BLASTX nr result
ID: Coptis23_contig00016996
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00016996
(1059 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002324218.1| predicted protein [Populus trichocarpa] gi|2... 343 e-125
ref|XP_004162977.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 332 e-122
ref|XP_004148391.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 332 e-122
dbj|BAJ94465.1| predicted protein [Hordeum vulgare subsp. vulgar... 345 e-121
ref|XP_003522715.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 335 e-120
>ref|XP_002324218.1| predicted protein [Populus trichocarpa] gi|222865652|gb|EEF02783.1|
predicted protein [Populus trichocarpa]
Length = 561
Score = 343 bits (881), Expect(2) = e-125
Identities = 168/269 (62%), Positives = 197/269 (73%)
Frame = +1
Query: 253 YGAGVIIFLGFNQENSLVPRFLEGFLELGSPDSLRDESSIDSKKPLKVCSVKAGGMMSLA 432
+G + LG + EN +P ++ FLELGS SL D S S+ PLK+CS+KAGGMMSLA
Sbjct: 89 WGYNIYGQLGVSGENYSMPHLMDQFLELGSLGSLIDHSETKSEGPLKICSIKAGGMMSLA 148
Query: 433 IDSLGALWIWGNYPPQSHSGDGEFSHISSSAPLPVWEFHGHTVVKVACGNEHVVALVSAE 612
ID GALW+WGN P Q + S SS P+PV +FHGH+VVKVACG+EHVVALVSA
Sbjct: 149 IDDHGALWMWGNCPQQRNKTGSSISFESSFIPIPVRDFHGHSVVKVACGSEHVVALVSAG 208
Query: 613 ETFTGGDLICYSWGNNNHRQLGLGDRENRLRPEIVKTFNRECPWEVYEVACGAFHTAVLT 792
ET+ DL+CYSWGNNNH QLGLGDRE+R+ PEIV+ FN++ W+VYEVACGA+HTA+LT
Sbjct: 209 ETYKVEDLVCYSWGNNNHGQLGLGDRESRVHPEIVEMFNKDSSWDVYEVACGAYHTALLT 268
Query: 793 RKKIHGNMIDSSHLESLELGEKESICWTFGLGDNGQLGHGTTNSTYLPEPVKDLPPDVFL 972
RK+ + ES+CWTFGLGDNGQLGHGTT S PEPVK+LP L
Sbjct: 269 RKR-------------RTVDPLESVCWTFGLGDNGQLGHGTTQSVLKPEPVKELPGQAHL 315
Query: 973 ISVDCGLFHTSVVSSAGDVWSWGMEKGLG 1059
ISVDCGLFHTSVVSS GDVWSWGMEKGLG
Sbjct: 316 ISVDCGLFHTSVVSSVGDVWSWGMEKGLG 344
Score = 131 bits (329), Expect(2) = e-125
Identities = 62/88 (70%), Positives = 72/88 (81%)
Frame = +3
Query: 12 LSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEFDNKAQGDRP 191
LS KV+S+A GEAH+L LTGDG VYSWG+G FGRLGTG E DELFPV+V+F+ + R
Sbjct: 7 LSGKVVSVATGEAHSLILTGDGRVYSWGKGMFGRLGTGSEVDELFPVQVKFETCVE-KRL 65
Query: 192 KFVEIAAGAYHSLALQDDGSIWCWGYNI 275
FV +AAGAYHSLAL DDGS+WCWGYNI
Sbjct: 66 NFVGVAAGAYHSLALADDGSVWCWGYNI 93
>ref|XP_004162977.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Cucumis sativus]
Length = 581
Score = 332 bits (850), Expect(2) = e-122
Identities = 159/270 (58%), Positives = 195/270 (72%), Gaps = 1/270 (0%)
Frame = +1
