BLASTX nr result
ID: Coptis23_contig00018352
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00018352
(1797 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti... 716 0.0
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2... 709 0.0
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2... 684 0.0
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ... 684 0.0
gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum] 670 0.0
>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
Length = 882
Score = 716 bits (1848), Expect = 0.0
Identities = 343/529 (64%), Positives = 418/529 (79%), Gaps = 3/529 (0%)
Frame = -3
Query: 1795 EIRPCLPMEKLVVYRLNVEHFTKDKSSKLPTGVAGTFSGLIEKSQHFKGLGVNAILLEPI 1616
++ P +PMEKLVVYRLNV HFTKD+SS++ + +AGTFSGL+EK HFK LGVNA+LLEPI
Sbjct: 360 DVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPI 419
Query: 1615 FPFNEQKGPYYPYHFFSPMHLYGGPTHDGLSAINSMKEMVKVMHANNIEVLLEVVFTHTA 1436
F F+EQKGPY+P+HFFSPM++YG P+ +S INS+KEMVK +HAN IEVLLEVVFTHTA
Sbjct: 420 FSFDEQKGPYFPFHFFSPMNVYG-PSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTA 478
Query: 1435 ECGDYASKTTGLQGIDKKSYYGFNEDMETVDRSALNCNHPIVQWMVLDSLRYWVTEFHVD 1256
E G LQGID YY N D + R+ALNCN+ IVQ M++DSLRYWVTEFHVD
Sbjct: 479 ESG-------ALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVD 531
Query: 1255 GFCFLNASYLLRGLNGEYLSRPPLIEAITFDPLLSKTKLIADSWDPYGLDSKEIRFPHWK 1076
GFCF+NAS LLRG +GEYLSRPPL+E I FDPLLSKTK+IAD WDP + KEIRFPHWK
Sbjct: 532 GFCFINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWK 591
Query: 1075 RWAEINSKFCYDVRNFLKGEGLLSNLATRICGSADIFSDGRGPTFSFNYVARNFGLPXXX 896
RWAE+N++FC DVRNFL+GEGL S+ ATR+CGS DIF DGRGP FSFN+ +NFGLP
Sbjct: 592 RWAEVNTRFCNDVRNFLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVD 650
Query: 895 XXXXXXXXXXXXXSWNCGEEGPTSKSIVLEKRLKQIRNFLFILYISMGVPVLNMGDECGQ 716
SWNCG+EGPT+K+ VLE+RLKQIRNFLFILY+S+GVP+LNMGDECGQ
Sbjct: 651 LVSFSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQ 710
Query: 715 SSSGSPLYGHRKPFDWDALRTTFGVQITLFIAFLTSLRTRRGDFLQKRNFMKVENVDWQG 536
SS GSP YG RKPF+W++++T FG+Q FI+FL+SLR+RR D LQ+R+F+K E++DW G
Sbjct: 711 SSGGSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHG 770
Query: 535 SDQAQPRWEDPSSKFLTMILKADNDDTESKSSGGS---DLFVGFNGSDSSETVILPPPSQ 365
SDQ+ PRW+DPSSKFL M LKA+N + + S S DLF+ FN +D S VILPPP
Sbjct: 771 SDQSPPRWDDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPT 830
Query: 364 GLAWFRLVDTALPFPEFFSTDGEPVIEQTTGLLAYVMKSHSCALFEAQS 218
G+ W RLVDTALPFP FF+ DGE ++++ +GL+ Y M+SHSCALFEA +
Sbjct: 831 GMVWHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANT 879
>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
isoamylase, putative [Ricinus communis]
Length = 872
Score = 709 bits (1829), Expect = 0.0
