BLASTX nr result
ID: Coptis23_contig00021957
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00021957
(1884 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis ... 751 0.0
emb|CBI17531.3| unnamed protein product [Vitis vinifera] 751 0.0
ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-de... 743 0.0
ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|2... 738 0.0
gb|AER08630.1| steroid 22-alpha hydroxylase [Populus euphratica] 737 0.0
>ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis vinifera]
Length = 487
Score = 751 bits (1940), Expect = 0.0
Identities = 362/487 (74%), Positives = 415/487 (85%), Gaps = 8/487 (1%)
Frame = +2
Query: 275 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 454
MS+L+F + +I L+I L KRK+ LNLPPGN GWPFLGETIGYLKPYSA + G+
Sbjct: 1 MSDLEFFLLLLASISVPLVILNLIKRKQSGLNLPPGNTGWPFLGETIGYLKPYSATSIGD 60
Query: 455 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 634
FMEQHISRFG+IYKS+LFG TIVS D+ LNRFILQN+G+LFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKWSML 120
Query: 635 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 814
VLVGDMH++MRTISLNF+S RLRTHL+ +V H LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 815 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 994
MAKHIMS++PGKPE+E+L++EY+TFMKGVVS PLNFPGTAYRKAL+SRS+ILK IE M
Sbjct: 181 LMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIELKM 240
Query: 995 DEKIRKTNGHS-DKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYF 1171
+E+I+K G + +EDDDLLGWVLKHSNL+TEQILDL+LS+LFAGHET+S++I+LAIYF
Sbjct: 241 EERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALAIYF 300
Query: 1172 LQGCPEAVQELREEHLRITKAKKQAGESGLNWEDYKQMEFTQCVINETLRLGNVVRFLHR 1351
L+GCP AVQ+LREEHL I++AKKQ+GE L+WEDYK+MEFTQCVI+ETLRLGNVVRFLHR
Sbjct: 301 LEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRFLHR 360
Query: 1352 KALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQKG-------KTSAPT 1510
KALKDVRYKGYDIPCGWKVLPV AAVHLDP FDQPQ FNPWRWQ S T
Sbjct: 361 KALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQNNGNRGNSTSWSTAT 420
Query: 1511 SNNFMPFGGGPRLCAGSELAKLEMAVFVHHLVLNYNWELAEQDQPLAFPFVEFPKGLPIK 1690
+ NFMPFGGGPRLCAGSELAKLEMAVF+HHLVLNYNWEL ++DQ AFPFV+FPKGLPIK
Sbjct: 421 NQNFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNYNWELVDKDQAFAFPFVDFPKGLPIK 480
Query: 1691 VHDQQLT 1711
V T
Sbjct: 481 VRHHNFT 487
>emb|CBI17531.3| unnamed protein product [Vitis vinifera]
Length = 488
Score = 751 bits (1940), Expect = 0.0
Identities = 362/488 (74%), Positives = 416/488 (85%), Gaps = 9/488 (1%)
Frame = +2
Query: 275 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 454
MS+L+F + +I L+I L KRK+ LNLPPGN GWPFLGETIGYLKPYSA + G+
Sbjct: 1 MSDLEFFLLLLASISVPLVILNLIKRKQSGLNLPPGNTGWPFLGETIGYLKPYSATSIGD 60
Query: 455 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 634
FMEQHISRFG+IYKS+LFG TIVS D+ LNRFILQN+G+LFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKWSML 120
Query: 635 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 814
VLVGDMH++MRTISLNF+S RLRTHL+ +V H LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 815 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 994
MAKHIMS++PGKPE+E+L++EY+TFMKGVVS PLNFPGTAYRKAL+SRS+ILK IE M
Sbjct: 181 LMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIELKM 240
Query: 995 DEKIRKTNGHS-DKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYF 1171
+E+I+K G + +EDDDLLGWVLKHSNL+TEQILDL+LS+LFAGHET+S++I+LAIYF
Sbjct: 241 EERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALAIYF 300
