BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00000586
         (993 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|A2A1A0.1|NCS1_COPJA RecName: Full=S-norcoclaurine synthase 1;...   343   3e-92
ref|XP_002527641.1| Flavonol synthase/flavanone 3-hydroxylase, p...   301   1e-79
ref|XP_002282515.2| PREDICTED: S-norcoclaurine synthase 1-like i...   282   1e-73
emb|CAN69330.1| hypothetical protein VITISV_016336 [Vitis vinifera]   281   2e-73
ref|XP_002279355.1| PREDICTED: S-norcoclaurine synthase 1 [Vitis...   279   8e-73

>sp|A2A1A0.1|NCS1_COPJA RecName: Full=S-norcoclaurine synthase 1; Short=CjNCS1
           gi|123720767|dbj|BAF45337.1| norcoclaurine synthase
           [Coptis japonica var. dissecta]
           gi|301072256|gb|ADK56103.1| 2OG/Fe(II)-dependent
           dioxygenase-like protein [synthetic construct]
          Length = 352

 Score =  343 bits (881), Expect = 3e-92
 Identities = 171/267 (64%), Positives = 204/267 (76%)
 Frame = +2

Query: 191 MTKNLISLGGSLPVDNVQALAGTELKDVPDRYVRSELEYDNDVVSTDNDVEIPVIDLSRL 370
           M+KNL  +GGSLPV+NVQ LAG ELK++P+RYVR ELE+D DVV  DN +EIPVIDLSRL
Sbjct: 1   MSKNLTGVGGSLPVENVQVLAGKELKNLPNRYVRPELEHD-DVVPIDNSLEIPVIDLSRL 59

Query: 371 LNQKSASDELAKFHSACLDWGFFQLINHGVSEEVIEKMKVDMEDFFRLPLEEKKVYGQLP 550
           L+Q+ A DELAKFHSACLDWGFFQLINHGV EEVIEKMKVD EDFFRLP +EK  Y QLP
Sbjct: 60  LDQQYACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLP 119

Query: 551 DSTEGYGQSFVKSDEQKLEWADMHLLITNPVKERNMRFWPNNPTSFRESVNKFSKDVQEV 730
           +  EGYGQ+FV S+EQKL+WADMH LIT PV+ERNMRFWP +PTSFRE++ K+S ++Q+V
Sbjct: 120 NGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKV 179

Query: 731 AMCLIGLMARNLGLESQVLTKPFENCIQAVRMNYYPPCRYSDKVLGLTPHSDASSXXXXX 910
           AMCL G+MA+NLGLES++LTKP             P      + LGL+PHSDA+      
Sbjct: 180 AMCLTGMMAKNLGLESEILTKPLRTVFNR-EDELLPSMSSCGEGLGLSPHSDATGLTLLI 238

Query: 911 XXXXXXXXXIKKNGKWFPVQPILGAFV 991
                    IKK+ KW P++PILGAFV
Sbjct: 239 QVNEVNGLHIKKDEKWVPIKPILGAFV 265


>ref|XP_002527641.1| Flavonol synthase/flavanone 3-hydroxylase, putative [Ricinus
           communis] gi|223532946|gb|EEF34712.1| Flavonol
           synthase/flavanone 3-hydroxylase, putative [Ricinus
           communis]
          Length = 359

 Score =  301 bits (772), Expect = 1e-79
 Identities = 142/262 (54%), Positives = 192/262 (73%), Gaps = 1/262 (0%)
 Frame = +2

Query: 209 SLGGSLPVDNVQALAGTELKDVPDRYVRSELEYDNDVVSTDNDVEIPVIDLSRLL-NQKS 385
           + GGSLPV+NVQAL+   LK+VP RY+R ELE+D    S D  ++IPVID+S+L  +Q S
Sbjct: 9   NFGGSLPVENVQALSSKNLKEVPIRYIRPELEFDE--FSMDESLQIPVIDMSKLKEDQSS 66

