BLASTX nr result
ID: Coptis24_contig00000287
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00000287
(2005 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta] 707 0.0
dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula] 490 e-174
gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago ... 489 e-174
ref|XP_003634774.1| PREDICTED: LOW QUALITY PROTEIN: secologanin ... 500 e-171
ref|XP_002265676.1| PREDICTED: secologanin synthase-like [Vitis ... 501 e-170
>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 518
Score = 707 bits (1825), Expect(2) = 0.0
Identities = 350/353 (99%), Positives = 352/353 (99%)
Frame = -1
Query: 1276 LKMMLPAIYTSCSEMVNKWEMMVSQDSCEMDVWPHLQNLTADVISRTAFGSSYEEGRQIF 1097
LKMMLPAIYTSCSEMVNKWEMMVSQDSCE+DVWPHLQNLTADVISRTAFGSSYEEGR+IF
Sbjct: 166 LKMMLPAIYTSCSEMVNKWEMMVSQDSCELDVWPHLQNLTADVISRTAFGSSYEEGRRIF 225
Query: 1096 QLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEA 917
QLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEA
Sbjct: 226 QLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEA 285
Query: 916 HNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLS 737
HNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLS
Sbjct: 286 HNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLS 345
Query: 736 MHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKL 557
MHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKL
Sbjct: 346 MHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKL 405
Query: 556 GEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRI 377
GEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRI
Sbjct: 406 GEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRI 465
Query: 376 CIGQNFALLESKMAIAMILLRFSFELSSTYVHAPYTVITLQPQHGAQLILHKL 218
CIGQNFALLESKMAIAMIL RFSFELSSTYVHAPYTVITLQPQHGAQLILHKL
Sbjct: 466 CIGQNFALLESKMAIAMILQRFSFELSSTYVHAPYTVITLQPQHGAQLILHKL 518
Score = 294 bits (753), Expect(2) = 0.0
Identities = 140/143 (97%), Positives = 142/143 (99%)
Frame = -3
Query: 1823 MVGGILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDM 1644
MVGGILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKD+
Sbjct: 1 MVGGILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDI 60
Query: 1643 VSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFTWYGPYCRVNIMDPDMIRDILS 1464
VSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSF WYGPYCRVNIMDPDMIRDILS
Sbjct: 61 VSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILS 120
Query: 1463 NKFGHFEKIRTNPLGKLLITGVV 1395
NKFGHFEKIRTNPLGKLLI+GVV
Sbjct: 121 NKFGHFEKIRTNPLGKLLISGVV 143
>dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]
Length = 518
Score = 490 bits (1262), Expect(2) = e-174
Identities = 237/354 (66%), Positives = 288/354 (81%), Gaps = 1/354 (0%)
