct: 206  QDGDLVVFSEVQGMTELNDGKPRKVKNARPYSFSLDEDTTNYGAYEKGGIVTQVKQPKVL 265

Query: 863  HFKPLKEALGDPGDFLLSDFSKFDRPPLLHLAFQALDMFMSELGRFPVAGSEEDAQKLVS 1042
            +FKPLKEAL DPGDFL SDFSKFDR PLLHLAFQALD F+ ELGRFPVAGSEEDAQKL+S
Sbjct: 266  NFKPLKEALKDPGDFLQSDFSKFDRSPLLHLAFQALDKFIMELGRFPVAGSEEDAQKLIS 325

Query: 1043 FTININENSGDGRLEEIDRTILRHFAFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 1222
            F  NIN++S  G+LE+ID+ +L HF FGA+AVLNPMAAMFGG+VGQEVVKACSGKFHPLF
Sbjct: 326  FACNINDSSTVGKLEKIDQKLLHHFTFGARAVLNPMAAMFGGVVGQEVVKACSGKFHPLF 385

Query: 1223 QFFYFDSIESLPTKPLDPSDLRPLNSRYDAQISVFGSQFQKKLEQANIFIVGSGALGCEF 1402
            QFFYFDS+ESLPT+PLDPSDL+P+NSRYDAQISVFG++ QKKLE A +FIVGSGALGCEF
Sbjct: 386  QFFYFDSVESLPTEPLDPSDLKPINSRYDAQISVFGAKLQKKLEDAKVFIVGSGALGCEF 445

Query: 1403 LKNLALMGVCCSSKGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVXXXXXXXINPHI 1582
            LKN+ALMGVCC ++GKL ITDDDVIEKSNLSRQFLFRDWNIGQAKSTV       IN  +
Sbjct: 446  LKNVALMGVCCGNQGKLIITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAASINRRL 505

Query: 1583 HVEALQNRASSETENVFNDAFWENLDVVINALDNVNARIYMDQRCLYFQKPLLESGTLGA 1762
            H+EALQNRAS ETENVF+D FWENL VVINALDNVNAR+Y+DQRCLYFQKPLLESGTLGA
Sbjct: 506  HIEALQNRASPETENVFDDTFWENLSVVINALDNVNARLYIDQRCLYFQKPLLESGTLGA 565

Query: 1763 KCNTQMVIPHLTENYGASRDPPEKQAPMCTLHSFPHNIDHCLTWARSEFEGLFEKTPAEV 1942
            KCNTQMVIPHLTENYGASRDPPEKQAPMCT+HSFPHNIDHCLTWARSEFEGL EKTPAEV
Sbjct: 566  KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 625

Query: 1943 NAYLSNPIEYTSAMKKAGDAQARDNLEHVIDCLDRDRCETFQDCITWSRLKFEDHFSNRV 2122
            NA+L NPIEY SAMK AGDAQARDNLE VI+CLD++RCETFQDCITW+RLKFED+F+NRV
Sbjct: 626  NAFLVNPIEYASAMKNAGDAQARDNLERVIECLDKERCETFQDCITWARLKFEDYFANRV 685

Query: 2123 KQLTFTFPEDSITSTGAPFWSAPKRFPRPLQFSVDDPGHLNFIMAASILRAVTFGIPIPD 2302
            KQLTFTFPED+ TS GAPFWSAPKRFPRPLQFS+DDPG L+F+MAAS+LRA TFGIPIPD
Sbjct: 686  KQLTFTFPEDAATSNGAPFWSAPKRFPRPLQFSIDDPGQLHFVMAASVLRAETFGIPIPD 745

Query: 2303 WAKNPKKLSDAVNKVNIPDFQPKEGVRIDTDEKATNPTPASIDDVEVINDLTTRLVECRR 2482
            W K+P K +DAV+KV +PDF PK+ V+I TDEKAT+ + AS+DD  VIN+L  +L +C++
Sbjct: 746  WVKSPMKFADAVSKVIVPDFLPKKDVKIVTDEKATSLSTASVDDAAVINELIMKLEKCQK 805

Query: 2483 KLPPGFRMNPIQFEKDDDTNYHMDLIAGFANMRARNYSIPEVDKLKAKFXXXXXXXXXXX 2662
            KLPPGFRMNPIQFEKDDD+NYHMDLI+  ANMRARNYSIPEVDKLKAKF           
Sbjct: 806  KLPPGFRMNPIQFEKDDDSNYHMDLISALANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 865

