BLASTX nr result
ID: Coptis23_contig00012651
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00012651
(4144 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum] 877 0.0
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum] 842 0.0
ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ... 840 0.0
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum] 836 0.0
gb|ADU15550.1| 4CL [Gossypium hirsutum] 836 0.0
>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
Length = 565
Score = 877 bits (2265), Expect = 0.0
Identities = 446/566 (78%), Positives = 491/566 (86%), Gaps = 3/566 (0%)
Frame = -3
Query: 3719 MITIASPETKPPQISPQVSTSETENTCPETFVFRSKLPDIPISNHLPLHAYCFERLSEFS 3540
M+++ASPET+P SP T EN E F+FRSKLPDIPISNHLPLH YCFE+++EFS
Sbjct: 1 MLSVASPETQPQIPSP---TPTPENPPVENFIFRSKLPDIPISNHLPLHTYCFEKITEFS 57
Query: 3539 DRPCLISGSDGRIYTYAETHLICQRTAVGLSRLGIKKGDVIMILLQNCPEFVFSFMGASM 3360
D PCLISGS GRIYT+AETHLICQ++A GLS+LGIKKGDVIMILLQN PEF FSFMGASM
Sbjct: 58 DAPCLISGSSGRIYTFAETHLICQKSAAGLSKLGIKKGDVIMILLQNSPEFAFSFMGASM 117
Query: 3359 LGAITTTANPFYTSAEIFKQVSASGAKLIITQSHYVDKLQHSNGISC---EDFKVVTIND 3189
+GAITTTANPF TSAEIFKQ S SGAKL+ITQS YVDKL+ + EDF V+T++D
Sbjct: 118 IGAITTTANPFCTSAEIFKQFSVSGAKLVITQSQYVDKLRDTGEKFPKIGEDFTVITVDD 177
Query: 3188 PQENCIHFSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISSVAQQV 3009
P E+C+HFS IS ADE +I VSIDP+D V++P+SSGTTGLPKGV LTHKSLISSVAQQV
Sbjct: 178 PPESCLHFSVISGADEREIPSVSIDPDDPVSMPFSSGTTGLPKGVFLTHKSLISSVAQQV 237
Query: 3008 DGENPNLYLTADDIVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNLIQKHR 2829
DGENPNLYL DDIVLCVLPLFHIYSLNSVLLCSLR GAGVLLM KFEI++LL LIQ+HR
Sbjct: 238 DGENPNLYLKTDDIVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMHKFEIRSLLELIQRHR 297
Query: 2828 VSXXXXXXXXXXXXAKDPIVDKFDLSSIRIVLSGAAPLGKELEDALRSRVPQAIFGQGYG 2649
VS AK+P+V FDLSSIRIVLSGAAPLGKELE+AL SR+PQAIFGQGYG
Sbjct: 298 VSVAPVVPPLVLALAKNPMVASFDLSSIRIVLSGAAPLGKELEEALHSRLPQAIFGQGYG 357
Query: 2648 MTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEICIRGPQ 2469
MTEAGPVLSMC GFA+Q FPTKSGSCGTVVRNAELK+IDPE+G SL+ NQPGEICIRGPQ
Sbjct: 358 MTEAGPVLSMCLGFARQAFPTKSGSCGTVVRNAELKVIDPETGFSLQYNQPGEICIRGPQ 417
Query: 2468 IMKGYLNDADATASTIDVEGWLHTGDIGSVDDDDEVFIVDRVKELIKFKGFQVPPAELEA 2289
IMKGYLNDA+ATASTIDV+GWLHTGD+G VDDDDEVFIVDRVKELIKFKGFQVPPAELEA
Sbjct: 418 IMKGYLNDAEATASTIDVDGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEA 477
Query: 2288 LLVSHPSIADAGVVPQMDAVAGEVPVAFIVPSNDFELTEEAVKEFIAKQVVFYKRLHKVY 2109
LL+SHPSIADA VVPQ D AGEVPVAF+V S+ FELTEEAVKEFIAKQVVFYKRLHKVY
Sbjct: 478 LLISHPSIADAAVVPQKDEAAGEVPVAFVVRSSGFELTEEAVKEFIAKQVVFYKRLHKVY 537
Query: 2108 FIHAIPKSPSGKILRKDLRAKLASSS 2031
F+HAIPKS SGKIL KDLRAKLA+ S
Sbjct: 538 FVHAIPKSASGKILGKDLRAKLATGS 563
>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
Length = 573
Score = 842 bits (2174), Expect = 0.0
Identities = 428/574 (74%), Positives = 481/574 (83%), Gaps = 8/574 (1%)
Frame = -3
Query: 3716 ITIASPETKPPQISPQVSTSETENTCPETFVFRSKLPDIPISNHLPLHAYCFERLSEFSD 3537
