BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00009663
         (1942 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003632058.1| PREDICTED: probable pectinesterase/pectinest...   520   e-145
ref|XP_002527752.1| Pectinesterase-2 precursor, putative [Ricinu...   466   e-128
ref|NP_191460.1| pectinesterase 35 [Arabidopsis thaliana] gi|753...   466   e-128
ref|XP_002878254.1| pectinesterase family protein [Arabidopsis l...   454   e-125
emb|CBI39447.3| unnamed protein product [Vitis vinifera]              424   e-116

>ref|XP_003632058.1| PREDICTED: probable pectinesterase/pectinesterase inhibitor 35-like
            [Vitis vinifera]
          Length = 553

 Score =  520 bits (1340), Expect = e-145
 Identities = 279/519 (53%), Positives = 352/519 (67%), Gaps = 8/519 (1%)
 Frame = -2

Query: 1752 SSVHISTVSKXXXXXXAPFPCKDTLYPKACESQILSAKDASSTPITLDDVLNLSIKSVID 1573
            SS H STV K          CK T Y  AC+S +LS+ + +  P T  D+ +LS++  ++
Sbjct: 39   SSSHTSTVPKASMEAIVSAACKATFYQTACQSALLSSTNGA-VPQTQADLFDLSVQFSLN 97

Query: 1572 RATLAKSLAQNVSLMNQRENPNSFTALDDCVELLDHSIDQLNSILDS-KTTAPSPDDTQT 1396
            +A  A++   ++ L++ +      T  DDC+ELLD ++DQL ++ +  KT    PDD QT
Sbjct: 98   QARSARAHVHDLRLLDHKTQIVRGT--DDCMELLDDTLDQLTNVANRRKTLIEDPDDVQT 155

Query: 1395 WLSTAITHQTTCLDGLQTHKFFKEKELIHATLQSNIQHVSNSLVIHKSIVSAKTL-KVST 1219
            WLS A+T+Q TCL+ +QT++   +  L+    Q+    +SNSL +H S   +K   + +T
Sbjct: 156  WLSAALTNQATCLESIQTYQTGGQNGLMRPMAQNLTYSISNSLALHMSTRPSKEAQRTNT 215

Query: 1218 GG--RRLLSDDFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEALAFASLVGG 1045
             G  RRLLSD FP WV+  ERKLLE+S+++I   AVVA DG+GTH+TI EALA    + G
Sbjct: 216  AGHHRRLLSDRFPGWVTAAERKLLEASVEEIGATAVVAKDGSGTHKTIGEALAMVVTLEG 275

Query: 1044 GGRTVIHVTAGTYNENNIMVSAKQSNVMLVGDGKXXXXXXXXXXXXXXXXXXXXXSFGAG 865
             GRTVIHV AGTY+E  + + + Q NVMLVGDGK                     + G  
Sbjct: 276  EGRTVIHVKAGTYDEG-LKIPSSQKNVMLVGDGKGKTVIVGHKSYAGGSSTYDSATVGVM 334

Query: 864  GDGFIARDISFVNTAGPSKGQAVALVVTADKSVFYRCSIIGYQDTLYTLSNRQFYRETDI 685
            GDGFIARDI+  N AGP KGQAVAL V +D+SV +RCSIIGYQDTLYTLS RQFYRETDI
Sbjct: 335  GDGFIARDITIENDAGPGKGQAVALRVGSDRSVVFRCSIIGYQDTLYTLSKRQFYRETDI 394

Query: 684  YGTVDFIFGNSAVVFQSCNILPRSRGSDGKVFITAQGRTDPNQNTGISIHKCRIAGTGGN 505
            YGTVDFIFGNSAVVFQSCN+   +R S    F+TAQGR DPNQNTGISIH C+I   G  
Sbjct: 395  YGTVDFIFGNSAVVFQSCNL--NARKSSNNNFVTAQGREDPNQNTGISIHNCKITTEGST 452

