BLASTX nr result
ID: Coptis23_contig00012609
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00012609
(3088 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM65226.1| putative translation initiation factor eIF-2, gam... 359 e-176
ref|XP_003531501.1| PREDICTED: eukaryotic translation initiation... 370 e-150
ref|NP_001241486.1| uncharacterized protein LOC100784099 [Glycin... 370 e-150
ref|XP_002273799.1| PREDICTED: eukaryotic translation initiation... 370 e-150
ref|XP_002271240.1| PREDICTED: eukaryotic translation initiation... 370 e-150
>gb|AAM65226.1| putative translation initiation factor eIF-2, gamma subunit
[Arabidopsis thaliana]
Length = 465
Score = 359 bits (922), Expect(3) = e-176
Identities = 174/219 (79%), Positives = 199/219 (90%)
Frame = -2
Query: 1716 MSRKDLMEQDVSKLGPLSPKVISRQATINIGTIGHVAHGKSTVVKAISGIQTVRFKNELE 1537
++ +DL + DV+ L PLSP+VISRQATINIGTIGHVAHGKSTVVKAISG+QTVRFKNELE
Sbjct: 7 LAEQDLKKLDVTVLHPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELE 66
Query: 1536 RNITIKLGYANAKIYKCENERCPRPFCYNSYESGKEGSPICDVPGCANARMKLLQHLSFV 1357
RNITIKLGYANAKIYKCE+E+CPRP CY +Y SGKE +P CDVPG N++MKLL+H+SFV
Sbjct: 67 RNITIKLGYANAKIYKCEDEKCPRPMCYKAYGSGKEDTPNCDVPGFENSKMKLLRHVSFV 126
Query: 1356 DCPGHDILMATLLNGGAIMDGALLLIAANESCPQPQTSEHLAALEIMQLNHIIILQNKVD 1177
DCPGHDILMAT+LNG AIMDGALLLIAANE+CPQPQTSEHLAA+EIMQL HIIILQNK+D
Sbjct: 127 DCPGHDILMATMLNGAAIMDGALLLIAANETCPQPQTSEHLAAVEIMQLKHIIILQNKID 186
Query: 1176 LVQEDVAINQHEAIQTLIKHSVADGAPIILISGQLKYNI 1060
L+QE+VAINQHEAIQ I ++VAD API+ +S QLKYNI
Sbjct: 187 LIQENVAINQHEAIQKFIMNTVADAAPIVPVSAQLKYNI 225
Score = 185 bits (469), Expect(3) = e-176
Identities = 92/115 (80%), Positives = 102/115 (88%)
Frame = -1
Query: 1060 IDVVCEYIVKKIPVPVRDFVSPPNMIIIRSFDVNKPGSGVDDLKGGVAGGSILRGILKVN 881
IDVVCEYIVKKIP+P R+FVSPPNMI+IRSFDVNKPG VD++KGGVAGGSILRG+L+VN
Sbjct: 225 IDVVCEYIVKKIPIPERNFVSPPNMIVIRSFDVNKPGYEVDEIKGGVAGGSILRGVLRVN 284
Query: 880 QTIEVRLGIVNKDESGKIISCTLIYSRIVSLFAEQNELQFAVPGGLIGVGTTMDP 716
Q IE+R GIV KDE G CT IYSRI+SL+AEQNELQFAVP GLIGVGTTMDP
Sbjct: 285 QLIEIRPGIVTKDERGN-SKCTPIYSRIISLYAEQNELQFAVPEGLIGVGTTMDP 338
Score = 126 bits (316), Expect(3) = e-176
Identities = 69/140 (49%), Positives = 84/140 (60%)
Frame = -2
Query: 738 GLALLWTPMLTRADRLVGQVLGEVGSLPEIYIEXXXXXXXXXXXXXXXXXXTESQGNVSK 559