+ + SP KP Q+SP+ P +FRSKLPDIPISNHLPLH YCFE L SD
Sbjct: 9 VELESP--KPDQLSPET---------PADHIFRSKLPDIPISNHLPLHTYCFENLPSLSD 57
Query: 3536 RPCLISGSDGRIYTYAETHLICQRTAVGLSRLGIKKGDVIMILLQNCPEFVFSFMGASML 3357
+PCLISGS G+IY+++ETHLI Q+TA GLS LGI+KGD IMILL NC EFVF+FMGASM+
Sbjct: 58 KPCLISGSSGKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGASMI 117
Query: 3356 GAITTTANPFYTSAEIFKQVSASGAKLIITQSHYVDKLQHSNGISC--------EDFKVV 3201
GA++TTANPFYTS EIFKQ A+ AKLIITQS YVDKL+ +N EDFKV+
Sbjct: 118 GAVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFKVI 177
Query: 3200 TINDPQENCIHFSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISSV 3021
TI+DP ENC+ FS +S A+E +I V+I P+D VALP+SSGTTGLPKGV+LTHKSLI+SV
Sbjct: 178 TIDDPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSV 237
Query: 3020 AQQVDGENPNLYLTADDIVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNLI 2841
AQQVDGENPNLYLT DD+VLCVLPLFHIYSLNSVLLCSLR GA VLLMQKFEI TLL LI
Sbjct: 238 AQQVDGENPNLYLTHDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELI 297
Query: 2840 QKHRVSXXXXXXXXXXXXAKDPIVDKFDLSSIRIVLSGAAPLGKELEDALRSRVPQAIFG 2661
Q+H+VS AK+P+V +FDLSSIR+VLSGAAPLGKELEDALR RVPQA+ G
Sbjct: 298 QRHKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLG 357
Query: 2660 QGYGMTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEICI 2481
QGYGMTEAGPVLSMC GFAKQPFPTKSGSCGTVVRNAELK+IDPE+G SL NQPGEICI
Sbjct: 358 QGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICI 417
Query: 2480 RGPQIMKGYLNDADATASTIDVEGWLHTGDIGSVDDDDEVFIVDRVKELIKFKGFQVPPA 2301
RG QIMKGYLND ATA+TIDVEGWLHTGDIG VD+DDE+FIVDRVKE+IKFKGFQVPPA
Sbjct: 418 RGSQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPA 477
Query: 2300 ELEALLVSHPSIADAGVVPQMDAVAGEVPVAFIVPSNDFELTEEAVKEFIAKQVVFYKRL 2121
ELE+LL+SHPSIADA VVPQ D VAGEVPVAF+V SN FELTEEAVKEFIAKQVV+YKRL
Sbjct: 478 ELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVYYKRL 537
Query: 2120 HKVYFIHAIPKSPSGKILRKDLRAKLASSS*EKH 2019
HKV+F+HAIPKSPSGKILRKDLRAKLA+++ H
Sbjct: 538 HKVHFVHAIPKSPSGKILRKDLRAKLAAAAPNTH 571
>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
putative [Ricinus communis]
Length = 573
Score = 840 bits (2171), Expect = 0.0
Identities = 432/571 (75%), Positives = 488/571 (85%), Gaps = 8/571 (1%)
Frame = -3
Query: 3719 MITIASPETKPPQISPQVSTSETEN---TCPETFVFRSKLPDIPISNHLPLHAYCFERLS 3549
MI+IAS E ++S S+S+ +N + ET +FRSKLPDIPISNHLPLH YCFE L
Sbjct: 1 MISIASLEPPKQELSSS-SSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLF 59
Query: 3548 EFSDRPCLISGSDGRIYTYAETHLICQRTAVGLSRLGIKKGDVIMILLQNCPEFVFSFMG 3369
+ SD+PCLISGS G+ Y++AETHLI Q+TA GLS LGIKKGDVIMILLQNCPEFVFSFMG
Sbjct: 60 KLSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMG 119
Query: 3368 ASMLGAITTTANPFYTSAEIFKQVSASGAKLIITQSHYVDKLQHSNGISC----EDFKVV 3201
ASM+GA+ TTANPFYTS EIFKQ+++S KLIITQS YVDKL+ + EDF V+
Sbjct: 120 ASMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVI 179
Query: 3200 TINDPQENCIHFSEISNADELKI-EPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISS 3024
