BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00009964
         (857 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      425   e-117
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   410   e-112
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       397   e-108
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   362   5e-98
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   345   1e-92

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  425 bits (1093), Expect = e-117
 Identities = 203/284 (71%), Positives = 236/284 (83%)
 Frame = +1

Query: 1   KQPKKAATRXXXXXXXXXXXPYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQ 180
           KQ KKAA             PYDDIKQLIR ELA+RLQWGYK T EEQ+AE+  L HSL+
Sbjct: 6   KQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLR 65

Query: 181 HMKIANEVETLDSQLYEAPMSFLKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERA 360
            MKIA EVETLDSQLYE P+ FLKI+ GS +K SCCYFK+DS TLD+AEIAMLDLYCERA
Sbjct: 66  QMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERA 125

Query: 361 QIKDGNNVLDLGCGQGAITLFVAQKYKQCRVKAITNSISQKEYIDEQCRARNLVNVEVVL 540
           QI+DG +VLDLGCGQGA+TL VAQKYK CRV A+TNS+SQKEYI+E+ R RNL+NVEV L
Sbjct: 126 QIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKL 185

Query: 541 ADITTYETDDTFDRILAIGLFEHMQNYKLLLEKISKWMAKDGLLFVDHVCHKTLAYQYKA 720
           ADITT+E  +T+DRIL I LFEHM+NY+LLL KIS+W++KDGLLF++H+CHKT AY Y+ 
Sbjct: 186 ADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEP 245

Query: 721 LDEDDWFTEYIFPAGTIFFPSASFLLYFQDDVSVVNHWILNGNH 852
           LD+DDWFTEY+FPAGT+  PSASF LYFQDDVSVVNHW L+G H
Sbjct: 246 LDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKH 289


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  410 bits (1054), Expect = e-112
 Identities = 193/282 (68%), Positives = 233/282 (82%)
 Frame = +1

Query: 7   PKKAATRXXXXXXXXXXXPYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHM 186
           PKKA              P D+IK+LIRI+L +RLQWG KST EEQ+A+++ L HSL+ M
Sbjct: 10  PKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQM 69

Query: 187 KIANEVETLDSQLYEAPMSFLKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQI 366
           KIA EVETLD Q+YE P+ FLKI+ GS +K SCCYFK+DS TLD+AEIAML+LYCERAQI
Sbjct: 70  KIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQI 129

Query: 367 KDGNNVLDLGCGQGAITLFVAQKYKQCRVKAITNSISQKEYIDEQCRARNLVNVEVVLAD 546
           KDG++VLDLGCGQGA+TL+VAQKYK  RV A+TNS+SQKE+I+E+ R RNL NVEV+LAD
Sbjct: 130 KDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLAD 189

Query: 547 ITTYETDDTFDRILAIGLFEHMQNYKLLLEKISKWMAKDGLLFVDHVCHKTLAYQYKALD 726
           ITT++  DT+DRIL + LFEHM+NY+LLL KI +WMAKDGLLFV+H+CHKT AY Y+ +D
Sbjct: 190 ITTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPID 249

Query: 727 EDDWFTEYIFPAGTIFFPSASFLLYFQDDVSVVNHWILNGNH 852
           EDDWFTEY+FPAGT+  PSASF LYFQDDVSVVNHW L+G H
Sbjct: 250 EDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKH 291


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  397 bits (1019), Expect = e-108
 Identities = 184/264 (69%), Positives = 224/264 (84%)
 Frame = +1

Query: 61  PYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHMKIANEVETLDSQLYEAPM 240
           P D++K+L+RI+LA+RLQWGYKST EEQ+A+VL L HSL+HM IA EV+TLDS +YE P+
Sbjct: 19  PNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVDTLDSNMYEVPL 78

Query: 241 SFLKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQIKDGNNVLDLGCGQGAITL 420
            FLKI+ GS +K S CYFKDDS TLD+AEIAM+DL+CERAQIKDG++VLDLGCG G++T 
Sbjct: 79  DFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVLDLGCGHGSLTF 138

Query: 421 FVAQKYKQCRVKAITNSISQKEYIDEQCRARNLVNVEVVLADITTYETDDTFDRILAIGL 600
           +VAQKYK+  V A+TNS+SQKEYI+E+ R RNL NVEV+LADI T+E  DT+DRIL +GL
Sbjct: 139 YVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMADTYDRILVVGL 198

Query: 601 FEHMQNYKLLLEKISKWMAKDGLLFVDHVCHKTLAYQYKALDEDDWFTEYIFPAGTIFFP 780
           FEHM+NY LLL+KIS+WMAKDGLLFV HVCHKT A+  + +DEDDW +EY FPAGT+  P
Sbjct: 199 FEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSEYAFPAGTMIIP 258

Query: 781 SASFLLYFQDDVSVVNHWILNGNH 852
           SASF LYFQDD SVVNHW L+G H
Sbjct: 259 SASFFLYFQDDASVVNHWTLSGKH 282


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
           somniferum]
          Length = 351

 Score =  362 bits (930), Expect = 5e-98
 Identities = 171/264 (64%), Positives = 214/264 (81%)
 Frame = +1

Query: 61  PYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHMKIANEVETLDSQLYEAPM 240
           P  +I+QLIR+EL KRLQWGYK T EEQ++++L LVHSL+ MK+A E+E LD +LYEAPM
Sbjct: 19  PDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENLDLKLYEAPM 78

Query: 241 SFLKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQIKDGNNVLDLGCGQGAITL 420
            FLKI  GS MK+S  Y+ D+S TLD+AEIAMLDLY ERAQIKDG +VLDLGCG GA+ L
Sbjct: 79  EFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDLGCGLGAVAL 138

Query: 421 FVAQKYKQCRVKAITNSISQKEYIDEQCRARNLVNVEVVLADITTYETDDTFDRILAIGL 600
           F A K+K+C+   +T+S+ QK+YI+ +C+   L NV+V+LADITTYET++ FDRI A+ L
Sbjct: 139 FGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETEERFDRIFAVEL 198

Query: 601 FEHMQNYKLLLEKISKWMAKDGLLFVDHVCHKTLAYQYKALDEDDWFTEYIFPAGTIFFP 780
            EHM+NY+LLL+KIS+WM  DGLLFV+HVCHKTLAY Y+ +D +DW+T YIFPAGT+   
Sbjct: 199 IEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYIFPAGTLTLS 258

Query: 781 SASFLLYFQDDVSVVNHWILNGNH 852
           SAS LLYFQDDVSVVN W L+G H
Sbjct: 259 SASMLLYFQDDVSVVNQWTLSGKH 282


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
           gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
           [Thalictrum flavum]
          Length = 356

 Score =  345 bits (884), Expect = 1e-92
 Identities = 158/284 (55%), Positives = 218/284 (76%)
 Frame = +1

Query: 1   KQPKKAATRXXXXXXXXXXXPYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQ 180
           KQ KK A               ++I+ +++I++ KRL+WGYK T E+Q+A+++    SL+
Sbjct: 4   KQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLK 63

Query: 181 HMKIANEVETLDSQLYEAPMSFLKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERA 360
            M++A EV+TLD++LYE P+ FL I+ G T+K S  YFKD+S TLD++E+ M+DLYCERA
Sbjct: 64  GMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERA 123

Query: 361 QIKDGNNVLDLGCGQGAITLFVAQKYKQCRVKAITNSISQKEYIDEQCRARNLVNVEVVL 540
           QIKDG ++LDLGCG G++TL VAQKY+ C+V  ITNS+SQKE+I +QC+  +L NVE++L
Sbjct: 124 QIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIIL 183

Query: 541 ADITTYETDDTFDRILAIGLFEHMQNYKLLLEKISKWMAKDGLLFVDHVCHKTLAYQYKA 720
            D+T +ET+ T+DRI A+ L EHM+NY+L L+K+S W+A+ GLLFV+H CHK  AYQY+ 
Sbjct: 184 EDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEP 243

Query: 721 LDEDDWFTEYIFPAGTIFFPSASFLLYFQDDVSVVNHWILNGNH 852
           LDEDDW+TEYIFP+GT+   S+S LLYFQ+DVSVVNHW L+G H
Sbjct: 244 LDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKH 287