G+ P LTRADRLVGQVLGE+GSLP++++E +E QG VSK
Sbjct: 331 GVGTTMDPTLTRADRLVGQVLGEIGSLPDVFVELEVNFFLLRRLLGVRTKGSEKQGKVSK 390
Query: 558 LTKNEDLMLNIGSMSTGAXXXXXXXXXXXXVCRLLLTSPVCTRVDVKVVLSRKIEHHWRL 379
LTK E LMLNIGSMSTGA +L LT+PVCT KV LSR++E HWRL
Sbjct: 391 LTKGEILMLNIGSMSTGAKVVGVKVDL----AKLQLTAPVCTSKGEKVALSRRVEKHWRL 446
Query: 378 IGWGKVTEGVAVDVVSPLPF 319
IGWG++ G ++ V P PF
Sbjct: 447 IGWGQIQAGTTIE-VPPSPF 465
>ref|XP_003531501.1| PREDICTED: eukaryotic translation initiation factor 2 subunit 3
[Glycine max]
Length = 466
Score = 370 bits (949), Expect(2) = e-150
Identities = 184/224 (82%), Positives = 203/224 (90%), Gaps = 5/224 (2%)
Frame = -2
Query: 1716 MSRKDLMEQDVSKLG-----PLSPKVISRQATINIGTIGHVAHGKSTVVKAISGIQTVRF 1552
MSRK LMEQD+SKL PLSP+VISRQATINIGTIGHVAHGKSTVVKAISG+QTVRF
Sbjct: 1 MSRKGLMEQDLSKLDVTKLHPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRF 60
Query: 1551 KNELERNITIKLGYANAKIYKCENERCPRPFCYNSYESGKEGSPICDVPGCANARMKLLQ 1372
KNELERNITIKLGYANAKIYKCE+ERCPRP CY +Y SGKE SP+CDVPG N++MKLL+
Sbjct: 61 KNELERNITIKLGYANAKIYKCEDERCPRPMCYKAYGSGKEDSPMCDVPGFENSKMKLLR 120
Query: 1371 HLSFVDCPGHDILMATLLNGGAIMDGALLLIAANESCPQPQTSEHLAALEIMQLNHIIIL 1192
H+SFVDCPGHDILMAT+LNG AIMDGALLLIAANESCPQPQTSEHLAA+EIM+L HIIIL
Sbjct: 121 HVSFVDCPGHDILMATMLNGAAIMDGALLLIAANESCPQPQTSEHLAAVEIMRLQHIIIL 180
Query: 1191 QNKVDLVQEDVAINQHEAIQTLIKHSVADGAPIILISGQLKYNI 1060
QNKVDL+QE+VAINQHEAI I+ +VADGAP++ IS QLKYNI
Sbjct: 181 QNKVDLIQENVAINQHEAISKFIQGTVADGAPVVPISAQLKYNI 224
Score = 192 bits (488), Expect(2) = e-150
Identities = 98/115 (85%), Positives = 105/115 (91%)
Frame = -1
Query: 1060 IDVVCEYIVKKIPVPVRDFVSPPNMIIIRSFDVNKPGSGVDDLKGGVAGGSILRGILKVN 881
IDVVCEYIVKKIP+P R+FVSPPNMI+IRSFDVNKPG VD++KGGVAGGSILRG+LKVN
Sbjct: 224 IDVVCEYIVKKIPIPERNFVSPPNMIVIRSFDVNKPGYEVDEIKGGVAGGSILRGVLKVN 283
Query: 880 QTIEVRLGIVNKDESGKIISCTLIYSRIVSLFAEQNELQFAVPGGLIGVGTTMDP 716
Q IEVR GIV KDESG I CT IYSRIVSL+AEQNELQFAVPGGLIGVGTTMDP
Sbjct: 284 QFIEVRPGIVVKDESGN-IRCTPIYSRIVSLYAEQNELQFAVPGGLIGVGTTMDP 337
Score = 126 bits (317), Expect = 3e-26
Identities = 69/138 (50%), Positives = 82/138 (59%)
Frame = -2
Query: 750 GDSSGLALLWTPMLTRADRLVGQVLGEVGSLPEIYIEXXXXXXXXXXXXXXXXXXTESQG 571
G G+ P LTRADRLVGQVLGEVGSLPE+++E +E QG
Sbjct: 326 GGLIGVGTTMDPTLTRADRLVGQVLGEVGSLPEVFVELEVNFFLLRRLLGVRTKGSERQG 385