Frame = -1
Query: 1276 LKMMLPAIYTSCSEMVNKWEMMVSQD-SCEMDVWPHLQNLTADVISRTAFGSSYEEGRQI 1100
LK+MLP + SC +M++KWE M+S D SCEMDVWP LQN +DVISR AFGSSYEEG++I
Sbjct: 163 LKIMLPIFHKSCDDMISKWEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGSSYEEGKRI 222
Query: 1099 FQLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGE 920
FQLQ+EQA L K + +YIPG RFLPT RMKEI + + L D++N RE+A+K GE
Sbjct: 223 FQLQTEQAELTTKIMMKVYIPGWRFLPTATPRRMKEIDRDIKASLTDMINNRERALKAGE 282
Query: 919 AHNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVL 740
A DDLLG+L+ESN KE+ ++ N+K+ GM++D+VI+ECKLFYFAGQETTS LLVWTMV+L
Sbjct: 283 ATKDDLLGILLESNHKEMEEHGNNKDVGMSLDDVIEECKLFYFAGQETTSVLLVWTMVLL 342
Query: 739 SMHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMK 560
S +PDWQ +AREEVLQVFG PDFDGL+HLKIVTMILYEVLRLYPP + +AR +K +K
Sbjct: 343 SRYPDWQARAREEVLQVFGNNKPDFDGLSHLKIVTMILYEVLRLYPPVIALARRVHKDVK 402
Query: 559 LGEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPR 380
LG + LPAGV + + ++VHHD ELWGEDA+ FNPERFS GV KAT + SFFPF+ GPR
Sbjct: 403 LGNLALPAGVQVFLSIIMVHHDIELWGEDAKVFNPERFSEGVLKATNGRNSFFPFAGGPR 462
Query: 379 ICIGQNFALLESKMAIAMILLRFSFELSSTYVHAPYTVITLQPQHGAQLILHKL 218
ICIGQNF++LE+KMAIAMIL RFSFELS +Y HAP TVITLQPQ+GA +ILHKL
Sbjct: 463 ICIGQNFSMLEAKMAIAMILQRFSFELSPSYAHAPATVITLQPQYGAHIILHKL 516
Score = 149 bits (376), Expect(2) = e-174
Identities = 74/139 (53%), Positives = 96/139 (69%)
Frame = -3
Query: 1811 ILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDMVSAT 1632
I+ L+T ++I WAW+ W+WL P+KLEK LREQG+KG SY+ L G++KD++
Sbjct: 7 IILLTLMTLSLI----WAWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDIKDLLKIR 62
Query: 1631 QEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFTWYGPYCRVNIMDPDMIRDILSNKFG 1452
+EA KPM LS IVPRV FVHQ+V K+GK SF W+GP RVNI DPD I+D+L NK
Sbjct: 63 KEATTKPMNLSDDIVPRVYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVL-NKIY 121
Query: 1451 HFEKIRTNPLGKLLITGVV 1395
F+K NPL +LL G+V
Sbjct: 122 DFQKPNANPLVRLLANGLV 140
>gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago truncatula]
Length = 518
Score = 489 bits (1259), Expect(2) = e-174
Identities = 236/354 (66%), Positives = 288/354 (81%), Gaps = 1/354 (0%)
Frame = -1
Query: 1276 LKMMLPAIYTSCSEMVNKWEMMVSQD-SCEMDVWPHLQNLTADVISRTAFGSSYEEGRQI 1100
LK+MLP + SC ++++KWE M+S D SCEMDVWP LQN +DVISR AFGSSYEEG++I
Sbjct: 163 LKIMLPIFHKSCDDLISKWEKMLSSDGSCEMDVWPFLQNFASDVISRAAFGSSYEEGKRI 222
Query: 1099 FQLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGE 920
FQLQ+EQA L K + +YIPG RFLPT RMKEI + + L D++N RE+A+K GE
Sbjct: 223 FQLQTEQAELTTKIMMKVYIPGWRFLPTATPRRMKEIDRDIKASLTDMINNRERALKAGE 282
Query: 919 AHNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVL 740
A DDLLG+L+ESN KE+ ++ N+K+ GM++D+VI+ECKLFYFAGQETTS LLVWTMV+L
