BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00001793
         (1724 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   791   0.0  
gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]   789   0.0  
ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demeth...   788   0.0  
gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]       787   0.0  
ref|XP_002274128.1| PREDICTED: sterol 14-demethylase [Vitis vini...   785   0.0  

>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
            gi|299735181|gb|ADJ37071.1| obtusifoliol
            14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  791 bits (2044), Expect = 0.0
 Identities = 390/487 (80%), Positives = 426/487 (87%)
 Frame = -1

Query: 1661 MDLKDNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVM 1482
            M+L DNK ++VG           L+S L++PRSKKRLPP+VKAWP++GGL+RF+KGPVVM
Sbjct: 1    MELGDNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVVKAWPIVGGLIRFLKGPVVM 60

Query: 1481 LREEYPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 1302
            LR+EYPKLGSVFTLN LNKNITFFIGPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVF
Sbjct: 61   LRQEYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVF 120

Query: 1301 DVDYTIRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYFSKWGDNGEVDLKYELEHLIILT 1122
            DVDYTIRQEQFRFFTEALRV KLKGYVDQMV EAE+YFSKWG++GEVDLKYELEHLIILT
Sbjct: 121  DVDYTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILT 180

Query: 1121 ASRCLLGREVRDKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAK 942
            ASRCLLG EVR+KLFDDVSALFHDLD+GMLPISVIFPYLPIPAHRRRD ARKKL+EIFA 
Sbjct: 181  ASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFAN 240

Query: 941  IISSRKETGKSENDMLQCFIESKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAY 762
            II+SRK TGK+ENDMLQCFI+SKYKDGRPTTE E+TGLLIAALFAGQHTSSITSTWTG+Y
Sbjct: 241  IINSRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSY 300

Query: 761  LLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHS 582
            LL N   +S V+DEQK LMKK+G+ VDHDILSEM+VLYR IKEA              HS
Sbjct: 301  LLTNDKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHS 360

Query: 581  DFSVTTREGKEYDIPKGHIVAASPAFANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSY 402
            +FSVTTREGKEYDI KGHIVA SPAFANRLPHIFK+PD YDP+RF  GREEDKAAGAFSY
Sbjct: 361  EFSVTTREGKEYDISKGHIVATSPAFANRLPHIFKNPDTYDPDRFGPGREEDKAAGAFSY 420

Query: 401  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYK 222
            ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFE ELISPFPEIDWNA          V+YK
Sbjct: 421  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYK 480

Query: 221  RRQLSLD 201
            RR+LS++
Sbjct: 481  RRKLSVE 487


>gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]
          Length = 487

 Score =  789 bits (2038), Expect = 0.0
 Identities = 389/487 (79%), Positives = 425/487 (87%)
 Frame = -1

Query: 1661 MDLKDNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVM 1482
            M+L DNK ++VG           L+S L++PRSKKRLPP++KA P++GGL+RF+KGP+VM
Sbjct: 1    MELGDNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVIKALPIVGGLIRFLKGPIVM 60

Query: 1481 LREEYPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 1302
            LR+EYPKLGSVFTLN LNKNITFFIGPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVF
Sbjct: 61   LRQEYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVF 120

Query: 1301 DVDYTIRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYFSKWGDNGEVDLKYELEHLIILT 1122
            DVDYTIRQEQFRFFTEALRV KLKGYVDQMV EAE+YFSKWG++GEVDLKYELEHLIILT
Sbjct: 121  DVDYTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILT 180

Query: 1121 ASRCLLGREVRDKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAK 942
            ASRCLLG EVR+KLFDDVSALFHDLD+GMLPISVIFPYLPIPAHRRRD ARKKL+EIFA 
Sbjct: 181  ASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFAN 240

Query: 941  IISSRKETGKSENDMLQCFIESKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAY 762
            II+SRK TGK+ENDMLQCFI+SKYKDGRPTTE E+TGLLIAALFAGQHTSSITSTWTG+Y
Sbjct: 241  IINSRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSY 300

Query: 761  LLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHS 582
            LL N   +S V+DEQK LMKK+G+ VDHDILSEMDVLYR IKEA              HS
Sbjct: 301  LLTNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHS 360

Query: 581  DFSVTTREGKEYDIPKGHIVAASPAFANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSY 402
            DFSVTTREGKEYDIPKGHIVA SPAFANRLPHIFK+P+ YDP+RF  GREEDKAAGAFSY
Sbjct: 361  DFSVTTREGKEYDIPKGHIVATSPAFANRLPHIFKNPESYDPDRFGPGREEDKAAGAFSY 420

Query: 401  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYK 222
            ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFE ELISPFPEIDWNA          V+YK
Sbjct: 421  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYK 480

