BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00013587
         (1732 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281380.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   681   0.0  
ref|XP_003554958.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   672   0.0  
ref|XP_003554957.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   672   0.0  
ref|XP_002306060.1| predicted protein [Populus trichocarpa] gi|2...   669   0.0  
ref|XP_004141471.1| PREDICTED: probable tRNA (guanine(26)-N(2))-...   663   0.0  

>ref|XP_002281380.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 2 [Vitis vinifera]
            gi|147815608|emb|CAN65976.1| hypothetical protein
            VITISV_036159 [Vitis vinifera]
            gi|298204459|emb|CBI16939.3| unnamed protein product
            [Vitis vinifera]
          Length = 579

 Score =  681 bits (1756), Expect(2) = 0.0
 Identities = 326/370 (88%), Positives = 350/370 (94%)
 Frame = -3

Query: 1394 DPYGSPSIFLDSAVQSVADGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 1215
            DPYGSPS+FLDSAVQ VADGGMLMCTATDMAVLCGGNGE CYSKYGSYPLRGKYCHEMAL
Sbjct: 207  DPYGSPSVFLDSAVQCVADGGMLMCTATDMAVLCGGNGEACYSKYGSYPLRGKYCHEMAL 266

Query: 1214 RILLTCIESHANRYKRYIVPVLSVQMDFYVRVFVRIFTSAGAMKNTPLKLSYVYQCIGCD 1035
            RILL  IESHANRYKRYIVPVLSVQMDFYVRVFVRI+TSA AMK+TPLKLSYVYQC GCD
Sbjct: 267  RILLASIESHANRYKRYIVPVLSVQMDFYVRVFVRIYTSASAMKSTPLKLSYVYQCTGCD 326

Query: 1034 SFHLQCVARTVTKNNSVRYLPGFGPVVPQECSDCGKKYNVGGPIWSAPIHDQEWVSSILA 855
            SFHLQ + RTV+KNNSVRYLPGFGPVVPQECSDCGKK+N+GGPIWSAPIHDQEWV+SILA
Sbjct: 327  SFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKKFNMGGPIWSAPIHDQEWVTSILA 386

Query: 854  NVISMKDRYPAFDRISAVLTTVSEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVINAG 675
            +V SMKDRYPA++RISAVLTT+SEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAV+NAG
Sbjct: 387  DVKSMKDRYPAYNRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVLNAG 446

Query: 674  YRISGTHVNPLGLKSDAPMNVIWDIMRCWVKNHPVKAQAEDQSGTVILAKEPTLQADFAR 495
            YRISGTHVNPLG+KSDAPM+VIWDIMRCWVKNHPVKAQ  DQSG+VILAKEP LQA+FAR
Sbjct: 447  YRISGTHVNPLGMKSDAPMDVIWDIMRCWVKNHPVKAQQPDQSGSVILAKEPVLQANFAR 506

Query: 494  AVASLSKAQAKKVARFLPNPEKHWGPKLRAGRQITSKHVSLLGPLAINGSEGHEEEDKVD 315
            AVASLSKAQ KKVARFLPNPE+HWGPKLRAGRQITSKHVSLLGP A+NGS  H+E+++ +
Sbjct: 507  AVASLSKAQTKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGPEAVNGSMNHQEDEEPN 566

Query: 314  SKRQKIDDPA 285
            SKRQK D  A
Sbjct: 567  SKRQKTDQAA 576



 Score =  134 bits (337), Expect(2) = 0.0
 Identities = 64/74 (86%), Positives = 71/74 (95%)
 Frame = -2

Query: 1662 SGLRALRYAREVEGLGQVIALDNDKASVEACKRNIKYNGSVACSKVEAHLADARVFMLTH 1483
            SGLRALRYAREVEG+GQV+ALDNDK SVEAC+RNIK+NGSVA SKV++ LADARV+MLTH
Sbjct: 138  SGLRALRYAREVEGVGQVVALDNDKVSVEACRRNIKFNGSVASSKVDSQLADARVYMLTH 197

Query: 1482 PKEFDVVDLDPYGS 1441
            PKEFDVVDLDPYGS
Sbjct: 198  PKEFDVVDLDPYGS 211


>ref|XP_003554958.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like isoform 2 [Glycine max]
          Length = 645

 Score =  672 bits (1735), Expect(2) = 0.0
 Identities = 320/372 (86%), Positives = 348/372 (93%)
 Frame = -3