Query: 570 NVSKLTKNEDLMLNIGSMSTGAXXXXXXXXXXXXVCRLLLTSPVCTRVDVKVVLSRKIEH 391
VSKL K E LMLNIGSMSTGA +L LTSPVCT K+ LSR++E
Sbjct: 386 KVSKLAKGEMLMLNIGSMSTGARVVAVKNDL----AKLQLTSPVCTSKGEKIALSRRVEK 441
Query: 390 HWRLIGWGKVTEGVAVDV 337
HWRLIGWG++ G+ +DV
Sbjct: 442 HWRLIGWGQIQAGITLDV 459
>ref|NP_001241486.1| uncharacterized protein LOC100784099 [Glycine max]
gi|255637109|gb|ACU18886.1| unknown [Glycine max]
Length = 466
Score = 370 bits (949), Expect(2) = e-150
Identities = 184/224 (82%), Positives = 203/224 (90%), Gaps = 5/224 (2%)
Frame = -2
Query: 1716 MSRKDLMEQDVSKLG-----PLSPKVISRQATINIGTIGHVAHGKSTVVKAISGIQTVRF 1552
MSRK LMEQD+SKL PLSP+VISRQATINIGTIGHVAHGKSTVVKAISG+QTVRF
Sbjct: 1 MSRKGLMEQDLSKLDVTKLHPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRF 60
Query: 1551 KNELERNITIKLGYANAKIYKCENERCPRPFCYNSYESGKEGSPICDVPGCANARMKLLQ 1372
KNELERNITIKLGYANAKIYKCE+ERCPRP CY +Y SGKE SP+CDVPG N++MKLL+
Sbjct: 61 KNELERNITIKLGYANAKIYKCEDERCPRPMCYKAYGSGKEDSPMCDVPGFENSKMKLLR 120
Query: 1371 HLSFVDCPGHDILMATLLNGGAIMDGALLLIAANESCPQPQTSEHLAALEIMQLNHIIIL 1192
H+SFVDCPGHDILMAT+LNG AIMDGALLLIAANESCPQPQTSEHLAA+EIM+L HIIIL
Sbjct: 121 HVSFVDCPGHDILMATMLNGAAIMDGALLLIAANESCPQPQTSEHLAAVEIMRLQHIIIL 180
Query: 1191 QNKVDLVQEDVAINQHEAIQTLIKHSVADGAPIILISGQLKYNI 1060
QNKVDL+QE+VAINQHEAI I+ +VADGAP++ IS QLKYNI
Sbjct: 181 QNKVDLIQENVAINQHEAISKFIQGTVADGAPVVPISAQLKYNI 224
Score = 192 bits (488), Expect(2) = e-150
Identities = 98/115 (85%), Positives = 105/115 (91%)
Frame = -1
Query: 1060 IDVVCEYIVKKIPVPVRDFVSPPNMIIIRSFDVNKPGSGVDDLKGGVAGGSILRGILKVN 881
IDVVCEYIVKKIP+P R+FVSPPNMI+IRSFDVNKPG VD++KGGVAGGSILRG+LKVN
Sbjct: 224 IDVVCEYIVKKIPIPERNFVSPPNMIVIRSFDVNKPGYEVDEIKGGVAGGSILRGVLKVN 283
Query: 880 QTIEVRLGIVNKDESGKIISCTLIYSRIVSLFAEQNELQFAVPGGLIGVGTTMDP 716
Q IEVR GIV KDESG I CT IYSRIVSL+AEQNELQFAVPGGLIGVGTTMDP
Sbjct: 284 QFIEVRPGIVVKDESGN-IRCTPIYSRIVSLYAEQNELQFAVPGGLIGVGTTMDP 337
Score = 126 bits (317), Expect = 3e-26
Identities = 69/138 (50%), Positives = 82/138 (59%)
Frame = -2
Query: 750 GDSSGLALLWTPMLTRADRLVGQVLGEVGSLPEIYIEXXXXXXXXXXXXXXXXXXTESQG 571
G G+ P LTRADRLVGQVLGEVGSLPE+++E +E QG
Sbjct: 326 GGLIGVGTTMDPTLTRADRLVGQVLGEVGSLPEVFVELEVNFFLLRRLLGVRTKGSERQG 385
Query: 570 NVSKLTKNEDLMLNIGSMSTGAXXXXXXXXXXXXVCRLLLTSPVCTRVDVKVVLSRKIEH 391
VSKL K E LMLNIGSMSTGA +L LTSPVCT K+ LSR++E
