BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00010304
         (1650 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 cataly...   524   e-163
ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 cataly...   518   e-162
ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [R...   503   e-157
ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit...   502   e-157
ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|2...   501   e-156

>ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit-like [Cucumis
            sativus] gi|449515377|ref|XP_004164726.1| PREDICTED:
            NEDD8-activating enzyme E1 catalytic subunit-like
            [Cucumis sativus]
          Length = 449

 Score =  524 bits (1350), Expect(2) = e-163
 Identities = 250/311 (80%), Positives = 276/311 (88%)
 Frame = +3

Query: 207  LDSIEARSYINSVACSFLEYGSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPCFECT 386
            LDSIEARSYINSVACSFLEY SDD P EETIKPMVDGGTEGFKGHARVILPGVTPCFECT
Sbjct: 139  LDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECT 198

Query: 387  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDVVHSGKAFDADDPEHMQWIYSEALK 566
            IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWD VHSGK+FD DD EHM+W+YSEALK
Sbjct: 199  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDDSEHMKWVYSEALK 258

Query: 567  RAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNG 746
            RAELFGIPGVTYSLTQGVVK                CALE LKIV+ CS+ +SNYLTYNG
Sbjct: 259  RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNG 318

Query: 747  LEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQGKT 926
             EGLHTK+TEFV+DKDCLVCGPG+L+EL++SITLQKF++L+ +HPKLLLSK SVT++GK 
Sbjct: 319  AEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVTHRGKN 378

Query: 927  LYMQAPPVLEEMTRSNLKLPLFDLMDKVSKDVIHVTGMTNKDDKKTSCLRKLRIVFKGVD 1106
            LYMQAPPVLEEMTRSNL +PLFDLM KV KDV+HVTG+ +K+DKKTSCLRKLR+ FKGVD
Sbjct: 379  LYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVD 438

Query: 1107 GITDLDMASGA 1139
            G+TD+D ASGA
Sbjct: 439  GVTDMDTASGA 449



 Score = 79.3 bits (194), Expect(2) = e-163
 Identities = 36/43 (83%), Positives = 42/43 (97%)
 Frame = +1

Query: 1   EVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDS 129
           EVAAKRVMERVSGVNI+PHFCRIEDK+++FY +F+IIALGLDS
Sbjct: 99  EVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLDS 141


>ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Vitis
            vinifera] gi|297736997|emb|CBI26198.3| unnamed protein
            product [Vitis vinifera]
          Length = 449

 Score =  518 bits (1335), Expect(2) = e-162
 Identities = 244/311 (78%), Positives = 272/311 (87%)
 Frame = +3

Query: 207  LDSIEARSYINSVACSFLEYGSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPCFECT 386
            LDSIEARSYIN+VACSFLEY SDDKP EETIKPMVDGGTEGFKGHARVI+PGVTPCFECT
Sbjct: 139  LDSIEARSYINAVACSFLEYDSDDKPLEETIKPMVDGGTEGFKGHARVIIPGVTPCFECT 198

Query: 387  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDVVHSGKAFDADDPEHMQWIYSEALK 566
            IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWD VHSGKAFD DDPEHM+W+YSEA+K
Sbjct: 199  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDPEHMKWVYSEAVK 258

Query: 567  RAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNG 746
            RAELFGIPGVTYSLTQGVVK                CALE LKIV+ CS+ +SNYLTYNG
Sbjct: 259  RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNG 318

Query: 747  LEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQGKT 926
             EGLHTK+TEFV+D DCL+CGPG+L+EL+TS+TLQKFM+ + EHPKLLLSK SV ++ K 
Sbjct: 319  AEGLHTKVTEFVKDMDCLICGPGVLIELDTSVTLQKFMDQLQEHPKLLLSKASVKHREKN 378

Query: 927  LYMQAPPVLEEMTRSNLKLPLFDLMDKVSKDVIHVTGMTNKDDKKTSCLRKLRIVFKGVD 1106
            LY+QAPPVLEE+TRSNL LPLFDLM K+ KD++HVTG T K+DKKTSCLRKLR+VFKG+D
Sbjct: 379  LYIQAPPVLEELTRSNLNLPLFDLMGKIRKDIVHVTGSTTKNDKKTSCLRKLRVVFKGID 438

