BLASTX nr result
ID: Coptis23_contig00010308
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00010308
(2123 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002278135.1| PREDICTED: putative disease resistance prote... 567 e-159
emb|CAN62186.1| hypothetical protein VITISV_003708 [Vitis vinifera] 564 e-158
ref|XP_002511340.1| Disease resistance protein RPP13, putative [... 490 e-136
ref|XP_003633825.1| PREDICTED: disease resistance protein RPM1-l... 416 e-113
ref|XP_002318063.1| predicted protein [Populus trichocarpa] gi|2... 411 e-112
>ref|XP_002278135.1| PREDICTED: putative disease resistance protein At1g58400-like [Vitis
vinifera]
Length = 653
Score = 567 bits (1461), Expect = e-159
Identities = 318/648 (49%), Positives = 415/648 (64%), Gaps = 5/648 (0%)
Frame = +3
Query: 3 LQRLKSVLLEENQKISSLEDQTQILISLLDEIGGFFESTDGTTGKNCELETRWFRELIDI 182
+QRL S+L++E + S ED+ ++ +L EIG F S + + + + W EL I
Sbjct: 10 IQRLDSLLVQEAESFSGFEDEVLRIMGILREIGSF--SAESGSAEEIDAVHSWVHELKGI 67
Query: 183 VYSMEDCIDNFVVHMENQSASDQTILIDQCKIELENLEKSLLHVRNQMLEDKKGKAVKPE 362
+ ++D +D F++ M+ + SDQ + ID + EL+ L ++M E ++K E
Sbjct: 68 INELDDSVDEFIIQMDAHNGSDQKVWIDSFRSELQKAGSRLAETVHRMRE--LNNSIKGE 125
Query: 363 SLDVEENTSKADAEPGEASSSPTSFKVYLSNLPYFLQSCLKYCCIFPENYAIPKGRLVRL 542
+EN D GE S+S ++ K+ +NLPY LQ CL YCCIFPENY I KGRL+RL
Sbjct: 126 K-GKDENFHGMDE--GETSAS-SALKLQYTNLPYHLQICLTYCCIFPENYWINKGRLIRL 181
Query: 543 LVAEGLIQEKPGQIAEDIAKENIKELANLGVLHV-----DGNKLKVPETIRAFMLCMMGE 707
+AEGLIQ K G++ EDIA+ENI EL NLG+L V +G KL VP R L + E
Sbjct: 182 FIAEGLIQGKAGEVIEDIAQENINELINLGMLQVVDHPGNGIKLVVPSHYREVCLHKIKE 241
Query: 708 EGFIVSCTSSDINVPPTTRRLSIHXXXXXXXXXXXXXXXRSLFLFGIRSLSDAALDSLRP 887
E I +C SSD + P T R +SIH RSLFLFG + S+ D L
Sbjct: 242 EKLITACPSSDSSFPLTVRCVSIHSDMKQVETVLNNVRTRSLFLFGKKGSSEENWDWLNR 301
Query: 888 VFHEKKFLRVLDLEDVHIKNIPDELGDMIHLRYLGLKNSDIDALPESVTNLRNLQTLDIR 1067
+ KFLRVLDLED IK++PDE+GD++HLRYLGLK + I+ LPE + NLR LQTLDIR
Sbjct: 302 --YGSKFLRVLDLEDSKIKHLPDEVGDLMHLRYLGLKKTHINELPERLGNLRALQTLDIR 359
Query: 1068 WNGKITDISSSILKLVQLRHLKMFKNWGINGVREILVPTDIRRSENLQTFTXXXXXXXXX 1247
W G +T++ +L L++LRHLKMFK+ GI G+ VP I R ++L T T
