BLASTX nr result
ID: Coptis23_contig00035378
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00035378
(542 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||S65812 RNA-directed DNA polymerase (EC 2.7.7.49) (clone DW1... 50 5e-12
gb|AAF97969.1|AC000103_19 F21J9.30 [Arabidopsis thaliana] 46 8e-12
gb|AAG03119.1|AC004133_13 F5A9.24 [Arabidopsis thaliana] 46 8e-12
gb|AAD29058.1| putative non-LTR retroelement reverse transcripta... 65 3e-11
gb|AFP55574.1| non-ltr retroelement reverse transcriptase [Rosa ... 52 8e-11
>pir||S65812 RNA-directed DNA polymerase (EC 2.7.7.49) (clone DW15) -
Arabidopsis thaliana retrotransposon Ta11-1
gi|976278|gb|AAA75254.1| reverse transcriptase
[Arabidopsis thaliana]
Length = 1333
Score = 50.4 bits (119), Expect(3) = 5e-12
Identities = 30/88 (34%), Positives = 46/88 (52%), Gaps = 4/88 (4%)
Frame = -3
Query: 375 DTEDKWQQEEETISKVFVEHFQRNFESSDDDSTSQIFNSCTPRVT-ALNNKI---PKEDE 208
D + ++E + +F F+S+D S +F PRVT ++NN + ++E
Sbjct: 375 DVNGLFHKDEMNKGAIAEAYFSDLFKSTDPSSFVDLFEDYQPRVTESMNNTLIAAVSKNE 434
Query: 207 IRVAAF*IVANKSVGPDGFTGKFFHTYW 124
IR A F I ++ + G DGFTG FF YW
Sbjct: 435 IREAVFAIRSSSAPGVDGFTGFFFQKYW 462
Score = 34.3 bits (77), Expect(3) = 5e-12
Identities = 16/34 (47%), Positives = 18/34 (52%)
Frame = -1
Query: 500 EESHWAQIANTTWHQEGDDNTKFFPLSVIINRQK 399
EE W+Q + W GD NT FF SV NR K
Sbjct: 334 EEVFWSQKSRAKWMHSGDKNTSFFHASVKDNRGK 367
Score = 30.4 bits (67), Expect(3) = 5e-12
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -2
Query: 106 MTNFIQQFYFNAHFTKPLNHINIVLIPKTKKP 11
+T IQ F+ +F K N ++ L+PK KKP
Sbjct: 469 VTKEIQNFFLLGYFPKSWNFTHLCLLPKKKKP 500
>gb|AAF97969.1|AC000103_19 F21J9.30 [Arabidopsis thaliana]
Length = 1270
Score = 45.8 bits (107), Expect(3) = 8e-12
Identities = 28/93 (30%), Positives = 41/93 (44%), Gaps = 4/93 (4%)
Frame = -3
Query: 390 IATIKDTEDKWQQEEETISKVFVEHFQRNFESSDDDSTSQIFNSCTPRVTALNNKI---- 223
I +KD Q E +V +F F+SS+ + F+ PRV+ + N+
Sbjct: 331 IEKLKDVNGNMQTSEAAKGEVAAAYFGNLFKSSNPSGFTDWFSGLVPRVSEVMNESLVGE 390
Query: 222 PKEDEIRVAAF*IVANKSVGPDGFTGKFFHTYW 124
EI+ A F I + GPDG + FF YW
Sbjct: 391 VSAQEIKEAVFSIKPASAPGPDGMSALFFQHYW 423
Score = 37.0 bits (84), Expect(3) = 8e-12
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = -1
Query: 533 LTKLLANLVLHEESHWAQIANTTWHQEGDDNTKFFPLSVIINRQKLKV 390
L K LA EE++W Q + W + G+ N+K+F +V NRQ+ ++
Sbjct: 284 LKKDLAKAYREEEAYWKQKSRQKWLRSGNRNSKYFHAAVKQNRQRKRI 331
Score = 31.6 bits (70), Expect(3) = 8e-12
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = -2
Query: 139 FSYLLDTVGGDMTNFIQQFYFNAHFTKPLNHINIVLIPKTKKP 11
F + TVG +T+ +++F+ + N+ ++ LIPKT+ P
Sbjct: 419 FQHYWSTVGNQVTSEVKKFFADGIMPAEWNYTHLCLIPKTQHP 461
>gb|AAG03119.1|AC004133_13 F5A9.24 [Arabidopsis thaliana]
Length = 1254