Query: 2663 XXXXXXGLVCLELYKVLNGGHKVEDYRNTFANLALPLFSIAEPVPPKVIKHNELTWTVWD 2842
                  GLVCLELYKVL+GGHK+EDY+NTFANLALPLFS+AEPVPPKVIKH +++WTVWD
Sbjct: 866  STAMATGLVCLELYKVLHGGHKMEDYKNTFANLALPLFSMAEPVPPKVIKHQDMSWTVWD 925

Query: 2843 RWILKGNPTLRELLQWLKDKGLNAYSISCGTCLLYNSMFPRHKERMDQKMVDLATEVAKV 3022
            RWIL  NPTLRELLQWL+DKGLNAYSIS G+CLLYNSMFPRHKERMD+K+VDLA E+ K 
Sbjct: 926  RWILGDNPTLRELLQWLRDKGLNAYSISYGSCLLYNSMFPRHKERMDRKLVDLAKEIGKA 985

Query: 3023 ELPPYRRHXXXXXXXXXXXXXXIDIPQVSIYF 3118
            ELP YRRH              IDIPQ+SIYF
Sbjct: 986  ELPTYRRHFDVVVACEDDEDNDIDIPQISIYF 1017



 Score =  148 bits (373), Expect = 1e-32
 Identities = 76/82 (92%), Positives = 79/82 (96%)
 Frame = +1

Query: 1   VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDEKTVELWDLSSNFIFSE 180
           VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDE +VELWDLSSNFIF+E
Sbjct: 22  VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDEGSVELWDLSSNFIFTE 81

Query: 181 KDVGKNRASASVQKLQELNNAV 246
            DVGKNRA ASVQKLQELNN+V
Sbjct: 82  DDVGKNRALASVQKLQELNNSV 103


>gb|AEK86562.1| ubiquitin activating enzyme E1 [Camellia sinensis]
          Length = 1094

 Score = 1456 bits (3769), Expect = 0.0
 Identities = 704/933 (75%), Positives = 791/933 (84%)
 Frame = +2

Query: 323  VSPLTGQLTPEQLSSFQAVVFTEISLKKAVEFDNYCHNHQPPISFXXXXXXXXXXXXXXX 502
            +S LT +LT EQLS FQAVVFT+ISL+KA+EF++YCH+HQPPISF               
Sbjct: 181  ISTLTTELTKEQLSDFQAVVFTDISLEKAIEFNDYCHSHQPPISFIKTEVRGLFGSVFCD 240

Query: 503  XXXXXXXXXXXXXXXXXXXXXXXXXXXDVDGEEPHTGIVASIGNDNPALVSCVDDDRIEF 682
                                       DVDG +PHTGI+ASI NDNPA+V+CVDD+R+EF
Sbjct: 241  FGPEFTVF-------------------DVDGNDPHTGIIASISNDNPAIVACVDDERLEF 281

Query: 683  QDGDLVVFSEVLGMTELNDGKPRKIKSTRPYSFILDEDTTNYGAYERGGIVTQVKQPKTL 862
            +DGDLVVFSEV GM ELNDGKPRK+K+ RPYSF ++EDTTNY AYE+GGIVTQVKQPK L
Sbjct: 282  EDGDLVVFSEVHGMPELNDGKPRKVKNARPYSFTIEEDTTNYAAYEKGGIVTQVKQPKAL 341

Query: 863  HFKPLKEALGDPGDFLLSDFSKFDRPPLLHLAFQALDMFMSELGRFPVAGSEEDAQKLVS 1042
            +FKPL+EAL DPGDFLLSDFSKFDRPPLLHLAFQALDM++SELGRFP+AGSEEDAQKL+S
Sbjct: 342  NFKPLREALKDPGDFLLSDFSKFDRPPLLHLAFQALDMYISELGRFPIAGSEEDAQKLIS 401

Query: 1043 FTININENSGDGRLEEIDRTILRHFAFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 1222
               NIN +S  G+LEEID  +LR+F FGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF
Sbjct: 402  LATNINNSSASGKLEEIDPKLLRNFVFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 461

