BLASTX nr result
ID: Coptis23_contig00013910
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00013910
(1631 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002284797.2| PREDICTED: tRNA pseudouridine synthase A, mi... 606 e-171
ref|XP_003610360.1| tRNA pseudouridine synthase [Medicago trunca... 606 e-171
ref|XP_003610356.1| tRNA pseudouridine synthase [Medicago trunca... 606 e-171
gb|ACJ85562.1| unknown [Medicago truncatula] gi|388491044|gb|AFK... 603 e-170
ref|XP_002300673.1| predicted protein [Populus trichocarpa] gi|2... 590 e-166
>ref|XP_002284797.2| PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Vitis
vinifera]
Length = 503
Score = 606 bits (1563), Expect = e-171
Identities = 313/490 (63%), Positives = 363/490 (74%), Gaps = 46/490 (9%)
Frame = +3
Query: 78 LKMSTTTSDDEEQTINNPNQKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEAL 257
+ +TTTSDDE+ +KPRYKRRKIAIF AYCGVGYQGMQKNPGAKTIEG+LEEAL
Sbjct: 13 MSQTTTTSDDEDCDTTTATKKPRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEGDLEEAL 72
Query: 258 YLSGAVPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYVDPPGFIDRLNSNLPNQIR 437
+ SGAVPE DRG PKRFDWARSARTDKGVSAVGQVVSGRFY+DPPG ++RLNSNLP QIR
Sbjct: 73 FFSGAVPEQDRGHPKRFDWARSARTDKGVSAVGQVVSGRFYIDPPGLVERLNSNLPPQIR 132
Query: 438 IFGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSE 617
IFGF RVT+SF+AKKFCDRRRYVYLIPVFALD ++H DRESVLAS+GS+NELVKCLECSE
Sbjct: 133 IFGFKRVTASFNAKKFCDRRRYVYLIPVFALDPSSHPDRESVLASVGSENELVKCLECSE 192
Query: 618 RGRKVLGAMGKHYSGPKI---EDNKAXXXXXXXXXXXXXXXXXXXXXMASVE---CGNG- 776
RGRKV+G MGK K+ + MAS + GN
Sbjct: 193 RGRKVVGIMGKRSFESKVSVDDREDVEGESKNIVGAAILKSEAKEESMASSDNRHDGNSI 252
Query: 777 --------------------DEKGVLENESGFS-------------------YGEKEKER 839
D + +NE G S YG+KE+ER
Sbjct: 253 SQPIKEGRCFQDNETSESTPDSRASAQNEPGVSHSEQSDKGENKLEKGSGFCYGDKERER 312
Query: 840 FNRIFSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANGVVTVDGMDFVKCEVVGQSFML 1019
FNRI HYVGTHNFHNFTTRIKAEDPSA RFI+SF+ N +VTV+G++FVKCEVVGQSFML
Sbjct: 313 FNRILGHYVGTHNFHNFTTRIKAEDPSAQRFIVSFDGNTIVTVEGIEFVKCEVVGQSFML 372
Query: 1020 HQIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFTSYNQKWKDT 1199
HQIRK++GLAVAIMR+ APESL+ETAL KDV INVPTAPEVGLYL+ECFF+SYN+KW+D+
Sbjct: 373 HQIRKMIGLAVAIMRNVAPESLLETALLKDVNINVPTAPEVGLYLDECFFSSYNKKWRDS 432
Query: 1200 HEEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVEDNETCT 1379
HEE+SM+AY E+AE+FKMK VYSHIA+ E KEG VA+WLHSLN RNYPDL + DN +
Sbjct: 433 HEEVSMKAYAEEAEEFKMKHVYSHIAATEHKEGVVALWLHSLNHRNYPDL-CIGDNGAAS 491
Query: 1380 IGENVQVESI 1409
G+N + E++
Sbjct: 492 DGKNGEEETM 501
>ref|XP_003610360.1| tRNA pseudouridine synthase [Medicago truncatula]