Identities = 348/527 (66%), Positives = 411/527 (77%), Gaps = 3/527 (0%)
Frame = -3
Query: 1795 EIRPCLPMEKLVVYRLNVEHFTKDKSSKLPTGVAGTFSGLIEKSQHFKGLGVNAILLEPI 1616
+IRP L MEKL+VYRLNV+ FT+ KS KL + +AGTF+GLIEK HF+ LGVNA+LLEPI
Sbjct: 351 DIRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPI 410
Query: 1615 FPFNEQKGPYYPYHFFSPMHLYGGPTHDGLSAINSMKEMVKVMHANNIEVLLEVVFTHTA 1436
FPF+EQKGPY+PYHFFSP ++YG P+ +SAI SMKEMVK +HAN IEVLLEVVFTHTA
Sbjct: 411 FPFDEQKGPYFPYHFFSPSNIYG-PSGGSISAITSMKEMVKELHANRIEVLLEVVFTHTA 469
Query: 1435 ECGDYASKTTGLQGIDKKSYYGFNEDMETVDRSALNCNHPIVQWMVLDSLRYWVTEFHVD 1256
E G LQGID SYY M++ R+ALNCN+PIVQ M+LDSL++WVTEFH+D
Sbjct: 470 EGG-------ALQGIDDFSYYYTKSSMDS--RNALNCNYPIVQRMILDSLQHWVTEFHID 520
Query: 1255 GFCFLNASYLLRGLNGEYLSRPPLIEAITFDPLLSKTKLIADSWDPYGLDSKEIRFPHWK 1076
GFCF+NAS LL G +GE+LSRPPL+EAI FDP+LSKTK+IAD W P KE FPHWK
Sbjct: 521 GFCFINASALLTGFHGEHLSRPPLVEAIAFDPILSKTKIIADPWHPEHRIPKETCFPHWK 580
Query: 1075 RWAEINSKFCYDVRNFLKGEGLLSNLATRICGSADIFSDGRGPTFSFNYVARNFGLPXXX 896
RWAEIN KFC DVRNFL+GE LL +LATR+CGS DIFS+GRGP FSFNY+ARN GLP
Sbjct: 581 RWAEINPKFCIDVRNFLRGESLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVD 640
Query: 895 XXXXXXXXXXXXXSWNCGEEGPTSKSIVLEKRLKQIRNFLFILYISMGVPVLNMGDECGQ 716
SWNCGEEGPT+K+ VLE+RLKQIRN+LFILY+S+GVPVLNMGDECGQ
Sbjct: 641 LVSFSGGELGSELSWNCGEEGPTNKTAVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQ 700
Query: 715 SSSGSPLYGHRKPFDWDALRTTFGVQITLFIAFLTSLRTRRGDFLQKRNFMKVENVDWQG 536
SS GS YG RKPFDW+AL T+FG Q+T FI+FL+SLR RR D LQKRNF+K EN+DW G
Sbjct: 701 SSRGSISYGDRKPFDWNALSTSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHG 760
Query: 535 SDQAQPRWEDPSSKFLTMILKADNDDTESKSSGGS---DLFVGFNGSDSSETVILPPPSQ 365
+DQ+ PRWEDP+ KFL M LK D +++ S + DLF+ FN + +E+VILPP +
Sbjct: 761 NDQSPPRWEDPTCKFLAMTLKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPE 820
Query: 364 GLAWFRLVDTALPFPEFFSTDGEPVIEQTTGLLAYVMKSHSCALFEA 224
G+ W RLVDTALPFP FFS DGEPV+EQ GL+AY M SHSC LFEA
Sbjct: 821 GMIWRRLVDTALPFPGFFSEDGEPVVEQIAGLIAYKMNSHSCTLFEA 867
>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
[Cucumis sativus]
Length = 885
Score = 684 bits (1766), Expect = 0.0
Identities = 333/532 (62%), Positives = 409/532 (76%), Gaps = 3/532 (0%)
Frame = -3
Query: 1795 EIRPCLPMEKLVVYRLNVEHFTKDKSSKLPTGVAGTFSGLIEKSQHFKGLGVNAILLEPI 1616
E+ P LPMEKL VYRLNVE FT DKSS+LP +AGTFSGL +K HFK LGVNA+LLEPI
Sbjct: 364 EVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPI 423
Query: 1615 FPFNEQKGPYYPYHFFSPMHLYGGPTHDGLSAINSMKEMVKVMHANNIEVLLEVVFTHTA 1436
F F+E++GPY+P+HFFSP + YG P+ +SAINSMKEMVK +HAN +EV+LEVV+THT+
Sbjct: 424 FQFDEKEGPYFPFHFFSPTNNYG-PSGASISAINSMKEMVKELHANGVEVILEVVYTHTS 482
Query: 1435 ECGDYASKTTGLQGIDKKSYYGFNEDMETVDRSALNCNHPIVQWMVLDSLRYWVTEFHVD 1256