Sbjct: 360 WCGNLTEVPKGVLNLLRLRHLKMFKSIGIGGMD---VPKGIGRIKSLLTLTGIYAGDGIA 416
Query: 1248 XXXXXXTQLKRLGVMDVAADHVSDLSVSIMKMKGLHSLSLEAKSSYXXXXXXXXXXXXXX 1427
QL+RLGVMDVA D VS+L VS+MKM+ L LSL+AK ++
Sbjct: 417 RELGSLIQLRRLGVMDVAEDDVSELFVSVMKMQELLCLSLQAKRTFHQGTLILLETFVPP 476
Query: 1428 XALQKLRLEGRLEKMPSWFSLMENLIKLRLGFSHLSEDPALVLQFLPNLKHLTLWRAYDG 1607
L+KL+LEG LE++P+W LME L LRLG+SHLSE+P VLQ LPNLK LTLW+A++
Sbjct: 477 PLLRKLQLEGVLERIPNWLGLMERLTYLRLGYSHLSENPTSVLQLLPNLKILTLWQAFEA 536
Query: 1608 KQIGKEFCGVGRFPKLEVLVIASHVLEEWTELEEGALPNLKYLHMHNCLRLRMLPEGLQH 1787
K IGKEFC G FPKLE L IASHVLEEWTELEEGA+P+LK+L + NCLRLR LPEGLQ
Sbjct: 537 KHIGKEFCRAGGFPKLEDLTIASHVLEEWTELEEGAIPSLKFLRLRNCLRLRTLPEGLQF 596
Query: 1788 IATLQELKLIPLLDDHLERLKPDGGEENHKIRNIPLISFMPTSVVEQL 1931
I TL++L L+PLLDDH ERL+PDGGEEN+KI++IP + F+ S V Q+
Sbjct: 597 ITTLKQLSLLPLLDDHEERLRPDGGEENYKIKHIPQVKFITMSAVNQI 644
>emb|CAN62186.1| hypothetical protein VITISV_003708 [Vitis vinifera]
Length = 653
Score = 564 bits (1453), Expect = e-158
Identities = 318/648 (49%), Positives = 415/648 (64%), Gaps = 5/648 (0%)
Frame = +3
Query: 3 LQRLKSVLLEENQKISSLEDQTQILISLLDEIGGFFESTDGTTGKNCELETRWFRELIDI 182
+QRL S+L++E + S ED+ ++ +L EIG F S + + + + W EL I
Sbjct: 10 IQRLDSLLVQEAESFSGFEDEVLRIMGILREIGSF--SAESGSAEEIDAVHSWVHELKGI 67
Query: 183 VYSMEDCIDNFVVHMENQSASDQTILIDQCKIELENLEKSLLHVRNQMLEDKKGKAVKPE 362
+ ++D +D F++ M+ + SDQ + ID + EL+ + ++M E ++K E
Sbjct: 68 INELDDSVDEFIIQMDAHNGSDQKVWIDSFRSELQKAGSRVAETVHRMRE--LNNSIKGE 125
Query: 363 SLDVEENTSKADAEPGEASSSPTSFKVYLSNLPYFLQSCLKYCCIFPENYAIPKGRLVRL 542
+ +EN D GE S+S ++ K+ +NLPY LQ CL YCCIFPENY I KGRL+RL
Sbjct: 126 KGE-DENFHGMDE--GETSAS-SALKLQYTNLPYHLQICLTYCCIFPENYWINKGRLIRL 181
Query: 543 LVAEGLIQEKPGQIAEDIAKENIKELANLGVLHV-----DGNKLKVPETIRAFMLCMMGE 707
+AEGLIQ K G++ EDIA+ENI EL NLG+L V +G KL VP R L + E
Sbjct: 182 FIAEGLIQGKAGEVIEDIAQENINELINLGMLQVVDHPGNGIKLVVPSHYREVCLHKIKE 241
Query: 708 EGFIVSCTSSDINVPPTTRRLSIHXXXXXXXXXXXXXXXRSLFLFGIRSLSDAALDSLRP 887
E I +C SSD + P T R +SIH RSLFLFG + S+ D L
Sbjct: 242 EKLITACPSSDSSFPLTVRCVSIHSDMKQVETVLNNVRTRSLFLFGKKGSSEENWDWLNR 301
