BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00015565
         (1252 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...   318   1e-84
dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       317   4e-84
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   316   7e-84
ref|XP_002285061.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   313   4e-83
emb|CAN60309.1| hypothetical protein VITISV_015004 [Vitis vinifera]   311   3e-82

>ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 498

 Score =  318 bits (816), Expect = 1e-84
 Identities = 171/350 (48%), Positives = 230/350 (65%)
 Frame = -1

Query: 1222 VELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQAKAKEKLMDLKSTFCVTGKFGLGDFFP 1043
            VE++S F +L  N+IMR+IG + +  E+  VG+ +   K  ++ S         + DF P
Sbjct: 164  VEMKSAFFELTLNVIMRMIGGKRYYGEN--VGEVEEARKFREMVSKAFRLAGTNMVDFLP 221

Query: 1042 FLKWVDIWRVEKIMMKLFKKRDKFLHGLINDCRTRTNSSFSASTTREYRKEEKDNKSIID 863
             L W+ +   EK +M+L K RD F+  LI + R + +            K E+  K++I+
Sbjct: 222  ILGWLGLKGTEKRLMELQKMRDSFIQNLIEEHRIKGS------------KCERRPKTMIE 269

Query: 862  VLLSLQETQPEYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLNHPEVLNKARTEIEC 683
            VLLSLQET+PE YTD+II G++  ML AGT+ S  T EWA+SLLLN PEVL KA+ EI+ 
Sbjct: 270  VLLSLQETEPENYTDEIIGGLMLSMLTAGTDTSAGTMEWALSLLLNSPEVLKKAQQEIDV 329

Query: 682  NVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCTIGGYNIPKGTLLFV 503
            ++G+DRL+ E DL +LPYL +II ETLR+Y  GP   PHES+ +C +GG+ IP GT+L V
Sbjct: 330  HLGHDRLIEEVDLAQLPYLRSIIKETLRMYPAGPLLIPHESSKECFVGGFRIPPGTMLLV 389

Query: 502  NFLALHRDPKAWSEPTIFKPERFEKDRKGNEGLQWMPFGVGRRGCPGEGFAKRMLGLVLG 323
            N  A+H DPK W+EPT FKPERFE +    +GL+++PFG GRRGCPGEG A RM+GL +G
Sbjct: 390  NVWAIHNDPKIWAEPTKFKPERFEGEEGERDGLRFLPFGSGRRGCPGEGLAIRMVGLAMG 449

Query: 322  ILIQCFEWEQVGGEMVDMTTKMERGFILKAMPLEAMYRPSKPMFNVLSRL 173
             LIQCF+WE+V  +MVDMT       I KA PL A  RP   M N+LS+L
Sbjct: 450  SLIQCFDWERVDQQMVDMTEGHGLS-IPKAQPLLAKCRPRPTMVNLLSQL 498


>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  317 bits (812), Expect = 4e-84
 Identities = 166/359 (46%), Positives = 233/359 (64%), Gaps = 2/359 (0%)
 Frame = -1

Query: 1246 GYNENFTKVELRSIFCKLIFNMIMRII-GMQPFLREDEMVGQAKAKEKLMDLKSTFCVTG 1070
            G    +  VE++++  +L  N++M +I G + +  + E + QAK  ++++  + T  ++G
Sbjct: 157  GQGNEYRTVEMKTMIFELTLNVMMTMIAGKRYYGGQVEELEQAKRFQEIV--QGTLAISG 214

Query: 1069 KFGLGDFFPFLKWVDIWRVEKIMMKLFKKRDKFLHGLINDCRTRTNSSFSASTTREYRKE 890
               +GDF P L W+ I  +EK +  L +KRDKFL  LI + R   +              
Sbjct: 215  ASNIGDFLPILSWIGIKGIEKKVWNLKEKRDKFLQELIEEHRISRSGGMQT--------- 265

Query: 889  EKDNKSIIDVLLSLQETQPEYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLNHPEVL 710
            ++ N ++IDVLLSLQET+PEYYTD++I G+I V+L AGT+ S  T EWAMSLLLN+P+V+
Sbjct: 266  KEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVI 325

Query: 709  NKARTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCTIGGYN 530
             KA+ EI+ N+   RL+NE+D++KLPYL +II ETLR+Y  GP   PHES+++C +GGY 
Sbjct: 326  KKAQAEIDNNLEQGRLINESDVNKLPYLHSIITETLRIYPAGPLLVPHESSEECIVGGYK 385

Query: 529  IPKGTLLFVNFLALHRDPKAWSEPTIFKPERFEKDRKGNEGLQWMPFGVGRRGCPGEGFA 350
            +P GT+L VN  A+ +DP  W EPT FKPERF+      +G + MPFG GRRGCPGEG A
Sbjct: 386  VPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLA 445

Query: 349  KRMLGLVLGILIQCFEWEQVGGEMVDMTTKMERGFIL-KAMPLEAMYRPSKPMFNVLSR 176
             R++ L LG LIQCF+WE+VG EMVDM+     G  L K  PLEA  RP     N +S+
Sbjct: 446  MRVVALALGALIQCFDWERVGEEMVDMSE--GPGLTLPKVHPLEAKCRPRSTALNFISQ 502


