BLASTX nr result
ID: Coptis23_contig00010840
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00010840
(2207 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002278450.2| PREDICTED: subtilisin-like protease [Vitis v... 332 e-131
emb|CBI34615.3| unnamed protein product [Vitis vinifera] 332 e-131
ref|XP_003524182.1| PREDICTED: subtilisin-like protease SDD1-lik... 306 e-121
ref|XP_003629621.1| Subtilisin-like protease [Medicago truncatul... 300 e-121
ref|XP_002317660.1| predicted protein [Populus trichocarpa] gi|2... 309 e-118
>ref|XP_002278450.2| PREDICTED: subtilisin-like protease [Vitis vinifera]
Length = 787
Score = 332 bits (851), Expect(4) = e-131
Identities = 161/225 (71%), Positives = 179/225 (79%)
Frame = +1
Query: 1003 TGIWPELESFHDKNMEEIPSRWKGICQHGEHFNSTNCNKKLIGARWFIKGIRHGTNKNIN 1182
+G+WPE ESF D+ M IPSRWKGICQHGE FNSTNCN+KLIGARWF KGI K +N
Sbjct: 160 SGVWPESESFKDEGMGPIPSRWKGICQHGERFNSTNCNRKLIGARWFFKGIHQEIGKFMN 219
Query: 1183 TTGTREFLSPRDGIGHGTHTASTSAGRLVKKASYXXXXXXXXXXXXPSARLAIYKACWAI 1362
T EFLSPRDGIGHGTHTAST+AG V+KA+Y P ARLAIYKACWAI
Sbjct: 220 ITDNLEFLSPRDGIGHGTHTASTAAGYFVEKANYRGLATGLARGGAPLARLAIYKACWAI 279
Query: 1363 EDEGCTDADILKAFDKAIHDGVDILSLSLGIQIPLFPFTSQYDAISIGSFHATAKGITVV 1542
C+DADILKAFDKAIHDGVDILSLS+G IPLF + Q D+I+I SFHA AKGITVV
Sbjct: 280 ISGACSDADILKAFDKAIHDGVDILSLSVGNDIPLFSYVDQRDSIAIASFHAIAKGITVV 339
Query: 1543 CSAGNDGPFSQTMVNTAPWLITVAATTIDRAFPTSILLGSNRTLL 1677
CSAGNDGPFSQT+ NTAPWLITVAATTIDRAFPT+I+LG+N+T L
Sbjct: 340 CSAGNDGPFSQTIANTAPWLITVAATTIDRAFPTAIILGNNQTFL 384
Score = 92.8 bits (229), Expect(4) = e-131
Identities = 45/76 (59%), Positives = 60/76 (78%)
Frame = +1
Query: 1960 SKGCKYGSLDAKLATGKVVLCFSVADVPDMVSASLAVQEAGGSGLIYAQSRDSLLSSCNF 2139
+K C+ GSL+A LA GK++LCFS +D D++SAS AV EAGG GLI+AQ S L SC+
Sbjct: 415 AKDCQPGSLNATLAAGKIILCFSKSDKQDIISASGAVLEAGGIGLIFAQFPTSQLESCDL 474
Query: 2140 IPCIQVDYEVGTKLLS 2187
IPCI+V+YEVGT++L+
Sbjct: 475 IPCIKVNYEVGTQILT 490
Score = 76.3 bits (186), Expect(4) = e-131
Identities = 33/52 (63%), Positives = 43/52 (82%)
Frame = +2
