BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00012286
         (1491 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinif...   684   0.0  
emb|CBI21489.3| unnamed protein product [Vitis vinifera]              661   0.0  
ref|XP_003524281.1| PREDICTED: cytochrome P450 71A1-like [Glycin...   651   0.0  
ref|XP_002532045.1| flavonoid 3-hydroxylase, putative [Ricinus c...   650   0.0  
ref|XP_002310016.1| cytochrome P450 [Populus trichocarpa] gi|222...   650   0.0  

>ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinifera]
          Length = 498

 Score =  684 bits (1766), Expect = 0.0
 Identities = 328/465 (70%), Positives = 396/465 (85%), Gaps = 1/465 (0%)
 Frame = -2

Query: 1490 PPGPKGIPVLGNLLMLGELPHRDLHHLSKQYGSIMYLRLGFIPTIVVSSPQAAELFLKTY 1311
            PPGP+GIP+LGNL MLGELPH+DL  L+K+YG IMY+R   +PTIVVSSPQAAE FLKT 
Sbjct: 32   PPGPRGIPILGNLHMLGELPHQDLLRLAKKYGPIMYMRFALVPTIVVSSPQAAEQFLKTN 91

Query: 1310 DLNFASRPFNEAAKYISYERKGLSFAEYGSYWRNVRKLCTLELLSNLKIESFKTMRREEL 1131
            DL FA RP +E ++ +SY+RKG+SF +YG YWRN+RKLCTL LLSNL+I SF+ +RREEL
Sbjct: 92   DLVFAGRPPHEGSRIVSYDRKGISFTDYGPYWRNMRKLCTLGLLSNLRISSFQPLRREEL 151

Query: 1130 GLFIESLKEASRDHVEVDLSAKVLTLSTDMSCLMVFGKKRIHNEHEQKGFHAVVQEGMQL 951
             L I+SLKEA+     VDLSAK+ +LS DMSC M+FGKK +  + +++GF AV+QEGMQL
Sbjct: 152  DLLIKSLKEAALARTAVDLSAKISSLSADMSCRMIFGKKYMDKDIDERGFKAVIQEGMQL 211

Query: 950  AALFNIADYIPYVGALDLQGLAKRMKVVSKVFDDMFEQIIDEHV-DAGDKGQHRDFVDVM 774
            A   NI DYIP+V  LDLQGLA+RMK +SKVFD  FE+IID+H+ +  ++GQ +D +DVM
Sbjct: 212  AGAPNIGDYIPFVAPLDLQGLARRMKAISKVFDAFFEKIIDDHIHEPKEEGQPKDLIDVM 271

Query: 773  LSFMESKENEFQIERSNIKAIILDMLVGSMDTSSTAIEWVFSELLKHPNVMKKVQQEIER 594
            L +M SKENEFQIERSNIKA++LDML GSMDTS+TAIEW  +ELLK+P +MKKVQ+E+E+
Sbjct: 272  LGYMGSKENEFQIERSNIKALVLDMLAGSMDTSATAIEWALAELLKNPRIMKKVQEELEK 331

Query: 593  VVGQDRLVEESDLTNLEYLDMVVKEALRLHPVAPLLIPHESIEDCTVNGYHIPQKSRIIV 414
            VVG +R VEESDL +LEYLDMVVKE LRLHPVAPLLIPHES+EDCTVNG+HIPQKSR++V
Sbjct: 332  VVGMERKVEESDLESLEYLDMVVKETLRLHPVAPLLIPHESLEDCTVNGFHIPQKSRVMV 391

Query: 413  NTWAIGRDPSVWDDAENFIPERFIGSDIEYKGRDFQLLPFGQGRRGCPGMQLGLTVVRLV 234
            NT+AIGRDP+VW DAE F+PERFIGS I+ +GRDFQL+PFG GRRGCPGMQLGLTVVRLV
Sbjct: 392  NTYAIGRDPNVWTDAEKFLPERFIGSSIDLRGRDFQLIPFGSGRRGCPGMQLGLTVVRLV 451