Query: 1172 LQGCPEAVQELREEHLRITKAKKQAGESGLNWEDYKQMEFTQCVINETLRLGNVVRFLHR 1351
L+GCP AVQ+LREEHL I++AKKQ+GE L+WEDYK+MEFTQCVI+ETLRLGNVVRFLHR
Sbjct: 301 LEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRFLHR 360
Query: 1352 KALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQKG--------KTSAP 1507
KALKDVRYKGYDIPCGWKVLPV AAVHLDP FDQPQ FNPWRWQ+ S
Sbjct: 361 KALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQQNNGNRGNSTSWSTA 420
Query: 1508 TSNNFMPFGGGPRLCAGSELAKLEMAVFVHHLVLNYNWELAEQDQPLAFPFVEFPKGLPI 1687
T+ NFMPFGGGPRLCAGSELAKLEMAVF+HHLVLNYNWEL ++DQ AFPFV+FPKGLPI
Sbjct: 421 TNQNFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNYNWELVDKDQAFAFPFVDFPKGLPI 480
Query: 1688 KVHDQQLT 1711
KV T
Sbjct: 481 KVRHHNFT 488
>ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-deoxocathasterone or
6-oxocampestanol to cathasterone [Populus trichocarpa]
gi|222853127|gb|EEE90674.1| cytochrome P450 probable
campestanol to 6-deoxocathasterone or 6-oxocampestanol to
cathasterone [Populus trichocarpa]
Length = 486
Score = 743 bits (1919), Expect = 0.0
Identities = 354/482 (73%), Positives = 416/482 (86%), Gaps = 9/482 (1%)
Frame = +2
Query: 275 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 454
MS + + + P+IL+LLL++ L +RK+ NLPPGNMGWPFLGETIGYLKPYSA + GE
Sbjct: 1 MSHSELVVFLLPSILSLLLLFILVQRKQVRFNLPPGNMGWPFLGETIGYLKPYSATSIGE 60
Query: 455 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 634
FMEQHISR+GKIYKS+LFG TIVS DA LNRFILQN+GRLFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPKSIGGILGKWSML 120
Query: 635 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 814
VLVGDMH++MR ISLNF+S ARLRTHL+++VE LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRDMRIISLNFLSHARLRTHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 815 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 994
MAKHIMS++PGKPE+E+L++EY+TFMKGVVS P+N PGT YRKALKSRS ILK IE+ M
Sbjct: 181 LMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPINLPGTPYRKALKSRSIILKFIERKM 240
Query: 995 DEKIRKTNGHSDKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYFL 1174
+E+I +T G + +EDDDLLGWVLKHSNL+TEQILDLILS+LFAGHET+S+SI+LAIYFL
Sbjct: 241 EERIAETKGGVENLEDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAIYFL 300
Query: 1175 QGCPEAVQELREEHLRITKAKKQAGESGLNWEDYKQMEFTQCVINETLRLGNVVRFLHRK 1354
Q CP A+Q+L+EEH++I++AKK++GE+ LNW+DYK+MEFTQCVI+ETLRLGNVVRFLHRK
Sbjct: 301 QACPGAIQQLKEEHIQISRAKKRSGETELNWDDYKKMEFTQCVISETLRLGNVVRFLHRK 360
Query: 1355 ALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQK---------GKTSAP 1507
ALKDVRYKGYDIPCGWKVLPV +AVHLD FDQPQ FNPWRWQ+ +
Sbjct: 361 ALKDVRYKGYDIPCGWKVLPVISAVHLDSTLFDQPQHFNPWRWQQHNNARGASTSSSGTT 420
Query: 1508 TSNNFMPFGGGPRLCAGSELAKLEMAVFVHHLVLNYNWELAEQDQPLAFPFVEFPKGLPI 1687
+SN FMPFGGGPRLCAGSELAKLEMAVF+HHLVLN++WEL + DQ AFPFV+FPKGLPI
Sbjct: 421 SSNYFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNFHWELVDTDQAFAFPFVDFPKGLPI 480
Query: 1688 KV 1693
+V
Sbjct: 481 RV 482
>ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|222836894|gb|EEE75287.1|
predicted protein [Populus trichocarpa]
Length = 488
Score = 738 bits (1905), Expect = 0.0
Identities = 351/482 (72%), Positives = 414/482 (85%), Gaps = 9/482 (1%)
Frame = +2
Query: 275 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 454
MS + + + P+IL+LLL++ L +RK+ NLPPGNMGWPFLGETIGYLKPYSA + GE
Sbjct: 1 MSHSELVVLLLPSILSLLLVFILMQRKQARFNLPPGNMGWPFLGETIGYLKPYSATSIGE 60
Query: 455 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 634