Sbjct: 283 ATKDDLLGILLESNHKEMEEHGNNKDVGMSLDDVIEECKLFYFAGQETTSVLLVWTMVLL 342
Query: 739 SMHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMK 560
S +PDWQ +AREEVLQVFG PDFDGL+HLKIVTMILYEVLRLYPP + +AR +K +K
Sbjct: 343 SRYPDWQARAREEVLQVFGNNKPDFDGLSHLKIVTMILYEVLRLYPPVIALARRVHKDVK 402
Query: 559 LGEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPR 380
LG + LPAGV + + ++VHHD ELWGEDA+ FNPERFS GV KAT + SFFPF+ GPR
Sbjct: 403 LGNLALPAGVQVFLSIIMVHHDIELWGEDAKVFNPERFSEGVLKATNGRNSFFPFAGGPR 462
Query: 379 ICIGQNFALLESKMAIAMILLRFSFELSSTYVHAPYTVITLQPQHGAQLILHKL 218
ICIGQNF++LE+KMAIAMIL RFSFELS +Y HAP TVITLQPQ+GA +ILHKL
Sbjct: 463 ICIGQNFSMLEAKMAIAMILQRFSFELSPSYAHAPATVITLQPQYGAHIILHKL 516
Score = 149 bits (377), Expect(2) = e-174
Identities = 74/139 (53%), Positives = 95/139 (68%)
Frame = -3
Query: 1811 ILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDMVSAT 1632
ILFTL+ + WAW+ W+WL P+KLEK LREQG+KG SY+ L G++ D++
Sbjct: 8 ILFTLMTLSLI-----WAWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDMNDLLKMR 62
Query: 1631 QEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFTWYGPYCRVNIMDPDMIRDILSNKFG 1452
+EA +KPM LS IVPRV FVHQ+V K+GK SF W+GP RVNI DPD I+D+L NK
Sbjct: 63 KEATSKPMNLSDDIVPRVYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVL-NKIY 121
Query: 1451 HFEKIRTNPLGKLLITGVV 1395
F+K NPL +LL G+V
Sbjct: 122 DFQKPNANPLVRLLANGLV 140
>ref|XP_003634774.1| PREDICTED: LOW QUALITY PROTEIN: secologanin synthase-like [Vitis
vinifera]
Length = 516
Score = 500 bits (1288), Expect(2) = e-171
Identities = 238/353 (67%), Positives = 288/353 (81%)
Frame = -1
Query: 1276 LKMMLPAIYTSCSEMVNKWEMMVSQDSCEMDVWPHLQNLTADVISRTAFGSSYEEGRQIF 1097
LK MLPA + SCS+MV KW+M+ SCE+DVWP+L+NLT DVISRTAFGSSYEEGR+IF
Sbjct: 163 LKNMLPAFHLSCSDMVTKWKMLSVGGSCELDVWPYLENLTGDVISRTAFGSSYEEGRRIF 222
Query: 1096 QLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEA 917
QLQ EQ L I+ S+YIPG RFLPTK N RMK+I E LLR IVNKREKAMK GE
Sbjct: 223 QLQKEQTHLAIQVTMSVYIPGWRFLPTKTNRRMKQISKEVYALLRGIVNKREKAMKAGET 282
Query: 916 HNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLS 737
N DLLG+LMESNF+EI ++ N+K GM++ +VI+ECKLFY AGQETTS LLVWTMV+LS
Sbjct: 283 ANSDLLGILMESNFREIQEHQNNKKIGMSVRDVIEECKLFYLAGQETTSVLLVWTMVLLS 342
Query: 736 MHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKL 557
HP+WQ +AREEVLQVFG K P+ DGLNHLKIVTMI +EVLRLYPP ++AR YK ++
Sbjct: 343 EHPNWQARAREEVLQVFGNKKPEADGLNHLKIVTMIFHEVLRLYPPIAMLARAVYKDTQV 402
Query: 556 GEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRI 377
G++ PAGV + PT+LVHHD E+WG+DA++FNPERF+ GV KATKNQVSFFPF WGPR+
Sbjct: 403 GDMCFPAGVQV-XPTILVHHDHEIWGDDAKEFNPERFAEGVLKATKNQVSFFPFGWGPRV 461
Query: 376 CIGQNFALLESKMAIAMILLRFSFELSSTYVHAPYTVITLQPQHGAQLILHKL 218