Query: 253 YGAGVIIFLGFNQENSLVPRFLEGFLELGSPDSLRDESSIDSKKPLKVCSVKAGGMMSLA 432
+G + LGF+ NS P L+ FLEL P+SL +ES SK LK+C +KAGG +S A
Sbjct: 94 WGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKICDIKAGGQISFA 153
Query: 433 IDSLGALWIWGNYPPQSHSGDGEFSHISSSAPLPVWEFHGHTVVKVACGNEHVVALVSAE 612
ID LGALW+WGN P S + S++SS P PVWEFHGHTVVKVACGNEH+VALVS
Sbjct: 154 IDDLGALWMWGNCPKPSGENEVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVG 213
Query: 613 ETFT-GGDLICYSWGNNNHRQLGLGDRENRLRPEIVKTFNRECPWEVYEVACGAFHTAVL 789
ET+ DL+CYSWG+N+H QLGLGD+++R P+I++TFN PW VYEVACGAFHTA+L
Sbjct: 214 ETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALL 273
Query: 790 TRKKIHGNMIDSSHLESLELGEKESICWTFGLGDNGQLGHGTTNSTYLPEPVKDLPPDVF 969
+ K + + ES+CWTFGLG+NGQLGHGTT + +LP+PVK+LP V
Sbjct: 274 SHSKRPCDTL-------------ESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVS 320
Query: 970 LISVDCGLFHTSVVSSAGDVWSWGMEKGLG 1059
LISV CGLFHTSVVSS GD+WSWGME+GLG
Sbjct: 321 LISVACGLFHTSVVSSGGDLWSWGMERGLG 350
Score = 134 bits (337), Expect(2) = e-122
Identities = 64/90 (71%), Positives = 73/90 (81%)
Frame = +3
Query: 6 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEFDNKAQGD 185
GKLS KV++++AGEAHTL LTGDG VYSWGRG FGRLGTG E DELFPVRV+ + +
Sbjct: 11 GKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGE--E 68
Query: 186 RPKFVEIAAGAYHSLALQDDGSIWCWGYNI 275
K V IAAG+YHSLAL DDGS+WCWGYNI
Sbjct: 69 NLKVVAIAAGSYHSLALTDDGSVWCWGYNI 98
Score = 59.7 bits (143), Expect = 1e-06
Identities = 37/100 (37%), Positives = 55/100 (55%), Gaps = 13/100 (13%)
Frame = +3
Query: 6 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTG----------KEDDELFP-- 149
G+ + KV+++AAG H+LALT DG V+ WG +G+LG K+ EL P
Sbjct: 66 GEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPE 125
Query: 150 -VRVEFDNKAQGDRPKFVEIAAGAYHSLALQDDGSIWCWG 266
+ E +K++G K +I AG S A+ D G++W WG
Sbjct: 126 SLTEESGSKSKGSL-KICDIKAGGQISFAIDDLGALWMWG 164
>ref|XP_004148391.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Cucumis sativus]
Length = 571
Score = 332 bits (850), Expect(2) = e-122
Identities = 159/270 (58%), Positives = 195/270 (72%), Gaps = 1/270 (0%)
Frame = +1
Query: 253 YGAGVIIFLGFNQENSLVPRFLEGFLELGSPDSLRDESSIDSKKPLKVCSVKAGGMMSLA 432
+G + LGF+ NS P L+ FLEL P+SL +ES SK LK+C +KAGG +S A
Sbjct: 94 WGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKICDIKAGGQISFA 153
Query: 433 IDSLGALWIWGNYPPQSHSGDGEFSHISSSAPLPVWEFHGHTVVKVACGNEHVVALVSAE 612
ID LGALW+WGN P S + S++SS P PVWEFHGHTVVKVACGNEH+VALVS
