BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00009255
         (1356 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268333.1| PREDICTED: magnesium transporter MRS2-3-like...   288   e-117
emb|CBI22425.3| unnamed protein product [Vitis vinifera]              288   e-117
ref|XP_004136933.1| PREDICTED: magnesium transporter MRS2-3-like...   239   e-104
ref|XP_002313918.1| magnesium transporter [Populus trichocarpa] ...   246   e-104
ref|XP_003610633.1| Magnesium transporter [Medicago truncatula] ...   211   2e-94

>ref|XP_002268333.1| PREDICTED: magnesium transporter MRS2-3-like isoform 2 [Vitis
            vinifera]
          Length = 502

 Score =  288 bits (736), Expect(2) = e-117
 Identities = 162/244 (66%), Positives = 181/244 (74%), Gaps = 7/244 (2%)
 Frame = -2

Query: 1355 EERPVRPDDSPG-FPQCQSPQGGVKLDGK-----NDGPKVLPFEFVALEACIEAACSCLE 1194
            + R V P +  G F Q Q    G K DG+      DG KVLPFEF+ALEAC+EAACSCLE
Sbjct: 166  QSRVVSPQNFSGDFSQFQDQDEGAKADGRPGLENQDGLKVLPFEFIALEACLEAACSCLE 225

Query: 1193 SEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXXXXXXXXXXXDM 1014
            +EARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR           DM
Sbjct: 226  NEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEHLLDDDEDM 285

Query: 1013 AEMYLTDKMLLQRLDEXXXXXXXLNGRDGMDDGVIQSDIEDRICLENPLEANGVPSNYEG 834
            AEMYLTDKM+ Q L+        ++ RDGMDDGV+ +D++D I  E  LEANGV + YEG
Sbjct: 286  AEMYLTDKMIQQHLEN--SSVSSIHERDGMDDGVLHADMDDGIPDEISLEANGVSATYEG 343

Query: 833  DLRNIIN-RDHSLGVPNGLSRDSHGTLTSTTRSVIGKHLDVEELEMLLEAYFVQIDGTLN 657
            DL+NI N ++H  G P+ L RDSHGT TSTT S I KHLDVEELEMLLEAYFVQIDGTLN
Sbjct: 344  DLQNIDNPQEHLFGTPHALGRDSHGTHTSTTHSAISKHLDVEELEMLLEAYFVQIDGTLN 403

Query: 656  KLST 645
            KLST
Sbjct: 404  KLST 407



 Score =  160 bits (406), Expect(2) = e-117
 Identities = 79/95 (83%), Positives = 85/95 (89%), Gaps = 3/95 (3%)
 Frame = -3

Query: 598 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSGFVVVAGIFGMNIKIDLY---K 428
           LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVS FVVVAGIFGMNI I+L+   K
Sbjct: 408 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIHIELFDDKK 467

Query: 427 KGMPQFFWTVGGGITGTIFLYVFAIAWCKHKRLLE 323
            G+ +F WTVGGG TG+IFLYV AIAWC+HKRLLE
Sbjct: 468 SGVQEFLWTVGGGATGSIFLYVIAIAWCRHKRLLE 502


>emb|CBI22425.3| unnamed protein product [Vitis vinifera]
          Length = 435

 Score =  288 bits (736), Expect(2) = e-117
 Identities = 162/244 (66%), Positives = 181/244 (74%), Gaps = 7/244 (2%)
 Frame = -2

Query: 1355 EERPVRPDDSPG-FPQCQSPQGGVKLDGK-----NDGPKVLPFEFVALEACIEAACSCLE 1194
            + R V P +  G F Q Q    G K DG+      DG KVLPFEF+ALEAC+EAACSCLE
Sbjct: 99   QSRVVSPQNFSGDFSQFQDQDEGAKADGRPGLENQDGLKVLPFEFIALEACLEAACSCLE 158

Query: 1193 SEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXXXXXXXXXXXDM 1014
            +EARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR           DM
Sbjct: 159  NEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEHLLDDDEDM 218

Query: 1013 AEMYLTDKMLLQRLDEXXXXXXXLNGRDGMDDGVIQSDIEDRICLENPLEANGVPSNYEG 834
            AEMYLTDKM+ Q L+        ++ RDGMDDGV+ +D++D I  E  LEANGV + YEG
Sbjct: 219  AEMYLTDKMIQQHLEN--SSVSSIHERDGMDDGVLHADMDDGIPDEISLEANGVSATYEG 276