Query: 1107 GITDLDMASGA 1139
            G TD+D A GA
Sbjct: 439  GATDMDTAGGA 449



 Score = 83.2 bits (204), Expect(2) = e-162
 Identities = 39/43 (90%), Positives = 41/43 (95%)
 Frame = +1

Query: 1   EVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDS 129
           EVAAKRVMERVSGVNIVPHFCRIEDKD++FY  FNIIALGLDS
Sbjct: 99  EVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNHFNIIALGLDS 141


>ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [Ricinus communis]
            gi|223543819|gb|EEF45347.1| ubiquitin-activating enzyme
            E1c, putative [Ricinus communis]
          Length = 449

 Score =  503 bits (1296), Expect(2) = e-157
 Identities = 233/311 (74%), Positives = 271/311 (87%)
 Frame = +3

Query: 207  LDSIEARSYINSVACSFLEYGSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPCFECT 386
            LDSIEARSYIN+VACSFLEY S+D P ++T+KPMVDGGTEGFKGHARVI+PG+TPCFECT
Sbjct: 139  LDSIEARSYINNVACSFLEYDSEDNPRDDTMKPMVDGGTEGFKGHARVIMPGITPCFECT 198

Query: 387  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDVVHSGKAFDADDPEHMQWIYSEALK 566
            IWLFPPQVKFPLCTLAETPRT AHCIEYAHLIKW+ VHSGKAFD DDPEHM+W+Y+EA+K
Sbjct: 199  IWLFPPQVKFPLCTLAETPRTPAHCIEYAHLIKWNEVHSGKAFDPDDPEHMKWVYTEAVK 258

Query: 567  RAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNG 746
            RAELFGI GVTYSLTQGVVK                CALE LKI + CS+ +SNYLTYNG
Sbjct: 259  RAELFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIASGCSKTLSNYLTYNG 318

Query: 747  LEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQGKT 926
            +EGLH K+TEFV+DKDCL CGPG+L+EL+TS+ LQKF++L+ EHPKL LS+ SVT++GK 
Sbjct: 319  VEGLHIKVTEFVKDKDCLACGPGVLIELDTSVALQKFIDLLEEHPKLFLSRASVTHRGKN 378

Query: 927  LYMQAPPVLEEMTRSNLKLPLFDLMDKVSKDVIHVTGMTNKDDKKTSCLRKLRIVFKGVD 1106
            LYMQAPPVLEEMTRSNL LPLF+LM KV KD++HVTGMT ++ KKTSC+RKLR+VFKG++
Sbjct: 379  LYMQAPPVLEEMTRSNLSLPLFELMGKVPKDIVHVTGMTGQNGKKTSCVRKLRVVFKGME 438

Query: 1107 GITDLDMASGA 1139
            GITD+DMA GA
Sbjct: 439  GITDMDMAGGA 449



 Score = 81.3 bits (199), Expect(2) = e-157
 Identities = 38/43 (88%), Positives = 41/43 (95%)
 Frame = +1

Query: 1   EVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDS 129
           EVAAKRVMERVSGVNIVPHFCRIEDK+LDFY +F+II LGLDS
Sbjct: 99  EVAAKRVMERVSGVNIVPHFCRIEDKELDFYNDFSIIVLGLDS 141


>ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit [Medicago truncatula]
            gi|355511444|gb|AES92586.1| NEDD8-activating enzyme E1
            catalytic subunit [Medicago truncatula]
          Length = 454

 Score =  502 bits (1292), Expect(2) = e-157
 Identities = 238/311 (76%), Positives = 266/311 (85%)
 Frame = +3

Query: 207  LDSIEARSYINSVACSFLEYGSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPCFECT 386
            LDSIEARSYIN+VACSFLEY SDD P EETIKPMVDGGTEGFKGHARVI+PG+TPCFECT
Sbjct: 144  LDSIEARSYINTVACSFLEYDSDDNPREETIKPMVDGGTEGFKGHARVIMPGITPCFECT 203

Query: 387  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDVVHSGKAFDADDPEHMQWIYSEALK 566
            IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWD VH G  FD D+PE+M+W+Y EA+K
Sbjct: 204  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHRGVPFDPDNPENMKWVYDEAVK 263