Query: 221  RRQLSLD 201
            RR+LS +
Sbjct: 481  RRKLSAE 487


>ref|XP_002299356.1| cytochrome P450 obtusifoliol 14-alpha-demethylase [Populus
            trichocarpa] gi|222846614|gb|EEE84161.1| cytochrome P450
            obtusifoliol 14-alpha-demethylase [Populus trichocarpa]
          Length = 488

 Score =  788 bits (2034), Expect = 0.0
 Identities = 387/483 (80%), Positives = 426/483 (88%)
 Frame = -1

Query: 1649 DNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVMLREE 1470
            DNKF++VG           L+S L++PRS+KRLPP++K WP++GGL+RF+KGP+VMLREE
Sbjct: 6    DNKFLNVGLLIIATLLVAKLISALIMPRSQKRLPPVMKGWPLIGGLIRFLKGPIVMLREE 65

Query: 1469 YPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1290
            YPKLGSVFT+N +N+ ITF IGPEVSAHFFKASE DLSQQEVYQFNVPTFGPGVVFDV+Y
Sbjct: 66   YPKLGSVFTVNLVNRKITFLIGPEVSAHFFKASEVDLSQQEVYQFNVPTFGPGVVFDVEY 125

Query: 1289 TIRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYFSKWGDNGEVDLKYELEHLIILTASRC 1110
            +IRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYF KWGD+G VDLKYELEHLIILTASRC
Sbjct: 126  SIRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYFLKWGDSGVVDLKYELEHLIILTASRC 185

Query: 1109 LLGREVRDKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAKIISS 930
            LLGREVRDKLFDDV+ALFHDLD+GMLP+SV+FPYLPIPAHRRRD+ARKKL+EIFA II+S
Sbjct: 186  LLGREVRDKLFDDVAALFHDLDNGMLPVSVLFPYLPIPAHRRRDRARKKLAEIFANIINS 245

Query: 929  RKETGKSENDMLQCFIESKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLQN 750
            RK   KSENDMLQCFI+SKYKDGRPTTESE+TGLLIAALFAGQHTSSITSTWTGAYLL++
Sbjct: 246  RKLASKSENDMLQCFIDSKYKDGRPTTESEITGLLIAALFAGQHTSSITSTWTGAYLLRH 305

Query: 749  KNCLSEVLDEQKTLMKKYGDNVDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHSDFSV 570
               LS VL+EQK LMKK+G+ VDHDILSEMDVLYR IKEA              HSDFSV
Sbjct: 306  NEYLSAVLEEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSV 365

Query: 569  TTREGKEYDIPKGHIVAASPAFANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSYISFG 390
            TTR+GKEYDIPKGHIVA SPAFANRLPH+FKDPD YDP+RFA GREEDKAAGAFSYISFG
Sbjct: 366  TTRDGKEYDIPKGHIVATSPAFANRLPHVFKDPDSYDPDRFAYGREEDKAAGAFSYISFG 425

Query: 389  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKRRQL 210
            GGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNA          VRYKRR+L
Sbjct: 426  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAMVVGVKDKVMVRYKRREL 485

Query: 209  SLD 201
            S++
Sbjct: 486  SVN 488


>gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]
          Length = 487

 Score =  787 bits (2032), Expect = 0.0
 Identities = 388/487 (79%), Positives = 426/487 (87%)
 Frame = -1

Query: 1661 MDLKDNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVM 1482
            M+L DNKF++VG           L+S L++PRSKKRLPP++ +WP+LGGL+RF+KGPVVM
Sbjct: 1    MELSDNKFLNVGLLLVATLVVAKLMSALIMPRSKKRLPPVINSWPILGGLIRFLKGPVVM 60

Query: 1481 LREEYPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 1302
            LREEYPKLGSVFTLN LNKNITFFIGPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVF
Sbjct: 61   LREEYPKLGSVFTLNILNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVF 120

Query: 1301 DVDYTIRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYFSKWGDNGEVDLKYELEHLIILT 1122
            DVDYTIRQEQF FFTEALRV KLKGYVDQMV+EAE+YFSKWGD+GEVDLKYELEHLIILT
Sbjct: 121  DVDYTIRQEQFTFFTEALRVTKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILT 180

Query: 1121 ASRCLLGREVRDKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAK 942
            ASRCLLG+EV +KLF DVSALFHDLD+GMLPISVIFPYLPIPAHRRRD+ARKKL+EIFA 
Sbjct: 181  ASRCLLGQEVPNKLFADVSALFHDLDNGMLPISVIFPYLPIPAHRRRDKARKKLAEIFAN 240

