BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00030462
         (393 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      256   1e-66
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   248   5e-64
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       228   4e-58
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   205   3e-51
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   196   1e-48

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  256 bits (654), Expect = 1e-66
 Identities = 115/130 (88%), Positives = 124/130 (95%)
 Frame = -2

Query: 392 EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 213
           EHMKNYELLLRKISEW+  DGLLF+EH+CHKTFAYHYEPLD+DDWFTEYVFPAGT+IIPS
Sbjct: 207 EHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIPS 266

Query: 212 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 33
           ASF LYFQDDVSVVNHWTLSGKH+SRTNEEWLKRLDAN+D IKP+FETL G+ EEAVKLI
Sbjct: 267 ASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKLI 326

Query: 32  NYWRGFCLSG 3
           NYWRGFCLSG
Sbjct: 327 NYWRGFCLSG 336


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  248 bits (632), Expect = 5e-64
 Identities = 111/131 (84%), Positives = 122/131 (93%), Gaps = 1/131 (0%)
 Frame = -2

Query: 392 EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 213
           EHMKNYELLLRKI EWM  DGLLF+EH+CHKTFAYHYEP+DEDDWFTEYVFPAGT+IIPS
Sbjct: 209 EHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFTEYVFPAGTMIIPS 268

Query: 212 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSA-EEAVKL 36
           ASF LYFQDDVSVVNHWTLSGKH+SRTNEEWLKRLDAN++ IKP+F T+TG   +EA+KL
Sbjct: 269 ASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFVTITGQCRQEAMKL 328

Query: 35  INYWRGFCLSG 3
           INYWRGFCLSG
Sbjct: 329 INYWRGFCLSG 339


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  228 bits (581), Expect = 4e-58
 Identities = 101/130 (77%), Positives = 113/130 (86%)
 Frame = -2

Query: 392 EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 213
           EHMKNY LLL+KISEWM  DGLLF++HVCHKTFA+H EP+DEDDW +EY FPAGT+IIPS
Sbjct: 200 EHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSEYAFPAGTMIIPS 259

Query: 212 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 33
           ASF LYFQDD SVVNHWTLSGKH+SRT EEWLKRLDAN+  I P+F T+TGS EEAVKL+
Sbjct: 260 ASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFATITGSEEEAVKLL 319

Query: 32  NYWRGFCLSG 3
           NYWRG C  G
Sbjct: 320 NYWRGLCFFG 329


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
           somniferum]
          Length = 351

 Score =  205 bits (522), Expect = 3e-51
 Identities = 89/130 (68%), Positives = 109/130 (83%)
 Frame = -2

Query: 392 EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 213
           EHMKNY+LLL+KISEWM  DGLLF+EHVCHKT AYHYEP+D +DW+T Y+FPAGT+ + S
Sbjct: 200 EHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYIFPAGTLTLSS 259

Query: 212 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 33
           AS LLYFQDDVSVVN WTLSGKHYSR++EEWLK +D NI   K I  ++T + +EA+KL+
Sbjct: 260 ASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRSITKTEKEAIKLL 319

Query: 32  NYWRGFCLSG 3
           N+WR FC+ G
Sbjct: 320 NFWRIFCMCG 329


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
           gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
           [Thalictrum flavum]
          Length = 356

 Score =  196 bits (499), Expect = 1e-48
 Identities = 86/129 (66%), Positives = 106/129 (82%)
 Frame = -2

Query: 392 EHMKNYELLLRKISEWMIPDGLLFIEHVCHKTFAYHYEPLDEDDWFTEYVFPAGTVIIPS 213
           EHMKNYEL L+K+S W+   GLLF+EH CHK FAY YEPLDEDDW+TEY+FP+GT+++ S
Sbjct: 205 EHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDEDDWYTEYIFPSGTLVMSS 264

Query: 212 ASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDANIDAIKPIFETLTGSAEEAVKLI 33
           +S LLYFQ+DVSVVNHWTLSGKH S   ++WLKRLD NID +K IFE+  GS E+A+K I
Sbjct: 265 SSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIFESFYGSKEKAMKFI 324

Query: 32  NYWRGFCLS 6
            YWR FC++
Sbjct: 325 TYWRVFCIA 333