BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00034027
         (698 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003518515.1| PREDICTED: taxadiene 5-alpha hydroxylase-lik...   290   1e-76
ref|XP_002520384.1| cytochrome P450, putative [Ricinus communis]...   290   1e-76
ref|XP_002302147.1| cytochrome P450 [Populus trichocarpa] gi|222...   283   3e-74
ref|NP_181813.1| cytochrome P450, family 718 [Arabidopsis thalia...   275   6e-72
ref|XP_003617455.1| Taxane 13-alpha-hydroxylase [Medicago trunca...   275   8e-72

>ref|XP_003518515.1| PREDICTED: taxadiene 5-alpha hydroxylase-like [Glycine max]
          Length = 478

 Score =  290 bits (743), Expect = 1e-76
 Identities = 146/232 (62%), Positives = 186/232 (80%)
 Frame = -1

Query: 698 ETYEFYKAQRNNKLFEEFVEPRMKRYGNIFKTRLMGSSTVVVNGASANKFFLSDEFKLVV 519
           ET EF+ AQR N+LFEEFV PR+ ++G IF+TR+MGS TVVVNGA ANKF LS+EFKLV 
Sbjct: 50  ETMEFFNAQRRNQLFEEFVHPRILKHGRIFRTRIMGSPTVVVNGAEANKFLLSNEFKLVK 109

Query: 518 SSWPSASVQLMGPTSIM*KQGDQHRCIRGLIAASLISCTSLESIVPKICRSVELHFNAHW 339
           SSWPS+SV+LMG  SIM K G +HR +RG+I  SL     LE +VPK+C SV+ H   +W
Sbjct: 110 SSWPSSSVELMGRDSIMEKDGGRHRFLRGVIGTSL-GYAGLELLVPKLCNSVQFHLATNW 168

Query: 338 QGEDSISLFRSTKMLTFSIVFKCLLGIKVESGMLEMFENVLEGVFAVPVEFPGTKFWHAK 159
           +G++ ISL+RSTK+L+FSIVF+CLLGIKVE GML+ FE VLEGVF+  V FPG+KFW AK
Sbjct: 169 KGQEKISLYRSTKVLSFSIVFECLLGIKVEPGMLDTFERVLEGVFSPAVMFPGSKFWRAK 228

Query: 158 RARLGIEEVLLSVIRKKRNELEESYGRENGDGLLLSRLVASLIRGEISEAEV 3
           +AR+ IE++L+ V+R+KR E+E S GRE  DG+LLS+LV+ +I+GEISE EV
Sbjct: 229 KARVEIEKMLVKVVREKRREMEGSLGREQ-DGMLLSKLVSGMIQGEISEKEV 279


>ref|XP_002520384.1| cytochrome P450, putative [Ricinus communis]
           gi|223540431|gb|EEF42000.1| cytochrome P450, putative
           [Ricinus communis]
          Length = 379

 Score =  290 bits (743), Expect = 1e-76
 Identities = 142/232 (61%), Positives = 188/232 (81%)
 Frame = -1

Query: 698 ETYEFYKAQRNNKLFEEFVEPRMKRYGNIFKTRLMGSSTVVVNGASANKFFLSDEFKLVV 519
           ET EF++AQR+N+LFEEFV+PR+ +YGNIFKTRLMGS T+VVNGA AN+FFLS+EFKLV+
Sbjct: 53  ETMEFFQAQRSNRLFEEFVQPRIAKYGNIFKTRLMGSPTIVVNGAEANRFFLSNEFKLVI 112

Query: 518 SSWPSASVQLMGPTSIM*KQGDQHRCIRGLIAASLISCTSLESIVPKICRSVELHFNAHW 339
           SSWP+++VQLMG  SIM KQGDQHRCIRGLIA +L S   LE +VPK+C+SVE+H   +W
Sbjct: 113 SSWPTSTVQLMGKNSIMEKQGDQHRCIRGLIATTL-SNARLEVLVPKMCQSVEVHLEKYW 171

Query: 338 QGEDSISLFRSTKMLTFSIVFKCLLGIKVESGMLEMFENVLEGVFAVPVEFPGTKFWHAK 159
            G+ ++SL+RS K LTF+IVF+CLLGIKV++G L  FE +L+GVFA P   PG++F  AK
Sbjct: 172 NGDKNVSLYRSAKALTFTIVFECLLGIKVDAGTLSTFEWILDGVFATPFSLPGSRFSKAK 231

Query: 158 RARLGIEEVLLSVIRKKRNELEESYGRENGDGLLLSRLVASLIRGEISEAEV 3
           +AR  +E++L+ ++R+KR E+EE   R + DG+LL +L+  +IRGEI E EV
Sbjct: 232 KARKEVEKMLVELVREKRKEMEEGLQRVD-DGVLLCQLIGGMIRGEIREEEV 282


>ref|XP_002302147.1| cytochrome P450 [Populus trichocarpa] gi|222843873|gb|EEE81420.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 459

 Score =  283 bits (723), Expect = 3e-74
 Identities = 141/232 (60%), Positives = 183/232 (78%)
 Frame = -1

Query: 698 ETYEFYKAQRNNKLFEEFVEPRMKRYGNIFKTRLMGSSTVVVNGASANKFFLSDEFKLVV 519
           ET EFY++QR+N+LFEEFV+PR+ +YG IFKTRLMGS T++VNGA AN+FFLS+EFKLVV
Sbjct: 32  ETIEFYRSQRDNQLFEEFVQPRITKYGKIFKTRLMGSPTIIVNGAEANRFFLSNEFKLVV 91