Query: 764 YSGVMKVIPNRIHKLHTTRSWDFLGLSSHHPRDLFSESNMGAETIIGVIDSG 919
+ GV++VIPNRIH+LHTTRSWDFLGL +P ++ +E+N+G IIGVIDSG
Sbjct: 110 FPGVVQVIPNRIHRLHTTRSWDFLGLQHDYPTNVLTETNLGRGVIIGVIDSG 161
Score = 40.8 bits (94), Expect(4) = e-131
Identities = 17/33 (51%), Positives = 23/33 (69%)
Frame = +2
Query: 1757 GQSMGSREHEEGFNGLVYSEHIAIDPSDDER*D 1855
GQS+ + +H+ GF GL YSE +A+DP DD D
Sbjct: 385 GQSIDTGKHKLGFTGLTYSERVALDPKDDSAKD 417
>emb|CBI34615.3| unnamed protein product [Vitis vinifera]
Length = 737
Score = 332 bits (851), Expect(4) = e-131
Identities = 161/225 (71%), Positives = 179/225 (79%)
Frame = +1
Query: 1003 TGIWPELESFHDKNMEEIPSRWKGICQHGEHFNSTNCNKKLIGARWFIKGIRHGTNKNIN 1182
+G+WPE ESF D+ M IPSRWKGICQHGE FNSTNCN+KLIGARWF KGI K +N
Sbjct: 110 SGVWPESESFKDEGMGPIPSRWKGICQHGERFNSTNCNRKLIGARWFFKGIHQEIGKFMN 169
Query: 1183 TTGTREFLSPRDGIGHGTHTASTSAGRLVKKASYXXXXXXXXXXXXPSARLAIYKACWAI 1362
T EFLSPRDGIGHGTHTAST+AG V+KA+Y P ARLAIYKACWAI
Sbjct: 170 ITDNLEFLSPRDGIGHGTHTASTAAGYFVEKANYRGLATGLARGGAPLARLAIYKACWAI 229
Query: 1363 EDEGCTDADILKAFDKAIHDGVDILSLSLGIQIPLFPFTSQYDAISIGSFHATAKGITVV 1542
C+DADILKAFDKAIHDGVDILSLS+G IPLF + Q D+I+I SFHA AKGITVV
Sbjct: 230 ISGACSDADILKAFDKAIHDGVDILSLSVGNDIPLFSYVDQRDSIAIASFHAIAKGITVV 289
Query: 1543 CSAGNDGPFSQTMVNTAPWLITVAATTIDRAFPTSILLGSNRTLL 1677
CSAGNDGPFSQT+ NTAPWLITVAATTIDRAFPT+I+LG+N+T L
Sbjct: 290 CSAGNDGPFSQTIANTAPWLITVAATTIDRAFPTAIILGNNQTFL 334
Score = 92.8 bits (229), Expect(4) = e-131
Identities = 45/76 (59%), Positives = 60/76 (78%)
Frame = +1
Query: 1960 SKGCKYGSLDAKLATGKVVLCFSVADVPDMVSASLAVQEAGGSGLIYAQSRDSLLSSCNF 2139
+K C+ GSL+A LA GK++LCFS +D D++SAS AV EAGG GLI+AQ S L SC+
Sbjct: 365 AKDCQPGSLNATLAAGKIILCFSKSDKQDIISASGAVLEAGGIGLIFAQFPTSQLESCDL 424
Query: 2140 IPCIQVDYEVGTKLLS 2187
IPCI+V+YEVGT++L+
Sbjct: 425 IPCIKVNYEVGTQILT 440
Score = 76.3 bits (186), Expect(4) = e-131
Identities = 33/52 (63%), Positives = 43/52 (82%)
Frame = +2
Query: 764 YSGVMKVIPNRIHKLHTTRSWDFLGLSSHHPRDLFSESNMGAETIIGVIDSG 919
+ GV++VIPNRIH+LHTTRSWDFLGL +P ++ +E+N+G IIGVIDSG
Sbjct: 60 FPGVVQVIPNRIHRLHTTRSWDFLGLQHDYPTNVLTETNLGRGVIIGVIDSG 111
