BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00012006
         (1589 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002269614.1| PREDICTED: L-ascorbate oxidase homolog [Viti...   625   0.0  
ref|XP_002272880.1| PREDICTED: L-ascorbate oxidase homolog [Viti...   605   0.0  
ref|XP_003596958.1| L-ascorbate oxidase-like protein [Medicago t...   596   0.0  
ref|XP_003596959.1| L-ascorbate oxidase-like protein [Medicago t...   596   0.0  
ref|XP_002332613.1| predicted protein [Populus trichocarpa] gi|2...   592   0.0  

>ref|XP_002269614.1| PREDICTED: L-ascorbate oxidase homolog [Vitis vinifera]
            gi|297739160|emb|CBI28811.3| unnamed protein product
            [Vitis vinifera]
          Length = 541

 Score =  625 bits (1612), Expect(2) = 0.0
 Identities = 299/346 (86%), Positives = 319/346 (92%)
 Frame = +1

Query: 319  RGWNGYTFTVSQGKTYRFRISNVGIATSFNFRIQGHTMKLVEVEGSHTLQNTYSSLDVHL 498
            RG NGYTFTV QGKTYRFRISNVGIATS NFRIQGH M LVEVEG+H LQNTYSSLD+HL
Sbjct: 196  RGSNGYTFTVDQGKTYRFRISNVGIATSLNFRIQGHKMLLVEVEGTHALQNTYSSLDIHL 255

Query: 499  GQSYSVLVTADQPAQDYYIVVSSRFTDPVLTNTAILHYSNSAGRVSGPPPGGPTTQIDWS 678
            GQSYSVLVTADQPAQDYYIVVS+RFT  VLT T+ LHYSNSAGRVSGPPPGGPT Q DWS
Sbjct: 256  GQSYSVLVTADQPAQDYYIVVSTRFTSQVLTTTSTLHYSNSAGRVSGPPPGGPTIQTDWS 315

Query: 679  LNQARSIRWNLTASGPRPNPQGSYHYGLINTTRTIRLANSAPVINGKQRYAVNSVSFIPA 858
            LNQARSIR NLTASGPRPNPQGSYHYGLINTT TIRLANSAPVINGKQRYAVNSVSFIPA
Sbjct: 316  LNQARSIRRNLTASGPRPNPQGSYHYGLINTTHTIRLANSAPVINGKQRYAVNSVSFIPA 375

Query: 859  DTPLKLADYFKISGVFSVGSISDNPTGGGGYLQTSVMGADFRAFIEIVFENSEDTVQSWH 1038
            DTPLKLADYFKISGVF++GSI DNPTGGGGYLQTSVM ADFR F E+VFEN EDT+QSWH
Sbjct: 376  DTPLKLADYFKISGVFNLGSIPDNPTGGGGYLQTSVMAADFRGFAEVVFENPEDTLQSWH 435

Query: 1039 VDGYAFFVVGMDGGQWSAASRLNYNLRDTVARSTVQVYPKSWSAIYVGLDNVGMWNIRSE 1218
            +DG+ FFVVGMDGGQW+ +SRL+YNLRDT++R TVQVYPKSW+A+Y+ LDNVGMWN+RSE
Sbjct: 436  IDGHNFFVVGMDGGQWTPSSRLSYNLRDTISRCTVQVYPKSWTALYMPLDNVGMWNVRSE 495

Query: 1219 DWARQYLGQQFYLRVYSAVESWRDEYPIPKNALLCGRASGRTTRPL 1356
            +WARQYLGQQFYLRVYS   SWRDEYPIPKNALLCGRASGR TRPL
Sbjct: 496  NWARQYLGQQFYLRVYSPANSWRDEYPIPKNALLCGRASGRKTRPL 541



 Score =  132 bits (332), Expect(2) = 0.0
 Identities = 60/70 (85%), Positives = 64/70 (91%)
 Frame = +3