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  316 bits (810), Expect = 7e-84
 Identities = 172/357 (48%), Positives = 238/357 (66%), Gaps = 4/357 (1%)
 Frame = -1

Query: 1231 FTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQAKAKEKLMDL-KSTFCVTGKFGLG 1055
            + KVE++S+F +L  N++MR+I  + +  E   V   +   K  ++ + TF ++G   +G
Sbjct: 163  YHKVEMKSVFFELTLNIMMRMIAGKRYYGEH--VADLEQARKFKEIVEETFELSGASNVG 220

Query: 1054 DFFPFLKWVDIWRVEKIMMKLFKKRDKFLHGLINDCRT-RTNSSFSASTTREYRKEEKDN 878
            DF P L+W+    +EK +++L  KRD+FL  LI + R  R NS       + +  EEK  
Sbjct: 221  DFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELIQERRKLRVNS-------KSFPSEEK-K 272

Query: 877  KSIIDVLLSLQETQPEYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLNHPEVLNKAR 698
            K++IDVLLSLQET+PEYYTD++I+G+I ++  AGT+ S  T EWAMSLLLN+P+++ KA+
Sbjct: 273  KTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQ 332

Query: 697  TEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCTIGGYNIPKG 518
            +EI+  +   R ++E+DL+KLPYL  IINETLR+Y  GP   PHES++DC IGGYNIP G
Sbjct: 333  SEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCG 392

Query: 517  TLLFVNFLALHRDPKAWSEPTIFKPERFEKDRKG-NEGLQWMPFGVGRRGCPGEGFAKRM 341
            T+L VN  A+  DP  W EP  FKPERFE  + G  +G + MPFG GRRGCPGEG A R+
Sbjct: 393  TMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFGSGRRGCPGEGLAXRV 452

Query: 340  LGLVLGILIQCFEWEQVGGEMVDMTTKMERGFIL-KAMPLEAMYRPSKPMFNVLSRL 173
            +GL L  L+QCF+W +VG EMV+M+     G  L K  PLEA  RP   M N LS++
Sbjct: 453  VGLTLXSLLQCFDWHRVGEEMVEMSE--GTGLTLPKLHPLEAHCRPRSTMLNFLSQV 507


>ref|XP_002285061.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 508

 Score =  313 bits (803), Expect = 4e-83
 Identities = 164/357 (45%), Positives = 225/357 (63%), Gaps = 2/357 (0%)
 Frame = -1

Query: 1237 ENFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQAKAKEKLMDL-KSTFCVTGKFG 1061
            + F +VE++S   +L FN+I RI+  + +   D  V   +   +  D+ K     +G   
Sbjct: 167  QGFARVEMKSRLSELSFNIITRIVAGKRYFGTD--VEDFEEASRFRDIIKEILETSGASN 224

Query: 1060 LGDFFPFLKWVDIWRVEKIMMKLFKKRDKFLHGLINDCRTRTNSSFSASTTREYRKEEKD 881
             GDF PFL+W D   ++K ++ L ++ D FL GLI++ R   NS         YR+E   
Sbjct: 225  AGDFLPFLQWFDFQGLKKRVLALQRRTDAFLQGLIDEGRNHNNS---------YRQERGK 275

Query: 880  NKSIIDVLLSLQETQPEYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLNHPEVLNKA 701
             K+ +D +L+LQ ++PEYY D IIKG+I  +L AGT+ S  T EWAMSLLLNHP VL+K 
Sbjct: 276  TKTFVDSMLALQNSEPEYYADHIIKGMILTLLTAGTDTSAVTMEWAMSLLLNHPTVLDKV 335

Query: 700  RTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCTIGGYNIPK 521
            +TE++C +G+ RL+ E DL  LPYL  I+NETLRL+   P    HES+DDC+IGGY++  
Sbjct: 336  KTELDCKIGHQRLVEEPDLSDLPYLRAIVNETLRLFPAAPLLVAHESSDDCSIGGYDVRG 395

Query: 520  GTLLFVNFLALHRDPKAWSEPTIFKPERFEKDRKGNEGLQWMPFGVGRRGCPGEGFAKRM 341
            GT+L VN  A+HRD K W +PT F+PERFE      E  +++PFG+GRRGCPG G A R+
Sbjct: 396  GTMLLVNAWAIHRDAKVWEDPTSFRPERFEGGE--GEACRFIPFGLGRRGCPGAGLANRV 453

Query: 340  LGLVLGILIQCFEWEQVGGEMVDMTTKMERGFIL-KAMPLEAMYRPSKPMFNVLSRL 173
            +GL L  L+QCFEW++VG   VDM+    +G  + KA PLEAM R    M  VLS L
Sbjct: 454  MGLALAALVQCFEWQRVGEVEVDMSE--GKGLTMPKAQPLEAMCRARNSMIKVLSEL 508