Sbjct: 386 KVSKLAKGEMLMLNIGSMSTGARVVAVKNDL----AKLQLTSPVCTSKGEKIALSRRVEK 441
Query: 390 HWRLIGWGKVTEGVAVDV 337
HWRLIGWG++ G+ +DV
Sbjct: 442 HWRLIGWGQIQAGITLDV 459
>ref|XP_002273799.1| PREDICTED: eukaryotic translation initiation factor 2 subunit 3
[Vitis vinifera] gi|147852071|emb|CAN80178.1|
hypothetical protein VITISV_010162 [Vitis vinifera]
Length = 464
Score = 370 bits (951), Expect(2) = e-150
Identities = 184/224 (82%), Positives = 203/224 (90%), Gaps = 5/224 (2%)
Frame = -2
Query: 1716 MSRKDLMEQDVSKLG-----PLSPKVISRQATINIGTIGHVAHGKSTVVKAISGIQTVRF 1552
MSR+ LMEQD+SKL PLSP+VISRQATINIGTIGHVAHGKSTVVKAISG+QTVRF
Sbjct: 1 MSRRGLMEQDLSKLDVTTLHPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRF 60
Query: 1551 KNELERNITIKLGYANAKIYKCENERCPRPFCYNSYESGKEGSPICDVPGCANARMKLLQ 1372
KNELERNITIKLGYANAKIYKCE+ERCPRP CY +Y SGKE SP+CDVPG N RMKLL+
Sbjct: 61 KNELERNITIKLGYANAKIYKCEDERCPRPMCYKAYGSGKEDSPLCDVPGFENCRMKLLR 120
Query: 1371 HLSFVDCPGHDILMATLLNGGAIMDGALLLIAANESCPQPQTSEHLAALEIMQLNHIIIL 1192
H+SFVDCPGHDILMAT+LNG AIMDGALLLIAANESCPQPQT+EHLAA+EIM+L HIIIL
Sbjct: 121 HVSFVDCPGHDILMATMLNGAAIMDGALLLIAANESCPQPQTAEHLAAVEIMRLQHIIIL 180
Query: 1191 QNKVDLVQEDVAINQHEAIQTLIKHSVADGAPIILISGQLKYNI 1060
QNKVDL+QE+VAINQHEAIQ I+ +VADGAP++ IS QLKYNI
Sbjct: 181 QNKVDLIQENVAINQHEAIQKFIQGTVADGAPVVPISAQLKYNI 224
Score = 191 bits (484), Expect(2) = e-150
Identities = 96/115 (83%), Positives = 104/115 (90%)
Frame = -1
Query: 1060 IDVVCEYIVKKIPVPVRDFVSPPNMIIIRSFDVNKPGSGVDDLKGGVAGGSILRGILKVN 881
IDVVCEYIVKKIP+P RDF SPPNMI+IRSFDVNKPG VD+++GGVAGGSILRG+LKVN
Sbjct: 224 IDVVCEYIVKKIPIPERDFTSPPNMIVIRSFDVNKPGFEVDEIRGGVAGGSILRGVLKVN 283
Query: 880 QTIEVRLGIVNKDESGKIISCTLIYSRIVSLFAEQNELQFAVPGGLIGVGTTMDP 716
Q IEVR GIV KDE+G I CT IYSRIVSL+AEQNELQFAVPGGLIGVGTTMDP
Sbjct: 284 QFIEVRPGIVVKDENGN-IKCTPIYSRIVSLYAEQNELQFAVPGGLIGVGTTMDP 337
Score = 126 bits (316), Expect = 4e-26
Identities = 70/138 (50%), Positives = 82/138 (59%)
Frame = -2
Query: 750 GDSSGLALLWTPMLTRADRLVGQVLGEVGSLPEIYIEXXXXXXXXXXXXXXXXXXTESQG 571
G G+ P LTRADRLVGQVLGEVGSLP++++E TE QG
Sbjct: 326 GGLIGVGTTMDPTLTRADRLVGQVLGEVGSLPDVFVELEVNFFLLRRLIGVRTKGTEKQG 385
Query: 570 NVSKLTKNEDLMLNIGSMSTGAXXXXXXXXXXXXVCRLLLTSPVCTRVDVKVVLSRKIEH 391
VSKLTK E LMLNIGSMSTGA +L LTSPVCT K+ LSR++E
Sbjct: 386 KVSKLTKGEILMLNIGSMSTGARVLAVRNDL----AKLQLTSPVCTSRGEKIALSRRVEK 441
