BLASTX nr result
ID: Coptis23_contig00012539
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00012539
(2036 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI37301.3| unnamed protein product [Vitis vinifera] 234 e-110
ref|XP_002265267.1| PREDICTED: uncharacterized protein LOC100244... 226 e-108
ref|XP_002521109.1| conserved hypothetical protein [Ricinus comm... 238 5e-70
ref|XP_002301777.1| predicted protein [Populus trichocarpa] gi|2... 223 3e-67
ref|NP_568703.1| uncharacterized protein [Arabidopsis thaliana] ... 194 2e-56
>emb|CBI37301.3| unnamed protein product [Vitis vinifera]
Length = 511
Score = 234 bits (598), Expect(3) = e-110
Identities = 128/238 (53%), Positives = 169/238 (71%)
Frame = -1
Query: 1487 TFFFCAGRSDQQVNVEYAWAEVKSKLLGALDAAKQDGGLESRVVESGDYSTKCPLSLYAV 1308
TF A ++ QVN++ AW VK KLL +L+A + G LE+ VVES + K PLSL+A+
Sbjct: 136 TFSGSALKNGDQVNLKCAWDVVKGKLLDSLNAIEHIGSLENGVVES-EQRAKRPLSLHAI 194
Query: 1307 ADGPRLRLLWTALQQLEKEVNNISGSYQVVDRGDWMGTSSEVLQISSQSVFVTWLEEELN 1128
ADGPRLRLLW + QQLEKEV NIS + + V DW+ SE++Q Q + + WLE+E
Sbjct: 195 ADGPRLRLLWASFQQLEKEVENISENSETVTMDDWLILFSEIIQKLCQPICMAWLEKECT 254
Query: 1127 LECKKHDKNILLMMLQKLKQDDNITKNIKRSGKDELYADLLYFLRFRFLSTGCRYDNKLL 948
E +K DK + +M +KL+ D +I + I+ SGK++L+A+LL FLRF L GC YD+ L
Sbjct: 255 -ENRKADKALHTLMFEKLEGDTSILQKIRNSGKEDLFAELLCFLRFGSLREGCCYDHSLF 313
Query: 947 AHHGVDILEDLVITLADGITTNYLELISVDSNMSSDMNRMGLNLCSLSTRALQKLRNE 774
HGV ILEDLVITLADGI++ YLELISVD N+S++MN +G LC+LSTRALQ+LRNE
Sbjct: 314 TLHGVVILEDLVITLADGISSVYLELISVDGNVSNEMNSLGWRLCTLSTRALQRLRNE 371
Score = 135 bits (339), Expect(3) = e-110
Identities = 67/114 (58%), Positives = 81/114 (71%)
Frame = -2
Query: 718 SFQIQLTKEADKSRQDXXXXXXXXXXXXXXPVVKPLQFMVISPLSMPVKRTRELRALNGW 539
+ Q QL +E KS+ + + P Q +VIS SMPVKRT+ELRAL GW
Sbjct: 397 TLQSQLIEEPSKSQAENFSWWKKLTVRQSGSLSTPSQCVVISHFSMPVKRTKELRALTGW 456
Query: 538 RYYFSLFLEFSDITMPLVRVVFSKICSAISFFLVCLVGRSLGLIYSGIRQSLGW 377
RYYFSLFLE SDITMPL+R +F+K+ A+SFFLVCL+GRSLGLIY+GIRQSL W
Sbjct: 457 RYYFSLFLELSDITMPLIRAIFTKVSDAVSFFLVCLIGRSLGLIYTGIRQSLRW 510
Score = 80.1 bits (196), Expect(3) = e-110