Query: 233  AAQLVHCFDWELPNGMQCTELNMDEEFGLTMPRAKHLSVIPTFRL 99
             AQLVHCFDWELPNGM  +EL+M EEFGLT+PRAKH+  +PT+RL
Sbjct: 452  LAQLVHCFDWELPNGMMPSELDMTEEFGLTVPRAKHILAVPTYRL 496


>emb|CBI21489.3| unnamed protein product [Vitis vinifera]
          Length = 563

 Score =  661 bits (1705), Expect = 0.0
 Identities = 324/482 (67%), Positives = 390/482 (80%), Gaps = 26/482 (5%)
 Frame = -2

Query: 1490 PPGPKGIPVLGNLLMLGELPHRDLHHLSKQYGSIMYLRLGFIPTIVVSSPQAAELFLKTY 1311
            PPGP+GIP+LGNL MLGELPH+DL  L+K+YG IMY+R   +PTIVVSSPQAAE FLKT 
Sbjct: 60   PPGPRGIPILGNLHMLGELPHQDLLRLAKKYGPIMYMRFALVPTIVVSSPQAAEQFLKTN 119

Query: 1310 DLNFASRPFNEAAKYISYERKGLSFAEYGSYWRNVRKLCTLELLSNLKIESFKTMRREEL 1131
            DL FA RP +E ++ +SY+RKG+SF +YG YWRN+RKLCTL LLSNL+I SF+ +RREEL
Sbjct: 120  DLVFAGRPPHEGSRIVSYDRKGISFTDYGPYWRNMRKLCTLGLLSNLRISSFQPLRREEL 179

Query: 1130 GLFIESLKEASRDHVEVDLSAKVLTLSTDMSCLMVFGKKRIHNEHEQKGFHAVVQEGMQL 951
             L I+SLKEA+     VDLSAK+ +LS DMSC M+FGKK +  + +++GF AV+QEGMQL
Sbjct: 180  DLLIKSLKEAALARTAVDLSAKISSLSADMSCRMIFGKKYMDKDIDERGFKAVIQEGMQL 239

Query: 950  AALFNIADYIPYVGALDLQGLAKRMKVVSKVFDDMFEQIIDEHV-DAGDKGQHRDFVDVM 774
            A   NI DYIP+V  LDLQGLA+RMK +SKVFD  FE+IID+H+ +  ++GQ +D +DVM
Sbjct: 240  AGAPNIGDYIPFVAPLDLQGLARRMKAISKVFDAFFEKIIDDHIHEPKEEGQPKDLIDVM 299

Query: 773  LSFMESKENEFQIERSNIKAIIL-------------------------DMLVGSMDTSST 669
            L +M SKENEFQIERSNIKA++L                         DML GSMDTS+T
Sbjct: 300  LGYMGSKENEFQIERSNIKALVLVSIHTLIIHISKPSSSQYICCLNFQDMLAGSMDTSAT 359

Query: 668  AIEWVFSELLKHPNVMKKVQQEIERVVGQDRLVEESDLTNLEYLDMVVKEALRLHPVAPL 489
            AIEW  +ELLK+P +MKKVQ+E+E+VVG +R VEESDL +LEYLDMVVKE LRLHPVAPL
Sbjct: 360  AIEWALAELLKNPRIMKKVQEELEKVVGMERKVEESDLESLEYLDMVVKETLRLHPVAPL 419

Query: 488  LIPHESIEDCTVNGYHIPQKSRIIVNTWAIGRDPSVWDDAENFIPERFIGSDIEYKGRDF 309
            LIPHES+EDCTVNG+HIPQKSR++VNT+AIGRDP+VW DAE F+PERFIGS I+ +GRDF
Sbjct: 420  LIPHESLEDCTVNGFHIPQKSRVMVNTYAIGRDPNVWTDAEKFLPERFIGSSIDLRGRDF 479

Query: 308  QLLPFGQGRRGCPGMQLGLTVVRLVAAQLVHCFDWELPNGMQCTELNMDEEFGLTMPRAK 129
            QL+PFG GRRGCPGMQLGLTVVRLV AQLVHCFDWELPNGM  +EL+M EEFGLT+PRAK
Sbjct: 480  QLIPFGSGRRGCPGMQLGLTVVRLVLAQLVHCFDWELPNGMMPSELDMTEEFGLTVPRAK 539