Query: 504  TYLGRPWKKYSRTVYMQSFLDG-INPAGWFPWNG---MSTIYYGEYMNSGPGASTSGRVK 337
            TYLGRPWKKYSRTV MQS+LDG I P+GW+PW+G   +ST++YGEYMN+GPGASTSGRVK
Sbjct: 453  TYLGRPWKKYSRTVIMQSYLDGSIPPSGWYPWSGSFALSTLFYGEYMNAGPGASTSGRVK 512

Query: 336  WSGNHPSMTPAEASEFTVGSLISGNSWLPSTGIIFESGL 220
            W G    +T + A EFTVG  ISGN+WLPSTG+ F+SGL
Sbjct: 513  WGGYQGELTASVAQEFTVGEFISGNAWLPSTGVSFDSGL 551


>ref|XP_002527752.1| Pectinesterase-2 precursor, putative [Ricinus communis]
            gi|223532839|gb|EEF34613.1| Pectinesterase-2 precursor,
            putative [Ricinus communis]
          Length = 583

 Score =  466 bits (1198), Expect = e-128
 Identities = 267/517 (51%), Positives = 339/517 (65%), Gaps = 26/517 (5%)
 Frame = -2

Query: 1692 CKDTLYPKACESQILSAKDASSTPITLDDVLNLSIKSVIDRATLAKSLAQNVSLMNQ-RE 1516
            C D  Y  ACES + S K  ++ P T  ++ + S+   + +A  A  LA  VS  N  + 
Sbjct: 71   CNDIPYKSACESVLSSLK--TTIPTTPKELFDHSVHYTLKQARSAFELASTVSPSNNVKA 128

Query: 1515 NPNSFTA--------LDDCVELLDHSIDQLNSILDSKTTAP-SPDDTQTWLSTAITHQTT 1363
              N+  A        + DCVELL+ S+D L++I + K     + +D QTWLS A+T+Q T
Sbjct: 129  AANTHLAAVAGGGGGMIDCVELLEDSLDLLSTITNRKNNPKHTHNDVQTWLSAALTNQET 188

Query: 1362 CLDGLQTHKFF----KEKELIHATLQSNIQHVSNSLVIHKSIVSAKTLKVSTGGRRLLS- 1198
            C++ LQ  K      + +E+I  T ++  QH+SNSL ++ S     T + + GGR+LLS 
Sbjct: 189  CIESLQNDKLLFKLDRGQEMIR-TARNLSQHISNSLALYMSHYY-NTKESNKGGRKLLSG 246

Query: 1197 DDFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEALAFA---SLVG--GGGRT 1033
            DDFP WVS  ERKLLE+ + +I+ +AVVA DG+GTH TI EA+A     SL G  GGGR 
Sbjct: 247  DDFPAWVSESERKLLETPVAEIKAHAVVAKDGSGTHTTIGEAIAEVMSYSLAGEGGGGRN 306

Query: 1032 VIHVTAGTYNENNIMVSAKQSNVMLVGDGKXXXXXXXXXXXXXXXXXXXXXSFGAGGDGF 853
            VI+V AGTY+EN + +  KQ NVMLVGDGK                     +    GDGF
Sbjct: 307  VIYVKAGTYHEN-LKLPTKQKNVMLVGDGKGKSVITGSRSADDGYSTFQTATVSVMGDGF 365

Query: 852  IARDISFVNTAGPSKGQAVALVVTADKSVFYRCSIIGYQDTLYTLSNRQFYRETDIYGTV 673
            +A+ I+FVN+AGP+K QAVAL V +DKSV Y+CS+   QDTLYT S RQFYR+TDIYGT+
Sbjct: 366  MAKGITFVNSAGPNKHQAVALRVASDKSVIYQCSLEANQDTLYTHSKRQFYRDTDIYGTI 425

Query: 672  DFIFGNSAVVFQSCNILPRSRGSDGKVFITAQGRTDPNQNTGISIHKCRIAGTGGN--TY 499
            DFIFGNSAVV Q+CNI  R    D K ++TAQGRTDPNQNTGISIH C+I    G+  TY
Sbjct: 426  DFIFGNSAVVIQNCNIFARKPSGD-KNYVTAQGRTDPNQNTGISIHNCKITSESGSKVTY 484