Identities = 49/131 (37%), Positives = 72/131 (54%), Gaps = 1/131 (0%)
Frame = -2
Query: 1903 MAEQATIPSCAKLQLPRSFYKFSHFVPRTVCLRIVQRGFKKHCYGSSNVL-VSAPLVCDF 1727
MAE A +PS KL + R K +PR ++VQR K YGS V +S+ +C
Sbjct: 1 MAEYAAVPSYMKLDMLRISIKERGLIPRDSYFKVVQR---KCSYGSYKVARLSSWQICVS 57
Query: 1726 NAHISTILERSANLKDSSMRCCCFGTLISPDNATVPNWVPVLDQALMMFSIVLAYIAGVV 1547
A + + L+ + K+ S+RC C D AT +WVPV+DQ L+M SI L Y+AGV+
Sbjct: 58 TAPVVSNLKITHCSKEGSLRCSCLA-----DGATTSDWVPVVDQVLLMASIFLTYMAGVI 112
Query: 1546 PPKNAYLKPEK 1514
P + +Y ++
Sbjct: 113 PTEKSYFSSQR 123
>ref|XP_002265267.1| PREDICTED: uncharacterized protein LOC100244728 [Vitis vinifera]
Length = 508
Score = 226 bits (577), Expect(3) = e-108
Identities = 125/238 (52%), Positives = 167/238 (70%)
Frame = -1
Query: 1487 TFFFCAGRSDQQVNVEYAWAEVKSKLLGALDAAKQDGGLESRVVESGDYSTKCPLSLYAV 1308
TF A ++ QVN++ AW VK KLL +L+A + G LE+ VVES + K PLSL+A+
Sbjct: 136 TFSGSALKNGDQVNLKCAWDVVKGKLLDSLNAIEHIGSLENGVVES-EQRAKRPLSLHAI 194
Query: 1307 ADGPRLRLLWTALQQLEKEVNNISGSYQVVDRGDWMGTSSEVLQISSQSVFVTWLEEELN 1128
ADGPRLRLLW + QQLEKE ++S + V DW+ SE++Q Q + + WLE+E
Sbjct: 195 ADGPRLRLLWASFQQLEKEAGSVS---ETVTMDDWLILFSEIIQKLCQPICMAWLEKECT 251
Query: 1127 LECKKHDKNILLMMLQKLKQDDNITKNIKRSGKDELYADLLYFLRFRFLSTGCRYDNKLL 948
E +K DK + +M +KL+ D +I + I+ SGK++L+A+LL FLRF L GC YD+ L
Sbjct: 252 -ENRKADKALHTLMFEKLEGDTSILQKIRNSGKEDLFAELLCFLRFGSLREGCCYDHSLF 310
Query: 947 AHHGVDILEDLVITLADGITTNYLELISVDSNMSSDMNRMGLNLCSLSTRALQKLRNE 774
HGV ILEDLVITLADGI++ YLELISVD N+S++MN +G LC+LSTRALQ+LRNE
Sbjct: 311 TLHGVVILEDLVITLADGISSVYLELISVDGNVSNEMNSLGWRLCTLSTRALQRLRNE 368
Score = 135 bits (339), Expect(3) = e-108
Identities = 67/114 (58%), Positives = 81/114 (71%)
Frame = -2
Query: 718 SFQIQLTKEADKSRQDXXXXXXXXXXXXXXPVVKPLQFMVISPLSMPVKRTRELRALNGW 539
+ Q QL +E KS+ + + P Q +VIS SMPVKRT+ELRAL GW
Sbjct: 394 TLQSQLIEEPSKSQAENFSWWKKLTVRQSGSLSTPSQCVVISHFSMPVKRTKELRALTGW 453
Query: 538 RYYFSLFLEFSDITMPLVRVVFSKICSAISFFLVCLVGRSLGLIYSGIRQSLGW 377
RYYFSLFLE SDITMPL+R +F+K+ A+SFFLVCL+GRSLGLIY+GIRQSL W
Sbjct: 454 RYYFSLFLELSDITMPLIRAIFTKVSDAVSFFLVCLIGRSLGLIYTGIRQSLRW 507
Score = 80.1 bits (196), Expect(3) = e-108
