BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00009324
         (1846 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002877272.1| pectinesterase family protein [Arabidopsis l...   657   0.0  
gb|AER38245.1| PME4 [Gossypium barbadense]                            655   0.0  
ref|XP_004146188.1| PREDICTED: pectinesterase/pectinesterase inh...   649   0.0  
dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis]              649   0.0  
sp|Q43062.1|PME_PRUPE RecName: Full=Pectinesterase/pectinesteras...   648   0.0  

>ref|XP_002877272.1| pectinesterase family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297323110|gb|EFH53531.1| pectinesterase family protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 527

 Score =  657 bits (1696), Expect = 0.0
 Identities = 322/523 (61%), Positives = 391/523 (74%), Gaps = 21/523 (4%)
 Frame = +3

Query: 117  LMSNFSVMIFFFFTLSPFFVKSDYYQRT-----ELLKVPSATFLTSVKSTIDEVKVATSI 281
            L    S+  FF  T+S      + +  T     E LKVP   F  + K+ +D ++ A +I
Sbjct: 5    LQMGSSIFFFFLITVSLCSAHKEAFSSTGLVQMECLKVPPLEFAQAAKTVVDAIQKAVAI 64

Query: 282  VSKFVNKFVNSRLLNAINDCVDLLDYTADELSLTLSAIENPNGNDNSTGDRGVDLKAWLS 461
            VSKF  K   SR+ NAI DCVDLLD  A+ELS  +SA +NPNG DNSTGD G DL+ W+S
Sbjct: 65   VSKFDKKVGKSRVSNAILDCVDLLDSAAEELSWIISASQNPNGKDNSTGDVGSDLRTWIS 124

Query: 462  AALGNQETCLDGFEGTNSFVKKVIGGSLQKVTSRVANVLGMVGQVPSNKNSPRD------ 623
            AAL NQ+TCLDGFEGTN  +KK++ G L +V + V N+L MV   PS K  P+       
Sbjct: 125  AALSNQDTCLDGFEGTNGIIKKIVAGGLSRVGTTVRNLLTMVHSPPS-KAKPKPIKAHTM 183

Query: 624  ---------FPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGSFSSVIDAILAAPDYSTRR 776
                     FP+W+KPGDR+LLQ              DG+G+F+++ DA+LAAPDYST+R
Sbjct: 184  TKAHSGFSKFPSWVKPGDRKLLQTDNITVADAVVAT-DGTGNFTTISDAVLAAPDYSTKR 242

Query: 777  YVIYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFVDGWTTFRSATFAVSAKGF 956
            YVI+VKRGVY+ENV+IKKKKWN+M++GDG+DAT+I+GNR+F+DGWTTFRSATFAVS +GF
Sbjct: 243  YVIHVKRGVYEENVEIKKKKWNIMIVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGF 302

Query: 957  IARDISFENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTLYAHSLRQFYRECRITGTI 1136
            I RDI+F+NTAGPEKHQAVA+RSD+DL  FYRC  RGYQDTLYAHS+RQF+REC ITGT+
Sbjct: 303  IGRDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTV 362

Query: 1137 DYIFGNAAAVFQNCQILAKMGLPNQKNTITAQGRKDPNQSTGFSIQFCNISADSDLLPFV 1316
            D+IFG+A AVFQNCQI AK GLPNQKN+ITAQGRKDPN+ TGF+IQF NI+AD+DLLP  
Sbjct: 363  DFIFGDATAVFQNCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLPNS 422

Query: 1317 NTTQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFALSTLYYGEYMNYGPGTGLG 1496
            NTT TYLGRPWK YSRTVFMQ+YMS  + P GWLEW G+FAL TLYYGEYMN GPG  L 
Sbjct: 423  NTTATYLGRPWKLYSRTVFMQNYMSDAINPEGWLEWNGNFALDTLYYGEYMNSGPGASLD 482

Query: 1497 SRVKWPGYHQIDS-TQAATFTVTQFIDGNLWLPPTGVKFTAGL 1622
             RVKWPGYH +++  +A  FTV+QFI GNLWLP TG+ F AGL
Sbjct: 483  RRVKWPGYHVLNTPAEANNFTVSQFIQGNLWLPSTGITFIAGL 525


>gb|AER38245.1| PME4 [Gossypium barbadense]
          Length = 525

 Score =  655 bits (1691), Expect = 0.0
 Identities = 324/523 (61%), Positives = 394/523 (75%), Gaps = 23/523 (4%)
 Frame = +3