TI++P ENC+HF+ +S A E +I E ++ +P+D VALP+SSGTTGLPKGV+LTHKSLI+S
Sbjct: 180 TIDEPPENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITS 239
Query: 3023 VAQQVDGENPNLYLTADDIVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNL 2844
VAQQVDGENPNLYL DD+VLCVLPLFHIYSLNSVLLCSLR GA VLLMQKF+I LL L
Sbjct: 240 VAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLEL 299
Query: 2843 IQKHRVSXXXXXXXXXXXXAKDPIVDKFDLSSIRIVLSGAAPLGKELEDALRSRVPQAIF 2664
IQKH+VS AK+P+V +FDLSSIR+VLSGAAPLGKELEDALRSRVPQAI
Sbjct: 300 IQKHKVSVAAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAIL 359
Query: 2663 GQGYGMTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEIC 2484
GQGYGMTEAGPVLSMC GFAKQPFPTKSGSCGTVVRNAELK+IDPE+G SL NQPGEIC
Sbjct: 360 GQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 419
Query: 2483 IRGPQIMKGYLNDADATASTIDVEGWLHTGDIGSVDDDDEVFIVDRVKELIKFKGFQVPP 2304
IRGPQIMKGYLND +ATA+TIDVEGWLHTGDIG VDDDDE+FIVDRVKE+IKFKGFQVPP
Sbjct: 420 IRGPQIMKGYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPP 479
Query: 2303 AELEALLVSHPSIADAGVVPQMDAVAGEVPVAFIVPSNDFELTEEAVKEFIAKQVVFYKR 2124
AELEALL++HPSIADA VVPQ D VAGEVPVAF+V SN FEL+EE VKE+IAKQVVFYK+
Sbjct: 480 AELEALLLNHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELSEEDVKEYIAKQVVFYKK 539
Query: 2123 LHKVYFIHAIPKSPSGKILRKDLRAKLASSS 2031
LHKVYFIHAIPKSPSGKILRKDL+AKLA+ S
Sbjct: 540 LHKVYFIHAIPKSPSGKILRKDLKAKLATPS 570
>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
Length = 574
Score = 836 bits (2160), Expect = 0.0
Identities = 424/575 (73%), Positives = 483/575 (84%), Gaps = 9/575 (1%)
Frame = -3
Query: 3716 ITIASPETKPPQISPQVSTSETENTCPETFVFRSKLPDIPISNHLPLHAYCFERLSEFSD 3537
+ + SP KP Q+SP+ P +FRSKLPDIPISNHLPLH YCFE L SD
Sbjct: 9 VELESP--KPDQLSPET---------PADHIFRSKLPDIPISNHLPLHTYCFENLPSLSD 57
Query: 3536 RPCLISGSDGRIYTYAETHLICQRTAVGLSRLGIKKGDVIMILLQNCPEFVFSFMGASML 3357
+PCLISGS G+IY+++ETHLI Q+TA GLS+LGI+KGD IMILL NC EFVF+FMGASM+
Sbjct: 58 KPCLISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMI 117
Query: 3356 GAITTTANPFYTSAEIFKQVSASGAKLIITQSHYVDKLQHSNGISC---------EDFKV 3204
GA++TTANPFYTS EIFKQ A+ AKLIITQS YVDKL+ +N + EDFKV
Sbjct: 118 GAVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKV 177
Query: 3203 VTINDPQENCIHFSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISS 3024
+TI+DP ENC+ FS +S A+E +I V+I P+D VALP+SSGTTGLPKGV+LTHKSLI+S
Sbjct: 178 ITIDDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITS 237
Query: 3023 VAQQVDGENPNLYLTADDIVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNL 2844
VAQQVDGENPNLYLT +D+VLCVLPLFHIYSLNSVLLCSLR GA VLLMQKFEI TLL L
Sbjct: 238 VAQQVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLEL 297
Query: 2843 IQKHRVSXXXXXXXXXXXXAKDPIVDKFDLSSIRIVLSGAAPLGKELEDALRSRVPQAIF 2664
IQ+H+VS AK+P+V +FDLSSIR+VLSGAAPLGKELE+ALR RVPQA+
Sbjct: 298 IQRHKVSVAAVVPPLVLLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEEALRGRVPQAVL 357
Query: 2663 GQGYGMTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEIC 2484
GQGYGMTEAGPVLSMC GFAKQPFPTKSGSCGTVVRNAELK+IDPE+G SL NQPGEIC
