BLASTX nr result
ID: Coptis24_contig00000068
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00000068
(2630 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta] 952 0.0
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 583 e-164
ref|XP_002282035.1| PREDICTED: cytochrome P450 82A3-like [Vitis ... 583 e-164
ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 583 e-163
ref|XP_002282085.2| PREDICTED: cytochrome P450 82A3-like [Vitis ... 568 e-159
>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 511
Score = 952 bits (2461), Expect = 0.0
Identities = 470/510 (92%), Positives = 477/510 (93%)
Frame = +2
Query: 230 FISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI 409
FISLYFLILWR SSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI
Sbjct: 2 FISLYFLILWRTRSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI 61
Query: 410 FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE 589
FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE
Sbjct: 62 FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE 121
Query: 590 LRKITMXXXXXXXXXXXXKHVRDSEINTSIQELYKVWKNHDKAKGPVLVDMKQWFGDLTL 769
LRKITM KHVRDSEI+ SIQ+LYKVWKNHDKAKGPVLVDMKQWFGDLTL
Sbjct: 122 LRKITMSELLSNRRLELLKHVRDSEIDASIQDLYKVWKNHDKAKGPVLVDMKQWFGDLTL 181
Query: 770 NVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWLDLQGYKK 949
NVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSW DLQGYKK
Sbjct: 182 NVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWFDLQGYKK 241
Query: 950 EMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSILEDTKISEYDNDTIIKST 1129
EMKNTAKELDSVFQRWLEEH RMRQTGELNREQDFMDVLMSILEDT+ISEYDNDTIIKST
Sbjct: 242 EMKNTAKELDSVFQRWLEEHNRMRQTGELNREQDFMDVLMSILEDTRISEYDNDTIIKST 301
Query: 1130 CLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXXQDELDSHVGKDRHVEESDIKNLIYLQA 1309
CLSIVTGG DTTMVTLTWI QDELDSHVGKDR VEESDIKNL+YLQA
Sbjct: 302 CLSIVTGGGDTTMVTLTWILSLLLNNKHALKKAQDELDSHVGKDRQVEESDIKNLVYLQA 361
Query: 1310 ITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPE 1489
ITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPE
Sbjct: 362 ITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPE 421
Query: 1490 RFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFLHEFESKTEMDMPVD 1669
RFLTSHVNMD+KGLHYELIPFGAGRR+CPGMSFTLQVVPLVLARFLHEF+SKTEMDMPVD
Sbjct: 422 RFLTSHVNMDVKGLHYELIPFGAGRRSCPGMSFTLQVVPLVLARFLHEFDSKTEMDMPVD 481
Query: 1670 MTETAGLTNAKATPLEVVITPRLHPEIYGL 1759
MTETAGLTNAKATPLEVVITPRLHPEIYGL
Sbjct: 482 MTETAGLTNAKATPLEVVITPRLHPEIYGL 511
>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|302142392|emb|CBI19595.3| unnamed protein product
[Vitis vinifera]
Length = 527
Score = 583 bits (1503), Expect = e-164
Identities = 289/526 (54%), Positives = 382/526 (72%), Gaps = 10/526 (1%)
