BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00013185
         (1434 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002266132.2| PREDICTED: LOW QUALITY PROTEIN: lipoyl synth...   548   e-153
ref|XP_004136815.1| PREDICTED: lipoyl synthase, mitochondrial-li...   547   e-153
ref|NP_179682.1| lipoic acid synthase 1 [Arabidopsis thaliana] g...   546   e-153
gb|AFK48146.1| unknown [Medicago truncatula]                          546   e-153
sp|Q3LSN4.1|LIAS1_PEA RecName: Full=Lipoyl synthase 1, mitochond...   545   e-152

>ref|XP_002266132.2| PREDICTED: LOW QUALITY PROTEIN: lipoyl synthase, mitochondrial [Vitis
            vinifera] gi|308191441|sp|A5CB81.1|LIAS_VITVI RecName:
            Full=Lipoyl synthase, mitochondrial; AltName:
            Full=Lipoate synthase; Short=LS; Short=Lip-syn; AltName:
            Full=Lipoic acid synthase; Flags: Precursor
            gi|147825263|emb|CAN73265.1| hypothetical protein
            VITISV_021769 [Vitis vinifera]
          Length = 393

 Score =  548 bits (1413), Expect = e-153
 Identities = 265/311 (85%), Positives = 284/311 (91%)
 Frame = +3

Query: 288  ESYSVEVGTXXXXXXXXXWMKESIPGGEKYGKIKKKLRELNLHTVCEEAKCPNLGECWSG 467
            + YSVEVGT         WMKESIPGG KY +IKKKLR+LNLHTVCEEA+CPN+GECWSG
Sbjct: 70   DDYSVEVGTKKKPLSKPKWMKESIPGGAKYTQIKKKLRQLNLHTVCEEARCPNMGECWSG 129

Query: 468  GETGTATATIMILGDTCTRGCRFCNVKTSRNPLPPDPNEPSNVAEAIASWGLDYIVITSV 647
            GETGTATATIMILGDTCTRGCRFCNVKTSR P PPDP+EPS VAEAIASWGLDY+VITSV
Sbjct: 130  GETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPSKVAEAIASWGLDYVVITSV 189

Query: 648  DRDDLPDQGSAHFVETVQKLKILKPDILIEALVPDFRGDRSCVEKVSQSGLDVLAHNIET 827
            DRDDLPDQGS HF ETVQKLKILKP++LIEALVPDFRGD  CVEKVS+SGLDV AHNIET
Sbjct: 190  DRDDLPDQGSGHFAETVQKLKILKPNMLIEALVPDFRGDPGCVEKVSKSGLDVFAHNIET 249

Query: 828  VEELQGVVRDHRANFKQSMEVLKMAKDFAPVGTLTKTSIMLGCGETPDQVVRTMEKVRDA 1007
            VEELQ  VRDHRANFKQS+EVLK+AK++A  GTLTKTSIMLGCGETPDQVVRTMEKVR A
Sbjct: 250  VEELQSAVRDHRANFKQSLEVLKLAKEYADAGTLTKTSIMLGCGETPDQVVRTMEKVRAA 309

Query: 1008 GVDVMTFGQYMRPSKRHMPVSEYITPEAFEQYRILGTEMGFRYIASGPMVRSSYKAGEFY 1187
            GVDVMTFGQYMRPSKRHMPVSEYITPEAFE+YRILG +MGFRY+ASGPMVRSSYKAGEFY
Sbjct: 310  GVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYRILGMDMGFRYVASGPMVRSSYKAGEFY 369

Query: 1188 IKSMIEKDRAI 1220
            IKSMI+ DRA+
Sbjct: 370  IKSMIDADRAM 380


>ref|XP_004136815.1| PREDICTED: lipoyl synthase, mitochondrial-like [Cucumis sativus]
            gi|449479016|ref|XP_004155481.1| PREDICTED: lipoyl
            synthase, mitochondrial-like [Cucumis sativus]
          Length = 381

 Score =  547 bits (1409), Expect = e-153
 Identities = 264/310 (85%), Positives = 285/310 (91%)
 Frame = +3

Query: 288  ESYSVEVGTXXXXXXXXXWMKESIPGGEKYGKIKKKLRELNLHTVCEEAKCPNLGECWSG 467
            +SYSVEVGT         WM+ES+PGGEKY +IKKKLREL LHTVCEEAKCPNLGECWSG
Sbjct: 63   DSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSG 122

Query: 468  GETGTATATIMILGDTCTRGCRFCNVKTSRNPLPPDPNEPSNVAEAIASWGLDYIVITSV 647
            GETGTATATIMILGDTCTRGCRFCNVKTSR P PPDPNEP+NVAEAIASWGLDY+VITSV
Sbjct: 123  GETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSV 182

