BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00012116
         (1047 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAF19230.1|AC007505_6 Putative biotin holocarboxylase synthet...   381   e-103
ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thali...   381   e-103
ref|NP_850958.1| holocarboxylase synthetase 2 [Arabidopsis thali...   381   e-103
ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis ly...   381   e-103
emb|CBI19072.3| unnamed protein product [Vitis vinifera]              380   e-103

>gb|AAF19230.1|AC007505_6 Putative biotin holocarboxylase synthetase [Arabidopsis thaliana]
          Length = 310

 Score =  381 bits (979), Expect = e-103
 Identities = 184/281 (65%), Positives = 233/281 (82%), Gaps = 2/281 (0%)
 Frame = -3

Query: 838 LILHGKSSFETELAQSLKTNNTIKLPEEDAQVSILLHSSELRGVHND--AFDVDLYMNSL 665
           L+L+GKSS ET+ A  LK NN +KLP+ +++VSI L S     V +D  +F++ L+MNS+
Sbjct: 9   LVLYGKSSVETDTATRLKNNNVLKLPD-NSKVSIFLQSEIKNLVRDDDSSFNLSLFMNSI 67

Query: 664 TTHRYGKFFIYSPRLSSTQDIVSKNFSEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLF 485
           +THR+G+F I+SP LSST D+VS NFSEIPVGSVCV+D+Q KGRGR+KN+WESPKGCL++
Sbjct: 68  STHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMY 127

Query: 484 SFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGIL 305
           SFT+EMEDGRVVPL+QYVV LA+ EA+KDVCD KG ++ DV+IKWPNDLYLNG+K+GGIL
Sbjct: 128 SFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGIL 187

Query: 304 CTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFY 125
           CTSTYRS+KF VSVG+GLNVDNE PTTCLN VL ++      L+REEIL AFFK+FENF+
Sbjct: 188 CTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFF 247

Query: 124 HVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESVDEV 2
            +F+ QGF++LEE+Y R WLHSGQRV+ +EK NED+ V  V
Sbjct: 248 DLFMEQGFKSLEELYYRTWLHSGQRVIAEEK-NEDQVVQNV 287


>ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thaliana]
           gi|332193776|gb|AEE31897.1| holocarboxylase synthetase 2
           [Arabidopsis thaliana]
          Length = 329

 Score =  381 bits (979), Expect = e-103
 Identities = 184/281 (65%), Positives = 233/281 (82%), Gaps = 2/281 (0%)
 Frame = -3

Query: 838 LILHGKSSFETELAQSLKTNNTIKLPEEDAQVSILLHSSELRGVHND--AFDVDLYMNSL 665
           L+L+GKSS ET+ A  LK NN +KLP+ +++VSI L S     V +D  +F++ L+MNS+
Sbjct: 9   LVLYGKSSVETDTATRLKNNNVLKLPD-NSKVSIFLQSEIKNLVRDDDSSFNLSLFMNSI 67

Query: 664 TTHRYGKFFIYSPRLSSTQDIVSKNFSEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLF 485
           +THR+G+F I+SP LSST D+VS NFSEIPVGSVCV+D+Q KGRGR+KN+WESPKGCL++
Sbjct: 68  STHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMY 127

Query: 484 SFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGIL 305
           SFT+EMEDGRVVPL+QYVV LA+ EA+KDVCD KG ++ DV+IKWPNDLYLNG+K+GGIL
Sbjct: 128 SFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGIL 187

Query: 304 CTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFY 125
           CTSTYRS+KF VSVG+GLNVDNE PTTCLN VL ++      L+REEIL AFFK+FENF+
Sbjct: 188 CTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFF 247

Query: 124 HVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESVDEV 2
            +F+ QGF++LEE+Y R WLHSGQRV+ +EK NED+ V  V
Sbjct: 248 DLFMEQGFKSLEELYYRTWLHSGQRVIAEEK-NEDQVVQNV 287


>ref|NP_850958.1| holocarboxylase synthetase 2 [Arabidopsis thaliana]
           gi|19698373|gb|AAL93112.1|AF414941_1 holocarboxylase
           synthetase hcs2.d [Arabidopsis thaliana]
           gi|332193775|gb|AEE31896.1| holocarboxylase synthetase 2
           [Arabidopsis thaliana]
          Length = 297

 Score =  381 bits (979), Expect = e-103
 Identities = 184/281 (65%), Positives = 233/281 (82%), Gaps = 2/281 (0%)
 Frame = -3

Query: 838 LILHGKSSFETELAQSLKTNNTIKLPEEDAQVSILLHSSELRGVHND--AFDVDLYMNSL 665
           L+L+GKSS ET+ A  LK NN +KLP+ +++VSI L S     V +D  +F++ L+MNS+
Sbjct: 9   LVLYGKSSVETDTATRLKNNNVLKLPD-NSKVSIFLQSEIKNLVRDDDSSFNLSLFMNSI 67

Query: 664 TTHRYGKFFIYSPRLSSTQDIVSKNFSEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLF 485
           +THR+G+F I+SP LSST D+VS NFSEIPVGSVCV+D+Q KGRGR+KN+WESPKGCL++
Sbjct: 68  STHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMY 127