Frame = +2
Query: 206 PMITLLVFFISL----YFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSE-LRH 370
P + L FF+ L F + R K++ ++APE GAWPIIGHLHLLGG + L +
Sbjct: 3 PALHLPAFFVFLSLIYVFYAMLGRKKIIKSSKSRDAPEPGGAWPIIGHLHLLGGGDQLLY 62
Query: 371 KTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYV 550
+TLGAMADKYGP F IR+G A VVS+ ++AKECFT NDKA A+RPT+ A+K +GY+Y
Sbjct: 63 RTLGAMADKYGPAFNIRLGSRRAFVVSSWEVAKECFTINDKALATRPTTVAAKHMGYNYA 122
Query: 551 MFGMAPYGQYWVELRKITMXXXXXXXXXXXXKHVRDSEINTSIQELYKVW-KNHDKAKGP 727
+FG APY +W E+RKI KHVR SE++ I+ELY +W KN + P
Sbjct: 123 VFGFAPYSPFWREMRKIATLELLSNRRLEILKHVRTSEVDMGIRELYGLWVKNSSR---P 179
Query: 728 VLVDMKQWFGDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDA 907
+LV++ +W D+TLNVI+RM+AGKRY G+ ++ D +EAR CQK + FFRL+G+F++ DA
Sbjct: 180 LLVELNRWLEDMTLNVIVRMVAGKRYFGAAAASDSSEARRCQKAISQFFRLIGIFVVSDA 239
Query: 908 LPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNRE--QDFMDVLMSILE 1081
LP+L WLDLQG+++ MK TAKELDS+ + WLEEH++ R + + E QDF+DV++S+ E
Sbjct: 240 LPFLWWLDLQGHERAMKTTAKELDSILEGWLEEHRQRRVSSLIKAEGEQDFIDVMLSLQE 299
Query: 1082 DTKIS--EYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXXQDELDSHVG 1255
+ ++S +YD++T IKSTCL+++ GGSDTT TLTW Q+ELD VG
Sbjct: 300 EGRLSGFQYDSETSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHALKKAQEELDLCVG 359
Query: 1256 KDRHVEESDIKNLIYLQAITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTY 1435
+R VEESD+KNL+YLQAI KE LRLYPAGPL GPR A DCTVAGY+VPAGTRLIVN +
Sbjct: 360 MERQVEESDVKNLVYLQAIIKETLRLYPAGPLLGPREALDDCTVAGYNVPAGTRLIVNIW 419
Query: 1436 KIQRDPLVWSEPSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVL 1615
K+QRDP VW+ P F+PERFL +H ++D+KG +EL+PFG+GRR+CPG+SF LQV+ L L
Sbjct: 420 KLQRDPSVWTNPCAFQPERFLNAHADVDVKGQQFELMPFGSGRRSCPGVSFALQVLHLTL 479
Query: 1616 ARFLHEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRLHPEIY 1753
AR LH FE T +D PVDMTE++GLT KATPLEV++TPRL+ ++Y
Sbjct: 480 ARLLHAFELSTPVDQPVDMTESSGLTIPKATPLEVLLTPRLNSKLY 525
>ref|XP_002282035.1| PREDICTED: cytochrome P450 82A3-like [Vitis vinifera]
Length = 528
Score = 583 bits (1503), Expect = e-164
Identities = 288/517 (55%), Positives = 365/517 (70%), Gaps = 1/517 (0%)
Frame = +2
Query: 191 QYLPTPMITLL-VFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELR 367
QYL I +L + F+S Y L+ RR S+ + APEA GAWPIIGHLHLLGGS+L
Sbjct: 11 QYLNITTIGVLGILFLSYYLLV--RR---SRAGKRRIAPEAAGAWPIIGHLHLLGGSQLP 65
Query: 368 HKTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDY 547
H TLG MADKYGPIF IR+GV+ ALVVS+ ++AKECFTTND A + RP A + LGY+Y
Sbjct: 66 HVTLGTMADKYGPIFTIRLGVHRALVVSSREVAKECFTTNDSAVSGRPKLVAPEHLGYNY 125
Query: 548 VMFGMAPYGQYWVELRKITMXXXXXXXXXXXXKHVRDSEINTSIQELYKVWKNHDKAKGP 727
MF +PY YW E+RKI K VR SE+ TSI+ELYK+W G