Identities = 49/131 (37%), Positives = 72/131 (54%), Gaps = 1/131 (0%)
Frame = -2
Query: 1903 MAEQATIPSCAKLQLPRSFYKFSHFVPRTVCLRIVQRGFKKHCYGSSNVL-VSAPLVCDF 1727
MAE A +PS KL + R K +PR ++VQR K YGS V +S+ +C
Sbjct: 1 MAEYAAVPSYMKLDMLRISIKERGLIPRDSYFKVVQR---KCSYGSYKVARLSSWQICVS 57
Query: 1726 NAHISTILERSANLKDSSMRCCCFGTLISPDNATVPNWVPVLDQALMMFSIVLAYIAGVV 1547
A + + L+ + K+ S+RC C D AT +WVPV+DQ L+M SI L Y+AGV+
Sbjct: 58 TAPVVSNLKITHCSKEGSLRCSCLA-----DGATTSDWVPVVDQVLLMASIFLTYMAGVI 112
Query: 1546 PPKNAYLKPEK 1514
P + +Y ++
Sbjct: 113 PTEKSYFSSQR 123
>ref|XP_002521109.1| conserved hypothetical protein [Ricinus communis]
gi|223539678|gb|EEF41260.1| conserved hypothetical
protein [Ricinus communis]
Length = 517
Score = 238 bits (607), Expect(2) = 5e-70
Identities = 119/233 (51%), Positives = 163/233 (69%), Gaps = 3/233 (1%)
Frame = -1
Query: 1463 SDQQVNVEYAWAEVKSKLLGALDAAKQDGGLESRVVESGDYSTKCPLSLYAVADGPRLRL 1284
+D VN +YAW VK KLL +LD + L SR +E + K PLSLYAV++GP+LRL
Sbjct: 146 NDNPVNSKYAWDAVKEKLLDSLDTLEHKNNLRSRFLEVEQHQAKRPLSLYAVSEGPKLRL 205
Query: 1283 LWTALQQLEKE---VNNISGSYQVVDRGDWMGTSSEVLQISSQSVFVTWLEEELNLECKK 1113
LW + + LE E VNN+ G Y+ + DW+ ++++ S V ++WL EEL LE KK
Sbjct: 206 LWASFKHLEDEAGTVNNVLGDYEALTLDDWLNKFPDIIRKSCNHVSISWLLEELRLENKK 265
Query: 1112 HDKNILLMMLQKLKQDDNITKNIKRSGKDELYADLLYFLRFRFLSTGCRYDNKLLAHHGV 933
D+ I+ +M+QKLK D+ I +NI++SGK++LYA+LLYFLRF L C YD L + HG
Sbjct: 266 QDEAIVSLMIQKLKGDETILQNIRKSGKEDLYAELLYFLRFGSLRKNCCYDQSLFSAHGD 325
Query: 932 DILEDLVITLADGITTNYLELISVDSNMSSDMNRMGLNLCSLSTRALQKLRNE 774
ILEDLVI LADG+ + YLELISVD N+S++M+ +G+ +C+LSTRALQ+LRNE
Sbjct: 326 SILEDLVIALADGVASVYLELISVDGNLSNEMSDLGMIMCNLSTRALQRLRNE 378
Score = 55.1 bits (131), Expect(2) = 5e-70
Identities = 39/125 (31%), Positives = 60/125 (48%), Gaps = 1/125 (0%)
Frame = -2
Query: 1903 MAEQATIPSCAKLQLPRSFYKFSHFVPRTVCLRIVQRGFKKHCYGSSNVLVSAPL-VCDF 1727
MAE SC KL + RS K ++ Q +K+ GS V P C
Sbjct: 1 MAEYTASTSCVKLHMWRSCVKEKGIS----LIQNSQTPKRKYSQGSCKVFHFPPRKTCST 56
Query: 1726 NAHISTILERSANLKDSSMRCCCFGTLISPDNATVPNWVPVLDQALMMFSIVLAYIAGVV 1547
+ ++ S ++S +R C G+L+ PD T + V + DQ L++ SI L Y+AGV+