Query: 484 SFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGIL 305
           SFT+EMEDGRVVPL+QYVV LA+ EA+KDVCD KG ++ DV+IKWPNDLYLNG+K+GGIL
Sbjct: 128 SFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGIL 187

Query: 304 CTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFY 125
           CTSTYRS+KF VSVG+GLNVDNE PTTCLN VL ++      L+REEIL AFFK+FENF+
Sbjct: 188 CTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFF 247

Query: 124 HVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESVDEV 2
            +F+ QGF++LEE+Y R WLHSGQRV+ +EK NED+ V  V
Sbjct: 248 DLFMEQGFKSLEELYYRTWLHSGQRVIAEEK-NEDQVVQNV 287


>ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis lyrata subsp. lyrata]
           gi|297339768|gb|EFH70185.1| holocarboxylase synthetase 2
           [Arabidopsis lyrata subsp. lyrata]
          Length = 328

 Score =  381 bits (978), Expect = e-103
 Identities = 186/281 (66%), Positives = 233/281 (82%), Gaps = 2/281 (0%)
 Frame = -3

Query: 838 LILHGKSSFETELAQSLKTNNTIKLPEEDAQVSILLHSSELRGV--HNDAFDVDLYMNSL 665
           L+L GKSS ET+ A  LK NN +KLP+ + +VSI L S E++ +   +D+F++ L+MNS+
Sbjct: 9   LVLCGKSSVETDAATRLKNNNVLKLPD-NTKVSIFLQS-EIKNLVKDDDSFNLSLFMNSI 66

Query: 664 TTHRYGKFFIYSPRLSSTQDIVSKNFSEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLF 485
           +THR+G+F I+SP LSST D+VS NFSEIPVGSVCV D+QFKGRGR+KN+WESPKGCL++
Sbjct: 67  STHRFGRFLIWSPFLSSTHDVVSHNFSEIPVGSVCVTDIQFKGRGRTKNVWESPKGCLMY 126

Query: 484 SFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGIL 305
           SFTVEMEDGRVVPL+QYVV LA+ EA+KDVCD KG ++IDV+IKWPNDLYLNG+KVGGIL
Sbjct: 127 SFTVEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLNGLKVGGIL 186

Query: 304 CTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFY 125
           CTSTY S+KF+VSVG+GLNVDNE PTTCLN VL E       L+REEIL AFF +FE F+
Sbjct: 187 CTSTYGSRKFHVSVGVGLNVDNEKPTTCLNAVLKENCPASNLLKREEILGAFFHKFETFF 246

Query: 124 HVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESVDEV 2
            +F+ QGF++LEE+Y R WLHSGQRV+++EK NED+ V  V
Sbjct: 247 DLFMEQGFKSLEELYYRTWLHSGQRVIVEEK-NEDQVVQNV 286


>emb|CBI19072.3| unnamed protein product [Vitis vinifera]
          Length = 416

 Score =  380 bits (976), Expect = e-103
 Identities = 183/281 (65%), Positives = 231/281 (82%)
 Frame = -3

Query: 844 NLLILHGKSSFETELAQSLKTNNTIKLPEEDAQVSILLHSSELRGVHNDAFDVDLYMNSL 665
           +LL+L GKSS E E A+SLK  NT+KLP+ + +VSILLH  E+     + F +DL+M+SL
Sbjct: 96  SLLVLCGKSSAEIEFAKSLKAKNTLKLPD-NGEVSILLHP-EIEKPEEEPFQMDLFMSSL 153

Query: 664 TTHRYGKFFIYSPRLSSTQDIVSKNFSEIPVGSVCVADLQFKGRGRSKNLWESPKGCLLF 485
           +T+ +G+F I+SP+L ST D+VS+NF E+P+G+VCVAD+Q KGRGRSKN WESP+GCLLF
Sbjct: 154 STNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPRGCLLF 213

Query: 484 SFTVEMEDGRVVPLLQYVVCLAMVEAIKDVCDVKGFAFIDVRIKWPNDLYLNGVKVGGIL 305
           SFT++MEDGR VPLLQYVV LA+ EAIK +CD KG   +DV+IKWPNDLYLNG+KVGGIL
Sbjct: 214 SFTLQMEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLKVGGIL 273

Query: 304 CTSTYRSKKFNVSVGIGLNVDNEMPTTCLNKVLSELSMMDCKLRREEILAAFFKRFENFY 125
           CTSTY+S+KFNVS GIGLNVDNE PTTCLN VL ELS +  + RRE+I+AAFF +FE F+
Sbjct: 274 CTSTYKSRKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAFFNKFETFF 333

Query: 124 HVFINQGFRALEEMYCRVWLHSGQRVVIQEKNNEDESVDEV 2
            +FINQGF+ LEE+YC+ WLHSGQRV++QE N E + V+ V
Sbjct: 334 SLFINQGFQTLEELYCKTWLHSGQRVIVQE-NIEGQMVENV 373