Query: 129  FSVMIFFFFTL-----SPFFVKSDYYQRTELLKVPSATFLTSVKSTIDEVKVATSIVSKF 293
            F +++FF   L     S     ++ +  TE LKVP+  F+ S+K+TID ++ ATS+VS+F
Sbjct: 5    FHLLVFFLVALCLCTNSNASSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQF 64

Query: 294  VNKFVNSRLLNAINDCVDLLDYTADELSLTLSAIENPNGNDNSTGDRGVDLKAWLSAALG 473
               F + RL NAI+DC+DLLD +ADELS T+SA +NPN  DNSTGD   DL+ WLSAA+ 
Sbjct: 65   GGFFHDFRLSNAISDCLDLLDSSADELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMV 124

Query: 474  NQETCLDGFEGTNSFVKKVIGGSLQKVTSRVANVLGMVGQVPSNK-NSPRD--------- 623
            NQ+TC+DGFEGTNS VK V+ GSL ++TS V N+L MV   P++K N  R+         
Sbjct: 125  NQQTCIDGFEGTNSMVKTVVSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGG 184

Query: 624  -------FPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGSFSSVIDAILAAPDYSTRRYV 782
                   FP W K  DRRLLQ              DGSG+F+ ++DA+  APD S  RYV
Sbjct: 185  GHPGQSRFPVWFKREDRRLLQI--NGVTANVVVAADGSGNFTRIMDAVETAPDKSMNRYV 242

Query: 783  IYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFVDGWTTFRSATFAVSAKGFIA 962
            IY+K+G+YKENV+IKKKKWNL+MIGDGMD T+ISGNR+F+DGWTT RSATFAVS +GFIA
Sbjct: 243  IYIKKGLYKENVEIKKKKWNLVMIGDGMDVTVISGNRSFIDGWTTLRSATFAVSGRGFIA 302

Query: 963  RDISFENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTLYAHSLRQFYRECRITGTIDY 1142
            RDI+FENTAGP+KHQAVA+RSDSDLS F+RC  +GYQD+LY H++RQFYREC+ITGT+D+
Sbjct: 303  RDITFENTAGPQKHQAVALRSDSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDF 362

Query: 1143 IFGNAAAVFQNCQILAKMGLPNQKNTITAQGRKDPNQSTGFSIQFCNISADSDLLPFVNT 1322
            IFG+ A +FQNCQILAK GLP+QKNTITAQGRKDPNQ TGFSIQFCNISAD+DLLP VN+
Sbjct: 363  IFGDGAVLFQNCQILAKQGLPSQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNS 422

Query: 1323 TQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFALSTLYYGEYMNYGPGTGLGSR 1502
            T TYLGRPWK YSRT+ MQSY+S  + P GWLEW  DFAL TLYY EYMN GPG  L  R
Sbjct: 423  TPTYLGRPWKLYSRTIIMQSYISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSER 482

Query: 1503 VKWPGYHQI-DSTQAATFTVTQFIDGNLWLPPTGVKFTAGLTV 1628
            VKWPGYH + +S QA  FTV QFI+G+LWLP TGVK+T+G  V
Sbjct: 483  VKWPGYHVLNNSAQAVNFTVAQFIEGDLWLPSTGVKYTSGFGV 525


>ref|XP_004146188.1| PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like
            [Cucumis sativus]
          Length = 507

 Score =  649 bits (1675), Expect = 0.0
 Identities = 317/497 (63%), Positives = 385/497 (77%), Gaps = 1/497 (0%)
 Frame = +3

Query: 135  VMIFFFFTLSPFFVKSDYYQRTELLKVPSATFLTSVKSTIDEVKVATSIVSKFVNKFVNS 314
            ++I  F  L P     D  Q TE L VPS+ F  S+ STID V+   +I S F     + 
Sbjct: 14   LLILLFPMLLPASSPPDLVQ-TECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDF 72

Query: 315  RLLNAINDCVDLLDYTADELSLTLSAIENPNGNDNSTGDRGVDLKAWLSAALGNQETCLD 494
            RL  AI+DC+DLLD +AD+LS +LSA +NP   ++STGD   DLK WLSAA+ N ETC+D
Sbjct: 73   RLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMD 132

Query: 495  GFEGTNSFVKKVIGGSLQKVTSRVANVLGMVGQVPSNKNSPRDFPAWMKPGDRRLLQAPT 674
            GFEGTNS +K ++ G + ++TS++ ++L MV  +P   N P +FP+W+K  D+ LLQ   
Sbjct: 133  GFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIP---NQPSEFPSWLKSEDQNLLQI-- 187