Query: 1223 QFFYFDSIESLPTKPLDPSDLRPLNSRYDAQISVFGSQFQKKLEQANIFIVGSGALGCEF 1402
            QFFYFDSIESLP +PLDPSDL+PLNSRYDAQISVFG++ QKKLE A +FIVGSGALGCEF
Sbjct: 462  QFFYFDSIESLPPEPLDPSDLKPLNSRYDAQISVFGAKLQKKLEDAKVFIVGSGALGCEF 521

Query: 1403 LKNLALMGVCCSSKGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVXXXXXXXINPHI 1582
            LKN+ALMGVCC ++GKLTITDDDVIEKSNL+RQFLFRDWNIGQAKSTV       INPH+
Sbjct: 522  LKNVALMGVCCGNQGKLTITDDDVIEKSNLTRQFLFRDWNIGQAKSTVAASAASLINPHL 581

Query: 1583 HVEALQNRASSETENVFNDAFWENLDVVINALDNVNARIYMDQRCLYFQKPLLESGTLGA 1762
            H++ALQNRAS ETENVF+D FWENL+VVINALDNV+AR+Y+DQRCLYFQKPLLESGTLGA
Sbjct: 582  HIDALQNRASPETENVFHDTFWENLNVVINALDNVSARLYIDQRCLYFQKPLLESGTLGA 641

Query: 1763 KCNTQMVIPHLTENYGASRDPPEKQAPMCTLHSFPHNIDHCLTWARSEFEGLFEKTPAEV 1942
            KCNTQMVIPHLTENYGASRDPPEKQAPMCT+HSFPHNIDHCLTWARSEFEGL EKTPAEV
Sbjct: 642  KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 701

Query: 1943 NAYLSNPIEYTSAMKKAGDAQARDNLEHVIDCLDRDRCETFQDCITWSRLKFEDHFSNRV 2122
            NAYL++P EYTSAMK AGDAQARDNLE VI+CLD+++CETFQDCITW+RLKFED+F+NRV
Sbjct: 702  NAYLTSPNEYTSAMKNAGDAQARDNLERVIECLDKEKCETFQDCITWARLKFEDYFANRV 761

Query: 2123 KQLTFTFPEDSITSTGAPFWSAPKRFPRPLQFSVDDPGHLNFIMAASILRAVTFGIPIPD 2302
            KQLTFTFPED++TS+G PFWSAPKRFPRPLQFSVDD  HL+F+ AASILRA TFGIPIPD
Sbjct: 762  KQLTFTFPEDAVTSSGTPFWSAPKRFPRPLQFSVDDTSHLHFVTAASILRAETFGIPIPD 821

Query: 2303 WAKNPKKLSDAVNKVNIPDFQPKEGVRIDTDEKATNPTPASIDDVEVINDLTTRLVECRR 2482
            W K+ KKL+DAVN+V +PDFQPK+ V+I TDEKAT+ + AS+DD  VIN+L  +L  C +
Sbjct: 822  WVKSSKKLADAVNRVIVPDFQPKKDVKIVTDEKATSLSTASMDDAVVINELVMKLEICHK 881

Query: 2483 KLPPGFRMNPIQFEKDDDTNYHMDLIAGFANMRARNYSIPEVDKLKAKFXXXXXXXXXXX 2662
            KL PGF+MNPIQFEKDDDTNYHMDLIAG ANMRARNYSIPEVDKLKAKF           
Sbjct: 882  KLMPGFKMNPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 941

Query: 2663 XXXXXXGLVCLELYKVLNGGHKVEDYRNTFANLALPLFSIAEPVPPKVIKHNELTWTVWD 2842
                  GLVCLELYK L+GGHK+EDYRNTFANLALPLFS+AEP+PPKVIKH +++WTVWD
Sbjct: 942  STAMATGLVCLELYKALDGGHKLEDYRNTFANLALPLFSMAEPLPPKVIKHQDMSWTVWD 1001

Query: 2843 RWILKGNPTLRELLQWLKDKGLNAYSISCGTCLLYNSMFPRHKERMDQKMVDLATEVAKV 3022
            RWI+  NPTLRELLQWLKDK LNAYSIS G+CLLYNSMFPRH+ERMD+KMVDLA EVAK 
Sbjct: 1002 RWIVGDNPTLRELLQWLKDKALNAYSISFGSCLLYNSMFPRHRERMDRKMVDLAREVAKA 1061