Query: 1394 DPYGSPSIFLDSAVQSVADGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 1215
            DPYGSPS+FLDSAVQSV DGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL
Sbjct: 274  DPYGSPSMFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 333

Query: 1214 RILLTCIESHANRYKRYIVPVLSVQMDFYVRVFVRIFTSAGAMKNTPLKLSYVYQCIGCD 1035
            RI+L CIESHANRYKRYIVPVLSVQMDFY+RVFVRI+TSA AMKNTPLKLSYVYQC GCD
Sbjct: 334  RIVLACIESHANRYKRYIVPVLSVQMDFYLRVFVRIYTSASAMKNTPLKLSYVYQCTGCD 393

Query: 1034 SFHLQCVARTVTKNNSVRYLPGFGPVVPQECSDCGKKYNVGGPIWSAPIHDQEWVSSILA 855
            SFHLQ + RT++KN SVRYLPGFGPVVPQEC DCGKK+N+GGPIWSAPIHDQEWV++ILA
Sbjct: 394  SFHLQPIGRTISKNTSVRYLPGFGPVVPQECRDCGKKFNMGGPIWSAPIHDQEWVAAILA 453

Query: 854  NVISMKDRYPAFDRISAVLTTVSEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVINAG 675
            +V SMKDRYPA+DRISAVLTT+SEELPDVPLFLSLHNLCATLKCTSPSA+IFRSAVINAG
Sbjct: 454  DVKSMKDRYPAYDRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAIIFRSAVINAG 513

Query: 674  YRISGTHVNPLGLKSDAPMNVIWDIMRCWVKNHPVKAQAEDQSGTVILAKEPTLQADFAR 495
            YRISGTHVNPLGLKSDAPM+VIWDIMRCWVKNHPVK Q  DQ G++ILAKEP LQA+FAR
Sbjct: 514  YRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQPADQPGSIILAKEPVLQANFAR 573

Query: 494  AVASLSKAQAKKVARFLPNPEKHWGPKLRAGRQITSKHVSLLGPLAINGSEGHEEEDKVD 315
            AVASLSKAQAKKVARFLPNPE+HWGPKLRAGRQITSKHVSLLGP AING+   E++++  
Sbjct: 574  AVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGPEAINGALNQEDDEEPK 633

Query: 314  SKRQKIDDPASS 279
            SK+ K +D  +S
Sbjct: 634  SKKPKTEDSVTS 645



 Score =  135 bits (341), Expect(2) = 0.0
 Identities = 65/74 (87%), Positives = 72/74 (97%)
 Frame = -2

Query: 1662 SGLRALRYAREVEGLGQVIALDNDKASVEACKRNIKYNGSVACSKVEAHLADARVFMLTH 1483
            SGLR+LRYAREVEG+GQVIA+DNDKASVEAC+RNIK+NGSVA SKVE+HLADARV+MLTH
Sbjct: 205  SGLRSLRYAREVEGIGQVIAVDNDKASVEACRRNIKFNGSVAASKVESHLADARVYMLTH 264

Query: 1482 PKEFDVVDLDPYGS 1441
             KEFDVVDLDPYGS
Sbjct: 265  AKEFDVVDLDPYGS 278


>ref|XP_003554957.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like isoform 1 [Glycine max]
          Length = 638

 Score =  672 bits (1735), Expect(2) = 0.0
 Identities = 320/372 (86%), Positives = 348/372 (93%)
 Frame = -3

Query: 1394 DPYGSPSIFLDSAVQSVADGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 1215
            DPYGSPS+FLDSAVQSV DGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL
Sbjct: 267  DPYGSPSMFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 326

Query: 1214 RILLTCIESHANRYKRYIVPVLSVQMDFYVRVFVRIFTSAGAMKNTPLKLSYVYQCIGCD 1035
            RI+L CIESHANRYKRYIVPVLSVQMDFY+RVFVRI+TSA AMKNTPLKLSYVYQC GCD
Sbjct: 327  RIVLACIESHANRYKRYIVPVLSVQMDFYLRVFVRIYTSASAMKNTPLKLSYVYQCTGCD 386

Query: 1034 SFHLQCVARTVTKNNSVRYLPGFGPVVPQECSDCGKKYNVGGPIWSAPIHDQEWVSSILA 855
            SFHLQ + RT++KN SVRYLPGFGPVVPQEC DCGKK+N+GGPIWSAPIHDQEWV++ILA
Sbjct: 387  SFHLQPIGRTISKNTSVRYLPGFGPVVPQECRDCGKKFNMGGPIWSAPIHDQEWVAAILA 446