Query: 3   QIGSFFYFPSLAFHKAAGGFGGITISSRPRIPVPFPSPDGDFTILAGDWFKKNHTDLKAT 182
           QIGS+FYFPSLAFHKAAGG+GGI ISSRP IPVPFP P GD+TILAGDW+KKNHTDLKA 
Sbjct: 119 QIGSYFYFPSLAFHKAAGGYGGIKISSRPLIPVPFPPPAGDYTILAGDWYKKNHTDLKAI 178

Query: 183 LDGGSTLPFP 212
           LDGGS LPFP
Sbjct: 179 LDGGSDLPFP 188


>ref|XP_002272880.1| PREDICTED: L-ascorbate oxidase homolog [Vitis vinifera]
            gi|302144125|emb|CBI23230.3| unnamed protein product
            [Vitis vinifera]
          Length = 540

 Score =  605 bits (1559), Expect(2) = 0.0
 Identities = 286/346 (82%), Positives = 312/346 (90%)
 Frame = +1

Query: 319  RGWNGYTFTVSQGKTYRFRISNVGIATSFNFRIQGHTMKLVEVEGSHTLQNTYSSLDVHL 498
            RGWNGYTF V QGKTYRFRISNVG+ TS NFRIQGH MKLVEVEGSHTLQNTYS+LD+HL
Sbjct: 195  RGWNGYTFQVDQGKTYRFRISNVGLTTSINFRIQGHKMKLVEVEGSHTLQNTYSALDIHL 254

Query: 499  GQSYSVLVTADQPAQDYYIVVSSRFTDPVLTNTAILHYSNSAGRVSGPPPGGPTTQIDWS 678
            GQSYSVLVTADQP QDYY+VVSSRFT PVLT TAILHYS S   +SGPPPGGPT Q+DWS
Sbjct: 255  GQSYSVLVTADQPPQDYYVVVSSRFTSPVLTTTAILHYSWSTKGLSGPPPGGPTIQVDWS 314

Query: 679  LNQARSIRWNLTASGPRPNPQGSYHYGLINTTRTIRLANSAPVINGKQRYAVNSVSFIPA 858
            LNQARSIRWNLTASGPRPNPQGSYHYGLI T+RTI LANSAP+INGKQRYAVNSVSF+PA
Sbjct: 315  LNQARSIRWNLTASGPRPNPQGSYHYGLITTSRTITLANSAPMINGKQRYAVNSVSFVPA 374

Query: 859  DTPLKLADYFKISGVFSVGSISDNPTGGGGYLQTSVMGADFRAFIEIVFENSEDTVQSWH 1038
             TPLKLADYFKI GVF++GSI   PT G GYLQTSVM ADFR+++EIVF+N ED VQSWH
Sbjct: 375  STPLKLADYFKIPGVFNLGSIPSYPTWGNGYLQTSVMHADFRSYVEIVFQNWEDIVQSWH 434

Query: 1039 VDGYAFFVVGMDGGQWSAASRLNYNLRDTVARSTVQVYPKSWSAIYVGLDNVGMWNIRSE 1218
            +DGY+FFV+GMDGGQW+ ASR +YNLRDTVAR TVQVYPK+W+AIY+ LDNVGMWN+RSE
Sbjct: 435  IDGYSFFVMGMDGGQWTPASRASYNLRDTVARCTVQVYPKAWTAIYMALDNVGMWNVRSE 494

Query: 1219 DWARQYLGQQFYLRVYSAVESWRDEYPIPKNALLCGRASGRTTRPL 1356
            +WARQYLGQQFYLRV+S   SWRDE PIPKNALLCGRASGR TRPL
Sbjct: 495  NWARQYLGQQFYLRVFSPANSWRDELPIPKNALLCGRASGRHTRPL 540



 Score =  124 bits (310), Expect(2) = 0.0
 Identities = 56/70 (80%), Positives = 60/70 (85%)
 Frame = +3