Query: 386 ASDELAKFHSACLDWGFFQLINHGVSEEVIEKMKVDMEDFFRLPLEEKKVYGQLPDSTEG 565
            +DELA+ H AC +WGFFQLINHGVSEEV+E MK+D+++FF+LP EEK  + QLP++ EG
Sbjct: 67  HNDELAQLHIACRNWGFFQLINHGVSEEVMENMKMDIQEFFKLPFEEKMAFAQLPNNIEG 126

Query: 566 YGQSFVKSDEQKLEWADMHLLITNPVKERNMRFWPNNPTSFRESVNKFSKDVQEVAMCLI 745
           YGQ+FV SDEQKL+W DM  L+  P   R MRFWP NPTSF E+ +K+S ++Q +A+C++
Sbjct: 127 YGQAFVVSDEQKLDWGDMLFLLPLPASSRKMRFWPTNPTSFGETFDKYSSELQRIAVCIL 186

Query: 746 GLMARNLGLESQVLTKPFENCIQAVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXX 925
            LMARNLG++ + +   FE+ +Q +RMNYYPPC  ++KV+GLT HSDA+           
Sbjct: 187 RLMARNLGIDPEDVATMFEDGVQGIRMNYYPPCIQANKVIGLTTHSDATGLTLLTQVNEV 246

Query: 926 XXXXIKKNGKWFPVQPILGAFV 991
               IKK+G+W P+ PI GAF+
Sbjct: 247 QGLQIKKDGRWVPITPIPGAFI 268


>ref|XP_002282515.2| PREDICTED: S-norcoclaurine synthase 1-like isoform 2 [Vitis
           vinifera]
          Length = 373

 Score =  282 bits (721), Expect = 1e-73
 Identities = 139/269 (51%), Positives = 185/269 (68%)
 Frame = +2

Query: 185 ERMTKNLISLGGSLPVDNVQALAGTELKDVPDRYVRSELEYDNDVVSTDNDVEIPVIDLS 364
           E  TK    + GSLPV NVQALA +   DVP RY+R EL   ++ V  D  ++IP +D+ 
Sbjct: 21  ESGTKRRQKMVGSLPVANVQALASSYSGDVPLRYLRPELL--SEEVLVDESLQIPTVDMR 78

Query: 365 RLLNQKSASDELAKFHSACLDWGFFQLINHGVSEEVIEKMKVDMEDFFRLPLEEKKVYGQ 544
           +LL      DE++K H AC +WGFFQLINHG +EEVIEKMK D+++FF+LPL+EK  Y +
Sbjct: 79  KLLVD---DDEMSKLHLACKEWGFFQLINHGAAEEVIEKMKADVQEFFKLPLKEKNAYAK 135

Query: 545 LPDSTEGYGQSFVKSDEQKLEWADMHLLITNPVKERNMRFWPNNPTSFRESVNKFSKDVQ 724
           LP+  EGYGQ+FV S++QKL+WADMH L + P  ERNMRFWP  PTSFR ++ K+S ++ 
Sbjct: 136 LPNGVEGYGQNFVVSEDQKLDWADMHFLQSLPASERNMRFWPEEPTSFRGTLEKYSLELV 195

Query: 725 EVAMCLIGLMARNLGLESQVLTKPFENCIQAVRMNYYPPCRYSDKVLGLTPHSDASSXXX 904
           +V+ CL+ LMA+NL +  + LT  F+   QAVRMNYYPPC ++ KV+GLTPHSD      
Sbjct: 196 KVSNCLLKLMAKNLLINPEQLTNMFDVGRQAVRMNYYPPCVHASKVIGLTPHSDFGGLTL 255

Query: 905 XXXXXXXXXXXIKKNGKWFPVQPILGAFV 991
                      IK+NGKW P++P+ GAF+
Sbjct: 256 LVQVNEVQGLQIKRNGKWIPIRPVPGAFI 284


>emb|CAN69330.1| hypothetical protein VITISV_016336 [Vitis vinifera]
          Length = 344

 Score =  281 bits (719), Expect = 2e-73
 Identities = 138/258 (53%), Positives = 181/258 (70%)
 Frame = +2