gi|355511415|gb|AES92557.1| tRNA pseudouridine synthase
[Medicago truncatula] gi|388510220|gb|AFK43176.1| unknown
[Medicago truncatula]
Length = 530
Score = 606 bits (1562), Expect = e-171
Identities = 319/494 (64%), Positives = 359/494 (72%), Gaps = 52/494 (10%)
Frame = +3
Query: 78 LKMSTTTSDDEEQTINNPNQKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEAL 257
+KMSTTTSDDE T K RYKRRK+AIF AYCGVGYQGMQKNPGAKTIEGELEEAL
Sbjct: 29 VKMSTTTSDDEGCTTAE-GSKVRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEGELEEAL 87
Query: 258 YLSGAVPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYVDPPGFIDRLNSNLPNQIR 437
Y+SGAVPE DRG KR+DWARSARTDKGVSAVGQVVSGRFY+DPPGF+DRLNSNLP+QIR
Sbjct: 88 YVSGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLPSQIR 147
Query: 438 IFGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSE 617
IFGF RVT+SFSAKKFCDRRRYVYLIPVFALD HRDRE+VLASLGS+NELVKCL CSE
Sbjct: 148 IFGFKRVTASFSAKKFCDRRRYVYLIPVFALDPCCHRDRETVLASLGSENELVKCLPCSE 207
Query: 618 RGRKVLGAMG-----------------------------------------KHYSGPKIE 674
RGRKV G +G H + I
Sbjct: 208 RGRKVEGVVGNSKRNLELEAVDVENGSSNKNDVVDSGVTKDVEVSLSKGDDNHLNKESIN 267
Query: 675 DNKAXXXXXXXXXXXXXXXXXXXXXMASVECGNGDEKGVLENE-----------SGFSYG 821
DN+ +A + G+ + G+LE E SGF YG
Sbjct: 268 DNEG---KVSVEEVNSKTVVSDQDEVAPINGGSENNLGILEEEKVNREDTAANGSGFCYG 324
Query: 822 EKEKERFNRIFSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANGVVTVDGMDFVKCEVV 1001
EKE+ERFN I YVGTHNFHNFTTRIK EDPSA RFIISF+A+ V V+GM+FVKCE+V
Sbjct: 325 EKERERFNNILKCYVGTHNFHNFTTRIKPEDPSAKRFIISFDASTTVVVEGMEFVKCEIV 384
Query: 1002 GQSFMLHQIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFTSYN 1181
GQSFMLHQIRK++GLAVAIMR+CAPESLIE ALQKDV I VPTAPEVGLYL+ECFFTSYN
Sbjct: 385 GQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLYLDECFFTSYN 444
Query: 1182 QKWKDTHEEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVE 1361
QKWKDTHEE+SM+AY ++AEDFKM+ +Y HIAS E KEG+V +WLHSLN RNYPDLR +
Sbjct: 445 QKWKDTHEEVSMKAYEKEAEDFKMQYIYPHIASTEYKEGTVGLWLHSLNHRNYPDLRIL- 503
Query: 1362 DNETCTIGENVQVE 1403
D E T + V+V+
Sbjct: 504 DGEGVTNDKKVEVD 517
>ref|XP_003610356.1| tRNA pseudouridine synthase [Medicago truncatula]
gi|355511411|gb|AES92553.1| tRNA pseudouridine synthase
[Medicago truncatula]
Length = 530
Score = 606 bits (1562), Expect = e-171
Identities = 319/494 (64%), Positives = 359/494 (72%), Gaps = 52/494 (10%)
Frame = +3
Query: 78 LKMSTTTSDDEEQTINNPNQKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEAL 257
+KMSTTTSDDE T K RYKRRK+AIF AYCGVGYQGMQKNPGAKTIEGELEEAL
Sbjct: 29 VKMSTTTSDDEGCTTAE-GSKVRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEGELEEAL 87
Query: 258 YLSGAVPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYVDPPGFIDRLNSNLPNQIR 437
