BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00000613
         (1417 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...   576   e-162
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        292   1e-76
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   267   6e-69
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   265   2e-68
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   264   4e-68

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT; AltName:
            Full=Tetrahydrocolumbamine 2-O-methyltransferase
            gi|24210466|dbj|BAC22084.1| columbamine
            O-methyltransferase [Coptis japonica]
          Length = 351

 Score =  576 bits (1485), Expect = e-162
 Identities = 287/353 (81%), Positives = 317/353 (89%), Gaps = 1/353 (0%)
 Frame = +2

Query: 125  MDASKQFQNE-EVNAQVQVWKHMYGFAETIMLRSTVNLGISDIIHNQGPVTLSQLATHLP 301
            MD    FQN+ E+ AQ QVWKHM+GFAETIMLRSTV+LGI DIIHN GPVTLSQL THLP
Sbjct: 1    MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 60

Query: 302  LNSISIDRLHHFMRYLVHMKLFSTSTDDDITKEIKYELTPASKLLVKGHKRSLAPYVLLQ 481
            L S SIDR HHFMRYLVHM+LF+ STD  ITKE KYELTPASKLLV GH++SLAPYV+LQ
Sbjct: 61   LKSTSIDRFHHFMRYLVHMQLFTISTDQ-ITKEDKYELTPASKLLVHGHQKSLAPYVMLQ 119

Query: 482  THPEEYSIWGHVIDMLDGKKPYWESTYGISVYEKSEGDPEINEILNDAMTSHGEFMVPAL 661
            THPEE+S+W HVI++LDGKKPYWES    S+YEK+EGDPEINEILNDAMTSH  FM+PAL
Sbjct: 120  THPEEFSVWSHVINVLDGKKPYWESN-DTSMYEKTEGDPEINEILNDAMTSHSTFMLPAL 178

Query: 662  VSGMMKENVLEGIGSIVDVGGNSGVVAKAIAEAFPHVKCSVMDLNHVIESVIKDPMLDFV 841
            VSG+MKENVL+G+ SIVDVGGNSGVVAK I +AFPHVKCSVMDLNHVIE VIK+P LD+V
Sbjct: 179  VSGLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYV 238

Query: 842  AGDMFTSVPNADAVLLKSTLHNYEDDDCVKILSKAKEAMPSTGGKVILVEIVVDIENLPQ 1021
            AGDMFTS+PNADA+LLKSTLHNYEDDDC+KIL+ AKEA+PSTGGKVILVEIVVD ENLP 
Sbjct: 239  AGDMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPL 298

Query: 1022 FTSARLSMEVEMMLMGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAYS 1180
            FTSARLSM ++MMLM GKERTKKEWEDLLRKA+FT HQVIPIMAIESIIVAYS
Sbjct: 299  FTSARLSMGMDMMLMSGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAYS 351


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  292 bits (748), Expect = 1e-76
 Identities = 143/339 (42%), Positives = 227/339 (66%), Gaps = 2/339 (0%)
 Frame = +2

Query: 167  QVQVWKHMYGFAETIMLRSTVNLGISDIIHNQG-PVTLSQLATHLPLNSISIDRLHHFMR 343
            Q ++WKH+YGFAE+++L+S V L I+D IHN G P+TLS+LA+ LP+  + +DRL+  MR
Sbjct: 14   QAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMR 73

Query: 344  YLVHMKLFSTSTDDDITKEIKYELTPASKLLVKGHKRSLAPYVLLQTHPEEYSIWGHVID 523
            YLVHMKLF    D+D  + IKY L P +K L+KG ++S+   +LL    +  S W H+ +
Sbjct: 74   YLVHMKLFKKQEDED-EESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHHLKE 132

Query: 524  MLDGKKPYWESTYGISVYEKSEGDPEINEILNDAMTSHGEFMVPALVSGMMKENVLEGIG 703
             L      ++   G S+++    +P+ N++ N+ M      +  ALV+     N+ +GI 
Sbjct: 133  GLVNNSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNEC--GNIFQGIQ 190

Query: 704  SIVDVGGNSGVVAKAIAEAFPHVKCSVMDLNHVIESVIKDPMLDFVAGDMFTSVPNADAV 883
            ++VDVGG +G   KA+++AFPH+KCS+ DL HVI      P +  + G+MF S+P+ADA+
Sbjct: 191  TLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPSADAI 250

Query: 884  LLKSTLHNYEDDDCVKILSKAKEAMPSTGGKVILVEIVVDIENLPQFTSARLSMEVEMML 1063
            L+K  LH++ DD+C++IL K KEA+P  GGKVI+V+IV+D+E+   ++  RL+++++MML
Sbjct: 251  LMKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDLDMML 310

Query: 1064 -MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 1177
               GKERT++EW+ L+  A F  +++  I A++S+I A+
Sbjct: 311  NTEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAF 349


>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  267 bits (682), Expect = 6e-69
 Identities = 133/343 (38%), Positives = 219/343 (63%), Gaps = 2/343 (0%)
 Frame = +2

Query: 155  EVNAQVQVWKHMYGFAETIMLRSTVNLGISDIIHNQG-PVTLSQLATHLPLNSISIDRLH 331
            ++ AQ  VWK +YGFA++++LR  V LGI DI+ N   P+ L+ LA+ LP+++++ D L+
Sbjct: 11   DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLPVSNVNCDNLY 70