Query: 675  XXXXXXXXXXMDGSGSFSSVIDAILAAPDYSTRRYVIYVKRGVYKENVDIKKKKWNLMMI 854
                       DG+G F++V+DA+LAAPD S RRYVIY+K+GVY ENV+IKKKKWNLMMI
Sbjct: 188  NDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMI 247

Query: 855  GDGMDATIISGNRNFVDGWTTFRSATFAVSAKGFIARDISFENTAGPEKHQAVAVRSDSD 1034
            GDG+DATIISGNR+F+DGWTTFRSATFAVS +GFIARDI+FENTAG EKHQAVA+RSDSD
Sbjct: 248  GDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSD 307

Query: 1035 LSAFYRCGFRGYQDTLYAHSLRQFYRECRITGTIDYIFGNAAAVFQNCQILAKMGLPNQK 1214
            LS F+RC  RGYQDTLY H++RQFYREC+I+GT+D++FG+A  VFQNC ILAK GLPNQK
Sbjct: 308  LSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQK 367

Query: 1215 NTITAQGRKDPNQSTGFSIQFCNISADSDLLPFVNTTQTYLGRPWKQYSRTVFMQSYMSS 1394
            NTITAQGRKDPNQ TGFSIQFCNISADSDL P VNTT TYLGRPWK+YSRT+ MQSY+S 
Sbjct: 368  NTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISD 427

Query: 1395 VLMPAGWLEWQGDFALSTLYYGEYMNYGPGTGLGSRVKWPGYHQIDSTQAAT-FTVTQFI 1571
             + P GWLEW  +FAL+TL+Y E+MNYGPG GL  RV WPGYH+++ T  AT FTV QFI
Sbjct: 428  AIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFI 487

Query: 1572 DGNLWLPPTGVKFTAGL 1622
            +GNLWLP TGVK+TAGL
Sbjct: 488  EGNLWLPSTGVKYTAGL 504


>dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis]
          Length = 509

 Score =  649 bits (1673), Expect = 0.0
 Identities = 315/477 (66%), Positives = 377/477 (79%), Gaps = 1/477 (0%)
 Frame = +3

Query: 195  RTELLKVPSATFLTSVKSTIDEVKVATSIVSKFVNKFVNSRLLNAINDCVDLLDYTADEL 374
            R+E LKVP++ F  S++ TI  V+   SI+S+F   F +  L NAI+DC+DLLD+TADEL
Sbjct: 33   RSECLKVPASEFAGSLRDTIGAVQQVASILSQFATAFGDFHLSNAISDCLDLLDFTADEL 92

Query: 375  SLTLSAIENPNGNDNSTGDRGVDLKAWLSAALGNQETCLDGFEGTNSFVKKVIGGSLQKV 554
            + +LSA +NP G DNSTG    DL+ WLSAAL NQ+TC DGFEGTNS VK ++   L +V
Sbjct: 93   NWSLSASQNPEGKDNSTGKLSSDLRTWLSAALVNQDTCSDGFEGTNSIVKGLVTTGLNQV 152

Query: 555  TSRVANVLGMVGQVPSNKNSPRDFPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGSFSSV 734
            TS V  +L  V     +     + P+W+K  DR+LLQA             DG+G+F++V
Sbjct: 153  TSLVQGLLTQVQPNTDHHGPNGEIPSWVKAEDRKLLQA--GGVNVDAVVAQDGTGNFTNV 210

Query: 735  IDAILAAPDYSTRRYVIYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFVDGWT 914
             DA+LAAPD S  RYVIY+K G YKENV+IKKKKWNLMMIGDGMDATIISG+RNFVDGWT
Sbjct: 211  TDAVLAAPDESMTRYVIYIKGGTYKENVEIKKKKWNLMMIGDGMDATIISGSRNFVDGWT 270

Query: 915  TFRSATFAVSAKGFIARDISFENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTLYAHS 1094
            TFRSATFAVS +GFIARD++FENTAGPEKHQAVA+RSDSDLS FYRC  RGYQDTLYAH+
Sbjct: 271  TFRSATFAVSGRGFIARDLTFENTAGPEKHQAVALRSDSDLSVFYRCQIRGYQDTLYAHA 330