Query: 390 HWRLIGWGKVTEGVAVDV 337
HWRLIGWG++ G +DV
Sbjct: 442 HWRLIGWGQIQAGTTLDV 459
>ref|XP_002271240.1| PREDICTED: eukaryotic translation initiation factor 2 subunit 3
[Vitis vinifera] gi|296090387|emb|CBI40206.3| unnamed
protein product [Vitis vinifera]
Length = 464
Score = 370 bits (950), Expect(2) = e-150
Identities = 183/224 (81%), Positives = 203/224 (90%), Gaps = 5/224 (2%)
Frame = -2
Query: 1716 MSRKDLMEQDVSKLG-----PLSPKVISRQATINIGTIGHVAHGKSTVVKAISGIQTVRF 1552
MSR+ LMEQD+SKL PLSP+VISRQATINIGT+GHVAHGKSTVVKAISG+QTVRF
Sbjct: 1 MSRRGLMEQDLSKLDVTTLHPLSPEVISRQATINIGTVGHVAHGKSTVVKAISGVQTVRF 60
Query: 1551 KNELERNITIKLGYANAKIYKCENERCPRPFCYNSYESGKEGSPICDVPGCANARMKLLQ 1372
KNELERNITIKLGYANAKIYKCE+ERCPRP CY +Y SGKE SP+CDVPG N RMKLL+
Sbjct: 61 KNELERNITIKLGYANAKIYKCEDERCPRPMCYKAYGSGKEDSPLCDVPGFENCRMKLLR 120
Query: 1371 HLSFVDCPGHDILMATLLNGGAIMDGALLLIAANESCPQPQTSEHLAALEIMQLNHIIIL 1192
H+SFVDCPGHDILMAT+LNG AIMDGALLLIAANESCPQPQT+EHLAA+EIM+L HIIIL
Sbjct: 121 HVSFVDCPGHDILMATMLNGAAIMDGALLLIAANESCPQPQTAEHLAAVEIMRLQHIIIL 180
Query: 1191 QNKVDLVQEDVAINQHEAIQTLIKHSVADGAPIILISGQLKYNI 1060
QNKVDL+QE+VAINQHEAIQ I+ +VADGAP++ IS QLKYNI
Sbjct: 181 QNKVDLIQENVAINQHEAIQKFIQGTVADGAPVVPISAQLKYNI 224
Score = 191 bits (484), Expect(2) = e-150
Identities = 96/115 (83%), Positives = 104/115 (90%)
Frame = -1
Query: 1060 IDVVCEYIVKKIPVPVRDFVSPPNMIIIRSFDVNKPGSGVDDLKGGVAGGSILRGILKVN 881
IDVVCEYIVKKIP+P RDF SPPNMI+IRSFDVNKPG VD+++GGVAGGSILRG+LKVN
Sbjct: 224 IDVVCEYIVKKIPIPERDFTSPPNMIVIRSFDVNKPGFEVDEIRGGVAGGSILRGVLKVN 283
Query: 880 QTIEVRLGIVNKDESGKIISCTLIYSRIVSLFAEQNELQFAVPGGLIGVGTTMDP 716
Q IEVR GIV KDE+G I CT IYSRIVSL+AEQNELQFAVPGGLIGVGTTMDP
Sbjct: 284 QFIEVRPGIVVKDENGN-IKCTPIYSRIVSLYAEQNELQFAVPGGLIGVGTTMDP 337
Score = 125 bits (315), Expect = 5e-26
Identities = 69/138 (50%), Positives = 82/138 (59%)
Frame = -2
Query: 750 GDSSGLALLWTPMLTRADRLVGQVLGEVGSLPEIYIEXXXXXXXXXXXXXXXXXXTESQG 571
G G+ P LTRADRLVGQVLGEVGSLP++++E TE QG
Sbjct: 326 GGLIGVGTTMDPTLTRADRLVGQVLGEVGSLPDVFVELEVNFFLLRRLIGVRAKGTEKQG 385
Query: 570 NVSKLTKNEDLMLNIGSMSTGAXXXXXXXXXXXXVCRLLLTSPVCTRVDVKVVLSRKIEH 391
VSKLTK E LMLNIGSMSTGA +L LTSPVCT K+ LSR++E
Sbjct: 386 KVSKLTKGEILMLNIGSMSTGARVLAVRNDL----AKLQLTSPVCTSRGEKIALSRRVEK 441
Query: 390 HWRLIGWGKVTEGVAVDV 337
HWRLIGWG++ G +D+
Sbjct: 442 HWRLIGWGQIQAGTTLDI 459