Query: 941  IISSRKETGKSENDMLQCFIESKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAY 762
            II+SRK TGK+ENDMLQCFI+SKYKDGR TTE E+TGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241  IINSRKRTGKAENDMLQCFIDSKYKDGRATTEGEITGLLIAALFAGQHTSSITSTWTGAY 300

Query: 761  LLQNKNCLSEVLDEQKTLMKKYGDNVDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHS 582
            LL NK  +S V+DEQK LMKK+G+ VDHDILSEM+VLYR IKEA              HS
Sbjct: 301  LLCNKKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRHSHS 360

Query: 581  DFSVTTREGKEYDIPKGHIVAASPAFANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSY 402
            DF VTT+EGKEYDIPKGHIVA SPAFANRLPHI+K+PD YDP+RF+ GREEDKAAGAFSY
Sbjct: 361  DFCVTTKEGKEYDIPKGHIVATSPAFANRLPHIYKNPDTYDPDRFSPGREEDKAAGAFSY 420

Query: 401  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYK 222
            ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFE ELISPFPEIDWNA          V+YK
Sbjct: 421  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYK 480

Query: 221  RRQLSLD 201
            RR+LS +
Sbjct: 481  RRKLSTE 487


>ref|XP_002274128.1| PREDICTED: sterol 14-demethylase [Vitis vinifera]
          Length = 486

 Score =  785 bits (2028), Expect = 0.0
 Identities = 390/483 (80%), Positives = 423/483 (87%)
 Frame = -1

Query: 1649 DNKFMSVGXXXXXXXXXXXLVSTLLIPRSKKRLPPIVKAWPVLGGLVRFMKGPVVMLREE 1470
            DNKF +             L+S L+IPRSKKRLPP +KA+P++GGL+RF+KGPVVMLREE
Sbjct: 4    DNKFFNAAFLLVATLVVAKLISALIIPRSKKRLPPTIKAFPLIGGLIRFLKGPVVMLREE 63

Query: 1469 YPKLGSVFTLNFLNKNITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1290
            YPKLGSVFTL  LNKNI+FFIGP+VSAHFFKA ESDLSQQEVY+FNVP FGPGVVFDVDY
Sbjct: 64   YPKLGSVFTLKLLNKNISFFIGPDVSAHFFKAPESDLSQQEVYRFNVPIFGPGVVFDVDY 123

Query: 1289 TIRQEQFRFFTEALRVNKLKGYVDQMVVEAEDYFSKWGDNGEVDLKYELEHLIILTASRC 1110
            ++RQEQFRFFTEALRV KLKGYVDQMV+EAEDYFSKWGD GEVDLKYELEHLIILTASRC
Sbjct: 124  SVRQEQFRFFTEALRVTKLKGYVDQMVMEAEDYFSKWGDCGEVDLKYELEHLIILTASRC 183

Query: 1109 LLGREVRDKLFDDVSALFHDLDSGMLPISVIFPYLPIPAHRRRDQARKKLSEIFAKIISS 930
            LLG+E+R+KLF DVSALFHDLD+GMLPISVIFPYLPIPAHRRRDQARKKL+EIFA II+S
Sbjct: 184  LLGQEIRNKLFADVSALFHDLDNGMLPISVIFPYLPIPAHRRRDQARKKLAEIFANIIAS 243

Query: 929  RKETGKSENDMLQCFIESKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLQN 750
            RKETGKSENDMLQCFI SKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLL++
Sbjct: 244  RKETGKSENDMLQCFIASKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLRH 303

Query: 749  KNCLSEVLDEQKTLMKKYGDNVDHDILSEMDVLYRSIKEAXXXXXXXXXXXXXXHSDFSV 570
            K  LS V DEQ++LMKKYG  VDHDILSEMDVLYR IKEA              H+DFSV
Sbjct: 304  KEYLSAVQDEQRSLMKKYGSKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHTDFSV 363

Query: 569  TTREGKEYDIPKGHIVAASPAFANRLPHIFKDPDVYDPNRFAAGREEDKAAGAFSYISFG 390
            TTR+GKEYDIPKGHIVA SPAFANRLPHI+KDPD YDP+RFA GREEDKAAGAFSYISFG
Sbjct: 364  TTRDGKEYDIPKGHIVATSPAFANRLPHIYKDPDRYDPDRFAVGREEDKAAGAFSYISFG 423

Query: 389  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAXXXXXXXXXXVRYKRRQL 210
            GGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPE+DWNA          VRYKRR+L
Sbjct: 424  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEVDWNAMVVGVKGKVMVRYKRREL 483

Query: 209  SLD 201
             ++
Sbjct: 484  PVN 486