Y+SGAVPE DRG KR+DWARSARTDKGVSAVGQVVSGRFY+DPPGF+DRLNSNLP+QIR
Sbjct: 88 YVSGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLPSQIR 147
Query: 438 IFGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSE 617
IFGF RVT+SFSAKKFCDRRRYVYLIPVFALD HRDRE+VLASLGS+NELVKCL CSE
Sbjct: 148 IFGFKRVTASFSAKKFCDRRRYVYLIPVFALDPCCHRDRETVLASLGSENELVKCLPCSE 207
Query: 618 RGRKVLGAMG-----------------------------------------KHYSGPKIE 674
RGRKV G +G H + I
Sbjct: 208 RGRKVEGVVGNSKRNLELEAVDVENGSSNKNDVVDSGVTKDVEVSLSKGDDNHLNKESIN 267
Query: 675 DNKAXXXXXXXXXXXXXXXXXXXXXMASVECGNGDEKGVLENE-----------SGFSYG 821
DN+ +A + G+ + G+LE E SGF YG
Sbjct: 268 DNEG---KVSVEEVNSKTVVSDEDEVAPINGGSENNLGILEEEKVNREDTAANGSGFCYG 324
Query: 822 EKEKERFNRIFSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANGVVTVDGMDFVKCEVV 1001
EKE+ERFN I YVGTHNFHNFTTRIK EDPSA RFIISF+A+ V V+GM+FVKCE+V
Sbjct: 325 EKERERFNNILKCYVGTHNFHNFTTRIKPEDPSAKRFIISFDASTTVVVEGMEFVKCEIV 384
Query: 1002 GQSFMLHQIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFTSYN 1181
GQSFMLHQIRK++GLAVAIMR+CAPESLIE ALQKDV I VPTAPEVGLYL+ECFFTSYN
Sbjct: 385 GQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLYLDECFFTSYN 444
Query: 1182 QKWKDTHEEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVE 1361
QKWKDTHEE+SM+AY ++AEDFKM+ +Y HIAS E KEG+V +WLHSLN RNYPDLR +
Sbjct: 445 QKWKDTHEEVSMKAYEKEAEDFKMQYIYPHIASTEYKEGTVGLWLHSLNHRNYPDLRIL- 503
Query: 1362 DNETCTIGENVQVE 1403
D E T + V+V+
Sbjct: 504 DGEGVTNDKKVEVD 517
>gb|ACJ85562.1| unknown [Medicago truncatula] gi|388491044|gb|AFK33588.1| unknown
[Medicago truncatula]
Length = 530
Score = 603 bits (1555), Expect = e-170
Identities = 318/494 (64%), Positives = 358/494 (72%), Gaps = 52/494 (10%)
Frame = +3
Query: 78 LKMSTTTSDDEEQTINNPNQKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEAL 257
+KMSTTTSDDE T K RYKRRK+AIF AYCGVGYQGMQKNPGAKTIEGELEEAL
Sbjct: 29 VKMSTTTSDDEGCTTAE-GSKVRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEGELEEAL 87
Query: 258 YLSGAVPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYVDPPGFIDRLNSNLPNQIR 437
Y+SGAVPE DRG KR+DWARSARTDKGVSAVGQVVSGRFY+DPPGF+DRLNSNLP+QIR
Sbjct: 88 YVSGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLPSQIR 147
Query: 438 IFGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSE 617
IFGF RVT+SFSAKKFCDRRRYVYLIPVFALD HRDRE+VLASLGS+NELVKCL CSE
Sbjct: 148 IFGFKRVTASFSAKKFCDRRRYVYLIPVFALDPCCHRDRETVLASLGSENELVKCLPCSE 207
Query: 618 RGRKVLGAMG-----------------------------------------KHYSGPKIE 674
RGRKV G +G H + I
Sbjct: 208 RGRKVEGVVGNSKRNLELEAVDVENGSSNKNDVVDSGVTKDVEVSLSKGDDNHLNKESIN 267
Query: 675 DNKAXXXXXXXXXXXXXXXXXXXXXMASVECGNGDEKGVLENE-----------SGFSYG 821