FMEQHISR+GKIYKS+LFG TIVS DA LNRFILQ++GRLFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQSEGRLFECSYPRSIGGILGKWSML 120
Query: 635 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 814
VLVGDMH+NMR+ISLNF+S ARLR+HL+++VE LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRNMRSISLNFLSHARLRSHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 815 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 994
MAKH+MS++PGKPE+E+L++EY+TFMKGVVS PLNFPGT YRKALKSRS ILK IEQ M
Sbjct: 181 LMAKHMMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTPYRKALKSRSIILKFIEQKM 240
Query: 995 DEKIRKTNGHSDKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYFL 1174
+E+I + + +E+DDLLGWVLKHSNL+TEQILDLILS+LFAGHET+S+SI+LAIYFL
Sbjct: 241 EERIARLKEGVEDLEEDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAIYFL 300
Query: 1175 QGCPEAVQELREEHLRITKAKKQAGESGLNWEDYKQMEFTQCVINETLRLGNVVRFLHRK 1354
Q CP A+Q+L+EEH+ I++AKKQ+GE +NW+DYK+MEFTQCVI+ETLRLGNVVRFLHRK
Sbjct: 301 QACPGAIQQLKEEHIEISRAKKQSGEMEINWDDYKKMEFTQCVISETLRLGNVVRFLHRK 360
Query: 1355 ALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQK---------GKTSAP 1507
A++DVRYKGYDIPCGWKVLPV +AVHLD FDQPQ FNPWRWQ +A
Sbjct: 361 AVRDVRYKGYDIPCGWKVLPVISAVHLDSTVFDQPQQFNPWRWQHNNARGSSTCSSAAAA 420
Query: 1508 TSNNFMPFGGGPRLCAGSELAKLEMAVFVHHLVLNYNWELAEQDQPLAFPFVEFPKGLPI 1687
+SN+FMPFGGGPRLCAGSELAKLEMAVF+HHLVLN++WEL DQ AFPFV+FPKGLPI
Sbjct: 421 SSNHFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNFHWELVGADQAFAFPFVDFPKGLPI 480
Query: 1688 KV 1693
+V
Sbjct: 481 RV 482
>gb|AER08630.1| steroid 22-alpha hydroxylase [Populus euphratica]
Length = 490
Score = 737 bits (1902), Expect = 0.0
Identities = 352/484 (72%), Positives = 412/484 (85%), Gaps = 11/484 (2%)
Frame = +2
Query: 275 MSELDFIFSVAPAILALLLIWRLFKRKEKPLNLPPGNMGWPFLGETIGYLKPYSAITTGE 454
MS + + P+IL+LLL++ L +RK+ NLPPGNMGWPFLGETIGYLKPYSA + GE
Sbjct: 1 MSHSELAVLLLPSILSLLLVFILMQRKQARFNLPPGNMGWPFLGETIGYLKPYSATSIGE 60
Query: 455 FMEQHISRFGKIYKSHLFGNQTIVSTDAELNRFILQNDGRLFECSYPSSIGGILGKWSML 634
FMEQHISR+GKIYKS+LFG TIVS DA LNRFILQ++GRLFECSYP SIGGILGKWSML
Sbjct: 61 FMEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQSEGRLFECSYPRSIGGILGKWSML 120
Query: 635 VLVGDMHKNMRTISLNFMSSARLRTHLIQDVETHALLILKSWKANTAFSAQDEAKKFTFN 814
VLVGDMH+NMR ISLNF+S ARLR+HL+++VE LL+L SWK N FSAQDEAKKFTFN
Sbjct: 121 VLVGDMHRNMRNISLNFLSHARLRSHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFN 180
Query: 815 FMAKHIMSMEPGKPESEKLRREYITFMKGVVSPPLNFPGTAYRKALKSRSSILKVIEQNM 994
MAKH+MS++PGKPE+E+L++EY+TFMKGVVS PLNFPGT YRKALKSRS+ILK IEQ M
Sbjct: 181 LMAKHMMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTPYRKALKSRSTILKFIEQKM 240
Query: 995 DEKIRKTNGHSDKVEDDDLLGWVLKHSNLTTEQILDLILSMLFAGHETTSISISLAIYFL 1174
DE+I + + +E+DDLLGWVLKHSNL+TEQILDLILS+LFAGHET+S+SI+LAIYFL
Sbjct: 241 DERIARLKEGVEDLEEDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAIYFL 300
Query: 1175 QGCPEAVQELREEHLRITKAKKQAGESGLNWEDYKQMEFTQCVINETLRLGNVVRFLHRK 1354
Q CP A+Q+L+EEH+ I++AKKQ+GE +NW+DYK+MEFTQCVI+ETLRLGNVVRFLHRK
Sbjct: 301 QACPGAIQQLKEEHIEISRAKKQSGEMEMNWDDYKKMEFTQCVISETLRLGNVVRFLHRK 360
Query: 1355 ALKDVRYKGYDIPCGWKVLPVFAAVHLDPLNFDQPQAFNPWRWQ-----------KGKTS 1501
A++DVRYKGYDIPCGWKVLPV +AVHLD FDQPQ FNPWRWQ +
Sbjct: 361 AVRDVRYKGYDIPCGWKVLPVISAVHLDSTVFDQPQQFNPWRWQHNNARGSSTCSSAAAA 420
Query: 1502 APTSNNFMPFGGGPRLCAGSELAKLEMAVFVHHLVLNYNWELAEQDQPLAFPFVEFPKGL 1681
A +SN FMPFGGGPRLCAGSELAKLEMAVF+HHLVLN++WEL DQ AFPFV+FPKGL
Sbjct: 421 AASSNYFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNFHWELVGTDQAFAFPFVDFPKGL 480
Query: 1682 PIKV 1693
PI+V
Sbjct: 481 PIRV 484