BLASTX nr result
ID: Coptis23_contig00024312
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00024312
(655 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptas... 80 4e-21
gb|ABD28670.2| RNA-directed DNA polymerase (Reverse transcriptas... 65 3e-20
emb|CAN74312.1| hypothetical protein VITISV_037520 [Vitis vinifera] 73 8e-18
ref|XP_002450418.1| hypothetical protein SORBIDRAFT_05g005061 [S... 60 9e-18
pir||S65812 RNA-directed DNA polymerase (EC 2.7.7.49) (clone DW1... 61 2e-17
>gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptase); Ribonuclease
H; Endonuclease/exonuclease/phosphatase [Medicago
truncatula]
Length = 1246
Score = 80.5 bits (197), Expect(2) = 4e-21
Identities = 39/99 (39%), Positives = 62/99 (62%), Gaps = 1/99 (1%)
Frame = -3
Query: 296 ATLADQKEIGEHIVQ*FTKKLKRVELQLDDDLI-NLMPKSVT*EDNENLIKMPPLEEIKK 120
A + D I H++ F + +DL+ + +P V+ DN +L+++P E+K
Sbjct: 390 AVITDPARIEVHVLNYFQAIFSVDNSCIQNDLVVDTIPSLVSNVDNNSLLRLPLWGEVKN 449
Query: 119 SVFDLNPDSSPGSDGFTGHFFQTYWDIVGSDMVRAVQYF 3
+VF LN D +PG +GF GHF+QTYWDIVG+D++++VQ F
Sbjct: 450 AVFTLNGDGAPGPNGFGGHFYQTYWDIVGADVIQSVQDF 488
Score = 46.6 bits (109), Expect(2) = 4e-21
Identities = 25/115 (21%), Positives = 59/115 (51%), Gaps = 1/115 (0%)
Frame = -1
Query: 643 GHSIFVLAQKLKRLKGALKSWHRNVFGNLGTQVRDEFAALESLKKDLER-NYNDVLANEI 467
GH + L KLK +K + W+R VFG++ +VR + +++ ++ ++D L +
Sbjct: 273 GHGMTRLQAKLKHMKQVFRHWNRTVFGDVDRKVRMAVEEVNRIQQIIDSVGFSDQLYAQE 332
Query: 466 INQENKVEGLLEQEESFWPQKSKVKWDNELDKSTKFFHALADMNRNKSYITEMEN 302
+ + L ++ W +K + + D++T +FH ++ + K+ I+ +++
Sbjct: 333 LEAHLILTKALHYQDELWREKLRDQRFIHGDRNTAYFHRISKVRATKNTISFLQD 387
>gb|ABD28670.2| RNA-directed DNA polymerase (Reverse transcriptase) [Medicago
truncatula]
Length = 642
Score = 65.1 bits (157), Expect(2) = 3e-20
Identities = 37/115 (32%), Positives = 64/115 (55%), Gaps = 1/115 (0%)
Frame = -1
Query: 637 SIFVLAQKLKRLKGALKSWHRNVFGNLGTQVRDEFAALESLKKDLERN-YNDVLANEIIN 461
++ VL L L LK W+++VFGN+ QV L ++ + + +ND L N
Sbjct: 26 NVRVLGSPLYVLSSKLKVWNKDVFGNILIQVTQAEKKLSDIQNHINTSGHNDNLMNAEKI 85
Query: 460 QENKVEGLLEQEESFWPQKSKVKWDNELDKSTKFFHALADMNRNKSYITEMENKG 296
+ ++ L+++E+FW +K+K+KW D++TK+FH L + +NK+ I KG
Sbjct: 86 AQTNLDLALQKQETFWVEKAKLKWHVGGDRNTKYFHRLTKI-KNKTKIISSLRKG 139
Score = 59.3 bits (142), Expect(2) = 3e-20
Identities = 30/63 (47%), Positives = 37/63 (58%)
Frame = -3
Query: 191 MPKSVT*EDNENLIKMPPLEEIKKSVFDLNPDSSPGSDGFTGHFFQTYWDIVGSDMVRAV 12
+PK V N L +P EE+K +VFDLN D +PG D F FFQ YW+IV D+ AV
Sbjct: 161 IPKLVDATTNRLLTMLPTKEEVKNAVFDLNSDDAPGPDVFGACFFQIYWNIVKKDVYEAV 220
Query: 11 QYF 3
F
Sbjct: 221 LDF 223
>emb|CAN74312.1| hypothetical protein VITISV_037520 [Vitis vinifera]
Length = 1915
Score = 72.8 bits (177), Expect(2) = 8e-18
Identities = 44/117 (37%), Positives = 65/117 (55%), Gaps = 1/117 (0%)
Frame = -1
Query: 655 EPMEGHSIFVLAQKLKRLKGALKSWHRNVFGNLGTQVRDEFAALESLKK-DLERNYNDVL 479