Query: 3023 ELPPYRRHXXXXXXXXXXXXXXIDIPQVSIYFR 3121
            ELPPYRRH              +DIPQVSIYFR
Sbjct: 1062 ELPPYRRHFDVVVACEDDEDNDVDIPQVSIYFR 1094



 Score =  144 bits (364), Expect = 1e-31
 Identities = 74/82 (90%), Positives = 77/82 (93%)
 Frame = +1

Query: 1   VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDEKTVELWDLSSNFIFSE 180
           VYGRETMRRLFASNVLISG+ GLGAEIAKNL+LAGVKSVTLHDE  VELWDLSSNFIFSE
Sbjct: 98  VYGRETMRRLFASNVLISGINGLGAEIAKNLVLAGVKSVTLHDEGIVELWDLSSNFIFSE 157

Query: 181 KDVGKNRASASVQKLQELNNAV 246
            DVGKNRA ASVQKLQELNN+V
Sbjct: 158 DDVGKNRALASVQKLQELNNSV 179


>emb|CAA71762.1| Ubiquitin activating enzyme E1 [Nicotiana tabacum]
          Length = 1080

 Score = 1456 bits (3768), Expect = 0.0
 Identities = 709/994 (71%), Positives = 808/994 (81%)
 Frame = +2

Query: 140  WNCGTCLAISFSQKKMLGKIVQVLLYRSCKN*TMR*XXXXXXXXXXXXXXXXXXXXXXXX 319
            WNCG  LAI F Q+KMLG+I  +LL R  +                              
Sbjct: 130  WNCGIYLAILFLQRKMLGRIGHLLLSRKLQE------------------------LNNAV 165

Query: 320  XVSPLTGQLTPEQLSSFQAVVFTEISLKKAVEFDNYCHNHQPPISFXXXXXXXXXXXXXX 499
             +S LT  LT EQLS+FQAVVFT+ISL+KAV+FD+YCH HQPPI+F              
Sbjct: 166  IISTLTDALTKEQLSNFQAVVFTDISLEKAVKFDDYCHQHQPPIAFIKAEVRGLFGSVFC 225

Query: 500  XXXXXXXXXXXXXXXXXXXXXXXXXXXXDVDGEEPHTGIVASIGNDNPALVSCVDDDRIE 679
                                        DVDGE+PHTGI+ASI NDNPALV C+DD+R+E
Sbjct: 226  DFGPEFTIA-------------------DVDGEDPHTGIIASISNDNPALVGCIDDERLE 266

Query: 680  FQDGDLVVFSEVLGMTELNDGKPRKIKSTRPYSFILDEDTTNYGAYERGGIVTQVKQPKT 859
            FQDGDLV+FSEV GMTELNDGKPRKIK+ RPYSF ++EDT+NY AYERGGIVTQVK+PK 
Sbjct: 267  FQDGDLVIFSEVRGMTELNDGKPRKIKNARPYSFTIEEDTSNYAAYERGGIVTQVKEPKV 326

Query: 860  LHFKPLKEALGDPGDFLLSDFSKFDRPPLLHLAFQALDMFMSELGRFPVAGSEEDAQKLV 1039
            L FKPL+EA+ DPGDFLLSDFSKFDRPP+LHLAFQALD F+SE GRFP+AGSEEDAQ+L+
Sbjct: 327  LKFKPLREAIKDPGDFLLSDFSKFDRPPILHLAFQALDRFVSESGRFPLAGSEEDAQRLI 386

Query: 1040 SFTININENSGDGRLEEIDRTILRHFAFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPL 1219
            SF  ++N +  DG+LEEID+ +LR+FAFGA+AVLNPMAAMFGGIVGQEVVKACSGKFHPL
Sbjct: 387  SFVTDLNNSLSDGKLEEIDQKLLRNFAFGARAVLNPMAAMFGGIVGQEVVKACSGKFHPL 446

Query: 1220 FQFFYFDSIESLPTKPLDPSDLRPLNSRYDAQISVFGSQFQKKLEQANIFIVGSGALGCE 1399
            +QFFYFDS+ESLPT PLDP+DL+PLNSRYDAQISVFG++ QKKLE+A  F+VGSGALGCE
Sbjct: 447  YQFFYFDSVESLPTAPLDPNDLKPLNSRYDAQISVFGNKLQKKLEEAKAFVVGSGALGCE 506

