BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00021150
         (684 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2;...   236   4e-60
sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Fl...   233   3e-59
pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthas...   203   3e-50
gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]             199   3e-49
gb|ACO90254.1| pathogenesis-related (PR)-10-related norcoclaurin...   137   3e-30

>sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2; AltName:
           Full=Pathogenesis related protein 10A; Short=CjPR10A;
           Flags: Precursor gi|289176978|dbj|BAF45338.2|
           pathogenesis related protein 10A [Coptis japonica var.
           dissecta]
          Length = 196

 Score =  236 bits (601), Expect = 4e-60
 Identities = 125/194 (64%), Positives = 148/194 (76%), Gaps = 1/194 (0%)
 Frame = -3

Query: 595 VLFVLLMFLGTMNCNNLVVSGRPLLHGITNKESTVSKVLEHELKVAASADEVWSVYGSPE 416
           VL V LMF+GT+NC  L+ +GRPLLH +T KE TV  +L HEL+VAASADEVWSV GSPE
Sbjct: 6   VLVVFLMFIGTINCERLIFNGRPLLHRVT-KEETV--MLYHELEVAASADEVWSVEGSPE 62

Query: 415 LPMHLMEMLP-GAFEXXXXXXXXXXXXXXDLTFPPGEFPHAYKEKFVFIDHKLRILKVQI 239
           L +HL ++LP G F               D+TFPPG+FPH Y+EKFVF DHK R   V+ 
Sbjct: 63  LGLHLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEQ 122

Query: 238 INGDYANLGVTYYMDTIIVVETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMAEA 59
           I+GD+ +LGVTYYMDTI VV TGPDSCVIKS+ EY+VKPEFAKIV+PLIDTV +A M+EA
Sbjct: 123 IDGDFFDLGVTYYMDTIRVVATGPDSCVIKSTTEYHVKPEFAKIVKPLIDTVPLAIMSEA 182

Query: 58  VAKKVLEEKHKSSE 17
           +AK VLE KHKSSE
Sbjct: 183 IAKVVLENKHKSSE 196


>sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Flags: Precursor
           gi|38492394|gb|AAR22502.1| (S)-norcoclaurine synthase
           [Thalictrum flavum subsp. glaucum]
           gi|226897754|gb|ACO90248.1| norcoclaurine synthase
           [Thalictrum flavum]
          Length = 210

 Score =  233 bits (593), Expect = 3e-59
 Identities = 117/197 (59%), Positives = 147/197 (74%), Gaps = 2/197 (1%)
 Frame = -3

Query: 598 EVLFVLLMFLGTMNCNNLVVSGRPLLH--GITNKESTVSKVLEHELKVAASADEVWSVYG 425
           EV+FV LM LGT+NC  L+++GRP LH  GI N+ STV+KV+ HEL+VAASAD++W+VY 
Sbjct: 5   EVVFVFLMLLGTINCQKLILTGRPFLHHQGIINQVSTVTKVIHHELEVAASADDIWTVYS 64

Query: 424 SPELPMHLMEMLPGAFEXXXXXXXXXXXXXXDLTFPPGEFPHAYKEKFVFIDHKLRILKV 245
            P L  HL ++LPGAFE              D+TF PGEFPH YKEKF+ +D++ R+ KV
Sbjct: 65  WPGLAKHLPDLLPGAFEKLEIIGDGGVGTILDMTFVPGEFPHEYKEKFILVDNEHRLKKV 124

Query: 244 QIINGDYANLGVTYYMDTIIVVETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMA 65
           Q+I G Y +LGVTYYMDTI VV TG DSCVIKSS EY+VKPEF KIVEPLI T  +A+MA
Sbjct: 125 QMIEGGYLDLGVTYYMDTIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMA 184

Query: 64  EAVAKKVLEEKHKSSEE 14
           +A++K VLE K KS+ +
Sbjct: 185 DAISKLVLEHKSKSNSD 201


>pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927323|pdb|2VNE|B Chain B, The
           X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927324|pdb|2VQ5|A Chain A, X-Ray
           Structure Of Norcoclaurine Synthase From Thalictrum
           Flavum In Complex With Dopamine And Hydroxybenzaldehyde
           gi|195927325|pdb|2VQ5|B Chain B, X-Ray Structure Of
           Norcoclaurine Synthase From Thalictrum Flavum In Complex
           With Dopamine And Hydroxybenzaldehyde
          Length = 201