Query: 498  LGRPWKKYSRTVYMQSFLDG-INPAGWFPWNG---MSTIYYGEYMNSGPGASTSGRVKWS 331
            LGRPW+KY+R V MQSFLDG INPAGWFPW+G   ++T+YY EYMNSGPGASTSGRVKW 
Sbjct: 485  LGRPWQKYARVVIMQSFLDGSINPAGWFPWSGSFALNTLYYAEYMNSGPGASTSGRVKWP 544

Query: 330  GNHPSMTPAEASEFTVGSLISGNSWLPSTGIIFESGL 220
            G    ++ A A  FTVG+ I GN WLPSTG+ F+SGL
Sbjct: 545  GYKGILSSAAAQTFTVGNFIEGNMWLPSTGVSFDSGL 581


>ref|NP_191460.1| pectinesterase 35 [Arabidopsis thaliana]
            gi|75311708|sp|Q9LYT5.1|PME35_ARATH RecName:
            Full=Probable pectinesterase/pectinesterase inhibitor 35;
            Includes: RecName: Full=Pectinesterase inhibitor 35;
            AltName: Full=Pectin methylesterase inhibitor 35;
            Includes: RecName: Full=Pectinesterase 35; Short=PE 35;
            AltName: Full=Pectin methylesterase 35; Short=AtPME35;
            Flags: Precursor gi|7529744|emb|CAB86929.1|
            pectinesterase precursor-like protein [Arabidopsis
            thaliana] gi|16974625|gb|AAL31215.1| AT3g59010/F17J16_60
            [Arabidopsis thaliana] gi|24111417|gb|AAN46858.1|
            At3g59010/F17J16_60 [Arabidopsis thaliana]
            gi|332646339|gb|AEE79860.1| pectinesterase 35
            [Arabidopsis thaliana]
          Length = 529

 Score =  466 bits (1198), Expect = e-128
 Identities = 250/465 (53%), Positives = 324/465 (69%), Gaps = 7/465 (1%)
 Frame = -2

Query: 1593 SIKSVIDRATLAKSLAQNVSLMNQRENPNSFTALDDCVELLDHSIDQLNSILDSKTTAPS 1414
            S++  +D A  A+SLA N++L ++         ++DC+ELLD ++D L  I+  K     
Sbjct: 69   SVQLSLDHALFARSLAFNLTLSHRTSQTLMLDPVNDCLELLDDTLDMLYRIVVIKRKDHV 128

Query: 1413 PDDTQTWLSTAITHQTTCLDGLQTHKFFKEKELIHATLQSNIQHV-SNSLVIHKSIVSAK 1237
             DD  TWLS A+T+Q TC   L     F ++ +   +   N+  + +NSL +  S     
Sbjct: 129  NDDVHTWLSAALTNQETCKQSLSEKSSFNKEGIAIDSFARNLTGLLTNSLDMFVSDKQKS 188

Query: 1236 TLKVS-TGGRRLLSD-DFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEALAF 1063
            +   + TGGR+LLSD DFPTWVS+ +RKLLE+S++++  +AVVA DG+GTH +++EALA 
Sbjct: 189  SSSSNLTGGRKLLSDHDFPTWVSSSDRKLLEASVEELRPHAVVAADGSGTHMSVAEALA- 247

Query: 1062 ASLVGGGGRTVIHVTAGTYNENNIMVSAKQSNVMLVGDGKXXXXXXXXXXXXXXXXXXXX 883
             SL  G GR+VIH+TAGTY EN + + +KQ NVMLVGDGK                    
Sbjct: 248  -SLEKGSGRSVIHLTAGTYKEN-LNIPSKQKNVMLVGDGKGKTVIVGSRSNRGGWNTYQS 305

Query: 882  XSFGAGGDGFIARDISFVNTAGPSKGQAVALVVTADKSVFYRCSIIGYQDTLYTLSNRQF 703
             +  A GDGFIARDI+FVN+AGP+  QAVAL V +D+SV YRCSI GYQD+LYTLS RQF
Sbjct: 306  ATVAAMGDGFIARDITFVNSAGPNSEQAVALRVGSDRSVVYRCSIDGYQDSLYTLSKRQF 365