Query: 1400 FLKNLALMGVCCSSKGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVXXXXXXXINPH 1579
            FLKNLALMGVCC  KGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTV       INP 
Sbjct: 507  FLKNLALMGVCCGDKGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAAAAASLINPR 566

Query: 1580 IHVEALQNRASSETENVFNDAFWENLDVVINALDNVNARIYMDQRCLYFQKPLLESGTLG 1759
            IH+EALQNRAS ETE+VF+D FWENL VVINALDNVNAR+Y+DQRCLYFQKPLLESGTLG
Sbjct: 567  IHIEALQNRASPETESVFDDTFWENLSVVINALDNVNARLYIDQRCLYFQKPLLESGTLG 626

Query: 1760 AKCNTQMVIPHLTENYGASRDPPEKQAPMCTLHSFPHNIDHCLTWARSEFEGLFEKTPAE 1939
            AKCNTQMVIPHLTENYGASRDPPEKQAPMCT+HSFPHNIDHCLTWARSEFEGL EKTP E
Sbjct: 627  AKCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPTE 686

Query: 1940 VNAYLSNPIEYTSAMKKAGDAQARDNLEHVIDCLDRDRCETFQDCITWSRLKFEDHFSNR 2119
            VNAYL NP +Y SAM+KAGDAQARD L+ V++CLD++RC+TFQDCITW+RL+FED+F++R
Sbjct: 687  VNAYLINPSDYISAMQKAGDAQARDTLDRVLECLDKERCDTFQDCITWARLRFEDYFADR 746

Query: 2120 VKQLTFTFPEDSITSTGAPFWSAPKRFPRPLQFSVDDPGHLNFIMAASILRAVTFGIPIP 2299
            VKQLTFTFPE++ TS+GAPFWSAPKRFPRPLQFSVDD  HL F++AASILRA TFGI IP
Sbjct: 747  VKQLTFTFPEEATTSSGAPFWSAPKRFPRPLQFSVDDASHLQFLLAASILRAETFGILIP 806

Query: 2300 DWAKNPKKLSDAVNKVNIPDFQPKEGVRIDTDEKATNPTPASIDDVEVINDLTTRLVECR 2479
            DW K+P+KL++AV+KV +PDFQPK+ V+I TDEKAT+   +SIDD  VIN+L  +L  CR
Sbjct: 807  DWVKSPQKLAEAVDKVIVPDFQPKKDVKIVTDEKATSMAASSIDDAAVINELVMKLETCR 866

Query: 2480 RKLPPGFRMNPIQFEKDDDTNYHMDLIAGFANMRARNYSIPEVDKLKAKFXXXXXXXXXX 2659
            +KLP G++MNPIQFEKDDDTNYHMDLIAG ANMRARNYSIPEVDKLKAKF          
Sbjct: 867  QKLPSGYKMNPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIA 926

Query: 2660 XXXXXXXGLVCLELYKVLNGGHKVEDYRNTFANLALPLFSIAEPVPPKVIKHNELTWTVW 2839
                   GLVCLELYKVL+GGHKVEDYRNTFANLALPLFS+AEPVPPKV+KH ++ WTVW
Sbjct: 927  TSTAMATGLVCLELYKVLDGGHKVEDYRNTFANLALPLFSMAEPVPPKVVKHQDMNWTVW 986

Query: 2840 DRWILKGNPTLRELLQWLKDKGLNAYSISCGTCLLYNSMFPRHKERMDQKMVDLATEVAK 3019
            DRWILK NPTLRELLQWL++KGLNAYSIS G+CLLYNSMFP+HKERMD+K+VDLA EVAK
Sbjct: 987  DRWILKDNPTLRELLQWLQNKGLNAYSISYGSCLLYNSMFPKHKERMDRKLVDLAREVAK 1046

Query: 3020 VELPPYRRHXXXXXXXXXXXXXXIDIPQVSIYFR 3121
             +LPPYR+H              +DIPQ+SIYFR
Sbjct: 1047 ADLPPYRKHFDVVVACEDEEDNDVDIPQMSIYFR 1080



 Score = 94.7 bits (234), Expect = 1e-16
 Identities = 53/82 (64%), Positives = 59/82 (71%)
 Frame = +1

