BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00000655
         (1344 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      691   0.0  
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   610   e-172
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       553   e-155
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   450   e-124
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   442   e-122

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  691 bits (1783), Expect = 0.0
 Identities = 340/358 (94%), Positives = 343/358 (95%)
 Frame = -3

Query: 1210 MAVEAKQPKKAAIXXXXXXXXXXXVPYDDIKHLIRSELARRLQWGYKPTYEEQTADIQNL 1031
            MAVEAKQ KKAAI           VPYDDIK LIR ELARRLQWGYKPTYEEQ A+IQNL
Sbjct: 1    MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNL 60

Query: 1030 THSLRQMKIATEVDTLDSQMYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 851
            THSLRQMKIATEV+TLDSQ+YEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL
Sbjct: 61   THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 120

Query: 850  YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 671
            YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN
Sbjct: 121  YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 180

Query: 670  VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 491
            VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA
Sbjct: 181  VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 240

Query: 490  YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR 311
            YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR
Sbjct: 241  YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR 300

Query: 310  LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 137
            LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK
Sbjct: 301  LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  610 bits (1573), Expect = e-172
 Identities = 299/361 (82%), Positives = 324/361 (89%), Gaps = 3/361 (0%)
 Frame = -3

Query: 1210 MAVEAKQ--PKKAAIXXXXXXXXXXXVPYDDIKHLIRSELARRLQWGYKPTYEEQTADIQ 1037
            MAVE KQ  PKKA I           VP D+IK LIR +L RRLQWG K TYEEQ A + 
Sbjct: 1    MAVEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLV 60

Query: 1036 NLTHSLRQMKIATEVDTLDSQMYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAML 857
            NLTHSLRQMKIATEV+TLD QMYE+PI+FLKIMNGSNLKGSCCYFK DSTTLDEAEIAML
Sbjct: 61   NLTHSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAML 120

Query: 856  DLYCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNL 677
            +LYCERAQI+DG SVLDLGCGQGALTL+VAQKYKN RVTAVTNSVSQKE+IEEESR+RNL
Sbjct: 121  ELYCERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNL 180

Query: 676  LNVEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKT 497
             NVEV LADITTH+M +TYDRILV+ELFEHMKNYELLLRKI EW++KDGLLF+EHICHKT
Sbjct: 181  SNVEVLLADITTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKT 240

Query: 496  FAYHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL 317
            FAYHYEP+D+DDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL
Sbjct: 241  FAYHYEPIDEDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL 300

Query: 316  KRLDANLDVIKPMFETLMGN-EEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 140
            KRLDAN+++IKPMF T+ G   +EA+KLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK
Sbjct: 301  KRLDANVELIKPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 360

Query: 139  K 137
            K
Sbjct: 361  K 361


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  553 bits (1424), Expect = e-155
 Identities = 260/333 (78%), Positives = 295/333 (88%)
 Frame = -3

Query: 1135 PYDDIKHLIRSELARRLQWGYKPTYEEQTADIQNLTHSLRQMKIATEVDTLDSQMYEIPI 956
            P D++K L+R +LARRLQWGYK TYEEQ A + +LTHSLR M IA EVDTLDS MYE+P+
Sbjct: 19   PNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVDTLDSNMYEVPL 78

Query: 955  EFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTL 776
            +FLKIMNGS LK S CYFK+DSTTLDEAEIAM+DL+CERAQI+DG SVLDLGCG G+LT 
Sbjct: 79   DFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVLDLGCGHGSLTF 138

Query: 775  HVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIEL 596
            +VAQKYK   VTAVTNSVSQKEYIEEE+RRRNL NVEV LADI THEMA+TYDRILV+ L
Sbjct: 139  YVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMADTYDRILVVGL 198

Query: 595  FEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIP 416
            FEHMKNY LLL+KISEW++KDGLLF++H+CHKTFA+H EP+D+DDW +EY FPAGTMIIP
Sbjct: 199  FEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSEYAFPAGTMIIP 258

Query: 415  SASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKL 236
            SASFFLYFQDD SVVNHWTLSGKHFSRT EEWLKRLDAN+ VI PMF T+ G+EEEAVKL
Sbjct: 259  SASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFATITGSEEEAVKL 318

Query: 235  INYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 137
            +NYWRG C  G+E++GYNNGEEWM SHVLFKKK
Sbjct: 319  LNYWRGLCFFGVELYGYNNGEEWMESHVLFKKK 351


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  450 bits (1158), Expect = e-124
 Identities = 207/333 (62%), Positives = 265/333 (79%)
 Frame = -3

Query: 1135 PYDDIKHLIRSELARRLQWGYKPTYEEQTADIQNLTHSLRQMKIATEVDTLDSQMYEIPI 956
            P  +I+ LIR EL +RLQWGYK T+EEQ + + +L HSL+ MK+ATE++ LD ++YE P+
Sbjct: 19   PDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENLDLKLYEAPM 78

Query: 955  EFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTL 776
            EFLKI +GSN+K S  Y+ ++STTLDEAEIAMLDLY ERAQI+DGQSVLDLGCG GA+ L
Sbjct: 79   EFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDLGCGLGAVAL 138

Query: 775  HVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIEL 596
              A K+K C+ T VT+SV QK+YIE + +   L NV+V LADITT+E  E +DRI  +EL
Sbjct: 139  FGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETEERFDRIFAVEL 198

Query: 595  FEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIP 416
             EHMKNY+LLL+KISEW+  DGLLF+EH+CHKT AYHYEP+D +DW+T Y+FPAGT+ + 
Sbjct: 199  IEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYIFPAGTLTLS 258

Query: 415  SASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKL 236
            SAS  LYFQDDVSVVN WTLSGKH+SR++EEWLK +D N+   K +  ++   E+EA+KL
Sbjct: 259  SASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRSITKTEKEAIKL 318

Query: 235  INYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 137
            +N+WR FC+ G E+FGY NGEEWM +H+LFKKK
Sbjct: 319  LNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 351


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  442 bits (1138), Expect = e-122
 Identities = 204/356 (57%), Positives = 274/356 (76%)
 Frame = -3

Query: 1204 VEAKQPKKAAIXXXXXXXXXXXVPYDDIKHLIRSELARRLQWGYKPTYEEQTADIQNLTH 1025
            +E KQ KK A+           V  ++I+ +++ ++ +RL+WGYKPT+E+Q A +     
Sbjct: 1    METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60

Query: 1024 SLRQMKIATEVDTLDSQMYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYC 845
            SL+ M++A EVDTLD+++YEIP+ FL IM G  LK S  YFK++STTLDE+E+ M+DLYC
Sbjct: 61   SLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYC 120

Query: 844  ERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVE 665
            ERAQI+DGQS+LDLGCG G+LTLHVAQKY+ C+VT +TNSVSQKE+I ++ ++ +L NVE
Sbjct: 121  ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVE 180

Query: 664  VKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYH 485
            + L D+T  E   TYDRI  + L EHMKNYEL L+K+S WI++ GLLF+EH CHK FAY 
Sbjct: 181  IILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQ 240

Query: 484  YEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLD 305
            YEPLD+DDW+TEY+FP+GT+++ S+S  LYFQ+DVSVVNHWTLSGKH S   ++WLKRLD
Sbjct: 241  YEPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLD 300

Query: 304  ANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 137
             N+D +K +FE+  G++E+A+K I YWR FC++  +M+  NNGEEWM S VLFKKK
Sbjct: 301  DNIDEVKEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356