 Score =  203 bits (516), Expect = 3e-50
 Identities = 104/180 (57%), Positives = 130/180 (72%), Gaps = 2/180 (1%)
 Frame = -3

Query: 547 LVVSGRPLLH--GITNKESTVSKVLEHELKVAASADEVWSVYGSPELPMHLMEMLPGAFE 374
           L+++GRP LH  GI N+ STV+KV+ HEL+VAASAD++W+VY  P L  HL ++LPGAFE
Sbjct: 7   LILTGRPFLHHQGIINQVSTVTKVIHHELEVAASADDIWTVYSWPGLAKHLPDLLPGAFE 66

Query: 373 XXXXXXXXXXXXXXDLTFPPGEFPHAYKEKFVFIDHKLRILKVQIINGDYANLGVTYYMD 194
                         D TF PGEFPH YKEKF+ +D++ R+ KVQ I G Y +LGVTYY D
Sbjct: 67  KLEIIGDGGVGTILDXTFVPGEFPHEYKEKFILVDNEHRLKKVQXIEGGYLDLGVTYYXD 126

Query: 193 TIIVVETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMAEAVAKKVLEEKHKSSEE 14
           TI VV TG DSCVIKSS EY+VKPEF KIVEPLI T  +A+ A+A++K VLE K KS+ +
Sbjct: 127 TIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAXADAISKLVLEHKSKSNSD 186


>gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]
          Length = 190

 Score =  199 bits (507), Expect = 3e-49
 Identities = 102/175 (58%), Positives = 129/175 (73%), Gaps = 2/175 (1%)
 Frame = -3

Query: 532 RPLLH--GITNKESTVSKVLEHELKVAASADEVWSVYGSPELPMHLMEMLPGAFEXXXXX 359
           RP L+  GI N+ STV+K + HEL+VAASAD++WSVY  P L  HL ++LPGAFE     
Sbjct: 1   RPFLNRQGIINQVSTVTKGVHHELEVAASADDIWSVYSWPGLAKHLPDLLPGAFEKLEII 60

Query: 358 XXXXXXXXXDLTFPPGEFPHAYKEKFVFIDHKLRILKVQIINGDYANLGVTYYMDTIIVV 179
                    D+TF PGEFPH YKEKF+ +D++ R+ KVQ+I G Y +LGVTYYMDTI V+
Sbjct: 61  GDGGVGTILDMTFTPGEFPHEYKEKFILVDNEHRLKKVQMIEGGYLDLGVTYYMDTIQVI 120

Query: 178 ETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMAEAVAKKVLEEKHKSSEE 14
            TG +SCVIKSS EY+VKPEF KIVEPLI T  +A+MAEA++K VLE K+KS+ +
Sbjct: 121 PTGTNSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMAEAISKLVLEHKYKSNSD 175


>gb|ACO90254.1| pathogenesis-related (PR)-10-related norcoclaurine synthase-like
           protein [Eschscholzia californica]
          Length = 184

 Score =  137 bits (344), Expect = 3e-30
 Identities = 69/160 (43%), Positives = 101/160 (63%), Gaps = 3/160 (1%)
 Frame = -3

Query: 487 KVLEHELKVAASADEVWSVYGSPELPMHLMEMLPGAFEXXXXXXXXXXXXXXDL--TFPP 314
           K LE++ KV ASAD++W+VY SP+LP  ++++LP  FE               L   +PP
Sbjct: 3   KELENQRKVEASADDIWAVYSSPDLPKLIVKLLPSVFESIDIVEGTGGSVGTVLHLVYPP 62

Query: 313 GEFPHAYKEKFVFIDHKLRILKVQIINGDYANLGVTYYMDTIIVVETGPDSCVIKSSMEY 134
           G  PH YKEKF+ IDH+ R+ +V+ I G Y ++GVT+YMD+  ++E GP+SC+IKS   Y
Sbjct: 63  GSIPHTYKEKFITIDHEKRLKEVRQIQGGYLDMGVTFYMDSFHILENGPNSCIIKSMTTY 122

Query: 133 YV-KPEFAKIVEPLIDTVSVASMAEAVAKKVLEEKHKSSE 17
            V   E  + V P I   S+  MAEA++K VL++K  S++
Sbjct: 123 EVPNEEVGEKVSPFISIDSLVGMAEAISKYVLDKKKSSAK 162