Query: 888 VFHEKKFLRVLDLEDVHIKNIPDELGDMIHLRYLGLKNSDIDALPESVTNLRNLQTLDIR 1067
+ KFLRVLDLED IK++PDE+GD++HLRYLGLK + I+ LPE + NLR LQTLDIR
Sbjct: 302 --YGSKFLRVLDLEDSKIKHLPDEVGDLMHLRYLGLKKTHINELPERLGNLRALQTLDIR 359
Query: 1068 WNGKITDISSSILKLVQLRHLKMFKNWGINGVREILVPTDIRRSENLQTFTXXXXXXXXX 1247
W G +T + +L L++LRHLKMFK+ GI G+ VP I R ++L T T
Sbjct: 360 WCGNLTXVPKGVLNLLRLRHLKMFKSIGIGGMD---VPKGIGRIKSLLTLTGIYAGDGIA 416
Query: 1248 XXXXXXTQLKRLGVMDVAADHVSDLSVSIMKMKGLHSLSLEAKSSYXXXXXXXXXXXXXX 1427
QL+RLGVMDVA D VS+L VS+MKM+ L LSL+AK +
Sbjct: 417 RELGSLIQLRRLGVMDVAEDDVSELFVSVMKMQELLCLSLQAKXXFHQGTLXLLETFVPP 476
Query: 1428 XALQKLRLEGRLEKMPSWFSLMENLIKLRLGFSHLSEDPALVLQFLPNLKHLTLWRAYDG 1607
L+KL+LEG LE++P+W LME L LRLG+SHLSE+P VLQ LPNLK LTLW+A++
Sbjct: 477 PLLRKLQLEGVLERIPNWLGLMERLTYLRLGYSHLSENPTSVLQLLPNLKILTLWQAFEA 536
Query: 1608 KQIGKEFCGVGRFPKLEVLVIASHVLEEWTELEEGALPNLKYLHMHNCLRLRMLPEGLQH 1787
K IGKEFC G FPKLE L IASHVLEEWTELEEGA+P+LK+L + NCLRLR LPEGLQ
Sbjct: 537 KHIGKEFCRAGGFPKLEDLTIASHVLEEWTELEEGAIPSLKFLXLRNCLRLRTLPEGLQF 596
Query: 1788 IATLQELKLIPLLDDHLERLKPDGGEENHKIRNIPLISFMPTSVVEQL 1931
I TL++L L+PLLDDH ERL+PDGGEEN+KI++IP + F+ S V Q+
Sbjct: 597 ITTLKQLSLLPLLDDHEERLRPDGGEENYKIKHIPQVKFITMSAVNQI 644
>ref|XP_002511340.1| Disease resistance protein RPP13, putative [Ricinus communis]
gi|223550455|gb|EEF51942.1| Disease resistance protein
RPP13, putative [Ricinus communis]
Length = 685
Score = 490 bits (1262), Expect = e-136
Identities = 310/690 (44%), Positives = 394/690 (57%), Gaps = 39/690 (5%)
Frame = +3
Query: 3 LQRLKSVLLEENQK--ISSLEDQTQILISLLDEIGGFFESTDGTTGKNCELE-TRWFREL 173
LQRL S +E Q + L+DQ + + LL EI FF G G + + W +L
Sbjct: 10 LQRLDS---QETQMETFAGLDDQIERIKGLLREIAEFF----GDRGSISHVAFSTWIDKL 62
Query: 174 IDIVYSMEDCIDNFVVHMENQSASDQTILIDQCKIELENLEKSLLHVRNQMLEDKKGKAV 353
IV M+D +D F++ ++ Q SD+ + +IEL+ E SL ++ E K
Sbjct: 63 KKIVSDMDDQVDEFIIQIDRQIGSDRLASTECFRIELQKSESSLFETIQRIRELKNSTVT 122
Query: 354 KPESLDVEE-----------------NTSKADAEPGE---------------ASSSPTSF 437
K E + E+ NT + E E ++SS F
Sbjct: 123 KEEKREKEDSNEQSHGREEGKMRLSSNTIEGPKEENEGNMEQFHEQEEGQTLSASSSVPF 182