Query: 567  RAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNG 746
            RAELFGIPGVTYS TQGVVK                CALE LKI T CS+ +SNYLTYNG
Sbjct: 264  RAELFGIPGVTYSFTQGVVKNIIPAIASTNAIISAACALETLKIATECSKTLSNYLTYNG 323

Query: 747  LEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQGKT 926
             EGLHTK+TEF RDKDCLVCGPGI +EL+ SITLQKFM+L+ EHPKL LSK SVT++GK 
Sbjct: 324  SEGLHTKVTEFERDKDCLVCGPGIRIELDPSITLQKFMDLLEEHPKLRLSKASVTHRGKN 383

Query: 927  LYMQAPPVLEEMTRSNLKLPLFDLMDKVSKDVIHVTGMTNKDDKKTSCLRKLRIVFKGVD 1106
            LYMQAPPVLEEMTRSNL L LF+LM K+ KD++HV GMT+K+D+KTSCLRKLR+VFKG+D
Sbjct: 384  LYMQAPPVLEEMTRSNLTLSLFNLMGKLPKDIVHVNGMTSKNDQKTSCLRKLRVVFKGID 443

Query: 1107 GITDLDMASGA 1139
            G+TD+D A GA
Sbjct: 444  GVTDMDTAGGA 454



 Score = 80.1 bits (196), Expect(2) = e-157
 Identities = 36/43 (83%), Positives = 42/43 (97%)
 Frame = +1

Query: 1   EVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDS 129
           EVAAKRVMER+SGVNIVPHFCRIEDK+++FY +F+IIALGLDS
Sbjct: 104 EVAAKRVMERISGVNIVPHFCRIEDKEIEFYNDFSIIALGLDS 146


>ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|222851659|gb|EEE89206.1|
            predicted protein [Populus trichocarpa]
          Length = 449

 Score =  501 bits (1291), Expect(2) = e-156
 Identities = 235/311 (75%), Positives = 265/311 (85%)
 Frame = +3

Query: 207  LDSIEARSYINSVACSFLEYGSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPCFECT 386
            LDS+EARSYIN+VAC FLEY SDD P EET+KPMVDGGTEGFKGHARVI+PG TPCFECT
Sbjct: 139  LDSVEARSYINAVACGFLEYDSDDNPIEETVKPMVDGGTEGFKGHARVIIPGSTPCFECT 198

Query: 387  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDVVHSGKAFDADDPEHMQWIYSEALK 566
            IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWD VHSGK FD DDPEHM+W+Y+EA+K
Sbjct: 199  IWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKTFDPDDPEHMKWVYTEAVK 258

Query: 567  RAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNG 746
            RAELFGI GVTYSLTQGVVK                C LE LK+ + CS+ +SNYLTYNG
Sbjct: 259  RAELFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKLASGCSKTLSNYLTYNG 318

Query: 747  LEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQGKT 926
            +EGLH K+TEFV+DKDCLVCGPG+LLEL+TS+TLQKF++++ EHPKLLLSK SV ++   
Sbjct: 319  VEGLHIKVTEFVKDKDCLVCGPGVLLELDTSVTLQKFIDMLEEHPKLLLSKASVRHRATN 378

Query: 927  LYMQAPPVLEEMTRSNLKLPLFDLMDKVSKDVIHVTGMTNKDDKKTSCLRKLRIVFKGVD 1106
            LYMQAPPVLEEMTRSNL LPLF+LM K  KD++HVTG T+KDDKKTSCLRKL +VFKG D
Sbjct: 379  LYMQAPPVLEEMTRSNLNLPLFELMGKFPKDIVHVTGTTSKDDKKTSCLRKLCLVFKGAD 438

Query: 1107 GITDLDMASGA 1139
             +TDLDMA GA
Sbjct: 439  AVTDLDMAVGA 449



 Score = 79.7 bits (195), Expect(2) = e-156
 Identities = 37/43 (86%), Positives = 41/43 (95%)
 Frame = +1

Query: 1   EVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDS 129
           EVAAKRVMERVSGVNIVPHFCRIEDK++DFY++F II LGLDS
Sbjct: 99  EVAAKRVMERVSGVNIVPHFCRIEDKEIDFYKDFIIIVLGLDS 141