BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00011822
         (752 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277900.1| PREDICTED: probable steroid reductase DET2 [...   370   e-100
emb|CAN63063.1| hypothetical protein VITISV_021171 [Vitis vinifera]   370   e-100
ref|XP_002323554.1| predicted protein [Populus trichocarpa] gi|2...   336   3e-90
sp|Q2QDF6.1|DET2_GOSHI RecName: Full=Steroid 5-alpha-reductase D...   325   6e-87
gb|AAN28012.1| steroid 5-alpha-reductase [Gossypium hirsutum]         322   4e-86

>ref|XP_002277900.1| PREDICTED: probable steroid reductase DET2 [Vitis vinifera]
           gi|297740252|emb|CBI30434.3| unnamed protein product
           [Vitis vinifera]
          Length = 257

 Score =  370 bits (950), Expect = e-100
 Identities = 172/242 (71%), Positives = 193/242 (79%)
 Frame = -2

Query: 730 DESLFHYSLLTFFIITPPTFISLRFLQAPYGKHYRKGWGPTISPSLAWFLMESPTIWLTL 551
           D +LFH SL+  ++I PPTFISLRFLQAPYGKH+R GWGPT+ PSLAWFLMESPT+WLTL
Sbjct: 4   DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRSGWGPTVPPSLAWFLMESPTVWLTL 63

Query: 550 LIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRXXXXXXXXXXXXXXKASFPVSVA 371
           LI+P G   SN +S+ LISI+LFHYIHRT+IYPL LR                 FPVSVA
Sbjct: 64  LIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHLRNSTAAKRN--------GFPVSVA 115

Query: 370 LMGFGFNLLNAYLQSRWVSHYYVVENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKLKG 191
           LM FGFNLLNAYLQ+RWVSHY   E+D WF  RF +GLVIFL GM+VNVRSDL LV LK 
Sbjct: 116 LMAFGFNLLNAYLQARWVSHYANYEDDGWFWWRFWVGLVIFLDGMLVNVRSDLALVGLKS 175

Query: 190 EDGGYKIPRGGWFERVSCANYFGEIVEWLGWAVMSWSWVGFAFFIYTCANLVPRARANHI 11
           + GGYK+PRGGWFE VSCANYFGE+VEWLGWAVM+WSWVG  F +YTCANLVPRARANH 
Sbjct: 176 QGGGYKVPRGGWFELVSCANYFGEVVEWLGWAVMTWSWVGLGFLLYTCANLVPRARANHK 235

Query: 10  WY 5
           WY
Sbjct: 236 WY 237


>emb|CAN63063.1| hypothetical protein VITISV_021171 [Vitis vinifera]
          Length = 257

 Score =  370 bits (949), Expect = e-100
 Identities = 172/242 (71%), Positives = 193/242 (79%)
 Frame = -2

Query: 730 DESLFHYSLLTFFIITPPTFISLRFLQAPYGKHYRKGWGPTISPSLAWFLMESPTIWLTL 551
           D +LFH SL+  ++I PPTFISLRFLQAPYGKH+R GWGPT+ PSLAWFLMESPT+WLTL
Sbjct: 4   DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRXGWGPTVPPSLAWFLMESPTVWLTL 63

Query: 550 LIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRXXXXXXXXXXXXXXKASFPVSVA 371
           LI+P G   SN +S+ LISI+LFHYIHRT+IYPL LR                 FPVSVA
Sbjct: 64  LIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHLRNSTAAKRN--------GFPVSVA 115

Query: 370 LMGFGFNLLNAYLQSRWVSHYYVVENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKLKG 191
           LM FGFNLLNAYLQ+RWVSHY   E+D WF  RF +GLVIFL GM+VNVRSDL LV LK 
Sbjct: 116 LMAFGFNLLNAYLQARWVSHYANYEDDGWFWWRFWVGLVIFLDGMLVNVRSDLALVGLKS 175

Query: 190 EDGGYKIPRGGWFERVSCANYFGEIVEWLGWAVMSWSWVGFAFFIYTCANLVPRARANHI 11
           + GGYK+PRGGWFE VSCANYFGE+VEWLGWAVM+WSWVG  F +YTCANLVPRARANH 
Sbjct: 176 QGGGYKVPRGGWFELVSCANYFGEVVEWLGWAVMTWSWVGLGFLLYTCANLVPRARANHK 235

Query: 10  WY 5
           WY
Sbjct: 236 WY 237


>ref|XP_002323554.1| predicted protein [Populus trichocarpa] gi|222868184|gb|EEF05315.1|
           predicted protein [Populus trichocarpa]
          Length = 257

 Score =  336 bits (862), Expect = 3e-90
 Identities = 160/246 (65%), Positives = 187/246 (76%)
 Frame = -2

Query: 742 MKLSDESLFHYSLLTFFIITPPTFISLRFLQAPYGKHYRKGWGPTISPSLAWFLMESPTI 563
           M L D+SLFHYSLL+ ++I PPTFISL+FLQAPYGKH+R GWGPTISP LAW LMESPT+
Sbjct: 1   MALLDQSLFHYSLLSIYLIGPPTFISLKFLQAPYGKHHRPGWGPTISPPLAWILMESPTL 60