Query: 648  DRDDLPDQGSAHFVETVQKLKILKPDILIEALVPDFRGDRSCVEKVSQSGLDVLAHNIET 827
            DRDDLPDQGS HF ETVQKLK+LKP++LIEALVPDFRGD  CVEKV++SGLDV AHNIET
Sbjct: 183  DRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIET 242

Query: 828  VEELQGVVRDHRANFKQSMEVLKMAKDFAPVGTLTKTSIMLGCGETPDQVVRTMEKVRDA 1007
            VEELQGVVRDHRANFKQS++VL MAK+F+  GTLTKTSIMLGCGETPDQVV+TMEKVR A
Sbjct: 243  VEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVRAA 302

Query: 1008 GVDVMTFGQYMRPSKRHMPVSEYITPEAFEQYRILGTEMGFRYIASGPMVRSSYKAGEFY 1187
            GVDVMTFGQYMRPSKRHMPVSEYITPEAFE+Y+ +G +MGFRY+ASGPMVRSSYKAGEFY
Sbjct: 303  GVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGEFY 362

Query: 1188 IKSMIEKDRA 1217
            IKSMIE DRA
Sbjct: 363  IKSMIESDRA 372


>ref|NP_179682.1| lipoic acid synthase 1 [Arabidopsis thaliana]
            gi|75217181|sp|Q9ZWT1.1|LIAS_ARATH RecName: Full=Lipoyl
            synthase, mitochondrial; AltName: Full=Lipoate synthase;
            Short=LS; Short=Lip-syn; AltName: Full=Lipoic acid
            synthase; Flags: Precursor
            gi|15983418|gb|AAL11577.1|AF424583_1 At2g20860/F5H14.17
            [Arabidopsis thaliana] gi|3928758|dbj|BAA34701.1| Lipoic
            acid synthase [Arabidopsis thaliana]
            gi|4454462|gb|AAD20909.1| lipoic acid synthase (LIP1)
            [Arabidopsis thaliana] gi|14334940|gb|AAK59647.1|
            putative lipoic acid synthase LIP1 [Arabidopsis thaliana]
            gi|16323378|gb|AAL15183.1| putative lipoic acid synthase
            LIP1 [Arabidopsis thaliana] gi|330251994|gb|AEC07088.1|
            lipoic acid synthase 1 [Arabidopsis thaliana]
          Length = 374

 Score =  546 bits (1408), Expect = e-153
 Identities = 262/314 (83%), Positives = 288/314 (91%), Gaps = 1/314 (0%)
 Frame = +3

Query: 288  ESYSVEVGTXXXXXXXXXWMKESIPGGEKYGKIKKKLRELNLHTVCEEAKCPNLGECWSG 467
            ++YSVEVGT         WMKESIPGGE+Y +IKKKLR+L LHTVCEEAKCPNLGECWSG
Sbjct: 58   DTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECWSG 117

Query: 468  GETGTATATIMILGDTCTRGCRFCNVKTSRNPLPPDPNEPSNVAEAIASWGLDYIVITSV 647
            GETGTATATIMILGDTCTRGCRFCNVKTSR P PPDPNEP+NVAEAIASWG+DY+VITSV
Sbjct: 118  GETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVITSV 177

Query: 648  DRDDLPDQGSAHFVETVQKLKILKPDILIEALVPDFRGDRSCVEKVSQSGLDVLAHNIET 827
            DRDDLPDQGS HF ETVQ+LK LKP++LIEALVPDFRGD  CVEKVS+SGLDVLAHNIET
Sbjct: 178  DRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNIET 237

Query: 828  VEELQGVVRDHRANFKQSMEVLKMAKDFAPVGTLTKTSIMLGCGETPDQVVRTMEKVRDA 1007
            VEELQ  VRDHRANFKQS++VL+MAK++AP GTLTKTS+MLGCGETPDQVV+TMEKVR A
Sbjct: 238  VEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVRAA 297

Query: 1008 GVDVMTFGQYMRPSKRHMPVSEYITPEAFEQYRILGTEMGFRYIASGPMVRSSYKAGEFY 1187
            GVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+YR+LG EMGFRY+ASGPMVRSSYKAGE+Y
Sbjct: 298  GVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGEYY 357

Query: 1188 IKSMIEKDR-AIPS 1226
            IKSMIE DR A PS
Sbjct: 358  IKSMIEADRVASPS 371


>gb|AFK48146.1| unknown [Medicago truncatula]
          Length = 378

 Score =  546 bits (1406), Expect = e-153
 Identities = 263/312 (84%), Positives = 284/312 (91%)
 Frame = +3