Query: 833  DLRNIIN-RDHSLGVPNGLSRDSHGTLTSTTRSVIGKHLDVEELEMLLEAYFVQIDGTLN 657
            DL+NI N ++H  G P+ L RDSHGT TSTT S I KHLDVEELEMLLEAYFVQIDGTLN
Sbjct: 277  DLQNIDNPQEHLFGTPHALGRDSHGTHTSTTHSAISKHLDVEELEMLLEAYFVQIDGTLN 336

Query: 656  KLST 645
            KLST
Sbjct: 337  KLST 340



 Score =  160 bits (406), Expect(2) = e-117
 Identities = 79/95 (83%), Positives = 85/95 (89%), Gaps = 3/95 (3%)
 Frame = -3

Query: 598 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSGFVVVAGIFGMNIKIDLY---K 428
           LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVS FVVVAGIFGMNI I+L+   K
Sbjct: 341 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIHIELFDDKK 400

Query: 427 KGMPQFFWTVGGGITGTIFLYVFAIAWCKHKRLLE 323
            G+ +F WTVGGG TG+IFLYV AIAWC+HKRLLE
Sbjct: 401 SGVQEFLWTVGGGATGSIFLYVIAIAWCRHKRLLE 435


>ref|XP_004136933.1| PREDICTED: magnesium transporter MRS2-3-like [Cucumis sativus]
            gi|449495702|ref|XP_004159919.1| PREDICTED: magnesium
            transporter MRS2-3-like [Cucumis sativus]
          Length = 494

 Score =  239 bits (610), Expect(2) = e-104
 Identities = 139/238 (58%), Positives = 162/238 (68%), Gaps = 3/238 (1%)
 Frame = -2

Query: 1349 RPVRPDDSPGFPQCQSPQGGVKLDG---KNDGPKVLPFEFVALEACIEAACSCLESEART 1179
            R   P    GFPQ +  +G   +       +G KVLPFEFVALEAC+EAACSCLESEA T
Sbjct: 167  RTQSPPSYQGFPQAEEEEGKESMKQGLENREGLKVLPFEFVALEACLEAACSCLESEADT 226

Query: 1178 LEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXXXXXXXXXXXDMAEMYL 999
            LE EAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR           DMAEMYL
Sbjct: 227  LELEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEHLLDDDEDMAEMYL 286

Query: 998  TDKMLLQRLDEXXXXXXXLNGRDGMDDGVIQSDIEDRICLENPLEANGVPSNYEGDLRNI 819
            T+KM+ Q+L+           RD M++   Q   +D    E  ++  G+ +NY+G++   
Sbjct: 287  TEKMVQQQLENSSTSSIPE--RDDMEEDDQQLGKDDSSPTEISMDGGGISANYDGNMD-- 342

Query: 818  INRDHSLGVPNGLSRDSHGTLTSTTRSVIGKHLDVEELEMLLEAYFVQIDGTLNKLST 645
             ++DH  G  + + RDSHGT TSTT S I KHLDVEELEMLLEAYFVQIDGTLNKLST
Sbjct: 343  ASQDHLFGASH-VGRDSHGTRTSTTHSAISKHLDVEELEMLLEAYFVQIDGTLNKLST 399



 Score =  167 bits (424), Expect(2) = e-104
 Identities = 83/95 (87%), Positives = 86/95 (90%), Gaps = 3/95 (3%)
 Frame = -3

Query: 598 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSGFVVVAGIFGMNIKIDLY---K 428
           LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVS FVVVAGIFGMNI I+L+   K
Sbjct: 400 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIHIELFESEK 459

Query: 427 KGMPQFFWTVGGGITGTIFLYVFAIAWCKHKRLLE 323
            GMP+F WTVGGG TGTIFLYV AIAWCKHKRLLE
Sbjct: 460 AGMPEFLWTVGGGATGTIFLYVIAIAWCKHKRLLE 494


>ref|XP_002313918.1| magnesium transporter [Populus trichocarpa]
            gi|222850326|gb|EEE87873.1| magnesium transporter
            [Populus trichocarpa]
          Length = 462

 Score =  246 bits (628), Expect(2) = e-104
 Identities = 147/238 (61%), Positives = 169/238 (71%), Gaps = 7/238 (2%)
 Frame = -2

Query: 1337 PDDSP-GFPQCQSPQGGVKLDGKN-----DGPKVLPFEFVALEACIEAACSCLESEARTL 1176
            P+ SP GF   +    G+K +GK      DG KVLPFEFVALEAC+EAACSCLESEA+TL
Sbjct: 135  PEKSPVGFSHFEDCNEGIKAEGKQGFENRDGSKVLPFEFVALEACLEAACSCLESEAKTL 194