Sbjct: 154 IDDLGALWMWGNCPKPSGENEVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVG 213
Query: 613 ETFT-GGDLICYSWGNNNHRQLGLGDRENRLRPEIVKTFNRECPWEVYEVACGAFHTAVL 789
ET+ DL+CYSWG+N+H QLGLGD+++R P+I++TFN PW VYEVACGAFHTA+L
Sbjct: 214 ETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALL 273
Query: 790 TRKKIHGNMIDSSHLESLELGEKESICWTFGLGDNGQLGHGTTNSTYLPEPVKDLPPDVF 969
+ K + + ES+CWTFGLG+NGQLGHGTT + +LP+PVK+LP V
Sbjct: 274 SHSKRPCDTL-------------ESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVS 320
Query: 970 LISVDCGLFHTSVVSSAGDVWSWGMEKGLG 1059
LISV CGLFHTSVVSS GD+WSWGME+GLG
Sbjct: 321 LISVACGLFHTSVVSSGGDLWSWGMERGLG 350
Score = 134 bits (337), Expect(2) = e-122
Identities = 64/90 (71%), Positives = 73/90 (81%)
Frame = +3
Query: 6 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEFDNKAQGD 185
GKLS KV++++AGEAHTL LTGDG VYSWGRG FGRLGTG E DELFPVRV+ + +
Sbjct: 11 GKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGE--E 68
Query: 186 RPKFVEIAAGAYHSLALQDDGSIWCWGYNI 275
K V IAAG+YHSLAL DDGS+WCWGYNI
Sbjct: 69 NLKVVAIAAGSYHSLALTDDGSVWCWGYNI 98
Score = 59.7 bits (143), Expect = 1e-06
Identities = 37/100 (37%), Positives = 55/100 (55%), Gaps = 13/100 (13%)
Frame = +3
Query: 6 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTG----------KEDDELFP-- 149
G+ + KV+++AAG H+LALT DG V+ WG +G+LG K+ EL P
Sbjct: 66 GEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPE 125
Query: 150 -VRVEFDNKAQGDRPKFVEIAAGAYHSLALQDDGSIWCWG 266
+ E +K++G K +I AG S A+ D G++W WG
Sbjct: 126 SLTEESGSKSKGSL-KICDIKAGGQISFAIDDLGALWMWG 164
>dbj|BAJ94465.1| predicted protein [Hordeum vulgare subsp. vulgare]
gi|326532960|dbj|BAJ89325.1| predicted protein [Hordeum
vulgare subsp. vulgare]
Length = 566
Score = 345 bits (886), Expect(2) = e-121
Identities = 168/270 (62%), Positives = 203/270 (75%), Gaps = 1/270 (0%)
Frame = +1
Query: 253 YGAGVIIFLGFNQENSLVPRFLEGFLELGSPDSLRDESSID-SKKPLKVCSVKAGGMMSL 429
+G + LG+ +ENSL P ++GF +LGSP++L+DE+ ++ PLK+ SVKAGGMMS
Sbjct: 87 WGYNIYGQLGYGEENSLSPCLVDGFQDLGSPETLQDEAQDTLARTPLKLSSVKAGGMMSF 146
Query: 430 AIDSLGALWIWGNYPPQSHSGDGEFSHISSSAPLPVWEFHGHTVVKVACGNEHVVALVSA 609
AIDSLGALW+WGN P Q+ DGEF ++SAPLPVW+FHGHTVVKVACGNEHVVA VSA
Sbjct: 147 AIDSLGALWMWGNCPQQTD--DGEFYIAATSAPLPVWDFHGHTVVKVACGNEHVVAAVSA 204
Query: 610 EETFTGGDLICYSWGNNNHRQLGLGDRENRLRPEIVKTFNRECPWEVYEVACGAFHTAVL 789
ET+TGGDL+CYSWGNNNH QLGLGD+E+R RP ++ F WEVYE+ACGA+HTAVL