Query: 854  NVISMKDRYPAFDRISAVLTTVSEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVINAG 675
            +V SMKDRYPA+DRISAVLTT+SEELPDVPLFLSLHNLCATLKCTSPSA+IFRSAVINAG
Sbjct: 447  DVKSMKDRYPAYDRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAIIFRSAVINAG 506

Query: 674  YRISGTHVNPLGLKSDAPMNVIWDIMRCWVKNHPVKAQAEDQSGTVILAKEPTLQADFAR 495
            YRISGTHVNPLGLKSDAPM+VIWDIMRCWVKNHPVK Q  DQ G++ILAKEP LQA+FAR
Sbjct: 507  YRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKPQPADQPGSIILAKEPVLQANFAR 566

Query: 494  AVASLSKAQAKKVARFLPNPEKHWGPKLRAGRQITSKHVSLLGPLAINGSEGHEEEDKVD 315
            AVASLSKAQAKKVARFLPNPE+HWGPKLRAGRQITSKHVSLLGP AING+   E++++  
Sbjct: 567  AVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITSKHVSLLGPEAINGALNQEDDEEPK 626

Query: 314  SKRQKIDDPASS 279
            SK+ K +D  +S
Sbjct: 627  SKKPKTEDSVTS 638



 Score =  135 bits (341), Expect(2) = 0.0
 Identities = 65/74 (87%), Positives = 72/74 (97%)
 Frame = -2

Query: 1662 SGLRALRYAREVEGLGQVIALDNDKASVEACKRNIKYNGSVACSKVEAHLADARVFMLTH 1483
            SGLR+LRYAREVEG+GQVIA+DNDKASVEAC+RNIK+NGSVA SKVE+HLADARV+MLTH
Sbjct: 198  SGLRSLRYAREVEGIGQVIAVDNDKASVEACRRNIKFNGSVAASKVESHLADARVYMLTH 257

Query: 1482 PKEFDVVDLDPYGS 1441
             KEFDVVDLDPYGS
Sbjct: 258  AKEFDVVDLDPYGS 271


>ref|XP_002306060.1| predicted protein [Populus trichocarpa] gi|222849024|gb|EEE86571.1|
            predicted protein [Populus trichocarpa]
          Length = 582

 Score =  669 bits (1727), Expect(2) = 0.0
 Identities = 321/375 (85%), Positives = 348/375 (92%), Gaps = 3/375 (0%)
 Frame = -3

Query: 1394 DPYGSPSIFLDSAVQSVADGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 1215
            DPYGSPS+FLDSAVQSV DGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL
Sbjct: 207  DPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 266

Query: 1214 RILLTCIESHANRYKRYIVPVLSVQMDFYVRVFVRIFTSAGAMKNTPLKLSYVYQCIGCD 1035
            RILL  IESHANRYKRYIVPVLSVQM+FYVRVFVR++TSA AMKNTPLKLSYVYQCIGCD
Sbjct: 267  RILLASIESHANRYKRYIVPVLSVQMNFYVRVFVRVYTSASAMKNTPLKLSYVYQCIGCD 326

Query: 1034 SFHLQCVARTVTKNNSVRYLPGFGPVVPQECSDCGKKYNVGGPIWSAPIHDQEWVSSILA 855
            S+HLQ + RT++K +SVR+LPGFGPVVPQECSDCGKKYN+GGPIWSAPIHDQEWV+S+L 
Sbjct: 327  SYHLQPIGRTMSKGSSVRHLPGFGPVVPQECSDCGKKYNMGGPIWSAPIHDQEWVTSVLD 386

Query: 854  NVISMKDRYPAFDRISAVLTTVSEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVINAG 675
             V SMKDRYPA+DRISAVLTT+SEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVINAG
Sbjct: 387  GVKSMKDRYPAYDRISAVLTTISEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVINAG 446

Query: 674  YRISGTHVNPLGLKSDAPMNVIWDIMRCWVKNHPVKAQAEDQSGTVILAKEPTLQADFAR 495
            YRISGTHVNPLGLKSDAPM+VIWDIMRCWVKNHPVKAQA DQ G++ILAKEP LQA+FAR
Sbjct: 447  YRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQAPDQPGSIILAKEPVLQANFAR 506