Query: 3   QIGSFFYFPSLAFHKAAGGFGGITISSRPRIPVPFPSPDGDFTILAGDWFKKNHTDLKAT 182
           QIGSFFYFPSLAFHKAAGGFGGI I SRPRIPVPFP+P GDFT+LAGDWFK++H  LK  
Sbjct: 118 QIGSFFYFPSLAFHKAAGGFGGIRILSRPRIPVPFPAPSGDFTVLAGDWFKRDHAVLKGI 177

Query: 183 LDGGSTLPFP 212
           LD G  LPFP
Sbjct: 178 LDRGHNLPFP 187


>ref|XP_003596958.1| L-ascorbate oxidase-like protein [Medicago truncatula]
            gi|124360517|gb|ABN08527.1| Multicopper oxidase, type 1
            [Medicago truncatula] gi|355486006|gb|AES67209.1|
            L-ascorbate oxidase-like protein [Medicago truncatula]
          Length = 539

 Score =  596 bits (1537), Expect(2) = 0.0
 Identities = 286/340 (84%), Positives = 305/340 (89%)
 Frame = +1

Query: 319  RGWNGYTFTVSQGKTYRFRISNVGIATSFNFRIQGHTMKLVEVEGSHTLQNTYSSLDVHL 498
            RG N YTFTV QG TYRFRISNVG+ TS NFRIQGH MKLVEVEG HTLQNTY SLD+HL
Sbjct: 199  RGSNAYTFTVDQGNTYRFRISNVGLTTSINFRIQGHKMKLVEVEGIHTLQNTYDSLDIHL 258

Query: 499  GQSYSVLVTADQPAQDYYIVVSSRFTDPVLTNTAILHYSNSAGRVSGPPPGGPTTQIDWS 678
            GQSYSVLVTADQP QDYYIV S+RFT  +LT T+ILHYSNSA  VSGPPPGG T+QIDWS
Sbjct: 259  GQSYSVLVTADQPPQDYYIVASTRFTSQILTATSILHYSNSAKTVSGPPPGGLTSQIDWS 318

Query: 679  LNQARSIRWNLTASGPRPNPQGSYHYGLINTTRTIRLANSAPVINGKQRYAVNSVSFIPA 858
            L QARS+R NLTASGPRPNPQGSYHYGLINTTRTIRL NSAPVINGKQRYAVNSVSFIPA
Sbjct: 319  LEQARSLRRNLTASGPRPNPQGSYHYGLINTTRTIRLENSAPVINGKQRYAVNSVSFIPA 378

Query: 859  DTPLKLADYFKISGVFSVGSISDNPTGGGGYLQTSVMGADFRAFIEIVFENSEDTVQSWH 1038
            DTPLKLADYFKISGVFS+GSI DNPTGGGGYLQTSVM ADFR ++EIVFEN ED+VQSWH
Sbjct: 379  DTPLKLADYFKISGVFSLGSIPDNPTGGGGYLQTSVMAADFRGYVEIVFENPEDSVQSWH 438

Query: 1039 VDGYAFFVVGMDGGQWSAASRLNYNLRDTVARSTVQVYPKSWSAIYVGLDNVGMWNIRSE 1218
            +DG+ FFVVGMDGGQWSAASR NYNL DT++R TVQVYPKSWSA+Y+ LDNVGMWN+RSE
Sbjct: 439  IDGHHFFVVGMDGGQWSAASRSNYNLGDTISRCTVQVYPKSWSAVYMPLDNVGMWNVRSE 498

Query: 1219 DWARQYLGQQFYLRVYSAVESWRDEYPIPKNALLCGRASG 1338
            +WARQYLGQQFYLRVYS   SWRDEYPIP NAL CGRA G
Sbjct: 499  NWARQYLGQQFYLRVYSPANSWRDEYPIPSNALRCGRAVG 538



 Score =  121 bits (303), Expect(2) = 0.0
 Identities = 54/70 (77%), Positives = 59/70 (84%)
 Frame = +3

Query: 3   QIGSFFYFPSLAFHKAAGGFGGITISSRPRIPVPFPSPDGDFTILAGDWFKKNHTDLKAT 182
           QIGS+FYFPSLA HKAAGG+GGI I+SRP IPVPFP P  DFTILAGDW+ +NHTDLKA 
Sbjct: 122 QIGSYFYFPSLALHKAAGGYGGIKINSRPMIPVPFPPPSNDFTILAGDWYNRNHTDLKAI 181