Sbjct: 57 GL---SYMKASPYTRESYLRYRCLGSLVDPDGVTASDLVAISDQLLLVASIALTYMAGVI 113
Query: 1546 PPKNA 1532
P K++
Sbjct: 114 PNKSS 118
Score = 125 bits (313), Expect = 6e-26
Identities = 60/80 (75%), Positives = 68/80 (85%)
Frame = -2
Query: 613 LQFMVISPLSMPVKRTRELRALNGWRYYFSLFLEFSDITMPLVRVVFSKICSAISFFLVC 434
L ++VIS SMPVKRTRELRAL GWRYYFSL+LE DITMPL+R V K+ SAISFFLV
Sbjct: 438 LSYVVISQFSMPVKRTRELRALTGWRYYFSLYLELFDITMPLIRAVIEKVSSAISFFLVT 497
Query: 433 LVGRSLGLIYSGIRQSLGWR 374
L+GRSLGLIY+GIRQSL W+
Sbjct: 498 LIGRSLGLIYTGIRQSLRWK 517
>ref|XP_002301777.1| predicted protein [Populus trichocarpa] gi|222843503|gb|EEE81050.1|
predicted protein [Populus trichocarpa]
Length = 472
Score = 223 bits (567), Expect(2) = 3e-67
Identities = 112/231 (48%), Positives = 160/231 (69%)
Frame = -1
Query: 1466 RSDQQVNVEYAWAEVKSKLLGALDAAKQDGGLESRVVESGDYSTKCPLSLYAVADGPRLR 1287
+ D VN+++AW VK KLL +LDA ++ L ++ ++ K PLSL+A++D P+LR
Sbjct: 106 KKDDHVNLKHAWDAVKEKLLDSLDAIERRSNLGNKFLQFEQQHAKRPLSLFAISDSPKLR 165
Query: 1286 LLWTALQQLEKEVNNISGSYQVVDRGDWMGTSSEVLQISSQSVFVTWLEEELNLECKKHD 1107
LLWT+ Q LE+E + + V DW+ E++Q S V + WL EEL LE KK D
Sbjct: 166 LLWTSFQWLEEEAGTATTA---VSMDDWLAIFPEIIQKSCHPVCMAWLVEELQLENKKSD 222
Query: 1106 KNILLMMLQKLKQDDNITKNIKRSGKDELYADLLYFLRFRFLSTGCRYDNKLLAHHGVDI 927
K ++ +M++KLK DD + +NI++SGK++LYA+LLYFLRF L C YD +L HG I
Sbjct: 223 KELVSLMIEKLKGDDIVLQNIRKSGKEDLYAELLYFLRFGSLRKSCCYDCRLFILHGDSI 282
Query: 926 LEDLVITLADGITTNYLELISVDSNMSSDMNRMGLNLCSLSTRALQKLRNE 774
LEDLVITLADGI + YLE+ISVD ++S++MN + + +C++STRALQ+LRNE
Sbjct: 283 LEDLVITLADGIASVYLEIISVDGSLSNEMNSLDMFMCNMSTRALQRLRNE 333
Score = 61.2 bits (147), Expect(2) = 3e-67
Identities = 31/84 (36%), Positives = 49/84 (58%)
Frame = -2
Query: 1783 KHCYGSSNVLVSAPLVCDFNAHISTILERSANLKDSSMRCCCFGTLISPDNATVPNWVPV 1604
K+ + + V+ + L F+ +S + S + S++CCC G+L+ P+ A V WV V
Sbjct: 1 KYSHRNCKVVQVSTLKTCFDGLLS--FKASPVTRKDSVKCCCLGSLVDPNGAMVSGWVSV 58
Query: 1603 LDQALMMFSIVLAYIAGVVPPKNA 1532
DQ L+M SI L Y+AGV+P N+
Sbjct: 59 SDQLLLMASIFLTYMAGVIPQHNS 82
Score = 120 bits (300), Expect = 2e-24
Identities = 58/78 (74%), Positives = 65/78 (83%)
Frame = -2