Query: 438 KVYLSNLPYFLQSCLKYCCIFPENYAIPKGRLVRLLVAEGLIQEKPGQIAEDIAKENIKE 617
K+ +LPY+LQSCL YCCIFPENY I KG+L+RLLVAEGL QEK GQ+ ED+A+ NI E
Sbjct: 183 KLKYDSLPYYLQSCLMYCCIFPENYWINKGKLIRLLVAEGLTQEKTGQLLEDVAEGNINE 242
Query: 618 LANLGVLHVDGNKLKVPETIRAFMLCM----MGEEGFIVSCTSSDINVPPTTRRLSIHXX 785
L N G+L K + P + F + + E F +S+ +P R+ +
Sbjct: 243 LVNQGMLKF---KEEHPGSRPTFTVSSPYRKLLHENFKTGHANSNSTIPHIACRV-LASD 298
Query: 786 XXXXXXXXXXXXXRSLFLFGIRSLSDAALDSLRPVFHEKKFLRVLDLEDVHIKNIPDELG 965
RSLFLFG + D L KFLRVLDLED IK++PDE+G
Sbjct: 299 MTKIGHSLNNLRPRSLFLFGNQGHFDGHWLDLTWA----KFLRVLDLEDTKIKSLPDEVG 354
Query: 966 DMIHLRYLGLKNSDIDALPESVTNLRNLQTLDIRWNGKITDISSSILKLVQLRHLKMFKN 1145
D++HL YLGLK+ + LP + LR LQTLDIRW K++ +S IL L LRHLKM
Sbjct: 355 DLVHLTYLGLKHCQANELPARLGKLRALQTLDIRWCWKLSAVSPDILNLAGLRHLKMSC- 413
Query: 1146 WGINGVREILVPTDIRRSENLQTFTXXXXXXXXXXXXXXXTQLKRLGVMDVAADHVSDLS 1325
ING + +P I R NL T T TQL RLGV DVA ++ + +
Sbjct: 414 --ING-SGMKLPEGIGRLRNLLTLTGLCAGGGIAGELDRLTQLTRLGVADVAEENAAQVY 470
Query: 1326 VSIMKMKGLHSLSLEAKSSYXXXXXXXXXXXXXXXALQKLRLEGRLEKMPSWFSLMENLI 1505
+I+KM+GL LSLEA ++ L+KLRLEG LEK+PSW ME L
Sbjct: 471 AAIIKMQGLLCLSLEAHHAFNKQQLVLLELFSPPPLLRKLRLEGALEKIPSWLGSMEKLT 530
Query: 1506 KLRLGFSHLSEDPALVLQFLPNLKHLTLWRAYDGKQIGKEFCGVGRFPKLEVLVIASHVL 1685
KLRLGFSHLSE P LVLQ LPNLK LTLW AYD KQ+GKEFC G FPKLEVL IAS+VL
Sbjct: 531 KLRLGFSHLSEIPMLVLQILPNLKILTLWHAYDTKQLGKEFCKAGGFPKLEVLRIASYVL 590
Query: 1686 EEWTELEEGALPNLKYLHMHNCLRLRMLPEGLQHIATLQELKLIPLLDDHLERLKPDGGE 1865
EEWTELEEGALP+L+ LH HNC LR+LPEGLQ++ TL++LKL+PLLD+H ERLKPDGGE
Sbjct: 591 EEWTELEEGALPSLQCLHFHNCSSLRILPEGLQYVTTLKQLKLLPLLDEHAERLKPDGGE 650
Query: 1866 ENHKIRNIPLISFMPTSVVEQLMRGHPAGQ 1955
EN+KIR+I ISF+P SV+ + HP Q
Sbjct: 651 ENYKIRHIATISFIPMSVLS--LVSHPCHQ 678
>ref|XP_003633825.1| PREDICTED: disease resistance protein RPM1-like [Vitis vinifera]
Length = 690
Score = 416 bits (1069), Expect = e-113
Identities = 258/652 (39%), Positives = 361/652 (55%), Gaps = 22/652 (3%)
Frame = +3
Query: 9 RLKSVLLEENQKISSLEDQTQILISLLDEIGGFFESTDGTTGKNCELETRWFRELIDIVY 188
RL S+LL+EN+ + ++DQ + LIS LD +G F D G+ W EL ++
Sbjct: 12 RLNSMLLQENEILLGVKDQIESLISKLDAVGSFTGDADRILGRE-SCHQSWASELRGMLC 70