Query: 702  YRETDIYGTVDFIFGNSAVVFQSCNILPRSRGSDGKVFITAQGRTDPNQNTGISIHKCRI 523
            YRETDI GTVDFIFGNSAVVFQSCN++ R +GS  + ++TAQGR+DPNQNTGISIH CRI
Sbjct: 366  YRETDITGTVDFIFGNSAVVFQSCNLVSR-KGSSDQNYVTAQGRSDPNQNTGISIHNCRI 424

Query: 522  AGTGGNTYLGRPWKKYSRTVYMQSFLDG-INPAGWFPWN---GMSTIYYGEYMNSGPGAS 355
             G+   TYLGRPWK+YSRTV MQSF+DG I+P+GW PW+    + T+YYGE+ NSGPG+S
Sbjct: 425  TGS-TKTYLGRPWKQYSRTVVMQSFIDGSIHPSGWSPWSSNFALKTLYYGEFGNSGPGSS 483

Query: 354  TSGRVKWSGNHPSMTPAEASEFTVGSLISGNSWLPSTGIIFESGL 220
             SGRV W+G HP++T  EA  FTV   I GNSWLPSTG++F+SGL
Sbjct: 484  VSGRVSWAGYHPALTLTEAQGFTVSGFIDGNSWLPSTGVVFDSGL 528


>ref|XP_002878254.1| pectinesterase family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297324092|gb|EFH54513.1| pectinesterase family protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 525

 Score =  454 bits (1168), Expect = e-125
 Identities = 248/468 (52%), Positives = 321/468 (68%), Gaps = 10/468 (2%)
 Frame = -2

Query: 1593 SIKSVIDRATLAKSLAQNVSLMNQRENPNSFTALDDCVELLDHSIDQLNSILDSKTTAPS 1414
            S++  ++ A  A+S A N++L ++         ++DC+ELLD ++D L+ I+  K     
Sbjct: 65   SVQLSLEDALFARSFAFNLTLSHRTSQSLMLDPVNDCLELLDDTLDMLSRIVVIKRKDHV 124

Query: 1413 PDDTQTWLSTAITHQTTCLDGLQTHKFFKEKELIHATLQSNIQHV-SNSLVIHKSIVSAK 1237
             DD  TWLS A+T+Q TC   L     F +  +   +   N+  + +NSL +    VS K
Sbjct: 125  NDDVHTWLSAALTNQETCKQSLSEKSSFNKDGIAIDSFARNLTGLLTNSLDM---FVSDK 181

Query: 1236 TLKVS----TGGRRLLSD-DFPTWVSTGERKLLESSIKDIEVNAVVAMDGTGTHRTISEA 1072
                S    TGGR+LLS+ DFP+W S  +RKLLE+S++++  +AVVA DG+GTH +I+EA
Sbjct: 182  RKSSSSSRLTGGRKLLSEHDFPSWFSMSDRKLLEASVEELRPHAVVAADGSGTHMSIAEA 241

Query: 1071 LAFASLVGGGGRTVIHVTAGTYNENNIMVSAKQSNVMLVGDGKXXXXXXXXXXXXXXXXX 892
            LA  SL  G GR+VIH+ AGTY EN + + +KQ NVMLVGDGK                 
Sbjct: 242  LA--SLEKGSGRSVIHLAAGTYKEN-LNIPSKQKNVMLVGDGKGKTVIIGSRSNRGGWNT 298

Query: 891  XXXXSFGAGGDGFIARDISFVNTAGPSKGQAVALVVTADKSVFYRCSIIGYQDTLYTLSN 712
                +  A GDGFIARDI+FVN+AGP+  QAVAL V +D+SV YRCSI GYQD+LYTLS 
Sbjct: 299  YQSATVAAMGDGFIARDITFVNSAGPNSEQAVALRVGSDRSVVYRCSIDGYQDSLYTLSK 358

Query: 711  RQFYRETDIYGTVDFIFGNSAVVFQSCNILPRSRGSDGKVFITAQGRTDPNQNTGISIHK 532
            RQFYRETDI GTVDFIFGNSAVVFQSCN++ R +GS  + ++TAQGR+DPNQNTGISIH 
Sbjct: 359  RQFYRETDITGTVDFIFGNSAVVFQSCNLVSR-KGSSDENYVTAQGRSDPNQNTGISIHN 417