Sbjct: 126 AMFAFSPYDAYWREVRKIVNTELLSNRRLELLKDVRASEVETSIKELYKLWAEKKNELGH 185
Query: 728 VLVDMKQWFGDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDA 907
VLV+MKQWFGDL++NVILRM+ GKRY G + +E EAR CQK +R+FFRLLGLF+++D
Sbjct: 186 VLVEMKQWFGDLSMNVILRMVVGKRYFGVGAGGEEEEARRCQKAIREFFRLLGLFVVKDG 245
Query: 908 LPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSILEDT 1087
+P L WLDL G++K MK TAKE+DS+ Q WLEEH+R + GE N DFMDVL+S+L+
Sbjct: 246 IPSLGWLDLGGHEKAMKKTAKEIDSIAQEWLEEHRRRKDWGEDNGMHDFMDVLLSVLDGK 305
Query: 1088 KISEYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXXQDELDSHVGKDRH 1267
+ EYD DTI K+T +++++GG+DT VTLTW ++ELD+HVGK+R
Sbjct: 306 ALPEYDADTINKATSMALISGGTDTMTVTLTWALSLILNNRETLKKAKEELDTHVGKERL 365
Query: 1268 VEESDIKNLIYLQAITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQR 1447
V SDI L+YLQAI KE LRL P GPLSGPR DC + GYHVP GTRL++N K+ R
Sbjct: 366 VNASDISKLVYLQAIVKETLRLRPPGPLSGPRQFTEDCIIGGYHVPKGTRLVLNLSKLHR 425
Query: 1448 DPLVWSEPSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFL 1627
DP VW +P EF+PERFLT+H ++D +G H++L+PFGAGRR+CPG++F LQ++ L LA FL
Sbjct: 426 DPSVWLDPEEFQPERFLTTHRDVDARGQHFQLLPFGAGRRSCPGITFALQMLHLALASFL 485
Query: 1628 HEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRL 1738
H FE T + PVDM+E GLTN K+TPLE++I PRL
Sbjct: 486 HGFEVSTPSNAPVDMSEIPGLTNIKSTPLEILIAPRL 522
>ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|147794787|emb|CAN66846.1| hypothetical protein
VITISV_002367 [Vitis vinifera]
Length = 528
Score = 583 bits (1502), Expect = e-163
Identities = 287/515 (55%), Positives = 367/515 (71%), Gaps = 2/515 (0%)
Frame = +2
Query: 215 TLLVFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMAD 394
+LLV IS+ F L + ++S C AP A GAWPIIGHLHL G +L HKTLGAMAD
Sbjct: 14 SLLVVVISICFYRLKIK-AASNAKRCT-APRAGGAWPIIGHLHLFGAQQLTHKTLGAMAD 71
Query: 395 KYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYG 574
KYGP+F IR+G+N LV+S+S++A+ECFTT+D+ F++RP+ TASKILGYD+ MFG APYG
Sbjct: 72 KYGPVFTIRLGLNEILVLSSSEMARECFTTHDRVFSTRPSVTASKILGYDFAMFGFAPYG 131
Query: 575 QYWVELRKITMXXXXXXXXXXXXKHVRDSEINTSIQELYKVWKNHDKAKGPVLVDMKQWF 754
YW E+RKI KH+R SE+ TSI+ELY++W + G V VDMK+WF
Sbjct: 132 SYWREMRKIVTIELLSNHRLDMLKHIRASEVGTSIRELYEMWVSERGTDGRVFVDMKRWF 191
Query: 755 GDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWLDL 934
GDLTLN+ +R++ GKRY G+ + E E RTCQK +RDF L G+F++ DA+P+LSWLDL
Sbjct: 192 GDLTLNLAVRLVGGKRYFGAGADTKEGEGRTCQKVIRDFAHLFGVFVLSDAIPFLSWLDL 251
Query: 935 QGYKKEMKNTAKELDSVFQRWLEEHKRMRQT-GELNREQDFMDVLMSILEDTKISEYDND 1111
+GYKK MK TAKELDS+F WL+EHK R GE +QDFMDV++++LED S +D D
Sbjct: 252 KGYKKAMKRTAKELDSLFGGWLQEHKEKRLLGGEGKDDQDFMDVMLTVLEDVNFSGFDAD 311
Query: 1112 TIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXXQDELDSHVGKDRHVEESDIKN 1291