Query: 128  HL 123
            H+
Sbjct: 540  HI 541


>ref|XP_003524281.1| PREDICTED: cytochrome P450 71A1-like [Glycine max]
          Length = 536

 Score =  651 bits (1679), Expect = 0.0
 Identities = 321/466 (68%), Positives = 384/466 (82%), Gaps = 2/466 (0%)
 Frame = -2

Query: 1490 PPGPKGIPVLGNLLMLGELPHRDLHHLSKQYGSIMYLRLGFIPTIVVSSPQAAELFLKTY 1311
            PPGP+G+P+LG+L  LG  PHRDLH L+++YG +M+LRLGF+PTIVVSSPQAAELFLKT+
Sbjct: 67   PPGPRGLPILGSLHKLGPNPHRDLHQLAQKYGPVMHLRLGFVPTIVVSSPQAAELFLKTH 126

Query: 1310 DLNFASRPFNEAAKYISYERKGLSFAEYGSYWRNVRKLCTLELLSNLKIESFKTMRREEL 1131
            DL FASRP  EAAKYIS+E++ LSFAEYGSYWRNVRK+CTLELLS+ KI SF++MR EEL
Sbjct: 127  DLVFASRPPLEAAKYISWEQRNLSFAEYGSYWRNVRKMCTLELLSHTKINSFRSMREEEL 186

Query: 1130 GLFIESLKEASRDHVEVDLSAKVLTLSTDMSCLMVFGKKRIHNEHEQKGFHAVVQEGMQL 951
             L ++ L+EA++D   VDLSAKV TLS DMSC MV GKK +  + ++KGF AV+QEGM L
Sbjct: 187  DLMVKLLREAAKDGAVVDLSAKVSTLSADMSCRMVLGKKYMDRDLDEKGFKAVMQEGMHL 246

Query: 950  AALFNIADYIPYVGALDLQGLAKRMKVVSKVFDDMFEQIIDEHVDAGDKGQHR--DFVDV 777
            AA  N+ DYIPY+ ALDLQGL KRMKVV K+FDD FE+IIDEH+ + +KG+ R  DFVDV
Sbjct: 247  AATPNMGDYIPYIAALDLQGLTKRMKVVGKIFDDFFEKIIDEHLQS-EKGEDRTKDFVDV 305

Query: 776  MLSFMESKENEFQIERSNIKAIILDMLVGSMDTSSTAIEWVFSELLKHPNVMKKVQQEIE 597
            ML F+ ++E+E++IER NIKAI+LDML GSMDTS+TAIEW  SELLK+P VMKKVQ E+E
Sbjct: 306  MLDFVGTEESEYRIERPNIKAILLDMLAGSMDTSATAIEWTLSELLKNPRVMKKVQMELE 365

Query: 596  RVVGQDRLVEESDLTNLEYLDMVVKEALRLHPVAPLLIPHESIEDCTVNGYHIPQKSRII 417
             VVG  R VEESDL  L YLDMVVKE++RLHPVAPLLIPH+S EDC V    IP+KSR+I
Sbjct: 366  TVVGMKRKVEESDLDKLVYLDMVVKESMRLHPVAPLLIPHQSTEDCMVGDLFIPKKSRVI 425

Query: 416  VNTWAIGRDPSVWDDAENFIPERFIGSDIEYKGRDFQLLPFGQGRRGCPGMQLGLTVVRL 237
            VN WAI RDPS WD+AE F PERF GS I+ +GRDF+L+PFG GRRGCPG+QLGLTVVRL
Sbjct: 426  VNAWAIMRDPSAWDEAEKFWPERFEGSSIDVRGRDFELIPFGSGRRGCPGLQLGLTVVRL 485

Query: 236  VAAQLVHCFDWELPNGMQCTELNMDEEFGLTMPRAKHLSVIPTFRL 99
              AQ+VHCFDW+LP  +   +L+M EEFGLTMPRA HL  IPT+RL
Sbjct: 486  TVAQIVHCFDWKLPKDILPDDLDMKEEFGLTMPRANHLHAIPTYRL 531