Query: 518 SSWPSASVQLMGPTSIM*KQGDQHRCIRGLIAASLISCTSLESIVPKICRSVELHFNAHW 339
           SSWP+ASVQLMG  SIM KQG++HRC+RG+IA SL     LE +VPKIC SV+L+ + +W
Sbjct: 92  SSWPTASVQLMGINSIMEKQGEKHRCLRGIIATSL-GPAGLEILVPKICDSVQLYLDKNW 150

Query: 338 QGEDSISLFRSTKMLTFSIVFKCLLGIKVESGMLEMFENVLEGVFAVPVEFPGTKFWHAK 159
              + ISL+ STK LTF+IV +CLLG+  E G L  FE VLEGVFA P+ FPG+KF  AK
Sbjct: 151 NVREEISLYHSTKALTFTIVLECLLGLNFEPGTLNTFERVLEGVFAPPISFPGSKFSRAK 210

Query: 158 RARLGIEEVLLSVIRKKRNELEESYGRENGDGLLLSRLVASLIRGEISEAEV 3
           +AR  I+E+L+ V+R+KR ++E   G +  +G+L S+LV+ +IRGEISE EV
Sbjct: 211 KARREIKEMLIKVVREKRKKMESGLGGD--EGMLFSQLVSGMIRGEISEEEV 260


>ref|NP_181813.1| cytochrome P450, family 718 [Arabidopsis thaliana]
           gi|4512670|gb|AAD21724.1| putative cytochrome P450
           [Arabidopsis thaliana] gi|111074222|gb|ABH04484.1|
           At2g42850 [Arabidopsis thaliana]
           gi|330255083|gb|AEC10177.1| cytochrome P450, family 718
           [Arabidopsis thaliana]
          Length = 485

 Score =  275 bits (703), Expect = 6e-72
 Identities = 138/232 (59%), Positives = 182/232 (78%)
 Frame = -1

Query: 698 ETYEFYKAQRNNKLFEEFVEPRMKRYGNIFKTRLMGSSTVVVNGASANKFFLSDEFKLVV 519
           ET +FYKAQ++N++FE+FV PR+ ++GNIFKTR+MGS T+VVNGA AN+  LS+EF LVV
Sbjct: 57  ETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVV 116

Query: 518 SSWPSASVQLMGPTSIM*KQGDQHRCIRGLIAASLISCTSLESIVPKICRSVELHFNAHW 339
           SSWPS+SVQLMG   IM KQG++HR +RG++A SL S   LES++PK+C +V+ H    W
Sbjct: 117 SSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSL-SYIGLESLIPKLCDTVKFHHETEW 175

Query: 338 QGEDSISLFRSTKMLTFSIVFKCLLGIKVESGMLEMFENVLEGVFAVPVEFPGTKFWHAK 159
           +G++ ISL+RS K+LTF++VF+CL GIKVE GMLE+FE VLEGVFA+PVEFP +KF  AK
Sbjct: 176 RGKEEISLYRSAKVLTFTVVFECLYGIKVEIGMLEVFERVLEGVFALPVEFPCSKFARAK 235

Query: 158 RARLGIEEVLLSVIRKKRNELEESYGRENGDGLLLSRLVASLIRGEISEAEV 3
           +ARL IE  L+  +R+KR E+E+  G E  +  L SRLV  LI+G I+E EV
Sbjct: 236 KARLEIETFLVGKVREKRREMEKE-GAEKPNTTLFSRLVEELIKGVITEEEV 286


>ref|XP_003617455.1| Taxane 13-alpha-hydroxylase [Medicago truncatula]
           gi|355518790|gb|AET00414.1| Taxane 13-alpha-hydroxylase
           [Medicago truncatula]
          Length = 479

 Score =  275 bits (702), Expect = 8e-72
 Identities = 132/232 (56%), Positives = 181/232 (78%)
 Frame = -1

Query: 698 ETYEFYKAQRNNKLFEEFVEPRMKRYGNIFKTRLMGSSTVVVNGASANKFFLSDEFKLVV 519
           ET EF+ AQR NKL+E+FV PR+ ++G IFKTR++GS TV+VNGA ANKF LS+EFKLV 
Sbjct: 50  ETMEFFNAQRRNKLYEDFVHPRITKHGKIFKTRIIGSPTVIVNGAEANKFILSNEFKLVK 109

Query: 518 SSWPSASVQLMGPTSIM*KQGDQHRCIRGLIAASLISCTSLESIVPKICRSVELHFNAHW 339
           SSWPS+SV LMG  SIM K G++HR +RG+I  S      LE++VPK+C  V+L+ + +W
Sbjct: 110 SSWPSSSVHLMGKDSIMEKDGERHRFLRGVIGTSF-GYAGLETLVPKLCNFVKLYLSKNW 168

Query: 338 QGEDSISLFRSTKMLTFSIVFKCLLGIKVESGMLEMFENVLEGVFAVPVEFPGTKFWHAK 159
           QG++ ISL+RSTK+LTFSIVF+CLLGI VE GM++ FE VLEGVF+  ++FPG+KFW A 
Sbjct: 169 QGQEEISLYRSTKVLTFSIVFECLLGINVEPGMVDTFERVLEGVFSPAIKFPGSKFWRAM 228

Query: 158 RARLGIEEVLLSVIRKKRNELEESYGRENGDGLLLSRLVASLIRGEISEAEV 3
           +AR  IE++++ V+R+KR E+EE   +   D +L+S+LV  +I+GEI+E E+
Sbjct: 229 KARKEIEKMIVKVVREKRKEIEEGKLKREEDRMLMSKLVYGMIQGEITEKEI 280