BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00009491
         (1656 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hy...   596   e-168
sp|Q42965.1|NRL4A_TOBAC RecName: Full=Bifunctional nitrilase/nit...   595   e-167
sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nit...   593   e-167
ref|XP_002328237.1| nitrilase 1 [Populus trichocarpa] gi|2228377...   593   e-167
ref|XP_004139500.1| PREDICTED: bifunctional nitrilase/nitrile hy...   592   e-166

>ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4B-like [Vitis
            vinifera]
          Length = 347

 Score =  596 bits (1537), Expect = e-168
 Identities = 286/353 (81%), Positives = 321/353 (90%)
 Frame = -2

Query: 1565 MALVPIPLIDNNSAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1386
            MAL+P  + D      P+ AEVDMG+DS+A TVRATVVQAST+FYDTPATL KAERL+++
Sbjct: 1    MALIPATVNDR-----PLFAEVDMGADSSAPTVRATVVQASTVFYDTPATLDKAERLLAE 55

Query: 1385 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1206
            A+ YGSQLVVFPEAFIGGYPRGSNFG+T+G+RT KG+E+FRKYHA+AIDVPGPEV+ L A
Sbjct: 56   ASSYGSQLVVFPEAFIGGYPRGSNFGVTIGNRTAKGREDFRKYHAAAIDVPGPEVDRLAA 115

Query: 1205 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGGGSTIP 1026
            MAGKYKVYLVMGVIER+GYTLYCT+LFFDSQGHYLGKHRK+MPT+LER+IWGFG GSTIP
Sbjct: 116  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKVMPTALERIIWGFGDGSTIP 175

Query: 1025 VFETAIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 846
            V+ET IGKIG  ICWEN+MPLLRTAMY KGIEIYCAPTADARD+WQ+SMTHIALEGGCFV
Sbjct: 176  VYETPIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADARDIWQSSMTHIALEGGCFV 235

Query: 845  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTVLAGPNYEGEALIS 666
            LSANQFCRRKDYPPPPEY FSG D DL+P+SVVCAGGSVIISPSGTVLAGPNY+GEALIS
Sbjct: 236  LSANQFCRRKDYPPPPEYEFSGAD-DLTPDSVVCAGGSVIISPSGTVLAGPNYDGEALIS 294

Query: 665  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 507
            ADLD GEI RAKFDFDVVGHYSRP+VL+L+V+D+PT PV FTSA+ KTE   K
Sbjct: 295  ADLDLGEIARAKFDFDVVGHYSRPEVLSLVVKDNPTKPVTFTSASVKTEDFQK 347


>sp|Q42965.1|NRL4A_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4A;
            Short=TNIT4A; AltName: Full=Cyanoalanine nitrilase A;
            AltName: Full=Nitrilase 4A gi|1171482|dbj|BAA09645.1|
            nitrilase [Nicotiana tabacum]
          Length = 349

 Score =  595 bits (1533), Expect = e-167
 Identities = 282/353 (79%), Positives = 319/353 (90%)
 Frame = -2

Query: 1565 MALVPIPLIDNNSAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1386
            MALVP P ++      P+ AEVDMG +S+  TVRATVVQASTIFYDTPATL KAERL+++
Sbjct: 1    MALVPTPAVNEG----PLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAE 56

Query: 1385 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1206
            AA YG+QLVVFPEAFIGGYPRGS FG+++G+RT KGKEEFRKYHASAIDVPGPEV+ L A
Sbjct: 57   AASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAA 116

Query: 1205 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGGGSTIP 1026
            MAGKYKVYLVMGVIER+GYTLYCT+LFFDSQGH+LGKHRK+MPT+LER+IWGFG GSTIP
Sbjct: 117  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIP 176

Query: 1025 VFETAIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 846
            V++T +GKIG  ICWEN+MPLLRTAMY KGIEIYCAPTAD+RD+WQASMTHIALEGGCFV
Sbjct: 177  VYDTPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFV 236

Query: 845  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTVLAGPNYEGEALIS 666
            LSANQFCRRKDYPPPPEY FSGT+EDL+P+S+VCAGGSVIISPSG VLAGPNY GEALIS
Sbjct: 237  LSANQFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALIS 296

Query: 665  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 507
            ADLD GEI RAKFDFDVVGHY+RP+VL+LIVRDH  +PV+FTS ++K E S K
Sbjct: 297  ADLDLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349


>sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4B;
            Short=TNIT4B; AltName: Full=Cyanoalanine nitrilase B;
            AltName: Full=Nitrilase 4B gi|1181615|dbj|BAA11770.1|
            nitrilase [Nicotiana tabacum]
          Length = 348

 Score =  593 bits (1530), Expect = e-167
 Identities = 284/353 (80%), Positives = 320/353 (90%)
 Frame = -2

Query: 1565 MALVPIPLIDNNSAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1386
            MALVP P+++      P+ AEVDMG +S+  TVRATVVQASTIFYDTPATL KAERL+++
Sbjct: 1    MALVPTPVVNEG----PMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAE 56