Score = 40.8 bits (94), Expect(4) = e-131
Identities = 17/33 (51%), Positives = 23/33 (69%)
Frame = +2
Query: 1757 GQSMGSREHEEGFNGLVYSEHIAIDPSDDER*D 1855
GQS+ + +H+ GF GL YSE +A+DP DD D
Sbjct: 335 GQSIDTGKHKLGFTGLTYSERVALDPKDDSAKD 367
>ref|XP_003524182.1| PREDICTED: subtilisin-like protease SDD1-like [Glycine max]
Length = 774
Score = 306 bits (785), Expect(4) = e-121
Identities = 147/224 (65%), Positives = 171/224 (76%)
Frame = +1
Query: 1003 TGIWPELESFHDKNMEEIPSRWKGICQHGEHFNSTNCNKKLIGARWFIKGIRHGTNKNIN 1182
TGIWPE SF+D+ M +IPSRWKGICQ G+HFNSTNCNKK+IGARWF+KGI T K +
Sbjct: 152 TGIWPESPSFNDEAMGQIPSRWKGICQGGKHFNSTNCNKKIIGARWFMKGISDQTKKLLQ 211
Query: 1183 TTGTREFLSPRDGIGHGTHTASTSAGRLVKKASYXXXXXXXXXXXXPSARLAIYKACWAI 1362
+ E+LS RD IGHGTHTAST+AG V A+Y P A LAIYKACW
Sbjct: 212 GNNSDEYLSARDAIGHGTHTASTAAGYFVGNANYRGLASGLARGGAPLAHLAIYKACWDF 271
Query: 1363 EDEGCTDADILKAFDKAIHDGVDILSLSLGIQIPLFPFTSQYDAISIGSFHATAKGITVV 1542
CTDADILKAFDKAIHDGVD+L++SLG IPLF + Q D+++IGSFHAT+KGITVV
Sbjct: 272 PIGDCTDADILKAFDKAIHDGVDVLTVSLGFAIPLFSYVDQRDSLAIGSFHATSKGITVV 331
Query: 1543 CSAGNDGPFSQTMVNTAPWLITVAATTIDRAFPTSILLGSNRTL 1674
CSAGN GP SQT+ NTAPW+ITV ATTIDRAFP +I LG+NRT+
Sbjct: 332 CSAGNSGPVSQTVTNTAPWIITVGATTIDRAFPAAITLGNNRTV 375
Score = 95.9 bits (237), Expect(4) = e-121
Identities = 45/76 (59%), Positives = 59/76 (77%)
Frame = +1
Query: 1960 SKGCKYGSLDAKLATGKVVLCFSVADVPDMVSASLAVQEAGGSGLIYAQSRDSLLSSCNF 2139
+K C+ GSL+A +A GK+VLCFSV+D D+VSASL V+EAGG GL+YAQ + L+ C
Sbjct: 407 AKDCQSGSLNATMAAGKIVLCFSVSDQQDIVSASLTVKEAGGVGLVYAQYHEDGLNQCGS 466
Query: 2140 IPCIQVDYEVGTKLLS 2187
PCI+VDYEVGT+ L+
Sbjct: 467 FPCIKVDYEVGTQTLT 482
Score = 70.5 bits (171), Expect(4) = e-121
Identities = 32/52 (61%), Positives = 40/52 (76%)
Frame = +2
Query: 764 YSGVMKVIPNRIHKLHTTRSWDFLGLSSHHPRDLFSESNMGAETIIGVIDSG 919
+ GV+ VIPN IHKLHTTRSWDF+G+ + FS+SN+G TIIGVID+G
Sbjct: 102 FPGVVSVIPNGIHKLHTTRSWDFMGVHHSTSKIAFSDSNLGEGTIIGVIDTG 153
Score = 35.4 bits (80), Expect(4) = e-121
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2