Query: 189 SMEDCIDNFVVHMENQSASDQTILIDQCKIELENLEKSLLHVRNQ--------MLEDKKG 344
+ED +D+F++ + Q+ D+ I + EL+N+ + + N+ MLE+ +
Sbjct: 71 EVEDFVDDFIIKVYGQTEKDREESIAEFGNELQNINSRVSEILNRRPRLEASLMLEEIQR 130
Query: 345 KAVKPESLDVEENTSKADAEPGEASSSPTSFKVYLSNLPYFLQSCLKYCCIFPENYAIPK 524
D ++ T+ A S + NLPY L+ L +C FP Y K
Sbjct: 131 FLRSNFVFDEDKETALA------------SCMLNYINLPYDLKLSLLFCRAFPGYYRKTK 178
Query: 525 GRLVRLLVAEGLIQEKPGQIAEDIAKENIKELANLGVLHVDG---NKLKVPETIRAFMLC 695
G LV +LVA GLIQEKPG++ ED A+ENI+ L +LG+L D + +VP L
Sbjct: 179 GALVPVLVAAGLIQEKPGELMEDKAQENIERLLDLGMLEQDDVGYDCFRVPSPYYELSLS 238
Query: 696 MMGEEGFIVSCTSSDINVPPTTRRLSIHXXXXXXXXXXXXXXXRSLFLFGIRSLSDAALD 875
+M E F +S +SD + PT +SIH SLF+ LS A+ D
Sbjct: 239 VMNAEDFFISTANSDSIILPTAHHVSIHDAENTITNMNSLL--HSLFVSAKDGLSKASSD 296
Query: 876 SLRPVFHEKKFLRVLDLEDVHIKNIPDELGDMIHLRYLGLKNSDIDALPESVTNLRNLQT 1055
L V + K LRVLDLE+ +K +PDE+G +++LRYLG+++S I+ LPES+ NLRNLQT
Sbjct: 297 CLETVLYNAKLLRVLDLENTKLKTLPDEVGKLVNLRYLGVRHSQINELPESIRNLRNLQT 356
Query: 1056 LDIRWNGKITDISSSILKLVQLRHLKMFKNWGINGVREILVPTDIRRSENLQTFTXXXXX 1235
LDI +G ++S+ +L L QLRHLKM + NG E+ VP I R NLQT
Sbjct: 357 LDISCSGNEFELSNGVLNLAQLRHLKMCRTVN-NG--EVRVPRGISRLRNLQTLEGIYAG 413
Query: 1236 XXXXXXXXXXTQLKRLGVMDVAADHVSDLSVSIMKMKGLHSLSLEAKSS----------- 1382
TQL+ L V V+ DH +L S+ K+ GL +LSL A+ +
Sbjct: 414 GGIAKELGNMTQLRSLDVRSVSDDHADELYASVTKLTGLRNLSLTAEWNGIAYDSSSPLP 473
Query: 1383 YXXXXXXXXXXXXXXXALQKLRLEGRLEKMPSWFSLMENLIKLRLGFSHLSEDPALVLQF 1562
Y L+ L L+G L +MP W MENL +L + SHLSE+P +LQF
Sbjct: 474 YENSLFPCLESFSPPPLLETLELKGCLTEMPLWLGSMENLTRLVMASSHLSENPTTILQF 533
Query: 1563 LPNLKHLTLWRAYDGKQIGKEFCGVGRFPKLEVLVIASHVLEEWTELEEGALPNLKYLHM 1742
LPNLK+L+++ AY GK++ +EF G FPKLE L I S L EWTE+EEGALP LK L+
Sbjct: 534 LPNLKYLSMFHAYKGKRMEREFFRAGGFPKLEYLKIVSRNLVEWTEMEEGALPCLKQLYF 593
Query: 1743 HNCLRLRMLPEGLQHIATLQELKLIPLLDDHLERLKPDGGEENHKIRNIPLI 1898
NC+RL LPEGLQH+ATLQ+L L + D RL P+GG +N+KI++IPL+
Sbjct: 594 WNCMRLMGLPEGLQHVATLQKLVLFNVHGDLTRRLNPNGGPQNYKIKHIPLV 645
>ref|XP_002318063.1| predicted protein [Populus trichocarpa] gi|222858736|gb|EEE96283.1|
predicted protein [Populus trichocarpa]