Query: 183 LDGGSTLPFP 212
           LD GS LP P
Sbjct: 182 LDNGSDLPLP 191


>ref|XP_003596959.1| L-ascorbate oxidase-like protein [Medicago truncatula]
            gi|355486007|gb|AES67210.1| L-ascorbate oxidase-like
            protein [Medicago truncatula]
          Length = 458

 Score =  596 bits (1537), Expect(2) = 0.0
 Identities = 286/340 (84%), Positives = 305/340 (89%)
 Frame = +1

Query: 319  RGWNGYTFTVSQGKTYRFRISNVGIATSFNFRIQGHTMKLVEVEGSHTLQNTYSSLDVHL 498
            RG N YTFTV QG TYRFRISNVG+ TS NFRIQGH MKLVEVEG HTLQNTY SLD+HL
Sbjct: 118  RGSNAYTFTVDQGNTYRFRISNVGLTTSINFRIQGHKMKLVEVEGIHTLQNTYDSLDIHL 177

Query: 499  GQSYSVLVTADQPAQDYYIVVSSRFTDPVLTNTAILHYSNSAGRVSGPPPGGPTTQIDWS 678
            GQSYSVLVTADQP QDYYIV S+RFT  +LT T+ILHYSNSA  VSGPPPGG T+QIDWS
Sbjct: 178  GQSYSVLVTADQPPQDYYIVASTRFTSQILTATSILHYSNSAKTVSGPPPGGLTSQIDWS 237

Query: 679  LNQARSIRWNLTASGPRPNPQGSYHYGLINTTRTIRLANSAPVINGKQRYAVNSVSFIPA 858
            L QARS+R NLTASGPRPNPQGSYHYGLINTTRTIRL NSAPVINGKQRYAVNSVSFIPA
Sbjct: 238  LEQARSLRRNLTASGPRPNPQGSYHYGLINTTRTIRLENSAPVINGKQRYAVNSVSFIPA 297

Query: 859  DTPLKLADYFKISGVFSVGSISDNPTGGGGYLQTSVMGADFRAFIEIVFENSEDTVQSWH 1038
            DTPLKLADYFKISGVFS+GSI DNPTGGGGYLQTSVM ADFR ++EIVFEN ED+VQSWH
Sbjct: 298  DTPLKLADYFKISGVFSLGSIPDNPTGGGGYLQTSVMAADFRGYVEIVFENPEDSVQSWH 357

Query: 1039 VDGYAFFVVGMDGGQWSAASRLNYNLRDTVARSTVQVYPKSWSAIYVGLDNVGMWNIRSE 1218
            +DG+ FFVVGMDGGQWSAASR NYNL DT++R TVQVYPKSWSA+Y+ LDNVGMWN+RSE
Sbjct: 358  IDGHHFFVVGMDGGQWSAASRSNYNLGDTISRCTVQVYPKSWSAVYMPLDNVGMWNVRSE 417

Query: 1219 DWARQYLGQQFYLRVYSAVESWRDEYPIPKNALLCGRASG 1338
            +WARQYLGQQFYLRVYS   SWRDEYPIP NAL CGRA G
Sbjct: 418  NWARQYLGQQFYLRVYSPANSWRDEYPIPSNALRCGRAVG 457



 Score =  121 bits (303), Expect(2) = 0.0
 Identities = 54/70 (77%), Positives = 59/70 (84%)
 Frame = +3

Query: 3   QIGSFFYFPSLAFHKAAGGFGGITISSRPRIPVPFPSPDGDFTILAGDWFKKNHTDLKAT 182
           QIGS+FYFPSLA HKAAGG+GGI I+SRP IPVPFP P  DFTILAGDW+ +NHTDLKA 
Sbjct: 41  QIGSYFYFPSLALHKAAGGYGGIKINSRPMIPVPFPPPSNDFTILAGDWYNRNHTDLKAI 100

