BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00009156
         (1565 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]             474   e-131
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   466   e-129
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              466   e-129
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso...   451   e-124
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]                  444   e-122

>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
          Length = 784

 Score =  474 bits (1220), Expect = e-131
 Identities = 250/468 (53%), Positives = 310/468 (66%), Gaps = 4/468 (0%)
 Frame = -2

Query: 1564 FFTSAGGDLKGIRTLLELHKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIHDDNCQK 1385
            F +S GG +K I ++LEL + SQI+I P+E VLE  N WTS  L Q LSN  IH D   K
Sbjct: 347  FSSSLGGYMKDIGSVLELFRASQIIIHPDEFVLEKQNFWTSQFLIQELSNGSIHADGLNK 406

Query: 1384 ----EVVNALKFTYDADIERLENRRNIEYYNVDSLRILKASYRCSNVDNRDIIQFGVEDY 1217
                EV +ALKF Y A +ERL NRR IE YN ++ R+LK +Y  SN+ N D +   VED+
Sbjct: 407  YVSQEVDDALKFPYHASLERLSNRRAIENYNKNNTRVLKTAYSSSNIGNEDFLNLAVEDF 466

Query: 1216 NFCQGILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDARMSWTKNS 1037
            N CQ I ++EL  L RW+ ENRLDKLKFAR KL Y YFS A  +F+PELSDAR+SW KN 
Sbjct: 467  NICQSIQREELKDLARWITENRLDKLKFARQKLAYCYFSAAATLFSPELSDARISWAKNG 526

Query: 1036 FLSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQALHKTVNDLGDIA 857
             L+TVVDDFFDV GS EELVNLI LVEKW+ + +T+ CSE VEIIF ALH T+ D  D  
Sbjct: 527  VLTTVVDDFFDVGGSVEELVNLIQLVEKWDVDVSTDCCSENVEIIFSALHSTICDFADKG 586

Query: 856  FTRQGRSVRSHLIDIWLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGPVVLITQY 677
             T QGR+V SH+IDIWL LLKSM+KE EW RDKS P M+EYM N  +SFALGP+VL   Y
Sbjct: 587  LTLQGRNVISHIIDIWLNLLKSMLKEAEWLRDKSVPSMDEYMTNGYVSFALGPIVLPALY 646

Query: 676  FLEPTLSKKVITSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEYNKLFKHMNICCR 497
             + P LS++++ +P                                E + L++ M+ C R
Sbjct: 647  CVGPKLSEEIVGTP--------------------------------ELHHLYEIMSTCGR 674

Query: 496  LLNDIQSFKREGEEGKLNSVLLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKG 317
            LLNDIQ+FKRE EEGKLN+V LC IH      ++E ++E+K  I   RRELL LVL  KG
Sbjct: 675  LLNDIQTFKRESEEGKLNAVSLCMIHGGGDCTKEETIKELKSFIAGKRRELLKLVLQEKG 734

Query: 316  SIVPKGCKDLFWKTFRVTHLFYREKDGFSSSLEMVNAVNAVIHKPLSL 173
            S+VP+ CKDLFWK  +V HLFY + DGF+S  EM N+VNAV+ +P+ L
Sbjct: 735  SVVPRACKDLFWKMIKVLHLFYMKDDGFTSH-EMFNSVNAVLEEPIVL 781


>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
            vinifera]
          Length = 793

 Score =  466 bits (1200), Expect = e-129
 Identities = 240/463 (51%), Positives = 314/463 (67%), Gaps = 4/463 (0%)
 Frame = -2

Query: 1549 GGDLKGIRTLLELHKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIHDDNCQK----E 1382
            G D K +   LEL + S+++I P+E VLE  NSW+SH L+Q LSN  IH D   K    E
Sbjct: 361  GQDFKDLGAALELFRASEMIIHPDEVVLEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQE 420

Query: 1381 VVNALKFTYDADIERLENRRNIEYYNVDSLRILKASYRCSNVDNRDIIQFGVEDYNFCQG 1202
            V +AL+F Y A+++R+ NRR+IE+YNVD  RILK +YR S+V N+D ++  VED+NFCQ 
Sbjct: 421  VEDALRFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQS 480

Query: 1201 ILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDARMSWTKNSFLSTV 1022
            I Q EL  LERW+ ENRLDKLKFAR KL Y YFS A  +F+PE SDAR+SW KNS L+TV
Sbjct: 481  IHQNELKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTV 540

Query: 1021 VDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQALHKTVNDLGDIAFTRQG 842
            VDDFFD+ GS EEL+NLI LVEKW+ + A + CSE+VEI+F ALH T++++G  A   Q 
Sbjct: 541  VDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSALHSTISEIGVKASAWQA 600

Query: 841  RSVRSHLIDIWLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGPVVLITQYFLEPT 662
            R+V SH+IDIWL LL+SM++E +W  +KSAP M+EYM NA +SFALGP+VL   YF+ P 
Sbjct: 601  RNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEYMTNAYVSFALGPIVLPALYFVGPK 660