Query: 562 WLTLLIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRXXXXXXXXXXXXXXKASFP 383
           WLTLL+FP+GQ+ +N K+I L+S FL HY HRT IYPLR+                  FP
Sbjct: 61  WLTLLLFPYGQHFTNPKAIILMSPFLLHYFHRTCIYPLRI---------YRNSKTTGGFP 111

Query: 382 VSVALMGFGFNLLNAYLQSRWVSHYYVVENDWWFGVRFVIGLVIFLSGMIVNVRSDLILV 203
           VSVA+M FGFNLLNAYLQ+RWVSHY    +   F  +F  GLV+F+ GM +N  +D +L+
Sbjct: 112 VSVAVMAFGFNLLNAYLQARWVSHYKSDYDGGLFWWKFFGGLVVFVWGMRINTWADGVLL 171

Query: 202 KLKGEDGGYKIPRGGWFERVSCANYFGEIVEWLGWAVMSWSWVGFAFFIYTCANLVPRAR 23
            LK E GGYK+PRGGWFE VSC NYFGEIVEWLGWAVM+WSW GF F +YTCANLVPRA 
Sbjct: 172 GLKREGGGYKVPRGGWFELVSCPNYFGEIVEWLGWAVMTWSWAGFGFLLYTCANLVPRAC 231

Query: 22  ANHIWY 5
           ANH WY
Sbjct: 232 ANHKWY 237


>sp|Q2QDF6.1|DET2_GOSHI RecName: Full=Steroid 5-alpha-reductase DET2; Short=GhDET2
           gi|73761693|gb|AAZ83346.1| steroid 5-alpha-reductase
           [Gossypium hirsutum]
          Length = 258

 Score =  325 bits (833), Expect = 6e-87
 Identities = 161/245 (65%), Positives = 184/245 (75%), Gaps = 2/245 (0%)
 Frame = -2

Query: 733 SDESLFHYSLLTFFIITPPTFISLRFLQAPYGKHYRKGWGPTISPSLAWFLMESPTIWLT 554
           SD++LFHY LLT +II  PT+ISL FLQAPYGKH R GWGPTISP LAWFLMESPT+WLT
Sbjct: 3   SDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLT 62

Query: 553 LLIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRXXXXXXXXXXXXXXKASFPVSV 374
             +FP GQ+  N KS  LIS FLFHY +RT++YPLRL                  FPVSV
Sbjct: 63  FFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL---------ARNTTQTRGFPVSV 113

Query: 373 ALMGFGFNLLNAYLQSRWVSHYY-VVENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKL 197
           A M FGFNLLN YLQ+RWVSHY    EN+  F  RF+ GL+IF+ GM VNVR+D +LV L
Sbjct: 114 AFMAFGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGL 173

Query: 196 KGE-DGGYKIPRGGWFERVSCANYFGEIVEWLGWAVMSWSWVGFAFFIYTCANLVPRARA 20
           K + DGGYKIPRGG FE VSC NYFGEI+EW GWAVM+WSWVGF FF+YTCANL+PRARA
Sbjct: 174 KKQGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARA 233

Query: 19  NHIWY 5
             +WY
Sbjct: 234 TRLWY 238


>gb|AAN28012.1| steroid 5-alpha-reductase [Gossypium hirsutum]
          Length = 258

 Score =  322 bits (826), Expect = 4e-86
 Identities = 160/247 (64%), Positives = 184/247 (74%), Gaps = 4/247 (1%)
 Frame = -2

Query: 733 SDESLFHYSLLTFFIITPPTFISLRFLQAPYGKHYRKGWGPTISPSLAWFLMESPTIWLT 554
           SD++LFHY LLT +II  PT+ISL FLQAPYGKH R GWGPTISP LAWFLMESPT+WLT
Sbjct: 3   SDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLT 62

Query: 553 LLIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRXXXXXXXXXXXXXXKASFPVSV 374
             +FP GQ+  N KS  LIS FLFHY +RT++YPLRL                  FPVSV
Sbjct: 63  FFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL---------ARNTTQTRGFPVSV 113

Query: 373 ALMGFGFNLLNAYLQSRWVSHY---YVVENDWWFGVRFVIGLVIFLSGMIVNVRSDLILV 203
           A M FGFNLLN YLQ+RWVSHY   Y  E  +W+  RF+ GL+IF+ GM VNV +D +LV
Sbjct: 114 AFMAFGFNLLNGYLQARWVSHYKDXYXNEELFWW--RFLXGLLIFVVGMWVNVXADKVLV 171

Query: 202 KLKGE-DGGYKIPRGGWFERVSCANYFGEIVEWLGWAVMSWSWVGFAFFIYTCANLVPRA 26
            LK + DGGYKIPRGG FE VSC NYFGEI+EW GWAVM+WSWVGF FF+YTCANL+PRA
Sbjct: 172 GLKKQGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRA 231

Query: 25  RANHIWY 5
           RA  +WY
Sbjct: 232 RATRLWY 238