>ref|XP_002532045.1| flavonoid 3-hydroxylase, putative [Ricinus communis]
            gi|223528288|gb|EEF30335.1| flavonoid 3-hydroxylase,
            putative [Ricinus communis]
          Length = 505

 Score =  650 bits (1678), Expect = 0.0
 Identities = 314/466 (67%), Positives = 379/466 (81%)
 Frame = -2

Query: 1490 PPGPKGIPVLGNLLMLGELPHRDLHHLSKQYGSIMYLRLGFIPTIVVSSPQAAELFLKTY 1311
            PPGP+G P+ G+L +LG+ PHR+LH L+++YG IM+LRLG + TIVVSSPQAAE FLKT+
Sbjct: 40   PPGPRGFPIFGSLHLLGKYPHRELHRLAQKYGPIMHLRLGLVSTIVVSSPQAAESFLKTH 99

Query: 1310 DLNFASRPFNEAAKYISYERKGLSFAEYGSYWRNVRKLCTLELLSNLKIESFKTMRREEL 1131
            DL FASRP ++AAK+ISYE+K LSFA YGSYWRNVRK+CTLELLSN+K+ SFK+MR+EEL
Sbjct: 100  DLAFASRPPHQAAKFISYEQKNLSFAPYGSYWRNVRKMCTLELLSNVKVNSFKSMRKEEL 159

Query: 1130 GLFIESLKEASRDHVEVDLSAKVLTLSTDMSCLMVFGKKRIHNEHEQKGFHAVVQEGMQL 951
             L I+ +K AS   V VDLSAKV +LS DMSC MVFGKK +  E +++GF AV+QEGM L
Sbjct: 160  DLLIDCIKNASCQRVAVDLSAKVASLSADMSCRMVFGKKYMDKEFDERGFKAVIQEGMHL 219

Query: 950  AALFNIADYIPYVGALDLQGLAKRMKVVSKVFDDMFEQIIDEHVDAGDKGQHRDFVDVML 771
             A  N+ +YIP +  LDLQGL KRMK VSKVFD  FE+IIDEH++  D+ + +DFVDVML
Sbjct: 220  GAAPNLGNYIPQIAGLDLQGLTKRMKAVSKVFDSFFEKIIDEHMEFKDENRTKDFVDVML 279

Query: 770  SFMESKENEFQIERSNIKAIILDMLVGSMDTSSTAIEWVFSELLKHPNVMKKVQQEIERV 591
             FM S+ +E+ I R NIKAIILDML GSMDTS+  IEW  SELLKHP VMKKVQ+E+E  
Sbjct: 280  GFMGSEVSEYHIGRDNIKAIILDMLAGSMDTSAAVIEWALSELLKHPGVMKKVQKELEEK 339

Query: 590  VGQDRLVEESDLTNLEYLDMVVKEALRLHPVAPLLIPHESIEDCTVNGYHIPQKSRIIVN 411
            VG  R+VEESD+  LEYL+MV+KE  RLHPVAPLL+PHE+ ED T++GY IP+KS II+N
Sbjct: 340  VGMTRMVEESDVEKLEYLEMVIKETFRLHPVAPLLLPHEAAEDTTIDGYLIPKKSHIIIN 399

Query: 410  TWAIGRDPSVWDDAENFIPERFIGSDIEYKGRDFQLLPFGQGRRGCPGMQLGLTVVRLVA 231
            T+AIGRDPS W +AE F+PERF+G +I+ +GRDFQLLPFG GRRGCPG+QLG+TVVRLV 
Sbjct: 400  TFAIGRDPSAWTEAEKFLPERFLGRNIDIRGRDFQLLPFGAGRRGCPGIQLGMTVVRLVV 459

Query: 230  AQLVHCFDWELPNGMQCTELNMDEEFGLTMPRAKHLSVIPTFRLRI 93
            AQLVHCFDWELPNGM  TEL+M EEF L  PRAKHL  IP +RL I
Sbjct: 460  AQLVHCFDWELPNGMLPTELDMTEEFSLVTPRAKHLEAIPNYRLHI 505


