BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00017438
         (317 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis ...    77   1e-12
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                    75   6e-12
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...    70   1e-10
dbj|BAH84784.1| cytochrome P450 [Nicotiana tabacum]                    68   7e-10
ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...    68   7e-10

>ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis vinifera]
          Length = 507

 Score = 77.0 bits (188), Expect = 1e-12
 Identities = 43/106 (40%), Positives = 60/106 (56%), Gaps = 1/106 (0%)
 Frame = -1

Query: 317 MRMVAGKRCFEE-CMGLDEQKRLHVHLKECFMPRQLLNMEDFLPILRWIGLYGREEKFVI 141
           M+ + GKRC EE   G++  ++    LK  F+P    N+ DF PILRW+G  G  EK VI
Sbjct: 185 MKTITGKRCIEEEAEGIETNRQFLERLKRIFVPTTTTNLCDFFPILRWVGYKGL-EKSVI 243

Query: 140 NLQKMRDSFLQEMIDDYRRKNIRSSTTSIAEEMSKTTILDALLSIQ 3
              K RD +LQ M+D++RR N      S  E   K T+++ LL +Q
Sbjct: 244 QFGKERDGYLQGMLDEFRRNN------SAVEWQKKRTLIETLLFLQ 283


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score = 75.1 bits (183), Expect = 6e-12
 Identities = 43/106 (40%), Positives = 66/106 (62%), Gaps = 1/106 (0%)
 Frame = -1

Query: 317 MRMVAGKRCF-EECMGLDEQKRLHVHLKECFMPRQLLNMEDFLPILRWIGLYGREEKFVI 141
           MRM+AGKR + E    L++ ++    ++E F      N+ DFLP+LRWIG  G E++ +I
Sbjct: 181 MRMIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLRWIGYKGIEKR-LI 239

Query: 140 NLQKMRDSFLQEMIDDYRRKNIRSSTTSIAEEMSKTTILDALLSIQ 3
            L+  RD FLQE+I +  R+ +R ++ S   E  K T++D LLS+Q
Sbjct: 240 RLKNKRDEFLQELIQE--RRKLRVNSKSFPSEEKKKTLIDVLLSLQ 283


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score = 70.5 bits (171), Expect = 1e-10
 Identities = 42/106 (39%), Positives = 63/106 (59%), Gaps = 1/106 (0%)
 Frame = -1

Query: 317 MRMVAGKRCFEECMGLDEQKRLHVHL-KECFMPRQLLNMEDFLPILRWIGLYGREEKFVI 141
           M+M+ GKR + E +G  E+ R+   +  E F      NM DFLPIL W+GL G E + +I
Sbjct: 179 MKMIGGKRYYGENIGEVEEARMFREMVSETFQLAGATNMVDFLPILGWLGLKGTERR-LI 237

Query: 140 NLQKMRDSFLQEMIDDYRRKNIRSSTTSIAEEMSKTTILDALLSIQ 3
            LQK R+SF+Q +I+++RRK           E  + T+++ LLS+Q
Sbjct: 238 KLQKRRESFIQNLIEEHRRKGSNC-------EGRQKTMIEVLLSLQ 276


>dbj|BAH84784.1| cytochrome P450 [Nicotiana tabacum]
          Length = 496

 Score = 68.2 bits (165), Expect = 7e-10
 Identities = 44/111 (39%), Positives = 67/111 (60%), Gaps = 6/111 (5%)
 Frame = -1

Query: 317 MRMVAGKRCF-EECMGLDEQKRLHVHLKECFMPRQL-LNMEDFLPILRWIGLYGREEKFV 144
           MR   GKRC  EE MG ++ K++   +K  F    + LN+ DF+P+L+W G  G E++ V
Sbjct: 187 MRTGTGKRCVSEEDMGTEKGKQIIEEIKGFFFATLVVLNVCDFMPVLKWFGYKGLEKRMV 246

Query: 143 INLQKMRDSFLQEMIDDYRRKN----IRSSTTSIAEEMSKTTILDALLSIQ 3
           +  QK R+ FL  ++D++R+K       SST SI     KTT+++ LLS+Q
Sbjct: 247 LAHQK-RNEFLNSLLDEFRQKKNSWYSESSTDSI--NAKKTTLVETLLSLQ 294


>ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 498

 Score = 68.2 bits (165), Expect = 7e-10
 Identities = 41/105 (39%), Positives = 61/105 (58%)
 Frame = -1

Query: 317 MRMVAGKRCFEECMGLDEQKRLHVHLKECFMPRQLLNMEDFLPILRWIGLYGREEKFVIN 138
           MRM+ GKR + E +G  E+ R    +          NM DFLPIL W+GL G E++ ++ 
Sbjct: 179 MRMIGGKRYYGENVGEVEEARKFREMVSKAFRLAGTNMVDFLPILGWLGLKGTEKR-LME 237

Query: 137 LQKMRDSFLQEMIDDYRRKNIRSSTTSIAEEMSKTTILDALLSIQ 3
           LQKMRDSF+Q +I+++R K  +        E    T+++ LLS+Q
Sbjct: 238 LQKMRDSFIQNLIEEHRIKGSKC-------ERRPKTMIEVLLSLQ 275