Sbjct: 358 GQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 417
Query: 2483 IRGPQIMKGYLNDADATASTIDVEGWLHTGDIGSVDDDDEVFIVDRVKELIKFKGFQVPP 2304
IRG QIMKGYLND+ ATA+T DVEGWLHTGDIG VD+DDE+FIVDRVKE+IKFKGFQVPP
Sbjct: 418 IRGSQIMKGYLNDSVATAATTDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPP 477
Query: 2303 AELEALLVSHPSIADAGVVPQMDAVAGEVPVAFIVPSNDFELTEEAVKEFIAKQVVFYKR 2124
AELE+LL+SHPSIADA VVPQ D VAGEVPVAF+V SN FELTEEAVKEFIA+QVV+YKR
Sbjct: 478 AELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIARQVVYYKR 537
Query: 2123 LHKVYFIHAIPKSPSGKILRKDLRAKLASSS*EKH 2019
LHKV+F+HAIPKSPSGKILRKDLRAKLA+++ H
Sbjct: 538 LHKVHFVHAIPKSPSGKILRKDLRAKLAAAAPTTH 572
>gb|ADU15550.1| 4CL [Gossypium hirsutum]
Length = 574
Score = 836 bits (2159), Expect = 0.0
Identities = 424/567 (74%), Positives = 478/567 (84%), Gaps = 9/567 (1%)
Frame = -3
Query: 3692 KPPQISPQVSTSETENTCPETFVFRSKLPDIPISNHLPLHAYCFERLSEFSDRPCLISGS 3513
KP Q+SP+ P +FRSKLPDIPISNHLPLH YCFE L SD+PCLISGS
Sbjct: 15 KPDQLSPET---------PADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCLISGS 65
Query: 3512 DGRIYTYAETHLICQRTAVGLSRLGIKKGDVIMILLQNCPEFVFSFMGASMLGAITTTAN 3333
G+IY+++ETHLI Q+TA GLS+LGI+KGD IMILL NC EFVF+FMGASM+GA++TTAN
Sbjct: 66 SGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAVSTTAN 125
Query: 3332 PFYTSAEIFKQVSASGAKLIITQSHYVDKLQHSNGISC---------EDFKVVTINDPQE 3180
PFYTS EIFKQ A+ AKLIITQS YVDKL+ +N + EDFKV+TI+DP E
Sbjct: 126 PFYTSKEIFKQSKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVITIDDPPE 185
Query: 3179 NCIHFSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISSVAQQVDGE 3000
NC+ FS +S A+E +I V+I P+D VALP+SSGTTGLPKGV+LTHKSLI+SVAQQVDGE
Sbjct: 186 NCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGE 245
Query: 2999 NPNLYLTADDIVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNLIQKHRVSX 2820
NPNLYLT +D+VLCVLPLFHIYSLNSVLL SLR GA VLLMQKFEI TLL LIQ+H+VS
Sbjct: 246 NPNLYLTHEDVVLCVLPLFHIYSLNSVLLWSLRAGAAVLLMQKFEIGTLLELIQRHKVSV 305
Query: 2819 XXXXXXXXXXXAKDPIVDKFDLSSIRIVLSGAAPLGKELEDALRSRVPQAIFGQGYGMTE 2640
AK+P+V +FDLSSIR+VLSGAAPLGKELEDALR RVPQA+ GQGYGMTE
Sbjct: 306 AAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLGQGYGMTE 365
Query: 2639 AGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEICIRGPQIMK 2460
AGPVLSMC GFAKQPFPTKSGSCGTVVRNAELK+IDPE+G SL NQPGEICIRG QIMK
Sbjct: 366 AGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGSQIMK 425
Query: 2459 GYLNDADATASTIDVEGWLHTGDIGSVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLV 2280
GYLND ATA+TIDVEGWLHTGDIG VD+DDE+FIVDRVKE+IKFKGFQVPPAELE+LL+
Sbjct: 426 GYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAELESLLI 485
Query: 2279 SHPSIADAGVVPQMDAVAGEVPVAFIVPSNDFELTEEAVKEFIAKQVVFYKRLHKVYFIH 2100
SHPSIADA VVPQ D VAGEVPVAF+V SN FELTEEAVKEFIAKQVV+YKRLHKV+F+H
Sbjct: 486 SHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVYYKRLHKVHFVH 545
Query: 2099 AIPKSPSGKILRKDLRAKLASSS*EKH 2019
AIPKSPSGKILRKDLRAKLA+++ H
Sbjct: 546 AIPKSPSGKILRKDLRAKLAAAAPNTH 572