BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00026957
         (412 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      160   1e-37
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   149   2e-34
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       141   6e-32
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   112   3e-23
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   104   6e-21

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  160 bits (404), Expect = 1e-37
 Identities = 77/104 (74%), Positives = 89/104 (85%)
 Frame = +1

Query: 100 MALENKQPKKAAVMELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKF 279
           MA+E KQ KKAA++ELLK+LELGLVP D++KQL+R ELARRLQWGYKPT E+Q+AE+   
Sbjct: 1   MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNL 60

Query: 280 AHSLRHMNIAMEVHTLDSQMYEVPIEFLKIMFGSTLKGSCCYFK 411
            HSLR M IA EV TLDSQ+YE+PIEFLKIM GS LKGSCCYFK
Sbjct: 61  THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFK 104


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  149 bits (376), Expect = 2e-34
 Identities = 74/106 (69%), Positives = 87/106 (82%), Gaps = 2/106 (1%)
 Frame = +1

Query: 100 MALENKQ--PKKAAVMELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVL 273
           MA+E KQ  PKKA ++ELLKKLELGLVP DE+K+L+RI+L RRLQWG K T E+Q+A+++
Sbjct: 1   MAVEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLV 60

Query: 274 KFAHSLRHMNIAMEVHTLDSQMYEVPIEFLKIMFGSTLKGSCCYFK 411
              HSLR M IA EV TLD QMYEVPI+FLKIM GS LKGSCCYFK
Sbjct: 61  NLTHSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFK 106


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  141 bits (355), Expect = 6e-32
 Identities = 69/90 (76%), Positives = 77/90 (85%)
 Frame = +1

Query: 142 ELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKFAHSLRHMNIAMEVH 321
           EL KKLELGL+P DEVK+LMRI+LARRLQWGYK T E+Q+A+VL   HSLRHMNIAMEV 
Sbjct: 8   ELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVD 67

Query: 322 TLDSQMYEVPIEFLKIMFGSTLKGSCCYFK 411
           TLDS MYEVP++FLKIM GS LK S CYFK
Sbjct: 68  TLDSNMYEVPLDFLKIMNGSALKMSSCYFK 97


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
           gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
           [Thalictrum flavum]
          Length = 356

 Score =  112 bits (280), Expect = 3e-23
 Identities = 53/102 (51%), Positives = 78/102 (76%)
 Frame = +1

Query: 106 LENKQPKKAAVMELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKFAH 285
           +E KQ KK AV  L+K++E G V  +E++ +M+I++ +RL+WGYKPT+EQQ+A+++ FA 
Sbjct: 1   METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60

Query: 286 SLRHMNIAMEVHTLDSQMYEVPIEFLKIMFGSTLKGSCCYFK 411
           SL+ M +A EV TLD+++YE+P+ FL IM G TLK S  YFK
Sbjct: 61  SLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFK 102


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
           somniferum]
          Length = 351

 Score =  104 bits (260), Expect = 6e-21
 Identities = 46/89 (51%), Positives = 68/89 (76%)
 Frame = +1

Query: 142 ELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKFAHSLRHMNIAMEVH 321
           ELL+ +ELGL+P  E++QL+R+EL +RLQWGYK T+E+Q++++L   HSL+ M +A E+ 
Sbjct: 8   ELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEME 67

Query: 322 TLDSQMYEVPIEFLKIMFGSTLKGSCCYF 408
            LD ++YE P+EFLKI  GS +K S  Y+
Sbjct: 68  NLDLKLYEAPMEFLKIQHGSNMKQSAGYY 96