Query: 291  SYSVEVGTXXXXXXXXXWMKESIPGGEKYGKIKKKLRELNLHTVCEEAKCPNLGECWSGG 470
            +YSVEVGT         WMKESIPGG KY +IKKKLREL LHTVCEEAKCPN+GECWSGG
Sbjct: 65   AYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECWSGG 124

Query: 471  ETGTATATIMILGDTCTRGCRFCNVKTSRNPLPPDPNEPSNVAEAIASWGLDYIVITSVD 650
            ETGTATATIMILGDTCTRGCRFCNVKTSR P PPDP+EP+NVAEAIASWGLDY+VITSVD
Sbjct: 125  ETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVITSVD 184

Query: 651  RDDLPDQGSAHFVETVQKLKILKPDILIEALVPDFRGDRSCVEKVSQSGLDVLAHNIETV 830
            RDDLPDQGS+HF ETVQKLKILKP ILIEALVPDFRG+  CVEKVS+SGLDV AH+IETV
Sbjct: 185  RDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAHSIETV 244

Query: 831  EELQGVVRDHRANFKQSMEVLKMAKDFAPVGTLTKTSIMLGCGETPDQVVRTMEKVRDAG 1010
            EELQ  VRDHRANF QS++VL+MAKD+AP GTLTKTSIMLGCGETPDQ+V+TMEKVR AG
Sbjct: 245  EELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEKVRAAG 304

Query: 1011 VDVMTFGQYMRPSKRHMPVSEYITPEAFEQYRILGTEMGFRYIASGPMVRSSYKAGEFYI 1190
            VDVMTFGQYMRPSKRHMPVSEYITPEAFE+Y+ LG EMGFRY+ASGPMVRSSYKAGEFYI
Sbjct: 305  VDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQALGMEMGFRYVASGPMVRSSYKAGEFYI 364

Query: 1191 KSMIEKDRAIPS 1226
            KSMI+ DRA+ S
Sbjct: 365  KSMIDSDRAVSS 376


>sp|Q3LSN4.1|LIAS1_PEA RecName: Full=Lipoyl synthase 1, mitochondrial; AltName: Full=Lipoate
            synthase 1; Short=LS 1; Short=Lip-syn 1; AltName:
            Full=Lipoic acid synthase 1; Flags: Precursor
            gi|75860378|gb|ABA29156.1| putative lipoic acid synthase
            [Pisum sativum]
          Length = 376

 Score =  545 bits (1404), Expect = e-152
 Identities = 264/312 (84%), Positives = 281/312 (90%)
 Frame = +3

Query: 291  SYSVEVGTXXXXXXXXXWMKESIPGGEKYGKIKKKLRELNLHTVCEEAKCPNLGECWSGG 470
            +YSVEVGT         WMKESIPGGEKY +IKKKLREL LHTVCEEAKCPNLGECWSGG
Sbjct: 65   AYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGG 124

Query: 471  ETGTATATIMILGDTCTRGCRFCNVKTSRNPLPPDPNEPSNVAEAIASWGLDYIVITSVD 650
            ETGTATATIMILGDTCTRGCRFCNVKTSR P PPDP+EP+NVAEAIASWGLDY+VITSVD
Sbjct: 125  ETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVITSVD 184

Query: 651  RDDLPDQGSAHFVETVQKLKILKPDILIEALVPDFRGDRSCVEKVSQSGLDVLAHNIETV 830
            RDDLPDQGS HF ETVQKLK LKP  LIEALVPDFRG+  CVEKVS+SGLDV AHNIETV
Sbjct: 185  RDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAECVEKVSKSGLDVFAHNIETV 244

Query: 831  EELQGVVRDHRANFKQSMEVLKMAKDFAPVGTLTKTSIMLGCGETPDQVVRTMEKVRDAG 1010
            EELQ  VRDHRANFKQS++VL MAK++AP GTLTKTSIMLGCGETPDQ+V+TMEKVR AG
Sbjct: 245  EELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVRAAG 304

Query: 1011 VDVMTFGQYMRPSKRHMPVSEYITPEAFEQYRILGTEMGFRYIASGPMVRSSYKAGEFYI 1190
            VDVMTFGQYMRPSKRHMPVSEYITPEAFE+Y+ LG EMGFRY+ASGPMVRSSYKAGEFYI
Sbjct: 305  VDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGEFYI 364

Query: 1191 KSMIEKDRAIPS 1226
            KSMI+ DRA  S
Sbjct: 365  KSMIDSDRAASS 376