Query: 661  LSKKVITSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEYNKLFKHMNICCRLLNDI 482
            LS++V+  P                                E +KL+K M+ C RLLNDI
Sbjct: 661  LSEEVVEGP--------------------------------ECHKLYKLMSTCGRLLNDI 688

Query: 481  QSFKREGEEGKLNSVLLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKGSIVPK 302
             SFKRE +EGK N++ L  IH      E++A+RE+KG++   RREL  LVL  KGS VP+
Sbjct: 689  HSFKRESKEGKANALALHMIHGNGVTTEEQAIREMKGLVKSQRRELQRLVLQEKGSTVPR 748

Query: 301  GCKDLFWKTFRVTHLFYREKDGFSSSLEMVNAVNAVIHKPLSL 173
             CKDLFWK  +V H FY + DGF+S  +M+ AV +VI++P+ L
Sbjct: 749  ICKDLFWKMSKVLHTFYEKDDGFTSH-DMLRAVKSVIYEPVLL 790


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  466 bits (1200), Expect = e-129
 Identities = 240/463 (51%), Positives = 314/463 (67%), Gaps = 4/463 (0%)
 Frame = -2

Query: 1549 GGDLKGIRTLLELHKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIHDDNCQK----E 1382
            G D K +   LEL + S+++I P+E VLE  NSW+SH L+Q LSN  IH D   K    E
Sbjct: 394  GQDFKDLGAALELFRASEMIIHPDEVVLEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQE 453

Query: 1381 VVNALKFTYDADIERLENRRNIEYYNVDSLRILKASYRCSNVDNRDIIQFGVEDYNFCQG 1202
            V +AL+F Y A+++R+ NRR+IE+YNVD  RILK +YR S+V N+D ++  VED+NFCQ 
Sbjct: 454  VEDALRFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQS 513

Query: 1201 ILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDARMSWTKNSFLSTV 1022
            I Q EL  LERW+ ENRLDKLKFAR KL Y YFS A  +F+PE SDAR+SW KNS L+TV
Sbjct: 514  IHQNELKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTV 573

Query: 1021 VDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQALHKTVNDLGDIAFTRQG 842
            VDDFFD+ GS EEL+NLI LVEKW+ + A + CSE+VEI+F ALH T++++G  A   Q 
Sbjct: 574  VDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSALHSTISEIGVKASAWQA 633

Query: 841  RSVRSHLIDIWLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGPVVLITQYFLEPT 662
            R+V SH+IDIWL LL+SM++E +W  +KSAP M+EYM NA +SFALGP+VL   YF+ P 
Sbjct: 634  RNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEYMTNAYVSFALGPIVLPALYFVGPK 693

Query: 661  LSKKVITSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEYNKLFKHMNICCRLLNDI 482
            LS++V+  P                                E +KL+K M+ C RLLNDI
Sbjct: 694  LSEEVVEGP--------------------------------ECHKLYKLMSTCGRLLNDI 721

Query: 481  QSFKREGEEGKLNSVLLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKGSIVPK 302
             SFKRE +EGK N++ L  IH      E++A+RE+KG++   RREL  LVL  KGS VP+
Sbjct: 722  HSFKRESKEGKANALALHMIHGNGVTTEEQAIREMKGLVKSQRRELQRLVLQEKGSTVPR 781

Query: 301  GCKDLFWKTFRVTHLFYREKDGFSSSLEMVNAVNAVIHKPLSL 173
             CKDLFWK  +V H FY + DGF+S  +M+ AV +VI++P+ L
Sbjct: 782  ICKDLFWKMSKVLHTFYEKDDGFTSH-DMLRAVKSVIYEPVLL 823


>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 808

 Score =  451 bits (1161), Expect = e-124
 Identities = 241/467 (51%), Positives = 311/467 (66%), Gaps = 4/467 (0%)
 Frame = -2

Query: 1564 FFTSAGGDLKGIRTLLELHKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIHDDN--- 1394
            FF S GG LK  RT+LEL++ SQI+  P+EP+LE  NSWT+H L++ LS+   + D    
Sbjct: 302  FFDSLGGYLKDTRTVLELYRASQILY-PDEPLLEKQNSWTNHFLEKCLSSGSSYADGPRE 360

Query: 1393 CQKEVV-NALKFTYDADIERLENRRNIEYYNVDSLRILKASYRCSNVDNRDIIQFGVEDY 1217
            C  EVV NAL   Y AD+ERL NRR+IE YNVD  RILKASYRC N  N+  ++  VED+
Sbjct: 361  CITEVVHNALNCPYYADLERLTNRRSIENYNVDETRILKASYRCLNTGNQHFLKLAVEDF 420

Query: 1216 NFCQGILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDARMSWTKNS 1037
            N CQ I Q+EL  L RWV E RL+KLKFAR KL Y YFS A  +F PELSDAR+SW KN 
Sbjct: 421  NLCQLIHQEELQQLGRWVVEKRLNKLKFARQKLGYCYFSAAATLFAPELSDARLSWAKNG 480