Query: 332  HFMRYLVHMKLFSTSTDDDITKEIKYELTPASKLLVKGHKRSLAPYVLLQTHPEEYSIWG 511
              +RYLV M++      DD  K  KY L P + LL +  KRS+ P +L  T  +  + W 
Sbjct: 71   RILRYLVKMEILRVEKSDDGEK--KYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 128

Query: 512  HVIDMLDGKKPYWESTYGISVYEKSEGDPEINEILNDAMTSHGEFMVPALVSGMMKENVL 691
             + D L      +E   G++++E  EG P+ +++ N+ M      +  +L+SG    ++ 
Sbjct: 129  SMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISG--SRDMF 186

Query: 692  EGIGSIVDVGGNSGVVAKAIAEAFPHVKCSVMDLNHVIESVIKDPMLDFVAGDMFTSVPN 871
            +GI S+VDVGG +G   KAI++AFPH+KC++ DL HVI +    P ++ + GDMF SVP+
Sbjct: 187  QGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPS 246

Query: 872  ADAVLLKSTLHNYEDDDCVKILSKAKEAMPSTGGKVILVEIVVDIENLPQFTSARLSMEV 1051
            A A+LLK  LH++ D+D +KIL + + A+P  GGKVI+V++ +D E+  + +S RL +++
Sbjct: 247  AQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDI 306

Query: 1052 EMML-MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 1177
            +M++  GGKERTK+ WE +++ A F+  ++  I AI+S+I  +
Sbjct: 307  DMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVF 349


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
            4'-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:3'-hydroxy-N-
            methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
            gi|9711916|dbj|BAB08005.1|
            S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
            4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  265 bits (677), Expect = 2e-68
 Identities = 133/343 (38%), Positives = 218/343 (63%), Gaps = 2/343 (0%)
 Frame = +2

Query: 155  EVNAQVQVWKHMYGFAETIMLRSTVNLGISDIIHNQG-PVTLSQLATHLPLNSISIDRLH 331
            ++ AQ  VWK +YGFA++++LR  V LGI DII N   P+ L+ LA+ LP++ ++ D L+
Sbjct: 11   DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLY 70

Query: 332  HFMRYLVHMKLFSTSTDDDITKEIKYELTPASKLLVKGHKRSLAPYVLLQTHPEEYSIWG 511
              +RYLV M++      DD  K  KY L P + LL +  KRS+ P +L  T  +  + W 
Sbjct: 71   RILRYLVKMEILRVEKSDDGQK--KYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 128

Query: 512  HVIDMLDGKKPYWESTYGISVYEKSEGDPEINEILNDAMTSHGEFMVPALVSGMMKENVL 691
             + D L      +E   G++++E  EG P+ +++ N+ M      +  +L+SG    ++ 
Sbjct: 129  SMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISG--SRDMF 186

Query: 692  EGIGSIVDVGGNSGVVAKAIAEAFPHVKCSVMDLNHVIESVIKDPMLDFVAGDMFTSVPN 871
            +GI S+VDVGG +G   KAI++AFPH+KC++ DL HVI +    P ++ + GDMF SVP+
Sbjct: 187  QGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPS 246

Query: 872  ADAVLLKSTLHNYEDDDCVKILSKAKEAMPSTGGKVILVEIVVDIENLPQFTSARLSMEV 1051
            A A++LK  LH++ D+D +KIL + + A+P  GGKVI+V++ +D E+  + +S RL +++
Sbjct: 247  AQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDI 306

Query: 1052 EMML-MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 1177
            +M++  GGKERTK+ WE +++ A F+  ++  I AI+S+I  +
Sbjct: 307  DMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVF 349


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  264 bits (675), Expect = 4e-68
 Identities = 135/349 (38%), Positives = 225/349 (64%), Gaps = 3/349 (0%)
 Frame = +2

Query: 140  QFQNEEVNAQVQVWKHMYGFAETIMLRSTVNLGISDIIHNQGP-VTLSQLATHLPLNSIS 316
            + + + +++Q ++W  +YGFAE+++L+  V L +++IIHN G  +TLS+L++ LP   ++
Sbjct: 2    EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVN 61

Query: 317  IDRLHHFMRYLVHMKLFSTSTDDDITKEIKYELTPASKLLVKGHKRSLAPYVLLQTHPEE 496
             D L+  MRYLVHMKLF+ ++ D    E++Y L P +K LVKG  + +   +L  T  + 
Sbjct: 62   EDALYRVMRYLVHMKLFTKASIDG---ELRYGLAPPAKYLVKGWDKCMVGSILAITDKDF 118

Query: 497  YSIWGHVIDMLDGKK-PYWESTYGISVYEKSEGDPEINEILNDAMTSHGEFMVPALVSGM 673
             + W ++ D L G+    +E   G +++      PE N++ N+AM +    ++ ALV   
Sbjct: 119  MAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKEC 178

Query: 674  MKENVLEGIGSIVDVGGNSGVVAKAIAEAFPHVKCSVMDLNHVIESVIKDPMLDFVAGDM 853
               N+  GI ++VDVGG +G   + IA AFPH+KC+V DL HVI        +  VAGDM
Sbjct: 179  --GNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDM 236

Query: 854  FTSVPNADAVLLKSTLHNYEDDDCVKILSKAKEAMPSTGGKVILVEIVVDIENLPQFTSA 1033
            F  +P ADA+++K  LH+++D +C++IL + KEA+P  GGKVI+V+IV+++++   +T  
Sbjct: 237  FKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKM 296

Query: 1034 RLSMEVEMML-MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 1177
            RL+++++MML  GGKERT++EW+ L+  A +  H++  I A++S+I AY
Sbjct: 297  RLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345