DN+ +A + G+ + G+LE E SGF YG
Sbjct: 268 DNEG---KVSVEEVNSKTVVSDQDEVAPINGGSENNLGILEEEKVNREDTAANGSGFCYG 324
Query: 822 EKEKERFNRIFSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANGVVTVDGMDFVKCEVV 1001
EKE+ERFN I YVGTHNFHNFTTRIK EDPSA RFIISF+A+ V V+GM+FVKCE+V
Sbjct: 325 EKERERFNNILKCYVGTHNFHNFTTRIKPEDPSAKRFIISFDASTTVVVEGMEFVKCEIV 384
Query: 1002 GQSFMLHQIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFTSYN 1181
GQSFMLHQIRK++GLAVAIMR+CAPESLIE ALQKDV I VPTAPEVGLYL+ECFFTSYN
Sbjct: 385 GQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLYLDECFFTSYN 444
Query: 1182 QKWKDTHEEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVE 1361
QKWKDTHEE+SM+AY ++AEDFKM+ +Y HIAS E KEG+V +WLHSLN RNYPDL +
Sbjct: 445 QKWKDTHEEVSMKAYEKEAEDFKMQYIYPHIASTEYKEGTVGLWLHSLNHRNYPDLGIL- 503
Query: 1362 DNETCTIGENVQVE 1403
D E T + V+V+
Sbjct: 504 DGEGVTNDKKVEVD 517
>ref|XP_002300673.1| predicted protein [Populus trichocarpa] gi|222842399|gb|EEE79946.1|
predicted protein [Populus trichocarpa]
Length = 429
Score = 590 bits (1522), Expect = e-166
Identities = 298/411 (72%), Positives = 338/411 (82%), Gaps = 4/411 (0%)
Frame = +3
Query: 135 QKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEALYLSGAVPEHDRGKPKRFDW 314
+K RYKRRKIAIF AYCGVGYQGMQKNPGAKTIEG+LEEAL+ +GAVPE DRG PKR+DW
Sbjct: 10 KKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEGDLEEALFHAGAVPEQDRGIPKRYDW 69
Query: 315 ARSARTDKGVSAVGQVVSGRFYVDPPGFIDRLNSNLPNQIRIFGFNRVTSSFSAKKFCDR 494
ARSARTDKGVSAVGQVVSGRFY+DPPG ++RL+SNL Q RIFG+ RVT SF+AKKFCDR
Sbjct: 70 ARSARTDKGVSAVGQVVSGRFYIDPPGLVERLHSNLSPQFRIFGYKRVTGSFNAKKFCDR 129
Query: 495 RRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSERGRKVLGAMGKHYSGPKIE 674
RRYVYLIPVFALD +HRDRESVLASL S +EL+KCLECSERGRKV GA+GK ++
Sbjct: 130 RRYVYLIPVFALDPCSHRDRESVLASLRSGSELIKCLECSERGRKVAGAVGKRSF--ELT 187
Query: 675 DNKAXXXXXXXXXXXXXXXXXXXXXMASVECGNGDEKGVLENE----SGFSYGEKEKERF 842
DN + + SVE G+ DEK + E S F YGEKEKERF
Sbjct: 188 DNSSNDKDASVKSEIKEEVDEI---IVSVENGD-DEKTKSKEEMVKGSSFCYGEKEKERF 243
Query: 843 NRIFSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANGVVTVDGMDFVKCEVVGQSFMLH 1022
NRI +YVG+HNFHNFTTR KAEDPSA R+I+SF A V V+G++FVKCEVVGQSFMLH
Sbjct: 244 NRILGYYVGSHNFHNFTTRTKAEDPSARRYIVSFEAKTTVNVEGIEFVKCEVVGQSFMLH 303
Query: 1023 QIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFTSYNQKWKDTH 1202
QIRK++G+AVAIMR+CAPESLI+TALQKDV INVPTAPEVGLYL+ECFFTSYNQKWKD+H
Sbjct: 304 QIRKMIGVAVAIMRNCAPESLIQTALQKDVNINVPTAPEVGLYLDECFFTSYNQKWKDSH 363
Query: 1203 EEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRF 1355
EEISM+ Y E+AEDFKMK +YSHIA+ E KEGSVA+WLHSLN RNYPDLRF
Sbjct: 364 EEISMKDYEEEAEDFKMKHIYSHIATTEHKEGSVALWLHSLNHRNYPDLRF 414