Query: 1757 GQSMGSREHEEGFNGLVYSEHIAIDPSDD 1843
GQS+ +H G GL YSE IA+DPSD+
Sbjct: 377 GQSIDMGKHNLGSVGLTYSERIAVDPSDN 405
>ref|XP_003629621.1| Subtilisin-like protease [Medicago truncatula]
gi|355523643|gb|AET04097.1| Subtilisin-like protease
[Medicago truncatula]
Length = 797
Score = 300 bits (768), Expect(4) = e-121
Identities = 149/227 (65%), Positives = 171/227 (75%), Gaps = 1/227 (0%)
Frame = +1
Query: 1003 TGIWPELESFHDKNMEEIPSRWKGICQHGEHFNSTNCNKKLIGARWFIKGIR-HGTNKNI 1179
TGIWPE SF+D+ M +IPS+WKG+CQ GE FNSTNCNKK+IGARWF+KGI H N +
Sbjct: 156 TGIWPESASFNDEAMGKIPSKWKGVCQVGEKFNSTNCNKKIIGARWFLKGITDHTKNLVL 215
Query: 1180 NTTGTREFLSPRDGIGHGTHTASTSAGRLVKKASYXXXXXXXXXXXXPSARLAIYKACWA 1359
T E+LS RD IGHGTHTAST+AG V+ A+Y P A LAIYKACW
Sbjct: 216 GNNDTTEYLSARDAIGHGTHTASTAAGYFVENANYRGLASGLARGGAPLAHLAIYKACWD 275
Query: 1360 IEDEGCTDADILKAFDKAIHDGVDILSLSLGIQIPLFPFTSQYDAISIGSFHATAKGITV 1539
+ CTDADILKAFD AIHDGVD+L++SLGI IPLF + Q D I+IGSFHAT+KGITV
Sbjct: 276 VPVGHCTDADILKAFDMAIHDGVDVLTVSLGIGIPLFSYADQRDTIAIGSFHATSKGITV 335
Query: 1540 VCSAGNDGPFSQTMVNTAPWLITVAATTIDRAFPTSILLGSNRTLLV 1680
V SAGN GP SQT+ NTAPWLITVAATTIDR FPT+I LG+N TL V
Sbjct: 336 VSSAGNSGPISQTVSNTAPWLITVAATTIDRTFPTAITLGNNLTLWV 382
Score = 95.9 bits (237), Expect(4) = e-121
Identities = 44/76 (57%), Positives = 60/76 (78%)
Frame = +1
Query: 1960 SKGCKYGSLDAKLATGKVVLCFSVADVPDMVSASLAVQEAGGSGLIYAQSRDSLLSSCNF 2139
+K C+ GSL+ +A GK+VLCFSV+D D+VSA+L+V+EAGG GLIYAQ + L+ C
Sbjct: 422 AKDCQSGSLNETMAAGKIVLCFSVSDQQDIVSAALSVKEAGGVGLIYAQRHEDGLNECGI 481
Query: 2140 IPCIQVDYEVGTKLLS 2187
+PCI+VDYE GT+LL+
Sbjct: 482 LPCIKVDYEAGTELLT 497
Score = 70.1 bits (170), Expect(4) = e-121
Identities = 34/51 (66%), Positives = 42/51 (82%), Gaps = 2/51 (3%)
Frame = +2
Query: 773 VMKVIPNRIHKLHTTRSWDFLGLSSHHP--RDLFSESNMGAETIIGVIDSG 919
V+ VIPN IHKLHTTRSWDF+G+ HHP + +F+ESN+G TIIGVID+G
Sbjct: 109 VVSVIPNGIHKLHTTRSWDFIGV--HHPSSKTVFTESNLGQGTIIGVIDTG 157
Score = 40.8 bits (94), Expect(4) = e-121
Identities = 19/33 (57%), Positives = 23/33 (69%)
Frame = +2
Query: 1745 CLLQGQSMGSREHEEGFNGLVYSEHIAIDPSDD 1843