Query: 183 LDGGSTLPFP 212
           LD GS LP P
Sbjct: 101 LDNGSDLPLP 110


>ref|XP_002332613.1| predicted protein [Populus trichocarpa] gi|222832814|gb|EEE71291.1|
            predicted protein [Populus trichocarpa]
          Length = 544

 Score =  592 bits (1527), Expect(2) = 0.0
 Identities = 284/347 (81%), Positives = 306/347 (88%), Gaps = 1/347 (0%)
 Frame = +1

Query: 319  RGWNGYTFTVSQGKTYRFRISNVGIATSFNFRIQGHTMKLVEVEGSHTLQNTYSSLDVHL 498
            RGWNGYTFTV  GKTYRFRISNVG+ TS NFRIQGH MKLVEVEGSHTLQNTYS LD+HL
Sbjct: 198  RGWNGYTFTVDPGKTYRFRISNVGLTTSINFRIQGHKMKLVEVEGSHTLQNTYSDLDIHL 257

Query: 499  GQSYSVLVTADQPAQDYYIVVSSRFTDPVLTNTAILHYSNSAGRVSGPPPGGPTTQIDWS 678
            GQSYSVLVTADQPA+DYYIVVSSRFT PVL  TA+LHYSNS  RVSGP PGGPTTQIDWS
Sbjct: 258  GQSYSVLVTADQPAKDYYIVVSSRFTKPVLATTAVLHYSNSWQRVSGPIPGGPTTQIDWS 317

Query: 679  LNQARSIRWNLTASGPRPNPQGSYHYGLINTTRTIRLANSAPVINGKQRYAVNSVSFIPA 858
            LNQARS RWNLTASGPRPNPQGSYHYGLI T+RTI LANS P+IN KQRYAVN VS+IPA
Sbjct: 318  LNQARSFRWNLTASGPRPNPQGSYHYGLIKTSRTITLANSGPIINRKQRYAVNGVSYIPA 377

Query: 859  DTPLKLADYFKISGVFSVGSISDNPT-GGGGYLQTSVMGADFRAFIEIVFENSEDTVQSW 1035
            DTPLK+ADYF I GVFS+GS+  +P+ G   YLQT+VM A+FR FIEIVF+N EDTVQSW
Sbjct: 378  DTPLKIADYFNIPGVFSLGSMPSSPSWGNNAYLQTAVMSANFREFIEIVFQNWEDTVQSW 437

Query: 1036 HVDGYAFFVVGMDGGQWSAASRLNYNLRDTVARSTVQVYPKSWSAIYVGLDNVGMWNIRS 1215
            H+DG++FFVVGMDGGQW+ ASR  YNLRDTVAR T QVYPKSW+AIY+ LDNVGMWNIRS
Sbjct: 438  HIDGHSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTAIYMALDNVGMWNIRS 497

Query: 1216 EDWARQYLGQQFYLRVYSAVESWRDEYPIPKNALLCGRASGRTTRPL 1356
            E+WARQYLGQQFYLRVYS   SWRDE PIPKNALLCGRA GR TRPL
Sbjct: 498  ENWARQYLGQQFYLRVYSPAHSWRDELPIPKNALLCGRARGRHTRPL 544



 Score =  120 bits (302), Expect(2) = 0.0
 Identities = 54/70 (77%), Positives = 58/70 (82%)
 Frame = +3

Query: 3   QIGSFFYFPSLAFHKAAGGFGGITISSRPRIPVPFPSPDGDFTILAGDWFKKNHTDLKAT 182
           QIGSFFYFPSL FHKAAG FGGI I SRPRIPVPFP P GDFT+LAGDW+K+NH  L+  
Sbjct: 121 QIGSFFYFPSLGFHKAAGAFGGIRIWSRPRIPVPFPPPAGDFTVLAGDWYKRNHYQLRRI 180

Query: 183 LDGGSTLPFP 212
           LDGG  LPFP
Sbjct: 181 LDGGHNLPFP 190