BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00015309
         (309 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       194   5e-48
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   174   9e-42
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   172   3e-41
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       168   4e-40
gb|AGC29953.1| CYP719A23 [Sinopodophyllum hexandrum]                  145   3e-33

>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  194 bits (494), Expect = 5e-48
 Identities = 99/103 (96%), Positives = 100/103 (97%)
 Frame = -1

Query: 309 EELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHE 130
           EEL+RPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELF LGVDSTSSTATWALAYLIHE
Sbjct: 252 EELMRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFLLGVDSTSSTATWALAYLIHE 311

Query: 129 QAVQEKLYEDILMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK 1
           QAVQEKL EDI MTIGDVDLVKIEDLSKLKYLQAVVKETMRMK
Sbjct: 312 QAVQEKLNEDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK 354


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  174 bits (440), Expect = 9e-42
 Identities = 85/103 (82%), Positives = 94/103 (91%)
 Frame = -1

Query: 309 EELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHE 130
           E+LV PLLSSNPPTNSYLYFL SQ +S+E+IIFC FEL+ LGVDSTSST TWALAYLI E
Sbjct: 253 EDLVSPLLSSNPPTNSYLYFLLSQRFSEEVIIFCIFELYLLGVDSTSSTTTWALAYLIRE 312

Query: 129 QAVQEKLYEDILMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK 1
           QA+QEKLY+D  MT+GDVDLVKIED+SKLKYLQAVVKETMRMK
Sbjct: 313 QAIQEKLYQDTRMTLGDVDLVKIEDVSKLKYLQAVVKETMRMK 355


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  172 bits (436), Expect = 3e-41
 Identities = 84/103 (81%), Positives = 92/103 (89%)
 Frame = -1

Query: 309 EELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHE 130
           EELVRPLLSS PPTNSYLYFL SQN+ +E+IIFC FEL+ LGVDSTSST TWALAYLI E
Sbjct: 252 EELVRPLLSSKPPTNSYLYFLLSQNFEEEVIIFCIFELYLLGVDSTSSTTTWALAYLIRE 311

Query: 129 QAVQEKLYEDILMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK 1
           Q  QEKLY+DI MT+GDVDLVKIED++KLKYLQ VVKETMRMK
Sbjct: 312 QGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMK 354


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  168 bits (426), Expect = 4e-40
 Identities = 83/103 (80%), Positives = 91/103 (88%)
 Frame = -1

Query: 309 EELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHE 130
           EELVRPLLSS PPTN+YLYFL SQN S+E+ IFC FELF LGVDS SST TWALAY+I E
Sbjct: 268 EELVRPLLSSKPPTNAYLYFLLSQNLSEEVTIFCIFELFLLGVDSASSTTTWALAYMIRE 327

Query: 129 QAVQEKLYEDILMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK 1
           Q  QEKLY+DI MTIGDVDLVKIED++KLKYLQ+VVKETMRMK
Sbjct: 328 QEAQEKLYQDIRMTIGDVDLVKIEDVNKLKYLQSVVKETMRMK 370


>gb|AGC29953.1| CYP719A23 [Sinopodophyllum hexandrum]
          Length = 489

 Score =  145 bits (367), Expect = 3e-33
 Identities = 75/105 (71%), Positives = 91/105 (86%), Gaps = 2/105 (1%)
 Frame = -1

Query: 309 EELVRPLLSS-NPPTNSYLYFLQSQNYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIH 133
           ++LVRP+++S NPP+NSYL FLQSQNYS+EIII C FEL+ L +DS++STATWALA++I 
Sbjct: 251 DDLVRPVVASANPPSNSYLGFLQSQNYSEEIIIACIFELYLLAMDSSASTATWALAFMIR 310

Query: 132 EQAVQEKLYEDILMTIGD-VDLVKIEDLSKLKYLQAVVKETMRMK 1
           +Q VQEKLY+DI   IGD VDLVK EDLSK+ YLQAVVKETMRMK
Sbjct: 311 DQQVQEKLYQDIKRVIGDGVDLVKAEDLSKMHYLQAVVKETMRMK 355