E EGH +KLK +K LK W+ VFG+L + + L + + + E N N L
Sbjct: 1004 EGWEGHKFM---RKLKFIKSKLKEWNTRVFGDLRERKKHILTDLGRIDRIEQEGNLNLEL 1060
Query: 478 ANEIINQENKVEGLLEQEESFWPQKSKVKWDNELDKSTKFFHALADMNRNKSYITEM 308
+E I + ++E LL +EE W QKS+VKW E D ++KFFH +A R++ YI +
Sbjct: 1061 VSERILRRKELEDLLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKYIKSL 1117
Score = 43.1 bits (100), Expect(2) = 8e-18
Identities = 30/97 (30%), Positives = 50/97 (51%), Gaps = 1/97 (1%)
Frame = -3
Query: 305 KQGATLADQKEIGEHIVQ*FTKKLKRVELQLDDDLINLMPKSVT*EDNENLIKMPPLEE- 129
++G TL + + I E IV F + E D I + + E++ + P EE
Sbjct: 1120 ERGETLNNIEVISEEIVNFFGNLYSKPEG--DSWKIEGIDWAPISEESAIWLDRPFSEEX 1177
Query: 128 IKKSVFDLNPDSSPGSDGFTGHFFQTYWDIVGSDMVR 18
++ +VF LN + +PG DGFT +Q WD++ D++R
Sbjct: 1178 VRMAVFQLNKEKAPGPDGFTLAVYQECWDVIKEDLMR 1214
>ref|XP_002450418.1| hypothetical protein SORBIDRAFT_05g005061 [Sorghum bicolor]
gi|241936261|gb|EES09406.1| hypothetical protein
SORBIDRAFT_05g005061 [Sorghum bicolor]
Length = 753
Score = 59.7 bits (143), Expect(2) = 9e-18
Identities = 37/111 (33%), Positives = 58/111 (52%)
Frame = -1
Query: 640 HSIFVLAQKLKRLKGALKSWHRNVFGNLGTQVRDEFAALESLKKDLERNYNDVLANEIIN 461
+S+ + KL L LKSW FG + ++R + LE L+ D RN +I+N
Sbjct: 274 NSVKDMKDKLFHLGEELKSWGGKTFGVVRKELRVQKKRLEQLRADPSRNTVSEEEQKIVN 333
Query: 460 QENKVEGLLEQEESFWPQKSKVKWDNELDKSTKFFHALADMNRNKSYITEM 308
++ L QEE W Q+S++ W +E D +TKFFH A R ++ I ++
Sbjct: 334 ---RIILLNYQEEIMWRQRSRITWLHEGDSNTKFFHQRASRRRIRNRIDKL 381
Score = 56.2 bits (134), Expect(2) = 9e-18
Identities = 27/66 (40%), Positives = 39/66 (59%), Gaps = 2/66 (3%)
Frame = -3
Query: 203 LINLMPKSVT*EDNENLIKMPPLEE--IKKSVFDLNPDSSPGSDGFTGHFFQTYWDIVGS 30
+++ +P+ VT D+ NL P E +K ++F + P SPG DGF HFFQ WD+ G
Sbjct: 418 VLDHIPRKVT--DDMNLFLCAPFNETEVKNALFQMFPTKSPGPDGFPAHFFQRNWDVCGD 475
Query: 29 DMVRAV 12
D+ R V
Sbjct: 476 DLTRMV 481
>pir||S65812 RNA-directed DNA polymerase (EC 2.7.7.49) (clone DW15) -
Arabidopsis thaliana retrotransposon Ta11-1
gi|976278|gb|AAA75254.1| reverse transcriptase
[Arabidopsis thaliana]
Length = 1333
Score = 61.2 bits (147), Expect(2) = 2e-17
Identities = 36/107 (33%), Positives = 55/107 (51%)
Frame = -1
Query: 628 VLAQKLKRLKGALKSWHRNVFGNLGTQVRDEFAALESLKKDLERNYNDVLANEIINQENK 449
++ KLK + AL W + N T++ AALE LE++ A+ + + +N
Sbjct: 272 LVLDKLKHCRSALSRWKKENNINSSTRITQARAALE-----LEQSSGFPRADLVFSLKND 326
Query: 448 VEGLLEQEESFWPQKSKVKWDNELDKSTKFFHALADMNRNKSYITEM 308
+ EE FW QKS+ KW + DK+T FFHA NR K +I ++
Sbjct: 327 LCKANHDEEVFWSQKSRAKWMHSGDKNTSFFHASVKDNRGKQHIDQL 373
Score = 53.5 bits (127), Expect(2) = 2e-17
Identities = 27/68 (39%), Positives = 36/68 (52%)
Frame = -3
Query: 206 DLINLMPKSVT*EDNENLIKMPPLEEIKKSVFDLNPDSSPGSDGFTGHFFQTYWDIVGSD 27
DL VT N LI EI+++VF + S+PG DGFTG FFQ YW I+
Sbjct: 409 DLFEDYQPRVTESMNNTLIAAVSKNEIREAVFAIRSSSAPGVDGFTGFFFQKYWSIICLQ 468
Query: 26 MVRAVQYF 3
+ + +Q F
Sbjct: 469 VTKEIQNF 476