Score = 45.8 bits (107), Expect(3) = 8e-12
Identities = 28/93 (30%), Positives = 41/93 (44%), Gaps = 4/93 (4%)
Frame = -3
Query: 390 IATIKDTEDKWQQEEETISKVFVEHFQRNFESSDDDSTSQIFNSCTPRVTALNNKI---- 223
I +KD Q E +V +F F+SS+ + F+ PRV+ + N+
Sbjct: 334 IEKLKDVNGNMQTSEAAKGEVAAAYFGNLFKSSNPSGFTDWFSGLVPRVSEVMNESLVGE 393
Query: 222 PKEDEIRVAAF*IVANKSVGPDGFTGKFFHTYW 124
EI+ A F I + GPDG + FF YW
Sbjct: 394 VSAQEIKEAVFSIKPASAPGPDGMSALFFQHYW 426
Score = 37.0 bits (84), Expect(3) = 8e-12
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = -1
Query: 533 LTKLLANLVLHEESHWAQIANTTWHQEGDDNTKFFPLSVIINRQKLKV 390
L K LA EE++W Q + W + G+ N+K+F +V NRQ+ ++
Sbjct: 287 LKKDLAKAYREEEAYWKQKSRQKWLRSGNRNSKYFHAAVKQNRQRKRI 334
Score = 31.6 bits (70), Expect(3) = 8e-12
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = -2
Query: 139 FSYLLDTVGGDMTNFIQQFYFNAHFTKPLNHINIVLIPKTKKP 11
F + TVG +T+ +++F+ + N+ ++ LIPKT+ P
Sbjct: 422 FQHYWSTVGNQVTSEVKKFFADGIMPAEWNYTHLCLIPKTQHP 464
>gb|AAD29058.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana]
Length = 1229
Score = 65.1 bits (157), Expect(2) = 3e-11
Identities = 33/93 (35%), Positives = 53/93 (56%), Gaps = 4/93 (4%)
Frame = -3
Query: 390 IATIKDTEDKWQQEEETISKVFVEHFQRNFESSDDDSTSQIFNSCTPRVTALNN----KI 223
+ ++D Q EE IS++ +FQ+ F S D S + + P V+ +N +I
Sbjct: 263 LTVMEDINGVAQHEEHQISQIISGYFQQIFTSESDGDFSVVDEAIEPMVSQGDNDFLTRI 322
Query: 222 PKEDEIRVAAF*IVANKSVGPDGFTGKFFHTYW 124
P ++E++ A F I A+K+ GPDGFT F+H+YW
Sbjct: 323 PNDEEVKDAVFSINASKAPGPDGFTAGFYHSYW 355
Score = 28.1 bits (61), Expect(2) = 3e-11
Identities = 13/35 (37%), Positives = 16/35 (45%)
Frame = -1
Query: 533 LTKLLANLVLHEESHWAQIANTTWHQEGDDNTKFF 429
LT L + EE W Q + W GD NT +F
Sbjct: 216 LTATLEHAYKLEEQFWKQRSRVLWLHSGDRNTGYF 250
>gb|AFP55574.1| non-ltr retroelement reverse transcriptase [Rosa rugosa]
Length = 1656
Score = 52.0 bits (123), Expect(3) = 8e-11
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 4/93 (4%)
Frame = -3
Query: 390 IATIKDTEDKWQQEEETISKVFVEHFQRNFESSDDDSTSQIFNSCTPRVTALNNKIPKED 211
I +KD W E ++ F+++F ++S+ ++ + VTA NKI
Sbjct: 977 IVRLKDDHGNWLDSEADVALQFLDYFTALYQSNGPQQWEEVLDFVDTAVTAEMNKILSSP 1036
Query: 210 ----EIRVAAF*IVANKSVGPDGFTGKFFHTYW 124
E++ A F + A K+ GPDGF+G F+ W
Sbjct: 1037 VSLLEVKKAVFDLGATKAPGPDGFSGIFYQNQW 1069
Score = 33.5 bits (75), Expect(3) = 8e-11
Identities = 16/48 (33%), Positives = 24/48 (50%)
Frame = -1
Query: 533 LTKLLANLVLHEESHWAQIANTTWHQEGDDNTKFFPLSVIINRQKLKV 390
LT + L +E +W Q + W + GD N+ FF + I RQ K+
Sbjct: 930 LTDQVTKLWTQDEMYWHQRSRVNWLKLGDQNSSFFHQTTIQRRQYNKI 977
Score = 25.4 bits (54), Expect(3) = 8e-11
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2
Query: 55 LNHINIVLIPKTKKPGHP 2
+N ++ LIPK K P HP
Sbjct: 1093 MNRTHLALIPKVKAPTHP 1110