Query: 531  CRIAGTGGNTYLGRPWKKYSRTVYMQSFLDG-INPAGWFPWN---GMSTIYYGEYMNSGP 364
            CRI G+   TYLGRPWK+YSRTV MQSF+DG I+P+GW PW+    + T+YYGE+ NSGP
Sbjct: 418  CRITGS-TKTYLGRPWKQYSRTVVMQSFIDGSIHPSGWSPWSSSFALKTLYYGEFGNSGP 476

Query: 363  GASTSGRVKWSGNHPSMTPAEASEFTVGSLISGNSWLPSTGIIFESGL 220
            G+S SGRV W+G HP++T  EA  FTV   I GNSWLPSTG++F+SGL
Sbjct: 477  GSSVSGRVGWAGYHPALTLTEAQGFTVSGFIDGNSWLPSTGVVFDSGL 524


>emb|CBI39447.3| unnamed protein product [Vitis vinifera]
          Length = 432

 Score =  424 bits (1089), Expect = e-116
 Identities = 219/363 (60%), Positives = 259/363 (71%), Gaps = 4/363 (1%)
 Frame = -2

Query: 1296 SNIQHVSNSLVIHKSIVSAKTLKVSTGGRRLLSDDFPTWVSTGERKLLESSIKDIEVNAV 1117
            S+I   S++        ++ T   +   RRLLSD FP WV+  ERKLLE+S+++I   AV
Sbjct: 71   SSIFQCSSASTKRDQPTNSPTTNTAGHHRRLLSDRFPGWVTAAERKLLEASVEEIGATAV 130

Query: 1116 VAMDGTGTHRTISEALAFASLVGGGGRTVIHVTAGTYNENNIMVSAKQSNVMLVGDGKXX 937
            VA DG+GTH+TI EALA    + G GRTVIHV AGTY+E  + + + Q NVMLVGDGK  
Sbjct: 131  VAKDGSGTHKTIGEALAMVVTLEGEGRTVIHVKAGTYDEG-LKIPSSQKNVMLVGDGKGK 189

Query: 936  XXXXXXXXXXXXXXXXXXXSFGAGGDGFIARDISFVNTAGPSKGQAVALVVTADKSVFYR 757
                               + G  GDGFIARDI+  N AGP KGQAVAL V +D+SV +R
Sbjct: 190  TVIVGHKSYAGGSSTYDSATVGVMGDGFIARDITIENDAGPGKGQAVALRVGSDRSVVFR 249

Query: 756  CSIIGYQDTLYTLSNRQFYRETDIYGTVDFIFGNSAVVFQSCNILPRSRGSDGKVFITAQ 577
            CSIIGYQDTLYTLS RQFYRETDIYGTVDFIFGNSAVVFQSCN+   +R S    F+TAQ
Sbjct: 250  CSIIGYQDTLYTLSKRQFYRETDIYGTVDFIFGNSAVVFQSCNL--NARKSSNNNFVTAQ 307

Query: 576  GRTDPNQNTGISIHKCRIAGTGGNTYLGRPWKKYSRTVYMQSFLDG-INPAGWFPWNG-- 406
            GR DPNQNTGISIH C+I   G  TYLGRPWKKYSRTV MQS+LDG I P+GW+PW+G  
Sbjct: 308  GREDPNQNTGISIHNCKITTEGSTTYLGRPWKKYSRTVIMQSYLDGSIPPSGWYPWSGSF 367

Query: 405  -MSTIYYGEYMNSGPGASTSGRVKWSGNHPSMTPAEASEFTVGSLISGNSWLPSTGIIFE 229
             +ST++YGEYMN+GPGASTSGRVKW G    +T + A EFTVG  ISGN+WLPSTG+ F+
Sbjct: 368  ALSTLFYGEYMNAGPGASTSGRVKWGGYQGELTASVAQEFTVGEFISGNAWLPSTGVSFD 427

Query: 228  SGL 220
            SGL
Sbjct: 428  SGL 430