T+ K+TCL+++ GSDTT VTLTW Q ELD VGKDR V+ESD+KN
Sbjct: 312 TVNKATCLNLILAGSDTTKVTLTWALSLLLNHPHVLKKAQAELDIQVGKDRQVDESDVKN 371
Query: 1292 LIYLQAITKEALRLYPAGPLSGPRVADADCTV-AGYHVPAGTRLIVNTYKIQRDPLVWSE 1468
L+YLQAI KE LRLYPA P+ A DCT+ AGY++ AGT+++VN +KI RD VW
Sbjct: 372 LVYLQAIIKETLRLYPASPIITLHAAMEDCTLAAGYNISAGTQIMVNAWKIHRDERVWCN 431
Query: 1469 PSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFLHEFESKT 1648
P EF+PERF+TSH + D++G H+ELIPFG+GRR+CPG+S LQVV LA LH +E
Sbjct: 432 PKEFQPERFMTSHKDTDVRGQHFELIPFGSGRRSCPGISLALQVVHFALASLLHSYEVTK 491
Query: 1649 EMDMPVDMTETAGLTNAKATPLEVVITPRLHPEIY 1753
D VDMTE+ GLTN KATPLEV+++PRL E+Y
Sbjct: 492 PSDGDVDMTESLGLTNLKATPLEVLLSPRLKAELY 526
>ref|XP_002282085.2| PREDICTED: cytochrome P450 82A3-like [Vitis vinifera]
Length = 514
Score = 568 bits (1464), Expect = e-159
Identities = 278/511 (54%), Positives = 355/511 (69%), Gaps = 1/511 (0%)
Frame = +2
Query: 209 MITLLVFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSE-LRHKTLGA 385
M+ + Y L+LWR S + APEA GAWPIIGHLHLLGGS+ L H G
Sbjct: 1 MVGAFAILVLSYHLLLWR----SGAGKSRMAPEASGAWPIIGHLHLLGGSKNLPHLLFGT 56
Query: 386 MADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMA 565
MADKYGP+F IR+G+ A+VVS+ ++AKECFTT+D A ASRP A+K LGY+Y MF +
Sbjct: 57 MADKYGPVFSIRLGLKRAVVVSSWEMAKECFTTHDLALASRPELVAAKYLGYNYAMFAFS 116
Query: 566 PYGQYWVELRKITMXXXXXXXXXXXXKHVRDSEINTSIQELYKVWKNHDKAKGPVLVDMK 745
P+G YW E+RKI K+VR SE+ T ++ELYK+W G VLVDMK
Sbjct: 117 PHGAYWREVRKIATLELLSNRRLELLKNVRISEVETCMKELYKLWAEKKSEAGVVLVDMK 176
Query: 746 QWFGDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSW 925
QWFG L+LNVIL+M+ GKRY G + E EA+ CQK +R+FFRLLGLF++ DALP+L W
Sbjct: 177 QWFGHLSLNVILKMVVGKRYFGYAAESKEKEAQQCQKAIREFFRLLGLFVVSDALPFLGW 236
Query: 926 LDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSILEDTKISEYD 1105
LD+ G+ K MK TAKELD + Q WLEEH+R + +GE + +QDFMDV++SIL T + YD
Sbjct: 237 LDVGGHVKAMKKTAKELDGIAQEWLEEHRRRKDSGEADGDQDFMDVMLSILGATDPNGYD 296
Query: 1106 NDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXXQDELDSHVGKDRHVEESDI 1285
DTI K+T L ++ GG+DTT VTLTW Q+ELD+HVGK+R V E DI
Sbjct: 297 ADTINKATSLILIAGGTDTTSVTLTWAISLLLNNPHVLRKAQEELDTHVGKERLVNEMDI 356
Query: 1286 KNLIYLQAITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWS 1465
L+YLQAI KE LRLYPA PLSG R D + GYH+P GTRL++N KIQRDP VW
Sbjct: 357 SKLVYLQAIVKETLRLYPAAPLSGQRQFIQDSVLGGYHIPKGTRLLLNLTKIQRDPRVWL 416
Query: 1466 EPSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFLHEFESK 1645
P++F+P RFLT++ ++D+KG H+ L PFG GRR CPG +F LQV+PL LA FLH+F+
Sbjct: 417 NPTKFQPSRFLTTYKDVDVKGKHFVLTPFGGGRRICPGAAFALQVLPLTLANFLHKFQLS 476
Query: 1646 TEMDMPVDMTETAGLTNAKATPLEVVITPRL 1738
T + P+DM+E+ G+TN K+TPLEV+I+PRL
Sbjct: 477 TPSNSPIDMSESFGITNIKSTPLEVLISPRL 507