CIGQNFA++E+K+A+AMIL FSFELS +Y HAP+ ++T+QPQ+GA LIL L
Sbjct: 462 CIGQNFAMMEAKIALAMILQHFSFELSPSYAHAPFNILTMQPQYGAHLILRGL 514
Score = 130 bits (328), Expect(2) = e-171
Identities = 64/136 (47%), Positives = 91/136 (66%)
Frame = -3
Query: 1802 TLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDMVSATQEA 1623
T+L++C W+ WVWL P++LE+CLREQG+ G SY+ L G+ K+M +EA
Sbjct: 14 TVLISCL--------WRLLNWVWLRPKRLERCLREQGLAGNSYRLLHGDFKEMSMMLKEA 65
Query: 1622 RAKPMELSHKIVPRVMPFVHQTVEKYGKLSFTWYGPYCRVNIMDPDMIRDILSNKFGHFE 1443
++P+ LS I PRV+PF ++KYGK F W+GP VNIM+P++IRDIL K F+
Sbjct: 66 YSRPISLSDDIAPRVLPFHCHFIKKYGKNFFAWFGPNPMVNIMEPELIRDILL-KSNVFQ 124
Query: 1442 KIRTNPLGKLLITGVV 1395
K +PLGKLL++G+V
Sbjct: 125 KPPPHPLGKLLVSGLV 140
>ref|XP_002265676.1| PREDICTED: secologanin synthase-like [Vitis vinifera]
Length = 516
Score = 501 bits (1289), Expect(2) = e-170
Identities = 235/353 (66%), Positives = 288/353 (81%)
Frame = -1
Query: 1276 LKMMLPAIYTSCSEMVNKWEMMVSQDSCEMDVWPHLQNLTADVISRTAFGSSYEEGRQIF 1097
LK MLPA + SCS+MV KW+M+ SCE+DVWP+L+NLT DVISRTAFGSSYEEGR+IF
Sbjct: 162 LKNMLPAFHLSCSDMVTKWKMLSVGGSCELDVWPYLENLTGDVISRTAFGSSYEEGRRIF 221
Query: 1096 QLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEA 917
QLQ EQ L I+ S+YIPG RFLPTK N RMK+I E LLR I+NKREKAMK GE
Sbjct: 222 QLQKEQTHLAIQVTMSVYIPGWRFLPTKTNRRMKQISKEVYALLRGIINKREKAMKAGET 281
Query: 916 HNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLS 737
N DLLG+LMESNF+EI ++ N+K M++ +VI+ECKLFY AGQETTS LLVWTMV+LS
Sbjct: 282 ANSDLLGILMESNFREIQEHQNNKKIRMSVKDVIEECKLFYLAGQETTSVLLVWTMVLLS 341
Query: 736 MHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKL 557
HP+WQ +AREEVLQVFG K P+ GLNHLKIVTMI +EVLRLYPP ++AR YK ++
Sbjct: 342 EHPNWQARAREEVLQVFGNKKPEAAGLNHLKIVTMIFHEVLRLYPPVAMLARAVYKDTQV 401
Query: 556 GEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRI 377
G++ PAGV + +PT+LVHHD E+WG+DA++FNPERF+ GV KATKNQVSFFPF WGPR+
Sbjct: 402 GDMCFPAGVQVVLPTILVHHDHEIWGDDAKEFNPERFAEGVLKATKNQVSFFPFGWGPRV 461
Query: 376 CIGQNFALLESKMAIAMILLRFSFELSSTYVHAPYTVITLQPQHGAQLILHKL 218
CIGQNFA++E+K+A+AMIL FSFELS +Y HAP++++T+QPQ+GA LIL L
Sbjct: 462 CIGQNFAMMEAKIALAMILQHFSFELSPSYAHAPFSILTMQPQYGAHLILRGL 514
Score = 125 bits (315), Expect(2) = e-170
Identities = 62/136 (45%), Positives = 91/136 (66%)
Frame = -3
Query: 1802 TLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDMVSATQEA 1623
T+L++C W+ WVWL P++LE+CLREQG+ G SY+ L G+ K+M +EA
Sbjct: 14 TVLISCL--------WRLLNWVWLRPKRLERCLREQGLAGNSYRLLHGDFKEMSMMLKEA 65
Query: 1622 RAKPMELSHKIVPRVMPFVHQTVEKYGKLSFTWYGPYCRVNIMDPDMIRDILSNKFGHFE 1443
++P+ LS +I PRV+PF ++KYG TW+GP VNIM+P++IRD+L K F+
Sbjct: 66 YSRPISLSDEIAPRVLPFHCHFIKKYG-TDMTWFGPNPMVNIMEPELIRDVLL-KSNVFQ 123
Query: 1442 KIRTNPLGKLLITGVV 1395
K +PLGKLL++G+V
Sbjct: 124 KPPPHPLGKLLVSGLV 139