Length = 583
Score = 411 bits (1057), Expect = e-112
Identities = 221/396 (55%), Positives = 274/396 (69%)
Frame = +3
Query: 735 SDINVPPTTRRLSIHXXXXXXXXXXXXXXXRSLFLFGIRSLSDAALDSLRPVFHEKKFLR 914
SD ++P RR+ + RSLFLFG + LS + F KFLR
Sbjct: 177 SDFSIPRKARRI-LTPDMTKVAQSLNNIQPRSLFLFGKQELSGENWLN----FEGAKFLR 231
Query: 915 VLDLEDVHIKNIPDELGDMIHLRYLGLKNSDIDALPESVTNLRNLQTLDIRWNGKITDIS 1094
VLDLED I+ +PDE+ D+IHL YLGLKN+DI+ LP+ + NLR LQ+LDIRW G +T++S
Sbjct: 232 VLDLEDAKIRRLPDEVADLIHLAYLGLKNNDINELPDRLGNLRALQSLDIRWCGDLTELS 291
Query: 1095 SSILKLVQLRHLKMFKNWGINGVREILVPTDIRRSENLQTFTXXXXXXXXXXXXXXXTQL 1274
+ ILKLV+LRHLKMFK+ ++G++ +P + +L T T TQL
Sbjct: 292 TEILKLVRLRHLKMFKSRNVSGMK---LPGGVGTLGSLLTLTGVHAGGEIAGELRKLTQL 348
Query: 1275 KRLGVMDVAADHVSDLSVSIMKMKGLHSLSLEAKSSYXXXXXXXXXXXXXXXALQKLRLE 1454
+RLGVMDVA ++ ++L SIM M+GL SLSLEAK ++ L+KLRLE
Sbjct: 349 RRLGVMDVAEENANELYASIMVMQGLLSLSLEAKHTFDQGHLVLRDSFSPPSILRKLRLE 408
Query: 1455 GRLEKMPSWFSLMENLIKLRLGFSHLSEDPALVLQFLPNLKHLTLWRAYDGKQIGKEFCG 1634
G LEK P+W ME+L KLRLGFSHLSE+P LVLQ LPNL+ LTLW AYDG+Q+GK+FC
Sbjct: 409 GLLEKTPNWLGSMESLTKLRLGFSHLSENPTLVLQVLPNLEKLTLWHAYDGRQLGKDFCK 468
Query: 1635 VGRFPKLEVLVIASHVLEEWTELEEGALPNLKYLHMHNCLRLRMLPEGLQHIATLQELKL 1814
G FPKLEVL+IAS VLEEWTELEEGALP LKYLH HNCL LRMLPEGLQ + T++EL L
Sbjct: 469 PGGFPKLEVLIIASRVLEEWTELEEGALPRLKYLHFHNCLNLRMLPEGLQFVTTVEELVL 528
Query: 1815 IPLLDDHLERLKPDGGEENHKIRNIPLISFMPTSVV 1922
+PLLD+H ERLKPDGG EN+KIRNIP I+F+P SVV
Sbjct: 529 LPLLDEHEERLKPDGGLENYKIRNIPKITFIPASVV 564
Score = 73.2 bits (178), Expect = 3e-10
Identities = 47/148 (31%), Positives = 75/148 (50%), Gaps = 10/148 (6%)
Frame = +3
Query: 3 LQRLKSVLLEENQKISSLEDQTQILISLLDEIGGFFESTDGTTGKNCELETRWFRELIDI 182
LQRL+S+LL E + + LEDQ +++ + EIG F+ T G +RW EL ++
Sbjct: 10 LQRLESLLLRETETFTGLEDQMKMVKGTIKEIGCFYGDRGNTEGNAAF--SRWETELKNL 67
Query: 183 VYSMEDCIDNFVVHMENQSASDQTILIDQCKIELENLEKSLLHVRNQMLEDKKGKA---- 350
++D +D FV+ M+ Q SD+ LID + EL+ +E L ++M E K
Sbjct: 68 FDDLDDHVDEFVIQMDQQQGSDRLGLIDCFRAELQKIESRLAETTHKMKELKNSSTTAGG 127
Query: 351 ------VKPESLDVEENTSKADAEPGEA 416
+K +S EE ++ D+ EA
Sbjct: 128 MGEKSDIKEQSHGEEEVQTEVDSIASEA 155