Sbjct: 205 GETYTGGDLVCYSWGNNNHGQLGLGDKESRSRPMLIPEFGEGSSWEVYEIACGAWHTAVL 264
Query: 790 TRKKIHGNMIDSSHLESLELGEKESICWTFGLGDNGQLGHGTTNSTYLPEPVKDLPPDVF 969
T KK + ES CWTFG+GDNGQLGHGTT +T P+PV LP F
Sbjct: 265 TNKKSFDQ-------------DLESRCWTFGIGDNGQLGHGTTATTCSPQPVDGLPTGSF 311
Query: 970 LISVDCGLFHTSVVSSAGDVWSWGMEKGLG 1059
LIS+DCGLFHT+VVSS G+VW WGME+GLG
Sbjct: 312 LISLDCGLFHTAVVSSDGEVWCWGMERGLG 341
Score = 117 bits (292), Expect(2) = e-121
Identities = 59/88 (67%), Positives = 66/88 (75%), Gaps = 3/88 (3%)
Frame = +3
Query: 21 KVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRV---EFDNKAQGDRP 191
KV ++AAGEAHTLALTGDG VYSWGRGTFGRLGTG+E D P V A RP
Sbjct: 4 KVSAVAAGEAHTLALTGDGEVYSWGRGTFGRLGTGREADVHVPTAVLPAVAAAAAGRPRP 63
Query: 192 KFVEIAAGAYHSLALQDDGSIWCWGYNI 275
+FV +AAGAYHSLAL D+GS+W WGYNI
Sbjct: 64 RFVAVAAGAYHSLALDDEGSLWSWGYNI 91
>ref|XP_003522715.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Glycine max]
Length = 559
Score = 335 bits (860), Expect(2) = e-120
Identities = 170/257 (66%), Positives = 192/257 (74%), Gaps = 1/257 (0%)
Frame = +1
Query: 292 ENSLVPRFLEGFLELGSPDSLRDE-SSIDSKKPLKVCSVKAGGMMSLAIDSLGALWIWGN 468
+NSLVPR L FLEL PDS S +SK PLK+C+VKAGGMMSLAID+ G LW+WGN
Sbjct: 124 DNSLVPRILNKFLELHPPDSSSSGVSEAESKTPLKICAVKAGGMMSLAIDNHGTLWMWGN 183
Query: 469 YPPQSHSGDGEFSHISSSAPLPVWEFHGHTVVKVACGNEHVVALVSAEETFTGGDLICYS 648
P QS +G + +SS P PVW+F GHTVVKVACGNEHVVALVSA ET+ G DL+CYS
Sbjct: 184 CPRQSK--EGGLALVSSFTPTPVWDFQGHTVVKVACGNEHVVALVSAGETYNGEDLVCYS 241
Query: 649 WGNNNHRQLGLGDRENRLRPEIVKTFNRECPWEVYEVACGAFHTAVLTRKKIHGNMIDSS 828
WG N+ QLGLGDRE+RL PE+V+TF+ E P YEVACGAFHTA+LTRKK + +
Sbjct: 242 WGYNSRGQLGLGDRESRLHPEVVRTFDEESP---YEVACGAFHTALLTRKKKPSDTV--- 295
Query: 829 HLESLELGEKESICWTFGLGDNGQLGHGTTNSTYLPEPVKDLPPDVFLISVDCGLFHTSV 1008
ES CWTFGLGDNGQLGHGTT ST P PVK+LP +V LI VDCGLFHTSV
Sbjct: 296 ----------ESTCWTFGLGDNGQLGHGTTQSTLFPTPVKELPQNVSLICVDCGLFHTSV 345
Query: 1009 VSSAGDVWSWGMEKGLG 1059
VSS GDVWSWGMEKGLG
Sbjct: 346 VSSDGDVWSWGMEKGLG 362
Score = 122 bits (307), Expect(2) = e-120
Identities = 59/93 (63%), Positives = 71/93 (76%), Gaps = 4/93 (4%)
Frame = +3
Query: 6 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEF----DNK 173
G++S+KV+ +AAGEAHTL LTGDG VY WG+G FGRLGTG E DEL PV++ F N
Sbjct: 12 GQVSRKVVDVAAGEAHTLLLTGDGSVYCWGKGMFGRLGTGAEKDELLPVQLNFGYPNPNG 71
Query: 174 AQGDRPKFVEIAAGAYHSLALQDDGSIWCWGYN 272
+G K V IAAGAYHSLAL +DG++WCWGYN
Sbjct: 72 TEGTF-KIVGIAAGAYHSLALAEDGAVWCWGYN 103