C+ GQS+ + +H GF GL YSE IA DPSDD
Sbjct: 388 CIELGQSIDNGKHALGFVGLTYSERIARDPSDD 420
>ref|XP_002317660.1| predicted protein [Populus trichocarpa] gi|222860725|gb|EEE98272.1|
predicted protein [Populus trichocarpa]
Length = 786
Score = 309 bits (792), Expect(4) = e-118
Identities = 145/224 (64%), Positives = 174/224 (77%)
Frame = +1
Query: 1003 TGIWPELESFHDKNMEEIPSRWKGICQHGEHFNSTNCNKKLIGARWFIKGIRHGTNKNIN 1182
+G+WPE +SFHD+ M +PS WKGICQ GE FNS+NCN+K+IGARWF+KG + N
Sbjct: 164 SGVWPESKSFHDEGMGPVPSHWKGICQQGESFNSSNCNRKIIGARWFVKGFQD--QLPFN 221
Query: 1183 TTGTREFLSPRDGIGHGTHTASTSAGRLVKKASYXXXXXXXXXXXXPSARLAIYKACWAI 1362
TT +REF+SPRDG GHG+HTAST+AG V+K SY P A LAIYK CW I
Sbjct: 222 TTESREFMSPRDGEGHGSHTASTAAGNFVEKVSYKGLAAGLARGGAPLAHLAIYKVCWNI 281
Query: 1363 EDEGCTDADILKAFDKAIHDGVDILSLSLGIQIPLFPFTSQYDAISIGSFHATAKGITVV 1542
ED GCTDAD+LKAFDKAIHDGVDILS+S+G IPLF + ++I+IGSFHAT GI+V+
Sbjct: 282 EDGGCTDADLLKAFDKAIHDGVDILSVSIGNNIPLFSYVDMRNSIAIGSFHATLNGISVI 341
Query: 1543 CSAGNDGPFSQTMVNTAPWLITVAATTIDRAFPTSILLGSNRTL 1674
CSAGNDGP SQT+ NTAPWLITVAA+TIDR FPT+I LG+N+TL
Sbjct: 342 CSAGNDGPISQTVENTAPWLITVAASTIDRTFPTAITLGNNKTL 385
Score = 79.3 bits (194), Expect(4) = e-118
Identities = 35/52 (67%), Positives = 44/52 (84%)
Frame = +2
Query: 764 YSGVMKVIPNRIHKLHTTRSWDFLGLSSHHPRDLFSESNMGAETIIGVIDSG 919
+ GV++VIPN IHKLHTTRSW+F+GL+ H P++L +SNMG TIIGVIDSG
Sbjct: 114 FPGVVQVIPNGIHKLHTTRSWEFIGLNHHSPQNLLRQSNMGQGTIIGVIDSG 165
Score = 78.2 bits (191), Expect(4) = e-118
Identities = 39/76 (51%), Positives = 54/76 (71%)
Frame = +1
Query: 1960 SKGCKYGSLDAKLATGKVVLCFSVADVPDMVSASLAVQEAGGSGLIYAQSRDSLLSSCNF 2139
+K C+ GSL+A LA GK++LC S ++ DM SAS +V EAGG GLI+ Q + C
Sbjct: 417 AKDCQPGSLNATLAAGKIILCLSESNTQDMFSASTSVFEAGGVGLIFVQFHLDGMELCK- 475
Query: 2140 IPCIQVDYEVGTKLLS 2187
IPC++VDYEVGT+++S
Sbjct: 476 IPCVKVDYEVGTQIVS 491
Score = 32.0 bits (71), Expect(4) = e-118
Identities = 15/35 (42%), Positives = 20/35 (57%)
Frame = +2
Query: 1751 LQGQSMGSREHEEGFNGLVYSEHIAIDPSDDER*D 1855
L GQS+ + +H GF L YSE I ++P D D
Sbjct: 385 LWGQSITTGQHNHGFASLTYSERIPLNPMVDSAKD 419