Query: 1385 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1206
            AA YG+QLVVFPEAFIGGYPRGS FG+++G+RT KGKEEFRKYHASAIDVPGPEV+ L A
Sbjct: 57   AASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAA 116

Query: 1205 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGGGSTIP 1026
            MAGKYKVYLVMGVIER+GYTLYCT+LFFDSQGHYLGKHRK+MPT+LER+IWGFG GSTIP
Sbjct: 117  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIP 176

Query: 1025 VFETAIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 846
            V++T +GKIG  ICWEN+MPLLRTAMY KGIEIYCAPTAD+RD+WQASMTHIALEGGCFV
Sbjct: 177  VYDTPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFV 236

Query: 845  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTVLAGPNYEGEALIS 666
            LSANQFCRRKDYPPPPEY FSGT EDL+P+S+VCAGGSVIISPSG VLAGPNYEGEALIS
Sbjct: 237  LSANQFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALIS 295

Query: 665  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 507
            ADLD GEI RAKFDFDVVGHY+RP+VL+LIVRDH  +PV+FTS ++K E S K
Sbjct: 296  ADLDLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348


>ref|XP_002328237.1| nitrilase 1 [Populus trichocarpa] gi|222837752|gb|EEE76117.1|
            nitrilase 1 [Populus trichocarpa]
          Length = 348

 Score =  593 bits (1530), Expect = e-167
 Identities = 285/353 (80%), Positives = 315/353 (89%)
 Frame = -2

Query: 1565 MALVPIPLIDNNSAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1386
            MALVP P      +  P+ AEVDM SDS+A TVRATV+QAST+FYDTPATL KAER +++
Sbjct: 1    MALVPAP-----PSETPLFAEVDMCSDSSATTVRATVIQASTVFYDTPATLDKAERFLAE 55

Query: 1385 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1206
            AA YGSQLVVFPEAFIGGYPRGS+FG T+GSRT KG+E+FRKYHASAIDVPGPEV+ L A
Sbjct: 56   AAGYGSQLVVFPEAFIGGYPRGSSFGATIGSRTAKGREDFRKYHASAIDVPGPEVDRLAA 115

Query: 1205 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGGGSTIP 1026
            MAGKYKVYLVMGVIEREGYTLYCT+LFFDSQGHY+GKHRK+MPT++ER++WGFG GSTIP
Sbjct: 116  MAGKYKVYLVMGVIEREGYTLYCTVLFFDSQGHYMGKHRKVMPTAVERIVWGFGDGSTIP 175

Query: 1025 VFETAIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 846
            VF T IGKIG  ICWEN+MPLLRTAMYGKGIEIYCAPTAD+RD WQA+MTHIALEGGCFV
Sbjct: 176  VFGTPIGKIGAAICWENRMPLLRTAMYGKGIEIYCAPTADSRDTWQATMTHIALEGGCFV 235

Query: 845  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTVLAGPNYEGEALIS 666
            LSANQFCRRKDYPPPPEY F G +EDL+P+SVVCAGGSVIISP GTVLAGPNY+GEALIS
Sbjct: 236  LSANQFCRRKDYPPPPEYVFLGVEEDLTPDSVVCAGGSVIISPLGTVLAGPNYDGEALIS 295

Query: 665  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 507
            ADLD GEI RAKFDFDVVGHYSRP+VL+L VRDHPT  V FTS +AKTE S K
Sbjct: 296  ADLDLGEIARAKFDFDVVGHYSRPEVLSLTVRDHPTNAVMFTSESAKTEASRK 348


>ref|XP_004139500.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A-like
            [Cucumis sativus]
          Length = 350

 Score =  592 bits (1526), Expect = e-166
 Identities = 283/353 (80%), Positives = 319/353 (90%)
 Frame = -2

Query: 1565 MALVPIPLIDNNSAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1386
            MALVP     ++     +IAEVDMGSDS+ + VRATVVQASTIFYDTPATL KAERL+++
Sbjct: 1    MALVPTTAPTDSQ----LIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAE 56

Query: 1385 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1206
            AA +GSQLVVFPEAF+GGYPRGSNFG+++G+RT KGKEEFRKYHASAIDVPGPEV+ L A
Sbjct: 57   AAGFGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAA 116

Query: 1205 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGGGSTIP 1026
            MAGKY+V+LVMGVIER+GYTLYCT+LFFD QG YLGKHRK+MPT+LER+IWGFG GSTIP
Sbjct: 117  MAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIP 176

Query: 1025 VFETAIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 846
            VFET+IGKIG  ICWEN+MPLLRTAMY KGIEIYCAPTAD+RD WQASMTHIALEGGCFV
Sbjct: 177  VFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFV 236

Query: 845  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTVLAGPNYEGEALIS 666
            LSANQFCRRKDYPPPPEY FSGT+E+L+P+SVVCAGGS IISPSGT+LAGPNY+GEALIS
Sbjct: 237  LSANQFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALIS 296

Query: 665  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 507
            ADLD GEI RAKFDFDVVGHY+RP+VL+L+VRDHPTTPV FTS + K E S K
Sbjct: 297  ADLDLGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCK 349