G LQGID SYY N ++SALNCN+PIVQ ++LDSLRYWVTEFHVD
Sbjct: 483 GNG-------ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVD 535
Query: 1255 GFCFLNASYLLRGLNGEYLSRPPLIEAITFDPLLSKTKLIADSWDPYGLDSKEIRFPHWK 1076
GFCF+NAS+LLRG +GE LSRPP +EAI FDPLLSKTKL+AD WDP L+SKE RFPHWK
Sbjct: 536 GFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWK 595
Query: 1075 RWAEINSKFCYDVRNFLKGEGLLSNLATRICGSADIFSDGRGPTFSFNYVARNFGLPXXX 896
RWAE+NSKFC D+R+F +GEGL+S+LATR+CGS D+FSDGRGP FSFN++ARN GLP
Sbjct: 596 RWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 655
Query: 895 XXXXXXXXXXXXXSWNCGEEGPTSKSIVLEKRLKQIRNFLFILYISMGVPVLNMGDECGQ 716
SWNCGEEGPTS VLEKRLKQIRNF+F+L++S+GVPVLNMGDECGQ
Sbjct: 656 LVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQ 715
Query: 715 SSSGSPLYGHRKPFDWDALRTTFGVQITLFIAFLTSLRTRRGDFLQKRNFMKVENVDWQG 536
SS GS + ++ F+WD L+T FG Q T FIAFL+S R+RR D Q RNF+K EN+DW
Sbjct: 716 SSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFD 775
Query: 535 SDQAQPRWEDPSSKFLTMILKADNDDTESKSSG---GSDLFVGFNGSDSSETVILPPPSQ 365
++Q+ P+WED S KFL ++L+AD ++ ES + S++F+ FN SD SE+V LP P +
Sbjct: 776 NNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLE 835
Query: 364 GLAWFRLVDTALPFPEFFSTDGEPVIEQTTGLLAYVMKSHSCALFEAQSPVD 209
G +WFR+VDTALPFP FFS+DGE V TG + Y +++HSCALFEA+S D
Sbjct: 836 GTSWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKSAND 885
>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
Length = 885
Score = 684 bits (1766), Expect = 0.0
Identities = 333/532 (62%), Positives = 409/532 (76%), Gaps = 3/532 (0%)
Frame = -3
Query: 1795 EIRPCLPMEKLVVYRLNVEHFTKDKSSKLPTGVAGTFSGLIEKSQHFKGLGVNAILLEPI 1616
E+ P LPMEKL VYRLNVE FT DKSS+LP +AGTFSGL +K HFK LGVNA+LLEPI
Sbjct: 364 EVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPI 423
Query: 1615 FPFNEQKGPYYPYHFFSPMHLYGGPTHDGLSAINSMKEMVKVMHANNIEVLLEVVFTHTA 1436
F F+E++GPY+P+HFFSP + YG P+ +SAINSMKEMVK +HAN +EV+LEVV+THT+
Sbjct: 424 FQFDEKEGPYFPFHFFSPTNNYG-PSGASISAINSMKEMVKELHANGVEVILEVVYTHTS 482
Query: 1435 ECGDYASKTTGLQGIDKKSYYGFNEDMETVDRSALNCNHPIVQWMVLDSLRYWVTEFHVD 1256
G LQGID SYY N ++SALNCN+PIVQ ++LDSLRYWVTEFHVD
Sbjct: 483 GNG-------ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVD 535
Query: 1255 GFCFLNASYLLRGLNGEYLSRPPLIEAITFDPLLSKTKLIADSWDPYGLDSKEIRFPHWK 1076
GFCF+NAS+LLRG +GE LSRPP +EAI FDPLLSKTKL+AD WDP L+SKE RFPHWK
Sbjct: 536 GFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWK 595
Query: 1075 RWAEINSKFCYDVRNFLKGEGLLSNLATRICGSADIFSDGRGPTFSFNYVARNFGLPXXX 896
RWAE+NSKFC D+R+F +GEGL+S+LATR+CGS D+FSDGRGP FSFN++ARN GLP
Sbjct: 596 RWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 655
Query: 895 XXXXXXXXXXXXXSWNCGEEGPTSKSIVLEKRLKQIRNFLFILYISMGVPVLNMGDECGQ 716
SWNCGEEGPTS VLEKRLKQIRNF+F+L++S+GVPVLNMGDECGQ