Query: 1036 FLSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQALHKTVNDLGDIA 857
             L+TVVDDFFDV GS EEL+NLI L+EKW+ + +T++CSE+VEIIF AL  T++++GD A
Sbjct: 481  VLTTVVDDFFDVGGSVEELINLIQLIEKWDVDESTHFCSEQVEIIFSALRSTISEIGDKA 540

Query: 856  FTRQGRSVRSHLIDIWLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGPVVLITQY 677
            FT QGR V SH+I IWL LLKSM+ E  W + KS P ++EYM N  +SFALGP+VL   +
Sbjct: 541  FTWQGRKVTSHVIKIWLDLLKSMLTETLWTKSKSIPTLDEYMINGYVSFALGPIVLPALF 600

Query: 676  FLEPTLSKKVITSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEYNKLFKHMNICCR 497
             + P L+++ +  P                                E + LFK M  C R
Sbjct: 601  LVGPKLTEEDVRDP--------------------------------ELHDLFKAMGTCGR 628

Query: 496  LLNDIQSFKREGEEGKLNSVLLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKG 317
            LLND + F+RE +EGKLN+V L  I     +NE+EA+R++KG+I+  R ELL LVL  K 
Sbjct: 629  LLNDWRGFQRESKEGKLNAVSLHMIQGNGGVNEEEAIRKIKGLINSQRSELLRLVLREKN 688

Query: 316  SIVPKGCKDLFWKTFRVTHLFYREKDGFSSSLEMVNAVNAVIHKPLS 176
            S +P+ CKDLFWK  +V HLFY + DGF+S+ EM++  NAVI +P++
Sbjct: 689  SNIPRACKDLFWKMIKVLHLFYLKDDGFTSN-EMISTANAVITEPVA 734


>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
          Length = 737

 Score =  444 bits (1143), Expect = e-122
 Identities = 240/467 (51%), Positives = 299/467 (64%), Gaps = 4/467 (0%)
 Frame = -2

Query: 1561 FTSAGGDLKGIRTLLELHKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIHDDNCQK- 1385
            F S GG +K I   LEL + S+ +I P+E VLE  + WTSH LKQ LSN +I      K 
Sbjct: 302  FNSLGGYMKDIDDALELFRASETIIHPDESVLEKQHHWTSHFLKQELSNTLIRGHKLNKH 361

Query: 1384 ---EVVNALKFTYDADIERLENRRNIEYYNVDSLRILKASYRCSNVDNRDIIQFGVEDYN 1214
               EV +ALK    A + RL  R  I+YYN DS RILK+ YRC N+ N D ++  V+D N
Sbjct: 362  IGLEVDDALKLPRYAILGRLSTRTAIKYYNTDSTRILKSYYRCLNIGNEDFLKLAVDDLN 421

Query: 1213 FCQGILQKELASLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDARMSWTKNSF 1034
             CQ I ++EL  L RWV+E RLDKL FAR K  Y YFS A  +F PELSDAR+SW KN  
Sbjct: 422  NCQSIHREELNHLTRWVEEYRLDKLNFARQKQAYCYFSAAATLFPPELSDARISWAKNGV 481

Query: 1033 LSTVVDDFFDVKGSREELVNLINLVEKWECNTATNYCSEEVEIIFQALHKTVNDLGDIAF 854
            L+TVVDDFFDV GS EEL+NLI LVEKW+ N   + CSE VEIIF AL  T+N++G  AF
Sbjct: 482  LTTVVDDFFDVGGSEEELINLIQLVEKWDVNVGVDCCSENVEIIFSALKNTINEIGARAF 541

Query: 853  TRQGRSVRSHLIDIWLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGPVVLITQYF 674
            TRQGRSV SH+I+IWL L+KSM KE EW R+KS P MEEYMENA +SFALGP+VL   Y 
Sbjct: 542  TRQGRSVTSHVIEIWLDLIKSMFKEAEWLRNKSVPTMEEYMENAYVSFALGPIVLPALYL 601

Query: 673  LEPTLSKKVITSPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEYNKLFKHMNICCRL 494
            + P LS++ + S                                 E++ L++ M+   RL
Sbjct: 602  VGPKLSEEAVGS--------------------------------SEFDHLYRLMSTAGRL 629

Query: 493  LNDIQSFKREGEEGKLNSVLLCKIHSPEAINEQEAVREVKGIIDRNRRELLGLVLNTKGS 314
            LNDIQ FKRE  EGKLN+V L  IH    + E+EA+ E+K +I   RRELL LVL  KGS
Sbjct: 630  LNDIQGFKRESAEGKLNAVSLAMIHG-NGVTEEEAINEMKNVIVSKRRELLRLVLLEKGS 688

Query: 313  IVPKGCKDLFWKTFRVTHLFYREKDGFSSSLEMVNAVNAVIHKPLSL 173
            +VP+ CKDLFW   +V HLFY + DGF++  +M+  VNAV+ +P+ L
Sbjct: 689  VVPRACKDLFWNMSKVLHLFYAKHDGFTAH-DMMKTVNAVMEEPILL 734