Query: 218 GSLPVDNVQALAGTELKDVPDRYVRSELEYDNDVVSTDNDVEIPVIDLSRLLNQKSASDE 397
           GS PV NVQALA +   DVP RY+R EL   ++ V  D  ++IP ID+ +LL      DE
Sbjct: 3   GSXPVANVQALASSYSGDVPLRYLRPELL--SEEVLVDESLQIPTIDMRKLLVD---DDE 57

Query: 398 LAKFHSACLDWGFFQLINHGVSEEVIEKMKVDMEDFFRLPLEEKKVYGQLPDSTEGYGQS 577
           ++K H AC +WGFFQLINHG +EEVIEKMK D+++FF+LPL+EK  Y +LP   EGYGQ+
Sbjct: 58  MSKLHLACKEWGFFQLINHGAAEEVIEKMKADVQEFFKLPLKEKNAYAKLPXGVEGYGQN 117

Query: 578 FVKSDEQKLEWADMHLLITNPVKERNMRFWPNNPTSFRESVNKFSKDVQEVAMCLIGLMA 757
           FV S++QKL+WADMH L   P  ERNMRFWP  PTSFRE++ K+S ++ +V+ CL+ LMA
Sbjct: 118 FVVSZDQKLDWADMHFLQXLPASERNMRFWPEEPTSFRETLEKYSAELVKVSNCLLKLMA 177

Query: 758 RNLGLESQVLTKPFENCIQAVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXXXXXX 937
           +NL +  + LT  F++  QAVRMNYYPPC ++ KV+GLTPHSD                 
Sbjct: 178 KNLLINPEQLTNMFDDGRQAVRMNYYPPCVHASKVIGLTPHSDFGGLTLLVQVNEXQGLQ 237

Query: 938 IKKNGKWFPVQPILGAFV 991
           IK+NGKW P++P+ GAF+
Sbjct: 238 IKRNGKWIPIRPVPGAFI 255


>ref|XP_002279355.1| PREDICTED: S-norcoclaurine synthase 1 [Vitis vinifera]
           gi|297742171|emb|CBI33958.3| unnamed protein product
           [Vitis vinifera]
          Length = 353

 Score =  279 bits (713), Expect = 8e-73
 Identities = 132/259 (50%), Positives = 178/259 (68%)
 Frame = +2

Query: 215 GGSLPVDNVQALAGTELKDVPDRYVRSELEYDNDVVSTDNDVEIPVIDLSRLLNQKSASD 394
           G  LPV+NVQALA     ++P RY+R EL+  ++ V  D  ++IP ID+ +L+    A D
Sbjct: 11  GFFLPVENVQALASNNSGEIPSRYLRPELQ--SEEVLADESIQIPTIDMRKLM---VAED 65

Query: 395 ELAKFHSACLDWGFFQLINHGVSEEVIEKMKVDMEDFFRLPLEEKKVYGQLPDSTEGYGQ 574
           E+ K H AC +WGFFQLINHGV+EEVIEKMK D+++FF+LPL+EK  Y +LP+  EGYGQ
Sbjct: 66  EMGKLHYACKEWGFFQLINHGVAEEVIEKMKADLQEFFKLPLKEKNAYAKLPNGVEGYGQ 125

Query: 575 SFVKSDEQKLEWADMHLLITNPVKERNMRFWPNNPTSFRESVNKFSKDVQEVAMCLIGLM 754
            FV S +QKL+WAD+  L   P  ERNMRFWP  PTSFR + +K+S ++Q+V++CL+ LM
Sbjct: 126 HFVVSQDQKLDWADILFLQCRPASERNMRFWPQEPTSFRATFDKYSSELQKVSICLLELM 185

Query: 755 ARNLGLESQVLTKPFENCIQAVRMNYYPPCRYSDKVLGLTPHSDASSXXXXXXXXXXXXX 934
           A+NL ++   L   F+   Q +RMNYYPPC ++ KV+GLTPHSD                
Sbjct: 186 AKNLKVDPGQLMNMFQKGRQQIRMNYYPPCVHASKVIGLTPHSDICGLTLLAQVNEVQGL 245

Query: 935 XIKKNGKWFPVQPILGAFV 991
            IKKNGKW P++P+ GAF+
Sbjct: 246 QIKKNGKWIPIRPVPGAFI 264