>ref|XP_002310016.1| cytochrome P450 [Populus trichocarpa] gi|222852919|gb|EEE90466.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 497

 Score =  650 bits (1677), Expect = 0.0
 Identities = 315/466 (67%), Positives = 373/466 (80%)
 Frame = -2

Query: 1490 PPGPKGIPVLGNLLMLGELPHRDLHHLSKQYGSIMYLRLGFIPTIVVSSPQAAELFLKTY 1311
            PPGP G P+ G+L +LG+ PH DLH L+K+YG IMY+RLG +PT+VVSSP+AAEL LKT 
Sbjct: 32   PPGPIGFPIFGSLHLLGKFPHHDLHQLAKKYGPIMYMRLGLVPTVVVSSPRAAELILKTN 91

Query: 1310 DLNFASRPFNEAAKYISYERKGLSFAEYGSYWRNVRKLCTLELLSNLKIESFKTMRREEL 1131
            DL FASRP NEAAK+ISYE+K LSFA YGSYWRNVRK+CTLELLSN KI SF + R+EEL
Sbjct: 92   DLVFASRPRNEAAKHISYEQKNLSFAPYGSYWRNVRKMCTLELLSNHKINSFMSTRKEEL 151

Query: 1130 GLFIESLKEASRDHVEVDLSAKVLTLSTDMSCLMVFGKKRIHNEHEQKGFHAVVQEGMQL 951
             L I+ +K+ASR+ V VDL AKV +LS D+SC MVFGKK +  E ++KGF  V  E M+L
Sbjct: 152  DLLIDYIKDASRERVAVDLGAKVSSLSADISCRMVFGKKYLEKEFDEKGFKPVTHELMRL 211

Query: 950  AALFNIADYIPYVGALDLQGLAKRMKVVSKVFDDMFEQIIDEHVDAGDKGQHRDFVDVML 771
            AA FN+ DYIP +  LDLQGL KRMK V KVFDD FE+IIDEH+   D+ + +DFVDVML
Sbjct: 212  AASFNLGDYIPPIAPLDLQGLTKRMKAVGKVFDDFFEKIIDEHIQFKDENRTKDFVDVML 271

Query: 770  SFMESKENEFQIERSNIKAIILDMLVGSMDTSSTAIEWVFSELLKHPNVMKKVQQEIERV 591
             F+ S+E E++I R NIKAIILDMLVGSMDTS+TAIEW  SEL++HP VMKKVQ+E+E  
Sbjct: 272  DFLGSEETEYRIGRDNIKAIILDMLVGSMDTSATAIEWTLSELIRHPRVMKKVQKELEEK 331

Query: 590  VGQDRLVEESDLTNLEYLDMVVKEALRLHPVAPLLIPHESIEDCTVNGYHIPQKSRIIVN 411
            +G DR+VEESDL  LEYL MV+KEA RLHPVAPLL PHES+EDCT++G+ IPQK+R+IVN
Sbjct: 332  IGMDRMVEESDLEGLEYLHMVIKEAFRLHPVAPLLGPHESMEDCTIDGFLIPQKTRVIVN 391

Query: 410  TWAIGRDPSVWDDAENFIPERFIGSDIEYKGRDFQLLPFGQGRRGCPGMQLGLTVVRLVA 231
             WAIGRD S W DA  FIPERF GS+I+ +GRDFQLLPFG GRRGCPGM LGLT+VR + 
Sbjct: 392  VWAIGRDQSAWTDANKFIPERFAGSNIDVRGRDFQLLPFGAGRRGCPGMHLGLTMVRQIV 451

Query: 230  AQLVHCFDWELPNGMQCTELNMDEEFGLTMPRAKHLSVIPTFRLRI 93
            AQLVHCFDWELPN M   EL+M E FGL  PRA HL   PT+RL +
Sbjct: 452  AQLVHCFDWELPNNMLPEELDMTEAFGLVTPRANHLCATPTYRLHL 497