BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00010767
         (886 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      486   e-135
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   425   e-117
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       379   e-103
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   318   1e-84
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   317   3e-84

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  486 bits (1250), Expect = e-135
 Identities = 242/260 (93%), Positives = 244/260 (93%)
 Frame = -2

Query: 780 MTVEAKQPKKAAIXXXXXXXXXXXVPYDDIKHLIRSELARRLQWGYKPTYEEQTAEIQNL 601
           M VEAKQ KKAAI           VPYDDIK LIR ELARRLQWGYKPTYEEQ AEIQNL
Sbjct: 1   MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNL 60

Query: 600 THSLRQMKIATEVDTLDSQMYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 421
           THSLRQMKIATEV+TLDSQ+YEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL
Sbjct: 61  THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 120

Query: 420 YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 241
           YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN
Sbjct: 121 YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 180

Query: 240 VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 61
           VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA
Sbjct: 181 VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 240

Query: 60  YHYEPLDDDDWFTEYVFPAG 1
           YHYEPLDDDDWFTEYVFPAG
Sbjct: 241 YHYEPLDDDDWFTEYVFPAG 260


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  425 bits (1093), Expect = e-117
 Identities = 210/262 (80%), Positives = 230/262 (87%), Gaps = 2/262 (0%)
 Frame = -2

Query: 780 MTVEAKQ--PKKAAIXXXXXXXXXXXVPYDDIKHLIRSELARRLQWGYKPTYEEQTAEIQ 607
           M VE KQ  PKKA I           VP D+IK LIR +L RRLQWG K TYEEQ A++ 
Sbjct: 1   MAVEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLV 60

Query: 606 NLTHSLRQMKIATEVDTLDSQMYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAML 427
           NLTHSLRQMKIATEV+TLD QMYE+PI+FLKIMNGSNLKGSCCYFK DSTTLDEAEIAML
Sbjct: 61  NLTHSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAML 120

Query: 426 DLYCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNL 247
           +LYCERAQI+DG SVLDLGCGQGALTL+VAQKYKN RVTAVTNSVSQKE+IEEESR+RNL
Sbjct: 121 ELYCERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNL 180

Query: 246 LNVEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKT 67
            NVEV LADITTH+M +TYDRILV+ELFEHMKNYELLLRKI EW++KDGLLF+EHICHKT
Sbjct: 181 SNVEVLLADITTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKT 240

Query: 66  FAYHYEPLDDDDWFTEYVFPAG 1
           FAYHYEP+D+DDWFTEYVFPAG
Sbjct: 241 FAYHYEPIDEDDWFTEYVFPAG 262


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  379 bits (974), Expect = e-103
 Identities = 179/235 (76%), Positives = 208/235 (88%)
 Frame = -2

Query: 705 PYDDIKHLIRSELARRLQWGYKPTYEEQTAEIQNLTHSLRQMKIATEVDTLDSQMYEIPI 526
           P D++K L+R +LARRLQWGYK TYEEQ A++ +LTHSLR M IA EVDTLDS MYE+P+
Sbjct: 19  PNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVDTLDSNMYEVPL 78

Query: 525 EFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTL 346
           +FLKIMNGS LK S CYFK+DSTTLDEAEIAM+DL+CERAQI+DG SVLDLGCG G+LT 
Sbjct: 79  DFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVLDLGCGHGSLTF 138

Query: 345 HVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIEL 166
           +VAQKYK   VTAVTNSVSQKEYIEEE+RRRNL NVEV LADI THEMA+TYDRILV+ L
Sbjct: 139 YVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMADTYDRILVVGL 198

Query: 165 FEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAG 1
           FEHMKNY LLL+KISEW++KDGLLF++H+CHKTFA+H EP+D+DDW +EY FPAG
Sbjct: 199 FEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSEYAFPAG 253


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
           somniferum]
          Length = 351

 Score =  318 bits (814), Expect = 1e-84
 Identities = 148/235 (62%), Positives = 189/235 (80%)
 Frame = -2

Query: 705 PYDDIKHLIRSELARRLQWGYKPTYEEQTAEIQNLTHSLRQMKIATEVDTLDSQMYEIPI 526
           P  +I+ LIR EL +RLQWGYK T+EEQ +++ +L HSL+ MK+ATE++ LD ++YE P+
Sbjct: 19  PDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENLDLKLYEAPM 78

Query: 525 EFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTL 346
           EFLKI +GSN+K S  Y+ ++STTLDEAEIAMLDLY ERAQI+DGQSVLDLGCG GA+ L
Sbjct: 79  EFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDLGCGLGAVAL 138

Query: 345 HVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIEL 166
             A K+K C+ T VT+SV QK+YIE + +   L NV+V LADITT+E  E +DRI  +EL
Sbjct: 139 FGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETEERFDRIFAVEL 198

Query: 165 FEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAG 1
            EHMKNY+LLL+KISEW+  DGLLF+EH+CHKT AYHYEP+D +DW+T Y+FPAG
Sbjct: 199 IEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYIFPAG 253


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
           gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
           [Thalictrum flavum]
          Length = 356

 Score =  317 bits (811), Expect = 3e-84
 Identities = 146/258 (56%), Positives = 198/258 (76%)
 Frame = -2

Query: 774 VEAKQPKKAAIXXXXXXXXXXXVPYDDIKHLIRSELARRLQWGYKPTYEEQTAEIQNLTH 595
           +E KQ KK A+           V  ++I+ +++ ++ +RL+WGYKPT+E+Q A++     
Sbjct: 1   METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60

Query: 594 SLRQMKIATEVDTLDSQMYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYC 415
           SL+ M++A EVDTLD+++YEIP+ FL IM G  LK S  YFK++STTLDE+E+ M+DLYC
Sbjct: 61  SLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYC 120

Query: 414 ERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVE 235
           ERAQI+DGQS+LDLGCG G+LTLHVAQKY+ C+VT +TNSVSQKE+I ++ ++ +L NVE
Sbjct: 121 ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVE 180

Query: 234 VKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYH 55
           + L D+T  E   TYDRI  + L EHMKNYEL L+K+S WI++ GLLF+EH CHK FAY 
Sbjct: 181 IILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQ 240

Query: 54  YEPLDDDDWFTEYVFPAG 1
           YEPLD+DDW+TEY+FP+G
Sbjct: 241 YEPLDEDDWYTEYIFPSG 258