>emb|CAN60309.1| hypothetical protein VITISV_015004 [Vitis vinifera]
          Length = 990

 Score =  311 bits (796), Expect = 3e-82
 Identities = 166/356 (46%), Positives = 230/356 (64%), Gaps = 1/356 (0%)
 Frame = -1

Query: 1237 ENFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQAKAKEKLMDLKSTFCVTGKFGL 1058
            ENF KVEL+S+F +L+ N+ MR++  + F   D M    +A+E     K      G    
Sbjct: 648  ENFAKVELKSMFSELLLNITMRMVAGKRFYG-DNMKDVEEAREFREISKEILEFXGTSNP 706

Query: 1057 GDFFPFLKWVDIWRVEKIMMKLFKKRDKFLHGLINDCRTRTNSSFSASTTREYRKEEKDN 878
            GDF P L+W+D     K  ++L KK D FL GL+++CR+   S              ++ 
Sbjct: 707  GDFXPILQWIDYQGYNKRALRLGKKMDVFLQGLLDECRSNKRSDL------------ENR 754

Query: 877  KSIIDVLLSLQETQPEYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLNHPEVLNKAR 698
             ++ID LLSLQE++PEYYTD+IIKG+I  M + G + +  T EWAMSLLLNHPEVL KAR
Sbjct: 755  NTMIDHLLSLQESEPEYYTDEIIKGLIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKAR 814

Query: 697  TEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCTIGGYNIPKG 518
             E++ ++G+D L++ETDL KL YL +II+E+LRL+   P   PH ST+DC +GG+++P G
Sbjct: 815  DELDTHIGHDCLIDETDLPKLQYLQSIISESLRLFPSTPLLVPHFSTEDCKLGGFDVPGG 874

Query: 517  TLLFVNFLALHRDPKAWSEPTIFKPERFEKDRKGNEGLQWMPFGVGRRGCPGEGFAKRML 338
            T+L VN  ALHRDPK W++PT FKPERFE     +E  + +PFGVGRR CPG G A R++
Sbjct: 875  TMLLVNAWALHRDPKLWNDPTSFKPERFETGE--SETYKLLPFGVGRRACPGIGLANRVM 932

Query: 337  GLVLGILIQCFEWEQVGGEMVDMTTKMERGFIL-KAMPLEAMYRPSKPMFNVLSRL 173
            GL LG LIQCF+W++V  + +DM     +G  + K  PLEAM +  + M NV S++
Sbjct: 933  GLTLGSLIQCFDWKRVDEKEIDMXE--GQGLTMPKVEPLEAMCKTRQVMNNVSSKI 986



 Score =  291 bits (744), Expect = 3e-76
 Identities = 157/343 (45%), Positives = 218/343 (63%), Gaps = 2/343 (0%)
 Frame = -1

Query: 1237 ENFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQAKAKEKLMDLKSTFCVTGKFGL 1058
            +NF KVEL+S+F +L FN+I R++  + +  E     +AK   ++  ++ +F ++     
Sbjct: 165  DNFAKVELKSLFSELTFNIITRMVAGKRYYGEGSDFEEAKHFREI--IRKSFLLSAASNP 222

Query: 1057 GDFFPFLKWVDIWRVEKIMMKLFKKRDKFLHGLINDCRTRTNSSFSASTTREYRKEEKDN 878
            GDF P L+W+D    EK M K  ++ D  L GLI++ R+ +            +K    N
Sbjct: 223  GDFLPILRWMDYGGYEKKMAKNSRELDVILQGLIDEHRSNS------------KKGLMGN 270

Query: 877  KSIIDVLLSLQETQPEYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLNHPEVLNKAR 698
             ++ID LLSLQ+++PEYYTD IIKGV   ++ AGT+ +  T EWAMSLLLNHP+VL KA+
Sbjct: 271  NTMIDHLLSLQKSEPEYYTDQIIKGVTMNLVFAGTDTAAVTMEWAMSLLLNHPDVLKKAK 330

Query: 697  TEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCTIGGYNIPKG 518
             E++  VG +RLL E DL KL YL NII+ET RL    P   PH S+ +C +GG++IP+ 
Sbjct: 331  VELDTXVGQERLLEEADLPKLHYLQNIISETFRLCPPAPLWLPHMSSANCQLGGFDIPRD 390

Query: 517  TLLFVNFLALHRDPKAWSEPTIFKPERFEKDRKGNEGLQWMPFGVGRRGCPGEGFAKRML 338
             +L VN   LHRDPK W +PT FKPERFE   +G E  + +PFG GRR CPG G A +++
Sbjct: 391  XMLLVNSWTLHRDPKLWDDPTSFKPERFEGGERG-ETYKLLPFGTGRRACPGSGLANKVV 449

Query: 337  GLVLGILIQCFEWEQVGGEMVDMTTKME-RGFILKAM-PLEAM 215
            GL LG LIQC+EWE++  + VDM   ME +G  +  M PLEAM
Sbjct: 450  GLTLGSLIQCYEWERISEKKVDM---MEGKGLTMPKMEPLEAM 489