Query: 1175 EQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXXXXXXXXXXXDMAEMYLT 996
            EQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR           DM E+YLT
Sbjct: 195  EQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDEIEHLLDDDEDMVELYLT 254

Query: 995  DKMLLQRLDEXXXXXXXLNGRDGMDDGVIQSDIEDRICLENPLEANGVPSNYEGDLRNII 816
            +K++ Q+L++       LN  + MDD  +Q+D +D I  E  L   G  ++++ D  NI 
Sbjct: 255  EKLVQQQLED--SSTSSLNEGNDMDDDELQADSDDSIPAEVSL---GALASHKDDFNNID 309

Query: 815  N-RDHSLGVPNGLSRDSHGTLTSTTRSVIGKHLDVEELEMLLEAYFVQIDGTLNKLST 645
            N  DH    PNGL R SH T TSTTRS I KHL+VEELEMLLEAYFVQIDGTLNKLST
Sbjct: 310  NEHDHLFTAPNGLGRYSH-THTSTTRSAISKHLNVEELEMLLEAYFVQIDGTLNKLST 366



 Score =  159 bits (401), Expect(2) = e-104
 Identities = 79/96 (82%), Positives = 84/96 (87%), Gaps = 4/96 (4%)
 Frame = -3

Query: 598 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSGFVVVAGIFGMNIKIDLYKK-- 425
           LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVS FVVV GIFGMNIKI L+K+  
Sbjct: 367 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSCFVVVVGIFGMNIKIALFKEDV 426

Query: 424 --GMPQFFWTVGGGITGTIFLYVFAIAWCKHKRLLE 323
             GMP+F WT+ GG +G IFLYV AIAWCKHKRLLE
Sbjct: 427 QTGMPKFLWTLAGGTSGVIFLYVIAIAWCKHKRLLE 462


>ref|XP_003610633.1| Magnesium transporter [Medicago truncatula]
            gi|355511968|gb|AES93591.1| Magnesium transporter
            [Medicago truncatula]
          Length = 444

 Score =  211 bits (537), Expect(2) = 2e-94
 Identities = 131/215 (60%), Positives = 147/215 (68%), Gaps = 3/215 (1%)
 Frame = -2

Query: 1280 DGKNDGPKVLPFEFVALEACIEAACSCLESEARTLEQEAHPALDKLTSKISTLNLERVRQ 1101
            +  + G K+LPFEFVALEAC+EAACS LESEA+TLEQEAHPALDKLTSKISTLNLERVRQ
Sbjct: 139  EDSHGGIKILPFEFVALEACLEAACSVLESEAKTLEQEAHPALDKLTSKISTLNLERVRQ 198

Query: 1100 IKSRLVAITGRVQKVRXXXXXXXXXXXDMAEMYLTDKMLLQRLDEXXXXXXXLNGRDGMD 921
            IKSRLVAITGRVQKVR           DMAEMYLT+K+L Q+L++             +D
Sbjct: 199  IKSRLVAITGRVQKVRDELEHLLDDDEDMAEMYLTEKLLQQQLEQTSDEDTVETPDHDVD 258

Query: 920  DGVIQSDIEDRI-CLENPLEAN-GVPSNY-EGDLRNIINRDHSLGVPNGLSRDSHGTLTS 750
            D  +  DI   +   E  LEA  G   NY E D  N+ +R+   G  N   RDS GT  S
Sbjct: 259  DNGV--DINQSVPAAEISLEAGVGGGVNYVEEDEDNLNSREQMFGASN--LRDSRGTRAS 314

Query: 749  TTRSVIGKHLDVEELEMLLEAYFVQIDGTLNKLST 645
            TT SV    LDVEELEMLLEAYFVQIDGTLNKLST
Sbjct: 315  TTYSVTTTKLDVEELEMLLEAYFVQIDGTLNKLST 349



 Score =  162 bits (410), Expect(2) = 2e-94
 Identities = 81/95 (85%), Positives = 84/95 (88%), Gaps = 3/95 (3%)
 Frame = -3

Query: 598 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSGFVVVAGIFGMNIKIDLYKK-- 425
           LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVS FVVVAGIFGMNI I+L+ K  
Sbjct: 350 LREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNINIELFDKNL 409

Query: 424 -GMPQFFWTVGGGITGTIFLYVFAIAWCKHKRLLE 323
            GM +F WTVGGG  GTIFLYV AIAWCKHKRLLE
Sbjct: 410 YGMREFMWTVGGGTAGTIFLYVVAIAWCKHKRLLE 444