Sbjct: 656 LVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQ 715
Query: 715 SSSGSPLYGHRKPFDWDALRTTFGVQITLFIAFLTSLRTRRGDFLQKRNFMKVENVDWQG 536
SS GS + ++ F+WD L+T FG Q T FIAFL+S R+RR D Q RNF+K EN+DW
Sbjct: 716 SSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFD 775
Query: 535 SDQAQPRWEDPSSKFLTMILKADNDDTESKSSG---GSDLFVGFNGSDSSETVILPPPSQ 365
++Q+ P+WED S KFL ++L+AD ++ ES + S++F+ FN SD SE+V LP P +
Sbjct: 776 NNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLE 835
Query: 364 GLAWFRLVDTALPFPEFFSTDGEPVIEQTTGLLAYVMKSHSCALFEAQSPVD 209
G +WFR+VDTALPFP FFS+DGE V TG + Y +++HSCALFEA+S D
Sbjct: 836 GTSWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKSAND 885
>gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]
Length = 857
Score = 670 bits (1729), Expect = 0.0
Identities = 329/523 (62%), Positives = 403/523 (77%), Gaps = 4/523 (0%)
Frame = -3
Query: 1780 LPMEKLVVYRLNVEHFTKDKSSKLPTGVAGTFSGLIEKSQHFKGLGVNAILLEPIFPFNE 1601
L MEKLVVYRLNV+HFT+ +SS+L +AGTFSGL +K QHFK LGVNA+LLEP+F F+E
Sbjct: 338 LDMEKLVVYRLNVKHFTEHESSQLSGDLAGTFSGLAKKLQHFKDLGVNAVLLEPVFTFDE 397
Query: 1600 QKGPYYPYHFFSPMHLYGGPTHDGLSAINSMKEMVKVMHANNIEVLLEVVFTHTAECGDY 1421
+KGPY+P HFFSP++LYG P+ D S INSMKEMVK MHAN IEV++EVVF++TAE G
Sbjct: 398 EKGPYFPCHFFSPVNLYG-PSGDPESTINSMKEMVKTMHANGIEVIMEVVFSNTAEVG-- 454
Query: 1420 ASKTTGLQGIDKKSYYGFNEDMETVD-RSALNCNHPIVQWMVLDSLRYWVTEFHVDGFCF 1244
LQGID SYY N + + SALNCN+PIVQ ++LDSLR+WVTEFH+DGF F
Sbjct: 455 -----ALQGIDDLSYYYANGVVGGLKVHSALNCNYPIVQNLILDSLRHWVTEFHIDGFSF 509
Query: 1243 LNASYLLRGLNGEYLSRPPLIEAITFDPLLSKTKLIADSWDPYGLDSKEIRFPHWKRWAE 1064
+NAS+LLRG +GEYLSRPPL+EAI FDP+L KTK+IAD WDP +++KEIRFPHW RWAE
Sbjct: 510 VNASHLLRGYHGEYLSRPPLVEAIAFDPVLWKTKIIADCWDPNDMETKEIRFPHWMRWAE 569
Query: 1063 INSKFCYDVRNFLKGEGLLSNLATRICGSADIFSDGRGPTFSFNYVARNFGLPXXXXXXX 884
IN+ F DVRNFL+GE LLSNLATR+CGS D++SDGRGP FSFNY++ NFGL
Sbjct: 570 INTNFRSDVRNFLRGESLLSNLATRLCGSGDMYSDGRGPAFSFNYISGNFGLSLVDLVSF 629
Query: 883 XXXXXXXXXSWNCGEEGPTSKSIVLEKRLKQIRNFLFILYISMGVPVLNMGDECGQSSSG 704
SWNCGEEGPT+ + VLE+RLKQIRNFLFILY+S+GVP+LNMGDECG SS G
Sbjct: 630 SNAGLEAELSWNCGEEGPTNSTAVLERRLKQIRNFLFILYVSLGVPILNMGDECGHSSGG 689
Query: 703 SPLYGHRKPFDWDALRTTFGVQITLFIAFLTSLRTRRGDFLQKRNFMKVENVDWQGSDQA 524
S G+ KPF+W AL+T FG Q FI+FLTSLRTRR D LQ R+F+K EN++W+G D+A
Sbjct: 690 SLANGNTKPFNWAALKTGFGKQTIQFISFLTSLRTRRSDLLQSRSFLKEENIEWRGIDEA 749
Query: 523 QPRWEDPSSKFLTMILKADNDDTESKSSGG---SDLFVGFNGSDSSETVILPPPSQGLAW 353
P+WEDPS KFL M LKA+ ++ + S DLFV FN D ETV+LP P +G++W
Sbjct: 750 PPKWEDPSCKFLAMTLKAERNEHQESSVSSDILGDLFVAFNADDCPETVVLPLPPEGMSW 809
Query: 352 FRLVDTALPFPEFFSTDGEPVIEQTTGLLAYVMKSHSCALFEA 224
+R++DTALPFP FF +G+ V+EQ +GL Y MKS+SC LFEA
Sbjct: 810 YRIIDTALPFPGFFLNNGDLVLEQMSGLSTYEMKSYSCILFEA 852