Query: 1095 LRQFYRECRITGTIDYIFGNAAAVFQNCQILAKMGLPNQKNTITAQGRKDPNQSTGFSIQ 1274
            +RQFYR+C+I+GT+D+IFG+A  VFQNCQILAK GLPNQKN++TAQGRKDPN+ TG SIQ
Sbjct: 331  MRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSVTAQGRKDPNEPTGISIQ 390

Query: 1275 FCNISADSDLLPFVNTTQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFALSTLY 1454
            FCNI+AD+DLLPFVN+T TYLGRPWK YSRTV MQS++S+ + P GWLEW  DFALSTL 
Sbjct: 391  FCNITADADLLPFVNSTSTYLGRPWKLYSRTVIMQSFLSNAIRPEGWLEWNADFALSTLS 450

Query: 1455 YGEYMNYGPGTGLGSRVKWPGYHQI-DSTQAATFTVTQFIDGNLWLPPTGVKFTAGL 1622
            YGEY+NYGPG GL SRVKWPGY    +STQ   +TV QFI+G+LWLP TGVK+TAGL
Sbjct: 451  YGEYINYGPGAGLESRVKWPGYRVFNESTQVQNYTVAQFIEGDLWLPTTGVKYTAGL 507


>sp|Q43062.1|PME_PRUPE RecName: Full=Pectinesterase/pectinesterase inhibitor PPE8B;
            Includes: RecName: Full=Pectinesterase inhibitor PPE8B;
            AltName: Full=Pectin methylesterase inhibitor PPE8B;
            Includes: RecName: Full=Pectinesterase PPE8B; Short=PE
            PPE8B; AltName: Full=Pectin methylesterase PPE8B; Flags:
            Precursor gi|1213629|emb|CAA65237.1| pectinesterase
            [Prunus persica]
          Length = 522

 Score =  648 bits (1671), Expect = 0.0
 Identities = 317/476 (66%), Positives = 379/476 (79%), Gaps = 2/476 (0%)
 Frame = +3

Query: 195  RTELLKVPSATFLTSVKSTIDEVKVATSIVSKFVNKFVNSRLLNAINDCVDLLDYTADEL 374
            +T+ LKV +  F  S+K TID V+   SI+S+F N F + RL NAI+DC+DLLD++ADEL
Sbjct: 45   QTQCLKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADEL 104

Query: 375  SLTLSAIENPNGNDNSTGDRGVDLKAWLSAALGNQETCLDGFEGTNSFVKKVIGGSLQKV 554
            + +LSA +N  G +NSTG    DL+ WLSAAL NQ+TC +GFEGTNS V+ +I   L +V
Sbjct: 105  NWSLSASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQV 164

Query: 555  TSRVANVLGMVGQVPSNKNSPRDFPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGSFSSV 734
            TS V  +L  V    + +      P+W+K  DR+LLQA             DG+G+F++V
Sbjct: 165  TSLVQELLTQVHPNSNQQGPNGQIPSWVKTKDRKLLQAD--GVSVDAIVAQDGTGNFTNV 222

Query: 735  IDAILAAPDYSTRRYVIYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFVDGWT 914
             DA+LAAPDYS RRYVIY+KRG YKENV+IKKKKWNLMMIGDGMDATIISGNR+FVDGWT
Sbjct: 223  TDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWT 282

Query: 915  TFRSATFAVSAKGFIARDISFENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTLYAHS 1094
            TFRSATFAVS +GFIARDI+FENTAGPEKHQAVA+RSDSDLS FYRC  RGYQDTLY H+
Sbjct: 283  TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHT 342

Query: 1095 LRQFYRECRITGTIDYIFGNAAAVFQNCQILAKMGLPNQKNTITAQGRKDPNQSTGFSIQ 1274
            +RQFYR+C+I+GT+D+IFG+A  VFQNCQILAK GLPNQKN+ITAQGRKDPN+ TG SIQ
Sbjct: 343  MRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQ 402

Query: 1275 FCNISADSDL-LPFVNTTQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFALSTL 1451
            FCNI+ADSDL    VN+T TYLGRPWK YSRTV MQS++S+V+ P GWLEW GDFAL++L
Sbjct: 403  FCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSL 462

Query: 1452 YYGEYMNYGPGTGLGSRVKWPGYHQI-DSTQAATFTVTQFIDGNLWLPPTGVKFTA 1616
            +YGEYMNYGPG GLGSRVKWPGY    +STQA  +TV QFI+GNLWLP TGVK+TA
Sbjct: 463  FYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTA 518