Query: 1   VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDEKTVELWDLSSNFIFSE 180
           VYGRETMRRLFASNVL+SG+QGLGAEIAKNLILAGVKSVTLHDE         +      
Sbjct: 84  VYGRETMRRLFASNVLVSGLQGLGAEIAKNLILAGVKSVTLHDEGMWNCGIYLAILFLQR 143

Query: 181 KDVGKNRASASVQKLQELNNAV 246
           K +G+       +KLQELNNAV
Sbjct: 144 KMLGRIGHLLLSRKLQELNNAV 165


>ref|XP_002275617.2| PREDICTED: ubiquitin-activating enzyme E1 1 [Vitis vinifera]
          Length = 1100

 Score = 1445 bits (3740), Expect = 0.0
 Identities = 706/932 (75%), Positives = 782/932 (83%)
 Frame = +2

Query: 323  VSPLTGQLTPEQLSSFQAVVFTEISLKKAVEFDNYCHNHQPPISFXXXXXXXXXXXXXXX 502
            +S LT +LT E LS FQAVVFT+I  +KA+EF++YCH+HQPPI+F               
Sbjct: 187  ISTLTTKLTKEDLSDFQAVVFTDIYFEKAIEFNDYCHSHQPPIAFIKAEVRGLFGSVFCD 246

Query: 503  XXXXXXXXXXXXXXXXXXXXXXXXXXXDVDGEEPHTGIVASIGNDNPALVSCVDDDRIEF 682
                                       DVDGEEPHTGI+ASI NDNPALVSCVDD+R+EF
Sbjct: 247  FGPEFTVF-------------------DVDGEEPHTGIIASISNDNPALVSCVDDERLEF 287

Query: 683  QDGDLVVFSEVLGMTELNDGKPRKIKSTRPYSFILDEDTTNYGAYERGGIVTQVKQPKTL 862
            QDGDLVVFSEV GMTELNDGKPRKIK+ RPYSF L+EDTTN+G YE+GGIVTQVKQPK L
Sbjct: 288  QDGDLVVFSEVHGMTELNDGKPRKIKNARPYSFTLEEDTTNFGTYEKGGIVTQVKQPKVL 347

Query: 863  HFKPLKEALGDPGDFLLSDFSKFDRPPLLHLAFQALDMFMSELGRFPVAGSEEDAQKLVS 1042
            +FKPL+EAL DPGDFLLSDFSKFDRPPLLHLAFQALD F+SELGRFPVAGSEEDAQKL+ 
Sbjct: 348  NFKPLREALSDPGDFLLSDFSKFDRPPLLHLAFQALDRFISELGRFPVAGSEEDAQKLIF 407

Query: 1043 FTININENSGDGRLEEIDRTILRHFAFGAKAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 1222
             + NINE  GDG+LE+I+  +LRHFAFGA+AVLNPMAAMFGGIVGQEVVKACSGKFHPLF
Sbjct: 408  ISSNINEGLGDGKLEDINPKLLRHFAFGARAVLNPMAAMFGGIVGQEVVKACSGKFHPLF 467

Query: 1223 QFFYFDSIESLPTKPLDPSDLRPLNSRYDAQISVFGSQFQKKLEQANIFIVGSGALGCEF 1402
            QFFYFDS+ESLPT+  D SD +PLNSRYDAQISVFGS+ QKKLE A +F+VGSGALGCEF
Sbjct: 468  QFFYFDSVESLPTEAPDSSDFKPLNSRYDAQISVFGSKLQKKLEDAVVFMVGSGALGCEF 527

Query: 1403 LKNLALMGVCCSSKGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVXXXXXXXINPHI 1582
            LKN+ALMGV C ++GKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTV       INP +
Sbjct: 528  LKNVALMGVSCGNQGKLTITDDDVIEKSNLSRQFLFRDWNIGQAKSTVAASAAQTINPCL 587

Query: 1583 HVEALQNRASSETENVFNDAFWENLDVVINALDNVNARIYMDQRCLYFQKPLLESGTLGA 1762
            H+EALQNR   ETENVFNDAFWENL VVINALDNVNAR+Y+DQRCLYFQKPLLESGTLGA
Sbjct: 588  HIEALQNRVGPETENVFNDAFWENLSVVINALDNVNARLYVDQRCLYFQKPLLESGTLGA 647