Query: 494  AVASLSKAQAKKVARFLPNPEKHWGPKLRAGRQITSKHVSLLGPLAINGSEGHEEEDKVD 315
            AVASLSKAQAKKVARFLPNPE+HWGPKLRAGRQIT+KH+SLLGP A+NG   HE  ++  
Sbjct: 507  AVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITTKHISLLGPEAVNGHLNHENSEETK 566

Query: 314  SKRQK---IDDPASS 279
            +KR K    +DP SS
Sbjct: 567  AKRPKTGDTNDPTSS 581



 Score =  137 bits (345), Expect(2) = 0.0
 Identities = 65/74 (87%), Positives = 73/74 (98%)
 Frame = -2

Query: 1662 SGLRALRYAREVEGLGQVIALDNDKASVEACKRNIKYNGSVACSKVEAHLADARVFMLTH 1483
            SGLRALRYAREVEG+G+V+ALDNDKASVEAC++NIK+NGSVA SKVE+HLADARV+MLTH
Sbjct: 138  SGLRALRYAREVEGIGKVVALDNDKASVEACQKNIKFNGSVAISKVESHLADARVYMLTH 197

Query: 1482 PKEFDVVDLDPYGS 1441
            PKEFDVVDLDPYGS
Sbjct: 198  PKEFDVVDLDPYGS 211


>ref|XP_004141471.1| PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase
            2-like [Cucumis sativus]
          Length = 581

 Score =  663 bits (1710), Expect(2) = 0.0
 Identities = 314/368 (85%), Positives = 345/368 (93%)
 Frame = -3

Query: 1394 DPYGSPSIFLDSAVQSVADGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 1215
            DPYGSPSIFLDSAVQSV DGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL
Sbjct: 208  DPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMAL 267

Query: 1214 RILLTCIESHANRYKRYIVPVLSVQMDFYVRVFVRIFTSAGAMKNTPLKLSYVYQCIGCD 1035
            RILL CIESHANRYKRYIVPVLSVQMDFYVRVFVR++TSA AMKNTPLKLSYVYQC GCD
Sbjct: 268  RILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSASAMKNTPLKLSYVYQCTGCD 327

Query: 1034 SFHLQCVARTVTKNNSVRYLPGFGPVVPQECSDCGKKYNVGGPIWSAPIHDQEWVSSILA 855
            SFHLQ + R+V+KNNSVRYLPGFGP V QECSDCGKK+N+GGPIWSAPIHDQEWV+S+LA
Sbjct: 328  SFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSDCGKKFNMGGPIWSAPIHDQEWVTSMLA 387

Query: 854  NVISMKDRYPAFDRISAVLTTVSEELPDVPLFLSLHNLCATLKCTSPSAVIFRSAVINAG 675
            +V SMKD YPA+DRISAVLTT+SEEL DVPLFLSLHNLCATLKCTSPSAV FRSAVINAG
Sbjct: 388  DVKSMKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAG 447

Query: 674  YRISGTHVNPLGLKSDAPMNVIWDIMRCWVKNHPVKAQAEDQSGTVILAKEPTLQADFAR 495
            YRISGTHVNPLGLKSDAPM+VIWDIMRCWVKNHPVK Q EDQ+G++ILAKEP L+A+FAR
Sbjct: 448  YRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKTQPEDQAGSIILAKEPVLEANFAR 507

Query: 494  AVASLSKAQAKKVARFLPNPEKHWGPKLRAGRQITSKHVSLLGPLAINGSEGHEEEDKVD 315
            AVASLSKAQAKK+ARFLPNPE+HWGPKLRAGRQITSKH+SLLG  A+NG+  H + ++ +
Sbjct: 508  AVASLSKAQAKKIARFLPNPERHWGPKLRAGRQITSKHISLLGAEAVNGALSHTDSEEPN 567

Query: 314  SKRQKIDD 291
            +KR K+++
Sbjct: 568  AKRSKVEE 575



 Score =  141 bits (355), Expect(2) = 0.0
 Identities = 66/74 (89%), Positives = 73/74 (98%)
 Frame = -2

Query: 1662 SGLRALRYAREVEGLGQVIALDNDKASVEACKRNIKYNGSVACSKVEAHLADARVFMLTH 1483
            SGLRALRYAREVEG+GQV+ALDNDKASVEAC+RNIK+NGSVACSKVE+HL DARV+MLTH
Sbjct: 139  SGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACSKVESHLTDARVYMLTH 198

Query: 1482 PKEFDVVDLDPYGS 1441
            PKEFD+VDLDPYGS
Sbjct: 199  PKEFDMVDLDPYGS 212