Query: 607 FMVISPLSMPVKRTRELRALNGWRYYFSLFLEFSDITMPLVRVVFSKICSAISFFLVCLV 428
F IS SMPVKRT+ELRAL GWRYYFSL+LE SDI MPLVR V K+ +AISFFLV L+
Sbjct: 395 FSAISHFSMPVKRTKELRALTGWRYYFSLYLELSDIMMPLVRAVIDKVSNAISFFLVTLI 454
Query: 427 GRSLGLIYSGIRQSLGWR 374
GRSLGLIY+GIRQSL W+
Sbjct: 455 GRSLGLIYTGIRQSLRWK 472
>ref|NP_568703.1| uncharacterized protein [Arabidopsis thaliana]
gi|332008347|gb|AED95730.1| uncharacterized protein
[Arabidopsis thaliana]
Length = 511
Score = 194 bits (494), Expect(2) = 2e-56
Identities = 102/230 (44%), Positives = 154/230 (66%)
Frame = -1
Query: 1463 SDQQVNVEYAWAEVKSKLLGALDAAKQDGGLESRVVESGDYSTKCPLSLYAVADGPRLRL 1284
+D + +++ W VK KLL +LDA K++ L S+V++ K PLSLYA+++GP+L L
Sbjct: 143 TDFEGDLKSVWDVVKVKLLDSLDAIKRENTLGSKVLKPKPPQGKPPLSLYAISEGPQLYL 202
Query: 1283 LWTALQQLEKEVNNISGSYQVVDRGDWMGTSSEVLQISSQSVFVTWLEEELNLECKKHDK 1104
LW+ Q+LE+E N ISG+ ++ +WMG+ +++++ + Q+ WL+EEL +E D
Sbjct: 203 LWSCFQKLEEETNKISGT---INSDEWMGSFTQIVREAYQAACTAWLKEELYVENTDSDN 259
Query: 1103 NILLMMLQKLKQDDNITKNIKRSGKDELYADLLYFLRFRFLSTGCRYDNKLLAHHGVDIL 924
I ++++ L + D I I++SGK++L+A+ LYF +F YD HGV IL
Sbjct: 260 AITPLLIRMLNEKDAIFDKIRKSGKEDLFAEFLYFHKFGSPGKAFCYDLSFFRTHGVAIL 319
Query: 923 EDLVITLADGITTNYLELISVDSNMSSDMNRMGLNLCSLSTRALQKLRNE 774
ED +ITLADG+ + YLELISVDS S++MN GL++CSLS+RALQKLRNE
Sbjct: 320 EDFMITLADGVASIYLELISVDSKFSNEMNSGGLSICSLSSRALQKLRNE 369
Score = 53.5 bits (127), Expect(2) = 2e-56
Identities = 26/62 (41%), Positives = 34/62 (54%)
Frame = -2
Query: 1729 FNAHISTILERSANLKDSSMRCCCFGTLISPDNATVPNWVPVLDQALMMFSIVLAYIAGV 1550
FN S L S + DS+ +C CF +L D WVP+ DQ L+ SI L Y+AGV
Sbjct: 51 FNVSTSLNLGSSCSQGDSTCKCTCFASLADFDGVAGSGWVPIGDQVLLTASIFLTYMAGV 110
Query: 1549 VP 1544
+P
Sbjct: 111 IP 112
Score = 121 bits (303), Expect = 8e-25
Identities = 55/81 (67%), Positives = 70/81 (86%)
Frame = -2
Query: 616 PLQFMVISPLSMPVKRTRELRALNGWRYYFSLFLEFSDITMPLVRVVFSKICSAISFFLV 437
PL++ +IS S+PVKRT+EL+AL+GWRYYFSLFLE SDI MP++RVV K+ S ISFFLV
Sbjct: 431 PLRYSIISDFSLPVKRTKELKALSGWRYYFSLFLELSDIGMPIIRVVLDKVSSVISFFLV 490
Query: 436 CLVGRSLGLIYSGIRQSLGWR 374
L+GRS+GLI++GIRQSL W+
Sbjct: 491 TLIGRSVGLIFTGIRQSLRWK 511