Query: 1763 KCNTQMVIPHLTENYGASRDPPEKQAPMCTLHSFPHNIDHCLTWARSEFEGLFEKTPAEV 1942
            KCNTQMVIPHLTENYGASRDPPEKQAPMCT+HSFPHNIDHCLTWARSEFEGL EKTPAEV
Sbjct: 648  KCNTQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPAEV 707

Query: 1943 NAYLSNPIEYTSAMKKAGDAQARDNLEHVIDCLDRDRCETFQDCITWSRLKFEDHFSNRV 2122
            NA+LSNP EY SAM+ AGDAQARDNLE V++CL+R+RCETFQDCITW+RL+FED+F NRV
Sbjct: 708  NAFLSNPTEYASAMRNAGDAQARDNLERVLECLERERCETFQDCITWARLRFEDYFVNRV 767

Query: 2123 KQLTFTFPEDSITSTGAPFWSAPKRFPRPLQFSVDDPGHLNFIMAASILRAVTFGIPIPD 2302
            KQL FTFPED+ TSTGAPFWSAPKRFP PLQFS  D GHL F+MAASILRA TFGIPIPD
Sbjct: 768  KQLIFTFPEDAATSTGAPFWSAPKRFPHPLQFSAADAGHLYFVMAASILRAETFGIPIPD 827

Query: 2303 WAKNPKKLSDAVNKVNIPDFQPKEGVRIDTDEKATNPTPASIDDVEVINDLTTRLVECRR 2482
            WAK+PKKL++AV+KV +P+FQPK  V+I TDEKAT+ + AS+DD  VIN+L  ++ +  +
Sbjct: 828  WAKHPKKLAEAVDKVIVPEFQPKTDVKIVTDEKATSLSTASVDDAAVINELLAKIEQSWK 887

Query: 2483 KLPPGFRMNPIQFEKDDDTNYHMDLIAGFANMRARNYSIPEVDKLKAKFXXXXXXXXXXX 2662
             LPPGFRMNPIQFEKDDDTNYHMDLIAG ANMRARNYSIPEVDKLKAKF           
Sbjct: 888  SLPPGFRMNPIQFEKDDDTNYHMDLIAGLANMRARNYSIPEVDKLKAKFIAGRIIPAIAT 947

Query: 2663 XXXXXXGLVCLELYKVLNGGHKVEDYRNTFANLALPLFSIAEPVPPKVIKHNELTWTVWD 2842
                  GLVCLELYKVL+GGHK+EDYRNTFANLALPLFS+AEPVPPKVIKH +++WTVWD
Sbjct: 948  STAMATGLVCLELYKVLDGGHKLEDYRNTFANLALPLFSMAEPVPPKVIKHRDMSWTVWD 1007

Query: 2843 RWILKGNPTLRELLQWLKDKGLNAYSISCGTCLLYNSMFPRHKERMDQKMVDLATEVAKV 3022
            RWILK NPTLRELLQWLKDKGLNAYSISCG+CLLYNSMFPRH+ERMD+K+VDLA EVAKV
Sbjct: 1008 RWILKDNPTLRELLQWLKDKGLNAYSISCGSCLLYNSMFPRHRERMDKKVVDLAREVAKV 1067

Query: 3023 ELPPYRRHXXXXXXXXXXXXXXIDIPQVSIYF 3118
            ELP YR H              IDIPQVSIYF
Sbjct: 1068 ELPAYRSHLDVVVACEDDEDNDIDIPQVSIYF 1099



 Score =  150 bits (378), Expect = 3e-33
 Identities = 76/82 (92%), Positives = 79/82 (96%)
 Frame = +1

Query: 1   VYGRETMRRLFASNVLISGMQGLGAEIAKNLILAGVKSVTLHDEKTVELWDLSSNFIFSE 180
           VYGRETMRRLFASNVL+SG+QGLGAEIAKNLILAGVKSVTLHDE TVELWD+SSNFIFSE
Sbjct: 104 VYGRETMRRLFASNVLVSGLQGLGAEIAKNLILAGVKSVTLHDEGTVELWDMSSNFIFSE 163

Query: 181 KDVGKNRASASVQKLQELNNAV 246
            DVGKNRA ASVQKLQELNNAV
Sbjct: 164 NDVGKNRALASVQKLQELNNAV 185